scholarly journals Bioassay-guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-resistant Staphylococcus aureus

2021 ◽  
Author(s):  
Suwaiba Muhammad Ladan ◽  
Yusuf Wada ◽  
Mustapha Mohammed ◽  
Sani Ibrahim ◽  
Kabir Musa Yusuf ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Diethyl Ether ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Public Health Problem ◽  
Methicillin Resistant ◽  
Acid Methyl ◽  
Senna Alata

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.

scholarly journals Bioassay-Guided Identification of Bioactive Compounds from Senna alata L. against Methicillin-Resistant Staphylococcus aureus

2021 ◽  
Vol 1 (3) ◽  
pp. 520-536
Author(s):  
Suwaiba Ladan Muhammad ◽  
Yusuf Wada ◽  
Mustapha Mohammed ◽  
Sani Ibrahim ◽  
Kabir Yusuf Musa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Diethyl Ether ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Methicillin Resistant ◽  
Acid Methyl ◽  
Senna Alata

Senna alata (Linn) Roxb. plant is widely used to manage various infections in folkloric medicine. Methicillin-resistant Staphylococcus aureus (MRSA) infection continues to be a major global public health problem. This study aims to investigate the bioactive components of S. alata leaves active against MRSA. The leaves of S. alata were sequentially extracted and fractionated using standard methods and screened for activities against MRSA. The diethyl ether active thin layer chromatography (TLC) spot was subjected to infrared (IR) and gas chromatography-mass spectroscopic (GC-MS) studies. The aqueous extract and diethyl ether fraction of S. alata leaves elicited the highest activity against the MRSA. The GC-MS analysis of the fraction produced 15 eluates; only the sub-fraction 13 was effective. The TLC analysis of the sub-fraction 13 revealed three spots; only the second spot produced activity. The GC-MS result of the spot showed six peaks. The spectral results for peak 3 match the data from the IR study, suggestive of 9-octadecenoic acid methyl ester. Senna alata leaves possess bioactive compounds closely related to 9-octadecenoic acid methyl ester with potent antibacterial activity against MRSA.

scholarly journals Fatty acid profile, antioxidant and antibacterial effect of the ethyl acatate extract of cleistopholis patens

2021 ◽  
Vol 3 (1) ◽  
pp. 21-31
Author(s):  
Daniels A.O ◽  
Taye Temikotan
Keyword(s):  
Staphylococcus Aureus ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Profile ◽  
Fungal Infections ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Shigella Dysenteriae ◽  
Human Pathogens ◽  
Acid Methyl

Cleistopholis patens is a tropical plant that is used in the treatment of many bacterial and fungal infections. In this study, the antibacterial action against some human pathogens and the fatty acid profile of the plant were investigated. The plant was found to be active against Salmonella typhi Streptococcus pyogenes, Staphylococcus aureus while Shigella dysenteriae was resistant. The comparative antibiotic test revealed that only Staphylococcus aureus was sensitive to chloramphenicol and gentamycin. The purified extract showed lesser activity than the crude extract.  Phytochemical components include; glycosides, steroids, Phenol, tanins and saponins. Anticonstituents include tanin (2.32mg/g), phenol (2.50 mg/g), phytate (15.65 mg/g), oxalate (6.57 mg/g), saponin (9.71 mg/g) and flavonoids (6.49 mg/g). The GCMS profile of the ethyl acetate extract of the plant revealed 23 fatty acid including 9-Hexadecanoic acid, (25.11%), 6 octadecanoic acid (21.98%), n Hexadecanoic (4.62%), Cyclopentaneundecanoic acid, methyl ester (2.05%). Heptacosanoic acid, methyl ester (2.05%), Decanoic acid, methyl ester (1.74%), Oleic acid (4.38%), 16-Octadecenoic acid, methyl ester (3.55%) amongst others.

scholarly journals Phytochemical and antibacterial properties of sea cucumber (Muelleria lecanora) from Barrang Lompo Islands, Makassar South Sulawesi

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1885-1895
Author(s):  
M. Yusuf ◽  
Nur Fitriani U.A. ◽  
L. Mahyati ◽  
M. Imran
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Methyl Ester ◽  
Bioactive Compounds ◽  
Sea Cucumber ◽  
Antibacterial Properties ◽  
Acid Methyl Ester ◽  
Diffusion Method ◽  
Acid Methyl ◽  
Pharmaceutical Industries

Barrang Lompo Island Waters is home for different species of marine biota of sea cucumber (Muelleria lecanora). Many sea cucumber species have been used as health supplements because they contain bioactive compounds that are beneficial to people in Indonesia. Given this, our study was designed to investigate the phytochemical, and antibacterial properties of crude acetone, methanol and hexane extract of sea cucumber using maceration extraction methods. The sea cucumber extract was prepared and the phytochemical profile was studied by analysing Gas Chromatography-Mass Spectrometry (GC-MS). Results showed that the extracts were a complex mixture of numerous compounds; many of which were present in trace amounts antioxidants and antimicrobial; hexadecanoic acid, methyl ester, 9-octadecenoic acid (z) -, methyl ester (stearic acid methyl ester), octadecanoic acid, methyl ester, 2-[(hexadecyloxy)methyl]oxirane, cholest5-en-3-yl acetate, ergosta-14,22-dien-3-ol, acetate,(3.beta.,5.alpha.,22e), 5,8,11,14- eicosatetraenoic acid, methyl ester, (all-z) epa/omega 3, pentacosane, hexatriacontane, and 9-hexadecenoic acid, methyl ester, (Z). The extract was also evaluated for activity against three pathogenic bacterial strains (Escherichia coli, Staphylococcus aureus and Salmonella) using the disc diffusion method. The extract exhibited clear zones of inhibition against the tested bacteria. Maximum inhibitory zone concentration values were demonstrated to be: Escherichia coli = 6.84 mm , Staphylococcus aureus = 7.22 mm, and Salmonella = 7.87 mm. These results revealed the significant potential of sea cucumber as a source of antioxidants and antimicrobial agents and also highlight the necessity of further purification and characterisation of solitary bioactive compounds for their prospective applications in pharmaceutical industries, food, and nutraceutical (food functional)

scholarly journals Sandalwood, an Indian medicinal plant attenuates the microbial growth and influence up/down regulation of the metabolites

2021 ◽  
Author(s):  
Romana Parveen ◽  
Tooba Naz Shamsi ◽  
Sadaf Fatima
Keyword(s):  
Methyl Ester ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Trimethylsilyl Ester ◽  
E Coli ◽  
Acid Methyl ◽  
Soxhlet Apparatus ◽  
Antibacterial Assay

AbstractThe methanolic extract of sandalwood (SwME) was prepared by soxhlet apparatus and the antibacterial assay was performed. Further, the metabolite profiling of SwME and lysates of E. coli and E. coli grown in the presence of SwME was generated. SwME showed maximum inhibition against E. coli (MTCC 443) i.e. 82.71%, and minimal against B. subtilis (MTCC 736) i.e. 26.82%. The metabolome profiles of E. coli and SwME were generated using gas chromatography-mass spectrometry (GC-MS) technique. Comparative studies were done to understand to what extent metabolite modifications differ between SwME, E. coli lysate and the E. coli strain grown in presence of extract. Result revealed 23 peaks with major compounds present in E. coli were 9-Octadecenoic Acid (Z)-, Methyl Ester (26.85%), Hexadecanoic Acid, methyl ester (20.5%) and Hexadecanoic acid, trimethylsilyl ester (15.79%). When E. coli was grown in the presence of SwME, GC-MS analysis showed 25 peaks with major compounds such as 9-Octadecenoic Acid, Methyl Ester (21.97%), Hexadecanoic Acid, Methyl Ester (17.03%), and Hexadecanoic Acid, Trimethylsilyl Ester (14.96%). Correlating the metabolic profiles with the changes occurring is essential to progression their comprehension and in the development of new approaches to identify the metabolomics regulation in E. coli in response to SwME.

scholarly journals Phytochemical Profiling and Larval Control of Erythrina variegata Methanol Fraction against Malarial and Filarial Vector

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Mathalaimuthu Baranitharan ◽  
Barbara Sawicka ◽  
Jayapal Gokulakrishnan
Keyword(s):  
Methyl Ester ◽  
Methanol Extract ◽  
Mosquito Control ◽  
Chemical Constituents ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Methanol Fraction ◽  
Mass Spectral ◽  
Erythrina Variegata ◽  
Acid Methyl

Erythrina variegata (E. variegata) bioactive chemical has been the potential to be utilized as a good, eco-friendly approach for the control of mosquito population. In the present investigation, methanol extract using insecticidal compounds isolated against mosquito larvae kill assay was carried out. Secondary metabolism was characterized by thin layer chromatography, column chromatography, Fourier transform-infrared spectroscopy, gas chromatography-mass spectral, and identification of compound. Mosquito immature third instar larval, Anopheles stephensi, and Culex quinquefasciatus have been exposed to different concentrations of 50-250 µg/ml. Totally, larvae were death rate 98.2% (significant value 0.001b) from methanol extract and it is significant toxicity against larvae of An. stephensi and Cx. quinquefasciatus with LC50/LC99 values were 157.69/339.55 µg/ml and 137.67/297.33 µg/ml, respectively. FT-IR analysis in the functional groups such as alcohol, amines, amides, alkenes, 1⁎ amines, aromatic amines, aliphatic amines, 1⁎,2⁎ amines, and alkyl halides searched the identity of secondary metabolites, which may act as 12-Octadecenoic acid, methyl ester compound and clearly indicates being phytochemical. Chemical constituents of twenty-five compounds were identified in the methanol extract. The major components were 12-Octadecenoic acid and methyl ester (37.31%). Compound molecules consist of carbon 19 atoms (gray), hydrogen 36 atoms (greenish blue), and oxygen 2 atoms (red), indicated by the different colors. The results were obtained suggesting that, in addition to their pharmaceutical and medicine sources, 12-Octadecenoic acid, methyl ester compound can also serve as a natural mosquito control.

Fatty Acid Composition of Seeds of Satureja thymbra and S. cuneifolia

2003 ◽  
Vol 58 (7-8) ◽  
pp. 502-504 ◽  
Author(s):  
Ahmet C. Gören ◽  
Gökhan Bilsel ◽  
Mehmet Altun ◽  
Fatih Satıl
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Methyl Esters ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Octadecatrienoic Acid ◽  
Octadecanoic Acid ◽  
Hexadecanoic Acid ◽  
Acid Methyl ◽  
Main Components

Abstract The chemical composition of fatty acid methyl esters (FAMEs) from seeds of S. thymbra and S. cuneifolia were analyzed by GC/MS. 7 FAMEs were identified from the seeds of S. thymbra mainly as 9-octadecenoic acid methyl ester (43.9%), hexadecanoic acid methyl ester (11.4%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (30.2%), and octadecanoic acid methyl ester (14.1%), while from the seed of S. cuneifolia 10 FAMEs were obtained with the main components, similar to S. thymbra. These were identified as 9-octadecenoic acid methyl ester (10.1%), hexadecanoic acid methyl ester (methyl palmitate, 34.6%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (6.3%) and octadecanoic acid methyl ester (1.8%).

Analysis of Main Flavor Substances of Low- and Full-Fat Cheese Fermented by Lactobacillus acidophilus during Ripening Process

2011 ◽  
Vol 236-238 ◽  
pp. 2395-2398
Author(s):  
Rui Xiang Zhao ◽  
Cui Qing Hu ◽  
Sheng Yang Niu ◽  
Hua Di Sun ◽  
Xiao Hong Ge
Keyword(s):  
Methyl Ester ◽  
Lactobacillus Acidophilus ◽  
Mass Fraction ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Streptococcus Thermophilus ◽  
Relative Mass ◽  
Low Fat ◽  
Acid Methyl ◽  
Low Fat Cheese

Low- and full-fat cheeses were fermented by Lactobacillus acidophilus and adjunct cultures Streptococcus thermophilus. After ripening for 90d at 4°C, the main flavor substances were determined by gas chromatograph. The results showed that more than 28 main flavor components were founded in full-fat cheese, and at least 19 were founded in low-fat cheese. In low-fat cheese, there was a higher content of 9-(Z)- octadecenoic acid and 10-hydroxy-octanoic acid methyl ester, which their relative mass fraction were 19.20% and 18.79% respectively. In full-fat cheese, there was a higher content of hexadecanoic acid methyl ester and 9-Octadecenoic acid(Z)-methyl ester, which their relative mass fraction were 24.27%and 23.37% respectively.

scholarly journals Isoprenoids and Fatty Acids Derivatives from the Chloroform Fraction of the Antimycobacterial Methanol Extract Ximeniaamericana Lam. (Olacaceae) Stem Bark

2019 ◽  
Vol 6 (2) ◽  
pp. 101-106
Author(s):  
Ozadheoghene Eriarie Afieroho ◽  
L. Lawson ◽  
Nnamdi Emenyonu
Keyword(s):  
Fatty Acids ◽  
Mycobacterium Tuberculosis ◽  
Methyl Ester ◽  
Palmitic Acid ◽  
Methanol Extract ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Stem Bark ◽  
Clinical Strains ◽  
Acid Methyl

This study investigated the triterpenoids and fatty acid derivatives, and the in vitro growth inhibitory effect against clinical strains of Mycobacteria tuberculosis of the stem bark of Ximenia Americanaa plant widely used in ethno-medicine for the treatment of bacterial and skin infections, poison, post-partum hemorrhage, anaemia, and dysentery. The macerated methanol extract (XAM) of the stem bark was evaluated for anti-tuberculosis activity using the Lowensten Jensen method against de-contaminated clinical strains of Mycobacterium tuberculosis. The XAM was fractionated by open column chromatography on a normal phase silica gel column with a 25 % stepwise gradient of chloroform-methanol as mobile phase. The constituents of the non-polar column fractions eluted with 100% chloroform were characterized using Gas Chromatography-Mass spectroscopic (GC-MS) techniques and by comparison with reference NIST library compound. The XAM (5 mg/mL) inhibited the growth of the Mycobacterium tuberculosis. GC-MS analysis of the non-polar column fractions afforded Two lupane-type triterpenoids: Lup-20-(29)-en-3-one (15) and lupeol (16), three phytosteroids: campesterol (11), stigmasterol (12) and gamma-sitosterol (14), one fridelane-type triterpenoid: Friedelan-3-one (8), one oleanane-type triterpenoid: 12-oleanen-3-one (13), and the fatty acids: Palmitic acid methyl ester (1), Palmitic acid (2), 11-octadecenoic acid methyl ester (3), Octadecanoic acid methyl ester (4), Cis-13-Octadecenoic acid (5), 10,13-octadecadiynoic acid methyl ester (6), Docosanoic acid (7), Tetracosanoic acid (9), and Hexacosanoic acid methyl ester (10). The presence of these bioactive triterpenoids and fatty acids could offer an explanation for the ethno-medicinal uses of this plant. Further work is on-going to isolate in pure form, and characterized the bioactive constituents in the XAM with the view of discovery lead compounds for the treatment of tuberculosis and associated opportunistic bacterial infections.  

scholarly journals Chemosensory response of the Phaseolus vulgaris L. (Fabaceae) weevil (Bruchus chinensis Linnaeus, 1758 (Coleoptera: Bruchidae)) to Dioscorea sansibarensis Pax. (Dioscoreaceae) bulbil essential oil

2019 ◽  
Vol 88 (2) ◽  
pp. 183-195 ◽  
Author(s):  
Godfrey Omare Mauti ◽  
Peter F. Kasigwa ◽  
Joan J.E. Munissi ◽  
Justus M. Onguso
Keyword(s):  
Methyl Ester ◽  
Phaseolus Vulgaris ◽  
Octadecenoic Acid ◽  
Acid Methyl Ester ◽  
Design Experiment ◽  
Hexadecanoic Acid ◽  
Botanical Pesticide ◽  
Acid Methyl ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract Dioscorea sansibarensis bulbils are well known for their toxicity and medicinal applications. In this study, the attraction and mortality of the Phaseolus vulgaris weevil (Bruchus chinensis) caused by Dioscorea sansibarensis bulbil oil was examined in a Completely Randomized Design experiment using a Y-tube olfactometer on a laboratory bench. Statistical analysis at P < 0.05 showed 10 µL had an attraction of 42.67%. In the case of mortality, the results showed that Phaseolus vulgaris seeds treated with 40 µL of the bulbil oil had a harmonic mean of 55.8% mortality of the weevil population, which was higher than with 10 µL of Actellic 50 EC. GC-MS analysis identified 17 compounds in the bulbil oil, the main constituents of which were 2- pentanone, 4-hydroxy-4-methyl- (17.8%), phenol, 4-ethyl- (11.41%), hexadecanoic acid, methyl ester (11.05%), phenol (7.35%), 9-octadecenoic acid, (E)- (7.2%) and phenol, 4-ethyl-2-methoxy- (7.14%). Attraction by bulbil oil may have been due to the presence of phenolic compounds, and mortality may have been due to hexadecanoic acid, methyl ester and 9-octadecenoic acid, (E)-. Attractants are used for the surveillance and detection of an infestation in stored products. The added advantage of the bulbil oil to cause mortality makes Dioscorea sansibarensis bulbil oil useful in the formulation of a botanical pesticide in stored pest management.

scholarly journals GC-MS ANALYSIS OF BIO-ACTIVE COMPOUNDS FROM THE ETHANOLIC EXTRACT OF BALIOSPERMUM MONTANUM (WILD.) MUELL. ARG.

2018 ◽  
Vol 5 (1) ◽  
pp. 42-45
Author(s):  
Reshma K ◽  
Baluprakash T ◽  
Abdul Kaffoor H ◽  
Venkatachalapathi A ◽  
Arumugasamy K
Keyword(s):  
Methyl Ester ◽  
Medicinal Plant ◽  
Bioactive Compounds ◽  
Mass Spectra ◽  
Octadecadienoic Acid ◽  
Acid Methyl Ester ◽  
Ethanolic Extract ◽  
Acid Methyl ◽  
Pharmacological Studies ◽  
Phytochemical Compounds

Baliospermum montanum (Wild.) Muell. Arg., (Euphorbiaceae) is a well known medicinal plant which is used in treatment of various diseases. The present study was focussed on the separation and investigation of the phytochemical compounds from ethanolic extract by GC-MS technique. The mass spectra of thecompounds found in the extract were matched with the National Institute of Standards and Technology (NIST) library. The ethanolic extract revealed the presence of 30 bioactive compounds. The major and minor phytochemical compounds are 2,15-Dithia[3](9,10)anthracenol[3](2,6)pyridinophane,1,2,3,4-tetrahydro showed the highest peak 9.32% followed by phytol-9.08%, Neophytadiene-7.38%, 8,11-Octadecadienoic acid, methyl ester (CAS)-7.06%, 4-ethyl-6-[2-(methoxycarb onyl)ethenyl]-7-[2-(methoxycarbonyl)ethyl]1,3,5,8-tetramethyl-2-vinylporphyrin-6.72%, 2-pentafluorophenylpropanal-6.43%,4,4'-Isopropylidene-bis-(2-cyclo hexyl phenol)-5.91% etc.. Further pharmacological studies are needed to find out the medicinal aspect of these compounds.

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