scholarly journals Fermentation of Soybean Seeds Using Rhizopus oligosporus for Tempeh Production and Standardization Based on Isoflavones Content

Author(s):  
Yohanes Dwiatmaka ◽  
Endang Lukitaningsih ◽  
Nunung Yuniarti ◽  
Subagus Wahyuono

Tempeh is an Indonesian traditional food, made from white soybean seeds by fermentation with Rhizopus mold. Soybean seeds content high isoflavone glycosides. Isoflavones can act as phytoestrogen, antioxidant and improves memory. Isoflavone glycosides are poorly absorbed in the human body, but the aglycone isoflavones absorbed quickly. Rhizopus mold hydrolize isoflavone glycosides to be aglycone isoflavones during tempeh production. This research was aimed to know the best room temperature and time of fermentation for making soybean tempeh with high content of isoflavones. After rinsing and boiling, the soybean seeds than fermented with Rhizopus oligosporus. Three conditions were applied: (a) ambient temperature (27-32oC) without air circulation, (b) 27±0.5 oC, and (c) 30±0.5 oC both with air circulations. Inner temperature of tempeh was recorded hourly. Total isoflavones were measured with UV spectrophotometer every 6 hours. Based on this study, fermentation at condition a caused the tempeh too hot and rotted quickly. Fermentation at condition b and c produced tempeh with good quality. Tempeh ripening was reached between 31-32 hours with inner temperature 32-33 oC. Fermentation for 72 hours at condition b was chosen because its high total isoflavones content (0.089% w/w), but decrease about 20% compare to soybean seeds (0.112% w/w).

Author(s):  
Yohanes Dwiatmaka ◽  
Endang Lukitaningsih ◽  
Nunung Yuniarti ◽  
Subagus Wahyuono

Tempeh is an Indonesian traditional food, made from white soybean seeds by fermentation with Rhizopus mold. Soybean seeds content high isoflavone glycosides. Isoflavones can act as phytoestrogen, antioxidant and improves memory. Isoflavone glycosides are poorly absorbed in the human body, but the aglycone isoflavones absorbed quickly. Rhizopus mold hydrolize isoflavone glycosides to be aglycone isoflavones during tempeh production. This research was aimed to know the best room temperature and time of fermentation for making soybean tempeh with high content of isoflavones. After rinsing and boiling, the soybean seeds than fermented with Rhizopus oligosporus. Three conditions were applied: (a) ambient temperature (27-32oC) without air circulation, (b) 27±0.5 oC, and (c) 30±0.5 oC both with air circulations. Inner temperature of tempeh was recorded hourly. Total isoflavones were measured with UV spectrophotometer every 6 hours. Based on this study, fermentation at (a) condition caused the tempeh too hot and rotted quickly. Fermentation at (b) and (c) conditions produced tempeh with good quality. Tempeh ripening was reached between 32-32 hours with inner temperature 32-33oC. Fermentation for 72 hours at condition (b) was chosen because its high total isoflavones content (0.089% w/w), but decrease about 20% compare to soybean seeds (0.112% w/w).


Author(s):  
S. Mahajan

The evolution of dislocation channels in irradiated metals during deformation can be envisaged to occur in three stages: (i) formation of embryonic cluster free regions, (ii) growth of these regions into microscopically observable channels and (iii) termination of their growth due to the accumulation of dislocation damage. The first two stages are particularly intriguing, and we have attempted to follow the early stages of channel formation in polycrystalline molybdenum, irradiated to 5×1019 n. cm−2 (E > 1 Mev) at the reactor ambient temperature (∼ 60°C), using transmission electron microscopy. The irradiated samples were strained, at room temperature, up to the macroscopic yield point.Figure 1 illustrates the early stages of channel formation. The observations suggest that the cluster free regions, such as A, B and C, form in isolated packets, which could subsequently link-up to evolve a channel.


2011 ◽  
Vol 10 (4) ◽  
pp. 511-517 ◽  
Author(s):  
Calin Corciova ◽  
Radu Ciorap ◽  
Dan Zaharia ◽  
Alexandru Salceanu

RSC Advances ◽  
2021 ◽  
Vol 11 (41) ◽  
pp. 25731-25737
Author(s):  
Maria Cristina Righetti ◽  
Maria Laura Di Lorenzo ◽  
Patrizia Cinelli ◽  
Massimo Gazzano

At room temperature and at the human body temperature, all the amorphous fraction is mobile in poly(butylene succinate).


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Liliana G. Fidalgo ◽  
Mário M. Q. Simões ◽  
Susana Casal ◽  
José A. Lopes-da-Silva ◽  
Ivonne Delgadillo ◽  
...  

AbstractHyperbaric storage at room temperature (HS/RT: 75 MPa/25 °C) of vacuum-packaged fresh Atlantic salmon (Salmo salar) loins was studied for 30 days and compared to atmospheric pressure at refrigerated temperatures (AP/5 °C, 30 days) and RT (AP/25 °C, 5 days). Most of the fatty acids were not affected by storage conditions, with only a slight decrease of docosahexaenoic acid (DHA) content (n-3 polyunsaturated fatty acid) for AP samples, reflected in the lower polyene index values obtained and higher oxidation extent. For HS, a lower lipid oxidation extension and a slower increase of myofibrillar fragmentation index values were observed, when compared to AP samples. The volatile profile was similar for the HS and fresh samples, with the HS samples retaining fresh-like alcohols and aldehydes components, which disappeared in AP samples, mainly in AP/25 °C samples. The volatile profile for AP samples (5 and 25 °C) revealed mostly spoilage-like compounds due to microbial activity. Drip loss increased progressively during the 30 days of storage under HS, while a slight decrease of water holding capacity after 5 days was observed, increasing further after 30 days. Regarding textural properties, only resilience was affected by HS, decreasing after 30 days. So, HS/RT could represent an interesting extended preservation methodology of fresh salmon loins, since allows retaining important physicochemical properties for at least 15 days, while refrigeration after 5 days showed already volatile spoilage-like compounds due to microbial activity. Furthermore, this methodology allows additional considerable energy savings when compared to refrigeration.


2021 ◽  
Vol 105 (1) ◽  
pp. 119-134
Author(s):  
Jana Zimáková ◽  
Petr Baca ◽  
Martin Langer ◽  
Tomáš Binar

This work deals with lead-acid batteries, their properties and individual types that are available on the market. The temperature dependences of the battery parameters at different ambient temperatures and at different discharging and charging modes are measured. 6 batteries are tested at different charging currents, which provides information about their behavior both during discharge and at the time of charging. During the experiments, testing is not only performed at room temperature, but the batteries are also exposed to high temperatures up to 75 °C.


2019 ◽  
pp. 57-68

“Solo” papaya fruits were harvested in October, 2016 & 2017 seasons from a commercial orchard located in Ismailia Governorate, Egypt. Papaya fruits were harvested at three maturity stages: 25% yellow (stage 1), 50% yellow (stage 2) and 100% yellow (stage 3) and evaluated during storage at ambient temperature (20°C ± 2) for 4 days + at 80- 85% RH or during cold storage at 6°C + 90- 95% RH for 20 days. Papaya fruits softened very rapidly at room temperature after harvest and had 4 days shelf life. However, the fruit can be stored for 20 days at 6°C with little changes in firmness and the fruit apparently progressed in normal ripening upon removal to ambient temperature (20°C) for 3 days. All colour values (a*, L* and C*) were linearly increased during cold storage. Conversely, as a result of colour change from green to orange-red, h° values decreased. Soluble solids content was not affected during ripening at 20°C and remained steady. Fruit harvested at stage 2 and stored at 6°C for 20 days following 3 days at 20°C had superior score for sensorial evaluation.


2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Ramadan Ahmed Mekheimer ◽  
Abdullah Mohamed Asiri ◽  
Afaf Mohamed Abdel Hameed ◽  
Reham R. Awed ◽  
Kamal Usef Sadek

AbstractStarting from readily available 2-naphthol, aldehydes, aryl and alkylamines, a variety of Betti bases were efficiently synthesized utilizing a catalytic amount of cerium (IV) ammonium nitrate (CAN) at room temperature. This protocol has advantages of high yield, mild reaction conditions, no environmental pollution, diversity of reactants and simple work up procedure.


Plant Disease ◽  
2021 ◽  
Author(s):  
Jennifer Juzwik ◽  
Marc A Hughes ◽  
Zhanghing Chen ◽  
Anna Yang ◽  
Lisa Keith ◽  
...  

A new and devastating disease, rapid ohia death (ROD), in Hawaii led to a state quarantine that regulates inter-island transport of ohia wood and plant material to prevent spread of the causal pathogens. Heat treatments of ohia logs in commercial trade were considered for phytosanitary treatment. Vacuum steam (VS) was evaluated for its ability to eradicate the pathogens, Ceratocystis lukuohia and/or C. huliohia, in main stem logs from ROD-affected forest trees. Replicate loads of three de-barked logs (24 to 43 cm diameter; 1.7 to 2.0 m long) were VS-treated at 56° C for 30 min (5 loads) or 60° C for 60 min (4 loads) at a sapwood depth equal to 70% of log radius. Percent isolation of Ceratocystis from VS and ambient temperature logs prior to treatment and summarized by source tree ranged from 12 to 66% and 6 to 31% based on carrot baiting assays of tissue taken from outer and inner sapwood, respectively. No viable Ceratocystis was detected in either sapwood locations for the 60° C/ 60 min schedule or for the inner locations for the 56° C/ 30 min schedule following treatment. Only one subsample (0.48%, n=208) of the latter schedule treatment yielded Ceratocystis. Time required for treatment ranged from 7.4 to 15 h for the 56° C/ 30 min schedule and from 8.6 to 19.2 h for the 60° C/ 60 min schedule. These results demonstrate VS is an effective and efficient method for treating large diameter ohia logs that mill owners and regulatory plant pathologists may consider for use in Hawaii.


Synthesis ◽  
2022 ◽  
Author(s):  
Rekha Bai ◽  
Kamlesh Kumar Dabaria ◽  
Satpal Singh Badsara

A metal-free direct C-H selenation of aniline derivatives via an iodine catalysed C-Se bond formation using diselenides as a selenium source at ambient temperature is described. A variety of aniline derivatives underwent regio-selective C-H selenation with different diselenides to afford the corresponding aryl selenoethers in good to excellent yields.


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