Efficacy of GP-11 KHV DNA Vaccine in Common Carp (Cyprinus carpio) through Feed by Different Frequency of Administration

GP-11 KHV DNA vaccine is a vaccine that can be used to induce immunity against the KHV virus (Koi herpesvirus). Vaccination through feed is an alternative way of administering vaccines. The study aimed to examine the effect of giving KHV GP-11 DNA vaccine through feed with different frequencies to KHV infection. The frequency of vaccine administration is GP-11 vaccination once a week; GP-11(1x), GP-11 vaccination twice a week; GP-11(2x), GP-11 vaccination three times a week; GP-11(3x), GP-25 vaccinations three times a week; GP-25(3x), negative control (without KHV test) and positive control (KHV tested). The fish were kept for 28 days after vaccination and then continued with the KHV challenge test for 28 days. The weight of carp ranges from 13.82±2.37 g maintained with a density of 15 fish/aquarium. The results showed that vaccine treatment was able to induce an immune response as indicated by the number of white blood cells, lysozyme activity and post-vaccination antibody titer showed a significant effect compared to controls. Likewise, after the challenge test, supported by IFNγ and IgM gene expression parameters after the challenge test showed the highest value of vaccine treatment rather than control. The efficacy of vaccine was showed by RPS value (%) in each vaccine treatment obtained GP-11(1x) value of 44.7±3.7a, GP-11(2x) of 78.9±18.2b, GP-11(3x) 85.6±12.6b and GP-25(3x) 79.5±18.1b. It was concluded that administering the GP-11 vaccine frequency 2 times a week provides protection as strong as giving a vaccine frequency 3 times a week.Keywords: common carp, DNA vaccine, frequency of administration, koi herpesvirus

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Hematology of common carp following DNA vaccination and koi herpesvirus challenge test

Jurnal Akuakultur Indonesia ◽

10.19027/jai.9.9-15 ◽

2010 ◽

Vol 9 (1) ◽

pp. 9 ◽

Cited By ~ 2

Author(s):

Sri Nuryati ◽

N.A. Maswan ◽

. Alimuddin ◽

. Sukenda ◽

K. Sumantadinata ◽

...

Keyword(s):

Survival Rate ◽

Dna Vaccine ◽

Common Carp ◽

Cyprinus Carpio ◽

Lethal Dose ◽

Challenge Test ◽

Phagocytic Index ◽

Viral Glycoprotein ◽

Post Vaccination ◽

Koi Herpesvirus

The study was aimed to determine the effectiveness of DNA vaccine doses on hematological aspect which represent immune response and its influence on common carp survival rate. DNA vaccines encoding the viral glycoprotein of koi herpesvirus (KHV) have been proved to highly protect the fish under laboratory condition. A dose of 12.5 µg/100 µl vaccine had resulted in a survival rate of 96.67 % during 30 days after challenge test with a lethal dose of KHV. Fish vaccinated using lower doses, i.e. 2.5 and 7.5 µg/100µl showed 100% mortality after 15 and 19 days challenge test respectively, whereas non vaccinated fish as a control showed 100% mortality after 17 days challenge test. Total leucocytes of the vaccinated fish were higher than control until 42 days post vaccination, but declined afterward. Phagocytic index of the vaccinated fish using 12.5 µg/100 µl was declined after 49 days post vaccination or 7 days post challenge test. Key words: DNA vaccine, Koi herpesvirus (KHV), leucocyte, phagocytic index, Cyprinus carpio ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh vaksinasi menggunakan vaksin DNA dengan dosis berbeda terhadap gambaran darah ikan sebagai respresentasi tanggap kebal ikan mas serta pengaruhnya terhadap tingkat kelangsungan hidup ikan mas. Vaksin DNA penyandi glikoprotein koi herpesvirus (KHV) dapat memberikan proteksi yang tinggi pada percobaan skala laboratorium. Vaksinasi dengan dosis 12,5 µg/100µl dapat mempertahankan kelangsungan hidup sebesar 96,67% selama satu bulan setelah uji tantang dengan virus KHV menggunakan dosis letal. Ikan yang divaksin dengan dosis yang lebih rendah yaitu 2,5 dan 7,5 µg/100µl mengalami kematian total berturut-turut setelah 15 dan 19 hari uji tantang, sedangkan ikan kontrol yang tidak divaksin mengalami kematian total setelah 17 hari uji tantang. Jumlah leukosit total ikan yang divaksinasi lebih tinggi dibanding dengan kontrol sampai hari ke-42, setelah itu mengalami penurunan. Indeks fagositosis ikan yang divaksin dengan dosis 12,5 µg/100µl mengalami penurunan setelah hari ke-49 atau 7 hari setelah uji tantang. Kata kunci: Vaksin DNA, Koi herpesvirus (KHV), leukosit, indeks fagositosis, Cyprinus carpio

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Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion

Jurnal Akuakultur Indonesia ◽

10.19027/jai.11.76-85 ◽

2013 ◽

Vol 11 (1) ◽

pp. 76 ◽

Cited By ~ 2

Author(s):

Soko Nuswantoro ◽

. Alimuddin ◽

Munti Yuhana ◽

Ayi Santika ◽

Sri Nuryati ◽

...

Keyword(s):

Dna Vaccine ◽

Common Carp ◽

Polymerase Chain Reaction Analysis ◽

Dna Vaccination ◽

Chain Reaction ◽

Immersion Method ◽

Post Vaccination ◽

Koi Herpesvirus ◽

Polymerase Chain ◽

Fish Survival

Koi herpesvirus (KHV) is a herpesvirus that particularly infects and causes mass mortality to koi and common carp. Therefore, the protection of common carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine encoding KHV glycoprotein-25 by immersion method to increase survival of common carp against KHV infection. A total of 400 common carp juveniles at 30-day-old were immersed in 1-L water containing 1.3×108CFU/mL of the killed Escherichia coli cells carrying DNA vaccine. Three frequencies and three duration of fish immersion were tested, namely: 1×30 minutes, 1×60 minutes, 1× 90 minutes, 2×90 minutes and 3×90 minutes by interval of 24 hours. Reversetranscription polymerase chain reaction analysis showed that DNA vaccine was successfully expressed in the vaccinated fish. Fish at twenty eight days post vaccination were challenged by injecting 10-4 mL of KHV per fish. The result showed that vaccination by 1×30 minutes immersion allowed 61% of fish survived, and this was significantly higher (p<0.05) compared to control (without vaccination), but it was similar among vaccination treatments (p>0.05). The relative percent survival of vaccinated fish were also similar among treatments (p>0.05). DNA vaccination has increased fish survival about two fold higher compared to unvaccinated fish control (26.67%). Thus, DNA vaccination was effectively delivered by immersion for 1×30 minutes, and this technique can be useful to level up the resistance of common carp juveniles against KHV infection. Keywords: DNA vaccine, KHV, glycoprotein, immersion, common carp

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VAKSIN INAKTIF Klebsiella pneumoniae DENGAN PERLAKUAN SINAR GAMMA DAN PEMANASAN SUHU 65° C

Journal of Biological Researches ◽

10.23869/bphjbr.15.2.20109 ◽

2010 ◽

Vol 15 (2) ◽

pp. 151-157

Author(s):

I. Djajanegara ◽

I. Sugoro

Keyword(s):

Klebsiella Pneumoniae ◽

Blood Cells ◽

Vaccine Candidate ◽

White Blood Cells ◽

Challenge Test ◽

Positive Control ◽

Negative Control ◽

Cow Milk ◽

Coliform Bacteria ◽

Nuclear Technique

Klebsiella pneumoniae is one of coliform bacteria which cause human dan mammalian diseases. The bacteria dominate in dairy cow milk which has suffered from mastitis and has resistent on antibiotic. Vaccination is one of aims to prevent the diseases. Nuclear technique could be used to have a vaccine candidate. This research was conducted to get the influence of inactivated K. pneumoniae by gamma irradiated and heat inactivated as vaccine candidate on mice. The treatment were positive control (infected by K. pneumoniae), Negative control (injection by physiologies NaCl solution), 800 Gy (infected by K. pneumoniae has inactivated with 800 Gy), 1000 Gy (infected by K. pneumoniae has inactivated with 1000 Gy), 30’ (infected by K. pneumonia has inactivated with heat 65° C for 30’), and 45’ (infected by K. pneumoniae has inactivated with heat 65° C for 30’). The parameters were physical condition, weight, organ, total number of red, white blood cells, and intraperitoneal macrophage. The results showed that irradiated vaccines were better than heat. % mortality of positive control was 100% but the treatments were 0%. The physic condition of mice was normal for irradiated treatment, but not for negative control and heat treatment. The weight gain of mice after vaccination and challenge test were decreased and back normal after 2 days. The organ condition of mice was normal after vaccination and challenge test. The total number of red and white blood cells showed the same pattern for all treatment after vaccination and challenge test, but the total of intraperitoneal macrophage was increased after 4 hours vaccination. Based on the results showed that irradiated vaccine has potential to develop as vaccine candidate.

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EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio koi

Indonesian Aquaculture Journal ◽

10.15578/iaj.11.1.2016.31-39 ◽

2016 ◽

Vol 11 (1) ◽

pp. 31

Author(s):

Sekar Ayu Chairunnisa ◽

Sri Nuryati ◽

Alimuddin Alimuddin ◽

Sri Murtini ◽

Ayi Santika ◽

...

Keyword(s):

Dna Vaccine ◽

Positive Control ◽

Negative Control ◽

Economic Losses ◽

Koi Herpesvirus ◽

Naked Dna ◽

Group 4 ◽

Dead Fish ◽

Group 2 ◽

Cyprinus Carpio Koi

Koi herpesvirus (KHV) is one of the major pathogen for koi and common carp which cause high mortality and economic losses for the farmer. The purpose of this study was to determine the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25 KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by intramuscularly injection to the 3-month-old koi. The fish were divided into six groups, i.e. unvaccinated group (negative control C- and positive control C+), and vaccinated group (2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5 μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42 post vaccination, all fish of each groups were challenged by injecting KHV titre 10-3 FID50. Number of dead fish was counted everyday after the challenge until 30 days. The results showed that vaccinated fish were had survival rate of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA vaccine for preventing KHV infection.

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In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2021/v23i930256 ◽

2021 ◽

pp. 12-20

Author(s):

Udeme O. Georgewill ◽

Festus Azibanigha Joseph ◽

Elias Adikwu

Keyword(s):

Plasmodium Berghei ◽

Blood Cells ◽

Hematological Parameters ◽

White Blood Cells ◽

Positive Control ◽

Negative Control ◽

Antiplasmodial Activity ◽

Significant Difference ◽

Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite. NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

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APLIKASI SINBIOTIK UNTUK PENCEGAHAN INFEKSI INFECTIOUS MYONECROSIS VIRUS PADA UDANG VANAME (Litopenaeus vannamei) (Synbiotic Application for Prevention of Infectious Myonecrosis Virus Infection in White Shrimp (Litopenaeus vannamei))

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences ◽

10.21157/j.ked.hewan.v10i2.5041 ◽

2016 ◽

Vol 10 (2) ◽

pp. 121-127

Author(s):

Widanarni Widanarni ◽

Sukenda Sukenda ◽

Ghita Ryan Septiani

Keyword(s):

Survival Rate ◽

Immune Responses ◽

Litopenaeus Vannamei ◽

Ipomoea Batatas ◽

Challenge Test ◽

Positive Control ◽

Negative Control ◽

White Shrimp ◽

Infectious Myonecrosis Virus ◽

Synbiotic Supplementation

This study aimed to evaluate the effectiveness of dietary synbiotic at different giving frequencies on growth, immune responses, and resistance of white shrimp infected by infectious myonecrosis virus (IMNV). Synbiotic used in this study was combination of probiotic Vibrio alginolyticus SKT-b and prebiotic oligosaccharides extracted from sweet potatoe (Ipomoea batatas L). Doses of probiotic and prebiotic used were 1% and 2% (w/w), respectively. The white shrimps (0.493±0.035 g) were divided into five treatments consisting of A and B (without supplementation of synbiotic: (A) positive control; (B) negative control), C (daily synbiotic supplementation), D (twice a week synbiotic supplementation), and E (weekly synbiotic supplementation). After 30 days of feeding trial, white shrimps were infected by IMNV (except negative control). The results showed that daily growth rate of white shrimp on all synbiotic treatments (C, D, and E) ranged from 6.93±0.025-6.97±0.019% and had higher values than controls (A and B) (P<0.05). Meanwhile, feed conversion value in C and D (1.54±0.142 and 1.58±0.117) were lower than controls (P<0.05). Supplementation of synbiotic with different frequencies also affected survival rate of white shrimp after the challenge test with IMNV; daily synbiotic supplementation (C) resulted in a 50% higher survival rate than positive control (P<0.05). This was associated with immune responses parameters values of synbiotic treatment (before and after the challenge test) which were better than positive control. In conclusion the addition of synbiotic in feed resulted in higher growth performances, immune responses,and resistance of white shrimp to IMNV infection.

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CONTROL OF Vibrio alginolyticus INFECTION IN ASIAN SEA BASS Lates calcarifer USING AMBON BANANA PLANT POWDER Musa paradisiacal THROUGH THE FEED

Indonesian Aquaculture Journal ◽

10.15578/iaj.15.2.2020.85-91 ◽

2021 ◽

Vol 15 (2) ◽

pp. 85

Author(s):

Hamida Pattah ◽

Dinamella Wahjuningrum ◽

Munti Yuhana ◽

Widanarni Widanarni

Keyword(s):

Challenge Test ◽

Sea Bass ◽

Positive Control ◽

Negative Control ◽

Vibrio Alginolyticus ◽

Feed Consumption ◽

Banana Plant ◽

Asian Sea Bass ◽

Antibiotic Control ◽

Lower Production

Asian sea bass is one of economically important commodities in aquaculture. However, its culture often challenged by vibriosis infection which resulted in lower production. The study aimed to evaluate the effectiveness of the ambon banana plant powder application in feed as an effort to control the disease caused by Vibrio alginolyticus infection. Asian sea bass with total length of 7.48 ± 0.45 cm. Five different treatments were applied in this experiment, i.e. positive control, negative control, prevention, curative, and antibiotic application. The challenge test is carried out on the 15th day. Challenge test was performed with intramuscularly injection of V. alginolyticus at the cell density of 106 CFU/fish. The results showed the amount of feed consumption after the challenge test was highest in the curative and antibiotic control. However, these results were not significantly different (P>0.05) among preventive and curative treatments but were significantly different from controls (P<0.05). Fish treated with supplemented feed showed the highest total erythrocytes, total leukocytes, hemoglobin, respiratory burst, and lysozyme activity compared to controls. IL-1b gene expression increased after the challenge test with the highest level of expression in the curative treatment. It can be concluded that the administration of ambon banana plant powder (3 g/100 g diet) could control of V. alginolyticus infection in Asian sea bass and resulted 70.00% survival rate on prevention and 83.33% on curative treatments.

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Effication of Viral Nervous Necrosis DNA as Vaccines for Cromileptes altivelis

Indonesian Journal of Tropical Aquatic ◽

10.22219/ijota.v3i2.13891 ◽

2020 ◽

Vol 3 (2) ◽

Author(s):

Wiwien Mukti Andriyani ◽

Sri Murtini ◽

Alimuddin Alimuddin

Keyword(s):

Dna Vaccine ◽

Challenge Test ◽

Virus Titer ◽

Dna Vaccination ◽

Post Vaccination ◽

Viral Nervous Necrosis ◽

Antibody Induction ◽

Actin Promoter ◽

Two Stages ◽

Made In

Viral nervous necrosis (VNN) is a disease that often infects groupers. It has caused mass death in more than 34 species of marine fish. DNA vaccination might become a solution againts the infection. The construction of the pmBA-CP DNA vaccine consisting of the beta-actin promoter of medaka fish (Oryzias latipes) and capsid protein (CP) encoding VNN RNA2 has been made in previous studies. This study aimed to test the efficacy of the pmBA-CP DNA vaccine for VNN. The experiment consisted of two stages, namely (1) detection of anti-VNN antibody induction in vaccinated fish using ELISA, and (2) challenge test for fish vaccinated with the VNN virus. Grouper (body length 8 cm to 10 cm) were divided into two groups with a density of 5 fish 60 L–1. The fish in the first group were vaccinated with pmBA-CP intramuscularly at a dose of 12.5 µg per fish, while the second group of fish were not vaccinated. Antibody titer testing was carried out before treatment, and 1 d, 7 d, 14 d, 21 d, 28 d, and 35 d after vaccination. The challenge test was carried out on the 60th day after vaccination. The results showed that the S / P ratio in the vaccinated fish serum was higher than unvaccinated fish at 21 d to 35 d post-vaccination. DNA vaccination was able to induce anti-VNN antibodies of grouper. The results of the challenge test for vaccinated fish using VNN virus titer 103.5 FID50/0.2 mL showed 60% of survival rate. Thus, the pmBA-CP DNA vaccine could be useful for increasing grouper immunity, and support production of grouper.

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Frequency and persistency of DNA vaccine encoding GP25 by oral on common carp

Jurnal Akuakultur Indonesia ◽

10.19027/jai.12.150-157 ◽

2015 ◽

Vol 12 (2) ◽

pp. 150

Author(s):

Sri Nuryati ◽

, Yuliyanti ◽

, Alimuddin

Keyword(s):

Escherichia Coli ◽

Dna Vaccine ◽

Common Carp ◽

Oral Delivery ◽

Culture Medium ◽

Dose Frequency ◽

Viral Pathogen ◽

Koi Herpesvirus ◽

Pcr Method ◽

Almost All

ABSTRACT Koi herpesvirus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak is happened in almost all centre of common carp culture in Indonesia and caused mass mortality. Deoxyribonucleic acid (DNA) vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. The aim of this research was to get frequency and persistency of DNA vaccine encoding GP25 given by oral delivery method in common carp. This research would like to determine dose, frequency of vaccination, persistency of DNA vaccine and culture medium for the bacterial host. DNA vaccine persistency test was done by using polymerase chain reaction (PCR) method with the specific primer for GP25 gene. The results showed that level of DNA vaccine that could be detected in feed was 7.56 ng (equal to 1.598×1010 copies). Efficient culture medium for Escherichia coli DH5α carrying DNA vaccine was LB triptone. Feeding fish with diet supplemented with 1 mL E. coli DH5α containing DNA vaccine for each fish and two times a week allowed persistence of DNA vaccine in kindney and spleen. Keywords: common carp, KHV, DNA vaccine, GP25, persistance ABSTRAK Koi herpesvirus (KHV) adalah virus patogen utama yang menginfeksi ikan mas dan ikan koi. Wabah penyakit KHV terjadi di hampir semua sentra budidaya ikan mas di Indonesia dan menyebabkan kematian massal ikan. Metode vaksinasi DNA merupakan salah satu cara yang dapat dilakukan untuk menanggulangi serangan KHV. Pemberian vaksin umumnya dilakukan dengan cara injeksi. Tujuan penelitian ini adalah untuk menguji frekuensi dan persistensi vaksin DNA GP25 antivirus KHV yang diberikan melalui oral pada ikan mas. Pada penelitian ini dilakukan uji dosis, frekuensi pemberian vaksin, persistensi vaksin DNA, dan media kultur bakteri inang. Persistensi vaksin DNA dianalisis menggunakan metode PCR dengan primer spesifik gen GP25. Hasil penelitian menunjukkan bahwa dosis vaksin DNA yang dapat terdeteksi dalam pakan adalah 7,56 ng (setara dengan 1,598×1010 copy). Media kultur yang efisien bagi bakteri Escherichia coli DH5α pembawa vaksin DNA adalah LB tripton. Pemberian pakan bervaksin berupa bakteri konsentrasi 1 mL/ekor ikan dengan frekuensi dua kali seminggu menghasilkan persistensi DNA GP25 di ginjal dan limpa. Kata kunci: ikan mas, KHV, vaksin DNA, GP25, persistensi

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TRANSMISI GEN krt-GP11 DAN PERFORMA KETAHANAN IKAN MAS (Cyprinus carpio) TRANSGENIK F-2 TERHADAP INFEKSI KHV

Jurnal Riset Akuakultur ◽

10.15578/jra.11.4.2016.297-305 ◽

2017 ◽

Vol 11 (4) ◽

pp. 297

Author(s):

Khairul Syahputra ◽

Flandrianto Sih Palimirmo ◽

Yogi Himawan

Keyword(s):

Common Carp ◽

Challenge Test ◽

Specific Primer ◽

Breeding Programs ◽

Koi Herpesvirus ◽

F2 Population ◽

Fish Breeding ◽

Pcr Method ◽

Transgenic Male ◽

Transgene Transmission

Pembentukan ikan mas transgenik merupakan salah satu program penelitian di Balai Penelitian Pemuliaan Ikan, Sukamandi dalam rangka menghasilkan varietas unggul ikan mas tahan infeksi KHV (Koi herpesvirus). Pada tahun 2015 telah dilakukan pembentukan ikan mas transgenik tahan KHV generasi F-2. Penelitian ini bertujuan untuk mengevaluasi transmisi gen krt-GP11, ketahanan ikan mas transgenik F-2 terhadap infeksi KHV, keberadaan marka Cyca-DAB1*05 tahan KHV pada populasi ikan mas transgenik F-2. Ikan mas transgenik F-2 dihasilkan dengan memijahkan ikan mas transgenik F-1 jantan dengan betina non-transgenik. Pengujian transmisi transgen dan deteksi marka ketahanan KHV pada transgenik F-2 dilakukan dengan metode PCR menggunakan primer spesifik untuk transgen krt-GP11 dan gen Cyca-DAB1*05. Evaluasi ketahanan ikan mas transgenik F-2 terhadap infeksi KHV dilakukan dengan uji tantang secara kohabitasi. Hasil penelitian menunjukkan bahwa transmisi gen krt-GP11 pada keturunan F-2 memiliki persentase yang relatif rendah yaitu sebesar 0%-2%. Ikan mas transgenik F-2 memiliki ketahanan relatif baik terhadap KHV dengan sintasan uji tantang sebesar 90% dan tidak berbeda nyata dengan ikan mas pembanding atau non-transgenik (P>0,05). Tingginya pesentase keberadaan marka Cyca-DAB1*05 pada populasi transgenik berperan pada ketahanan ikan mas transgenik terhadap infeksi KHV.Creating of transgenic common carp is one of the breeding programs in Research Institute for Fish Breeding for producing a superior strain of common carp resistant to KHV(Koi herpesvirus). Since 2015, the creation of common carp transgenic has been conducted to produce F2 population resistant to KHV. This study was aimed to evaluate the transmission of krt-GP11 gen,the resistantce of F2 transgenic common carp against to KHV infection, and the existence of Cyca-DAB1*05 marker resistant to KHV in F2 transgenic population. F2 transgenic population has been produced by mating F1 transgenic male with non transgenic female. Transgene transmission and the existence of marker resistant to KHV in F2 transgenic population were evaluated by PCR method using specific primer to krt-GP11 gene and Cyca-DAB1*05 gene, respectively. The resistance of F2 transgenic population againstto KHV infection was evaluated by challenge test using cohabitation method. The result showed that transmission of krt-GP11 gene in F2transgenic population was relatively low with percentage of 0-2%. The resistance of F2 transgenic common carp against to KHV was relatively high with survival rate of 90% and was not significantly different from non transgenic (p>0.05). High percentage of transgenic population having Cyca-DAB1*05 marker had a role in resistance of transgenic population against KHV infection.

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