scholarly journals Effects of Bradyrhizobium japonicum on Nitrogen Content in Soybean Leaves and Seeds Cultivated on Acidic Soils

2021 ◽  
Author(s):  
Ben Tshibuyi Kasu-Bandi ◽  
Fabien Kitengie Kitengie ◽  
Jonas Lwamuguma Bagaluza ◽  
Aristote Bwende Nkolomini ◽  
Emery Kasongo Lenge ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Tropical Region ◽  
Acidic Soils ◽  
N Content ◽  
Total N ◽  
N Assimilation ◽  
Uninoculated Control ◽  
Soybean Plants ◽  
Different Strains ◽  
Soybean Leaves

The legume-rhizobium symbiosis plays an important role in the nitrogen (N) assimilation of plants, more particularly in the humid tropical region where soils are deeply weathered and have poor electrochemical properties. On acidic soils of Haut-Katanga, DR Congo it is not clear how application of Bradyrhizobium japonicum affect N allocation among soybean organs (e.g., leaves, seeds). Here, we assessed the effect of Bradyrhizobium japonicum on N content of soybean leaves and seeds cultivated on acidic soils. We conducted two experiments using a split-plot setup with three replicates in two sites (i.e., Kasapa and Kanyameshi). The main plots included three strains of Bradyrhizobium plus the uninoculated control and four varieties of soybean in the subplot. We found that the different strains of Bradyrhizobium did not induce significant effects on the total N content of soybean leaves and seeds in the Kasapa site. In contrast, Bradyrhizobium affected significantly the N content of soybean leaves in the Kanyameshi site. We demonstrated that N content in the soil, which varies between the two sites, positively influences yield and nodulation. We conclude that that the efficiency of the soybean-Bradyrhizobium symbiosis and its influence on the allocation of N through soybean plants strongly depend on the chemical characteristics of the soil and particularly on the initial levels of N in the soils.

scholarly journals Biocompatibility Effect of Bradyrhizobium japonicum and Trichoderma Strains on Growth, Nodulation and Physiological Traits of Soybean (Glycine max L.) under Water Deficit Conditions

2020 ◽  
pp. 52-66
Author(s):  
Sahar El- Nahrawy ◽  
Mohssen Elbagory ◽  
Alaa El-Dein Omara
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Block Design ◽  
Soluble Sugar ◽  
Trichoderma Viride ◽  
Dry Weight ◽  
Physiological Traits ◽  
Control Treatment ◽  
N Content ◽  
Total Soluble Sugar ◽  
Soybean Plants

In the future, drought are expected to increase, affecting the productivity of crops sensitive to water scarcity. Through N2-fixation process, soybean is capable of achieving its nitrogen demands, however, this process is inhibited under drought stress conditions. Therefore, it is vital to find suitable solutions for the agricultural sustainability of soybean. Under pot experiment, biocompatibility was studied between Bradyrhizobium japonicum and Trichoderma strains (Trichoderma viride, T. harzianum and T. kongii) for their ability to stimulate the growth, nodulation, N content and photosynthetic pigments of soybean plants under different irrigation intervals (every 2 days (I1), every 4 days (I2), and every 6 days (I3)). The experiment was conducted in summer 2020 with a split-plot randomized complete block design and six replicates. Among the Trichoderma strains, T. harzianum was the most tolerant to growth and auxin production in the maximum 25% PEG 6000 (poly ethylene glycol) concentration. Also, co-inoculation treatment (irrigation every 6 days and inoculation with B. japonicum + T. harzianum) recorded an increase rate reached to 69.4% for shoot length, 102.53% for root length, 79.06% for shoot dry weight, 103.44% for root dry weight and 178.57% for N content compared to control treatment. For physiological traits (chlorophyll a, b, carotenoids and total soluble sugar), there was a significant increase was observed when soybean plants inoculated with B. japonicum + T. harzianum treatment followed by B. japonicum + T. kongii treatment, under irrigation every 6 days condition. On the contrary, a decrease was observed in proline content for the same treatments. Thus, an adequate microbial consortium of Bradyrhizobium - Trichoderma, could represent a promising practical method for increasing the productivity of soybean especially when grown under drought conditions.

Symbiotic Effectivity of Four Phaseolus vulgaris Genotypes after Inoculation with Different Strains of Rhizobium under Controlled Conditions

1994 ◽  
Vol 49 (5-6) ◽  
pp. 343-351 ◽  
Author(s):  
Bernhard Epping ◽  
Alexander P. Hansen ◽  
Bahman Djalali ◽  
Peter Martin
Keyword(s):  
N Uptake ◽  
Dry Weight ◽  
Individual Plant ◽  
Mineral N ◽  
N Content ◽  
Total N ◽  
Controlled Conditions ◽  
Reducing Activity ◽  
Acetylene Reducing Activity ◽  
Different Strains

Abstract Four varieties of P. vulgaris L. were tested for their symbiotic nitrogen fixation effectivity in combination with nine different strains of Rhizobium leguminosarum bv. phaseoli and Rhizobium tropici. Plants were grown under controlled conditions and harvested 23 days after planting. Acetylene reducing activity, total N-content and dry weight of individual plant components were determined. Significant differences due to plant x bacterium interaction were assessed by ANOVA, especially for the total nodule mass per plant and the acetylene reducing activity per nodule dry weight. Data for acetylene reducing activity per plant correlated highly with the corresponding data for the total N-content. The comparison of the total N-content in symbiotically grown plants, lacking supply of mineral N, with plants luxuriously supplied with mineral N (relative N-accumulation rate) revealed high values (between 60% and 70% of maximal N-uptake) for some symbiotically active plant/bacterium combinations for this early developmental stage of the symbiosis N2 fixation potential for such symbioses.

scholarly journals Biochar and urease inhibitor mitigate NH3 and N2O emissions and improve wheat yield in a urea fertilized alkaline soil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Khadim Dawar ◽  
Shah Fahad ◽  
M. M. R. Jahangir ◽  
Iqbal Munir ◽  
Syed Sartaj Alam ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Grain Yield ◽  
Block Design ◽  
N Uptake ◽  
Nitrous Oxide Emissions ◽  
N2o Emissions ◽  
Urease Inhibitor ◽  
Alkaline Soil ◽  
Total N ◽  
N Assimilation

AbstractIn this study, we explored the role of biochar (BC) and/or urease inhibitor (UI) in mitigating ammonia (NH3) and nitrous oxide (N2O) discharge from urea fertilized wheat cultivated fields in Pakistan (34.01°N, 71.71°E). The experiment included five treatments [control, urea (150 kg N ha−1), BC (10 Mg ha−1), urea + BC and urea + BC + UI (1 L ton−1)], which were all repeated four times and were carried out in a randomized complete block design. Urea supplementation along with BC and BC + UI reduced soil NH3 emissions by 27% and 69%, respectively, compared to sole urea application. Nitrous oxide emissions from urea fertilized plots were also reduced by 24% and 53% applying BC and BC + UI, respectively, compared to urea alone. Application of BC with urea improved the grain yield, shoot biomass, and total N uptake of wheat by 13%, 24%, and 12%, respectively, compared to urea alone. Moreover, UI further promoted biomass and grain yield, and N assimilation in wheat by 38%, 22% and 27%, respectively, over sole urea application. In conclusion, application of BC and/or UI can mitigate NH3 and N2O emissions from urea fertilized soil, improve N use efficiency (NUE) and overall crop productivity.

A study of root and shoot interactions between cereals and peas in mixtures

1993 ◽  
Vol 120 (1) ◽  
pp. 13-24 ◽  
Author(s):  
M. P. Tofinga ◽  
R. Paolini ◽  
R. W. Snaydon
Keyword(s):  
Relative Yield ◽  
Root Competition ◽  
Total Biomass ◽  
N Content ◽  
Total N ◽  
Resource Complementarity ◽  
Relative Yield Total ◽  
Shoot Competition ◽  
Competitive Abilities ◽  
Different Sources

SUMMARYWheat, barley and two morphologically contrasting cultivars of peas (leafy and semi-leafless) were grown in pure stands, at standard agricultural densities, and in additive mixtures of cereals with peas. The stands were grown in boxes in the field, and partitions were used to separate the effects of root and shoot interactions. The cereals and peas were either planted at the same time, or one species was planted 10 days before the other. The origin of the N present in each species was determined by applying N fertilizer labelled with 15N.Both cultivars of peas had greater shoot and root competitive abilities than wheat or barley, probably because of their larger seed size; leafy peas had greater shoot and root competitive abilities than semi-leafless peas. Sowing peas after cereals reduced their competitive ability.The relative yield total (RYT) of cereal-pea mixtures, based on total biomass, averaged 1·6 when only the root systems interacted, and 1·4 when only the shoot systems interacted, but did not differ significantly from 10 when both root and shoot systems interacted. RYT values were greater when peas were grown with wheat, rather than with barley, and when peas were sown at the same time as the cereals.Shoot competition from peas increased the N% of cereals, but substantially reduced their total N content, because biomass yield was reduced. Shoot competition from cereals had no effect on the N% of peas, and only slightly reduced their total N content. Shoot competition between cereals and peas had no significant effect upon the proportion of N derived from various sources by either cereals or peas.Root competition from peas significantly reduced both the N% and total N content of cereals. Root competition from cereals had little effect on the N% of peas, but significantly reduced their total N content and increased the proportion of N derived from rhizobial fixation from 76 to 94%. Since cereals and peas largely used different sources of N, resource complementarity for N was probably an important component of intercropping advantage, when the roots of cereals and peas shared soil resources.

scholarly journals Seasonal variation in nitrogen pools and <sup>15</sup>N/<sup>13</sup>C natural abundances in different tissues of grassland plants

2012 ◽  
Vol 9 (5) ◽  
pp. 1583-1595 ◽  
Author(s):  
L. Wang ◽  
J. K. Schjoerring
Keyword(s):  
Seasonal Pattern ◽  
Exchange Potential ◽  
Total N ◽  
Δ13c Values ◽  
Green Leaves ◽  
Nh3 Emission ◽  
N Concentration ◽  
Grassland Plants ◽  
N Assimilation ◽  
Different Tissues

Abstract. Seasonal changes in nitrogen (N) pools, carbon (C) content and natural abundance of 13C and 15N in different tissues of ryegrass plants were investigated in two intensively managed grassland fields in order to address their ammonia (NH3) exchange potential. Green leaves generally had the largest total N concentration followed by stems and inflorescences. Senescent leaves had the lowest N concentration, indicating N re-allocation. The seasonal pattern of the Γ value, i.e. the ratio between NH4+ and H+ concentrations, was similar for the various tissues of the ryegrass plants but the magnitude of Γ differed considerably among the different tissues. Green leaves and stems generally had substantially lower Γ values than senescent leaves and litter. Substantial peaks in Γ were observed during spring and summer in response to fertilization and grazing. These peaks were associated with high NH4+ rather than with low H+ concentrations. Peaks in Γ also appeared during the winter, coinciding with increasing δ15N values, indicating absorption of N derived from mineralization of soil organic matter. At the same time, δ13C values were declining, suggesting reduced photosynthesis and capacity for N assimilation. δ15N and δ13C values were more influenced by mean monthly temperature than by the accumulated monthly precipitation. In conclusion, ryegrass plants showed a clear seasonal pattern in N pools. Green leaves and stems of ryegrass plants generally seem to constitute a sink for NH3, while senescent leaves have a large potential for NH3 emission. However, management events such as fertilisation and grazing may create a high NH3 emission potential even in green plant parts. The obtained results provide input for future modelling of plant-atmosphere NH3 exchange.

scholarly journals Co-inoculation with Azospirillum brasilense in soybean cultivars subjected to water déficit

2020 ◽  
Vol 24 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Alessandra M. de L. Naoe ◽  
Joênes M. Peluzio ◽  
Leonardo J. M. Campos ◽  
Lucas K. Naoe ◽  
Roberta A. e Silva
Keyword(s):  
Grain Yield ◽  
Water Deficit ◽  
Bradyrhizobium Japonicum ◽  
Azospirillum Brasilense ◽  
Field Experiments ◽  
Crop Evapotranspiration ◽  
Plant Genotype ◽  
Soybean Cultivars ◽  
Sowing Dates ◽  
Soybean Plants

ABSTRACT This study aimed to verify the effect of co-inoculation, association between Azospirillum brasilense and Bradyrhizobium japonicum bacteria, on soybean plants subjected to water deficit at two sowing dates. Two field experiments were conducted at the Universidade Federal de Tocantins, campus of Palmas, Brazil, in 2016. The experimental design was randomized blocks in a split-split-plot arrangement with four repetitions, where the plots consisted of two irrigation depths (100 and 25% of crop evapotranspiration - ETc), the subplots was composed of two methods of inoculant application (inoculation with Bradyrhizobium japonicum and co-inoculation with Azospirillum brasilense + Bradyrhizobium japonicum) and the sub-subplots comprised two soybean cultivars (TMG 132 and ANTA 82). The cultivars responded differently to the sowing dates. Co-inoculation did not influence grain yield under full irrigation conditions (100% ETc), in neither cultivar evaluated. However, under the water deficit condition (25% ETc), the grain yield of the cultivar TMG 132 increased 77.20%, indicating that there are different responses of interaction between Azospirillum brasilense, plant genotype and sowing dates.

scholarly journals Monitoring Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) Infestation in Soybean Using Hyperspectral Remote Sensing

2020 ◽  
Author(s):  
Pedro P. S. Barros ◽  
Inana X. Schutze ◽  
Fernando H. Iost Filho ◽  
Pedro Takao Yamamoto ◽  
Peterson Fiorio ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Bemisia Tabaci ◽  
Insect Pest ◽  
Remote Sensing Data ◽  
Management Program ◽  
Hyperspectral Remote Sensing ◽  
Negative Effects ◽  
Soybean Plants ◽  
Soybean Leaves ◽  
Different Levels

Although monitoring and observing insect pest populations in the fields is essential in crop management, it is still a laborious and sometimes ineffective process. High infestation levels may diminish the photosynthetic activity of soybean plants, affecting their development and reducing the yield. An imprecise decision making in integrated pest management program may lead to an ineffective control in infested areas or the excessive use of insecticides. In order to reach a more efficient control of arthropods population it is important to evaluate the infestation in time to mitigate its negative effects on the crop and remote sensing is an important tool for monitoring. It was proposed that infested soybean areas could be identified, and the arthropods quantified from non-infested areas in a field by hyperspectral remote sensing. Thus, the goals of this study were to investigate and discriminate the reflectance characteristics of soybean non-infested and infested with Bemisia tabaci using hyperspectral remote sensing data. Therefore, samples of infested and non-infested soybean leaves were collected and transported to the laboratory to obtain the hyperspectral curves. The results obtained allowed to discriminate the different levels of infestation and to separate healthy from whitefly infested soybean leaves based on their reflectance.

Impact of elevated CO2 on carbohydrate and ureide concentrations in soybean inoculated with different strains of Bradyrhizobium japonicum

Botany ◽  
2011 ◽  
Vol 89 (7) ◽  
pp. 481-490 ◽  
Author(s):  
Annick Bertrand ◽  
Danielle Prévost ◽  
Christine Juge ◽  
François-P. Chalifour
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Feedback Inhibition ◽  
Nitrogenase Activity ◽  
Soluble Sugars ◽  
Dry Mass ◽  
Energy Reserves ◽  
Commercial Strain ◽  
Growth Chambers ◽  
Different Strains ◽  
Selection Of

Elevated CO2 increases soybean growth and photosynthesis, and the resulting additional supply of photosynthates stimulates nodule activity. To characterize its biochemical response to both CO2 and bradyrhizobial strains, soybean inoculated with three strains of Bradyrhizobium japonicum was grown in growth chambers under ambient (400 µmol·mol–1) or elevated (800 µmol·mol–1) CO2. Soluble sugars were generally more abundant in leaves and nodules under elevated CO2, while starch and pinitol were depleted, indicating that additional photosynthates were rapidly used, in particular for nodule growth (dry mass increased by 65%). Ureides (allantoin and allantoic acid) increased under elevated CO2 in leaves, while this increase was not significant in nodules. The indigenous strain 12NS14 induced the highest ureides concentration in nodules under elevated CO2 along with the highest nitrogenase activity and increase in shoot dry mass, indicating a positive-feedback stimulation: soybean mobilized energy reserves to support more nodules, and in return nodules synthesized more ureides to support plant growth. In contrast, the commercial strain 532c resulted in the highest ureide concentrations in leaves, coupled with the lowest nitrogenase activity and nodules yield, suggesting a feedback inhibition of nodule activity. Our results show that selection of B. japonicum strains better adapted to elevated CO2 could improve soybean performance.

scholarly journals Nodulation and Development of Soybean Submitted to Inoculation With Bradyrhizobium japonicum and Phosphorus Doses

2018 ◽  
Vol 10 (12) ◽  
pp. 321 ◽  
Author(s):  
Erica Chaves ◽  
Rubson da Costa Leite ◽  
Thalita Rodrigues Silva ◽  
Thayny Alves Viana ◽  
Tatiane de Sousa Cruz ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Experimental Design ◽  
Bradyrhizobium Japonicum ◽  
Phosphate Fertilizer ◽  
Dry Mass ◽  
Root Number ◽  
Phosphate Fertilization ◽  
Soybean Plants ◽  
Biological Nitrogen ◽  
Plant Dry Mass

Among the several factors that may influence nodulation and the efficiency of biological nitrogen fixation for soybean plants, nutrient availability is among the most important. This study aimed to evaluate the inoculation with Bradyrhizobium japonicum and doses of phosphorus on the development of soybean in a Vertisol, in Tocantins. The experimental design was completely randomized in a 4 &times; 2 factorial scheme, with four replications. Four doses of phosphate fertilization (0, 100, 200, and 300 kg ha-1 P2O5) were studied, combined with two inoculation treatments with Bradyrhizobium japonicum (inoculated and not inoculated). The following variables were evaluated: plant height, stem diameter, nodules per plant, dry mass of nodules, dry mass of plant, dry mass of root, number of pods and number of grains per pod. Under greenhouse conditions and soil with good availability of phosphorus, there is no influence of the doses on the inoculation with Bradyrhizobium japonicum. Soils with good availability of phosphorus have low response to the application of phosphate fertilizer.

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