scholarly journals ANTIOXIDANT ACTIVITY IN COMBINATION EXTRACT OF Acorus calamus L. (DLINGU) AND Allium sativum (GARLIC)

2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Pramita Laksitarahmi Isrianto ◽  
Putu Oky Ari Tania
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Experimental Research ◽  
Allium Sativum ◽  
Acorus Calamus ◽  
Test Results ◽  
Phytochemical Screening ◽  
Natural Materials ◽  
Activity Test ◽  
Ic50 Values

One of the efforts to optimize the utilization of natural materials that is used them as medicinal plants, including Dlingu (Acorus calamus L.) and Garlic (Allium sativum) which have the potential as antioxidants. This study aims to determine the effectiveness of a combination extract of Acorus calamus L. (Dlingu) and Allium sativum (Garlic) on antioxidant activity. This research is an experimental research which is the test of antioxidant content and antioxidant activity test with 5 series of concentrations and 4 times of repetition. The research materials, namely dlingu stem and single clove garlic tubers were extracted, phytochemical screening was carried out and then antiradical free IC50 was determined. Based on the test results of the chemical extract of dligu and single clove garlic, the highest content was alkaloid 5.16%, tannin 4.05%, saponin 3.01%, and flavonoid 2.18%. The test results of the chemical antioxidant activity of extracts dligu and single clove garlic contain IC50 values of 17.062 ppm, including a very strong class of antioxidants.Keywords: antioxidant, Dlingu, Single Clove (Male) Garlic.

scholarly journals UJI FITOKIMIA DAN PENENTUAN Inhibition Concentration 50% PADA BEBERAPA TUMBUHAN OBAT DI PULAU TIDORE

2014 ◽  
Vol 14 (2) ◽  
pp. 95
Author(s):  
Wiwin Abdullah ◽  
Max Revolta J. Runtuwene ◽  
Vanda Selvana Kamu
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Seed Extract ◽  
Test Results ◽  
Active Compounds ◽  
Inhibition Concentration ◽  
Dpph Method ◽  
Ic50 Values

ABSTRAK Penelitian ini dilakukan untuk mengetahui senyawa aktif yang terkandung melalui pengujian fitokimia dan aktivitas antioksidan pada tumbuhan obat di pulau Tidore. Uji aktivitas antioksidan menggunakan metode DPPH dilanjutkan dengan perhitungan Inhibition Concentration 50% (IC50). Hasil yang diperoleh adalah biji buah mojoi terkandung senyawa alkaloid dan saponin, buah coro terkandung alkaloid, flavonoid, dan saponin, pada daun ofo terkandung  alkaloid, tanin, flavonoid, steroid, dan saponin dan pada rimpang kuso mafola terkandung alkaloid, tanin, flavonoid, steroid, dan saponin. Nilai IC50 sebagai berikut biji rimpang kuso mafola 37,30 ppm, buah coro 250,17 ppm, daun ofo 976,10 ppm  dan buah mojoi 1001, 07 ppm. Kata kunci :Tumbuhan  obat, DPPH, antioksidan dan uji fitokimia. PHYTOCHEMICALS TEST AND DETERMINATION Inhibition Concentration 50% ON SOME MEDICINAL PLANTS IN THE TIDORE ISLAND ABSTRACT This study was conducted to determine the active compounds contained in an assessment of phytochemical and antioxidant activity in the medicinal plants of Tidore island. The test antioxidant activity was used DPPH method. In the test results to the phytochemical , that mojoi fruit seeds contained alkaloids and saponins, fruit coro (alkaloids, flavonoids, and saponins), ofo leaves (alkaloids, tannins, flavonoids, steroids, and saponins) and ethanol extract of rhizome kusomafola (alkaloids, tannins, flavonoids, steroids, and saponins. In  calculation of IC50 values ​​for rhizome kusomafola 37.30 ppm, 250.17 ppm coro fruit, 976.10 ppm ofo leaf extract, and fruit seed extract mojoi 1001.07 ppm. Keywords: Medicinal plants, DPPH, antioxidant and phytochemical test.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN BENALU KOPI (Loranthus Ferrugineus Roxb.) DENGAN METODE DPPH (1,1 – Difenil -2- Pikrilhidrazil)

2019 ◽  
Vol 6 (2) ◽  
pp. 192
Author(s):  
Muammar Yulian ◽  
Safrijal Safrijal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
X 24

The study about antioxidant activity test of coffee parasite leaves (Loranthus ferrugineus Roxb.) by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method has been done. The aim of this study was to determine the content and activity of secondary metabolites, flavonoids and antioxidant, which found in the coffee parasite leaves. Dry powder of parasite coffee leaves (Loranthus ferrugineus Roxb.) as much as 0.5 kg were macerated by 2 L of ethanol solvent at room temperature for 4 x 24 hours, then mixed and filtered. Ethanol filtrate was evaporated at 30-40°C by using a rotary evaporator to obtain the crude extract of coffee parasite leaves. The results of the phytochemical screening showed positively that the extract was containing alkaloid, flavonoids, saponins, tannins and steroid compounds.The results of the antioxidant activity test by using DPPH method was obtained that the amount of antioxidant activity of the samples of ethanol extract had a very strong antioxidant activity against radical DPPH 0.05 mM, with IC50 values was obtained 6.063 ppm. Whereas, for comparison of ascorbic acid was about 3.127 ppm.

scholarly journals Skrining Fitokimia dan Uji Aktivitas Antioksidan dari Daun Nasi (Phrynium capitatum) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil)

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (1) ◽  
pp. 11 ◽  
Author(s):  
Dwi Putra Wijaya ◽  
Jessy E. Paendong ◽  
Jemmy Abidjulu
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dpph Solution

Telah dilakukan penelitian tentang skrining fitokimia dan uji aktivitas antioksidan dari daun nasi (Phrynium capitatum) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Skrining fitokimia meliputi pengujian alkaloid, flavonoid, saponin, steroid, triterpenoid dan fenolik. Uji aktivitas antioksidan dilakukan dengan penambahan 2 mL larutan DPPH dengan 0,5 mL larutan ekstrak dengan konsentrasi masing-masing 1000, 750, 500 dan 250 µg/mL. Data yang diproleh kemudian digunakan untuk menghitung nilai IC50 dari ekstrak etanol daun nasi. Hasil pengujian menunjukan bahwa ekstrak etanol daun nasi mengandung senyawa alkaloid, flavonoid, saponin, steroid dan fenolik. Ekstrak etanol daun nasi memiliki aktivitas antioksidan trrtinggi pada konsentrasi 1000 µg/mL sebesar 65,77 % dan semakin menurun aktivitasnya dengan berkurangnya konsentrasi ekstrak, konsentrasi 750, 500 dan 250 µg/mL masing-masing sebesar 53,96 %, 43,8 % dan 19,58 % dengan nilai IC50 pada konsentrasi 701,78 µg/mL.A research on phytochemicals screening and antioxidant activity test of daun nasi (Phrynium capitatum) using DPPH (1,1-diphenyl-2-picrylhydrazyl) method had been conducted. Test for alkaloids, flavonoids, saponins, steroids, triterpenoids and phenolic were included in phytochemical screening. Antioxidant activity was tested through the addition of 2 mL DPPH solution into 0.5 mL of the extract having a concentration of 1000, 750, 500 and 250 µg/mL respectively. The data obtained was used to calculate the IC50 value for the ethanol extract of daun nasi. The results showed that the ethanol extract of daun nasi contains alkaloids, flavonoids, saponins, steroids and phenolic. Ethanol extract of daun nasi has the highest antioxidant activity of 65.77% at concentration of 1000 µg/mL. Its activity decreased with decreasing concentration of the extract, which were 53.96 %, 43.8 % and 19.58 % at concentration of 750, 500 and 250 µg/mL respectively. IC50 values was found at a concentration of 701.78 µg/mL.

scholarly journals Antioxidant Activity of Red and Purple Rosella Flower Petals Extract (Hibiscus sabdariffa L.)

2021 ◽  
pp. 186-192
Author(s):  
Mareetha Zahra Shafirany ◽  
Iin Indawati ◽  
Lela Sulastri ◽  
Asman Sadino ◽  
Anggun Hari Kusumawati ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Dpph Radical Scavenging Activity ◽  
Activity Test ◽  
Ic50 Values ◽  
Antioxidant Agent ◽  
Dpph Radical Scavenging

Aims: This study aims to test the antioxidant activity of red and purple H. sabdariffa flower petals extract and conduct qualitative phytochemical screening. Study Design: Antioxidant potential of red and purple H. sabdariffa flower petal extract analyzed by spectrometric assays. Place and Duration of Study: This study was carried out at School of Pharmacy Muhammadiyah Cirebon, Cirebon, West Java, Indonesia from the year of 2020 to 2021. Methodology: Red and purple H. sabdariffa petals extracted with 70% ethanol. The extract was then examined for its antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method, after the qualitative phytochemical screening. Results: In this study, the concentration of red and purple H. sabdariffa petals extract dependently demonstrated the ability to scavenge DPPH. In the DPPH radical scavenging activity test, the red and purple H. sabdariffa petasl extracts designating IC50 values of 63.77 and 37.19 µg/ml and fall into the category of strong and very strong antioxidant activity. Meanwhile, phytochemical screening tests showed the existence of flavonoids and polyphenols in the extract of red and purple H. sabdariffa petals. Conclusion: This study shows that the red and purple H. sabdariffa petals extract has potential as a promising natural antioxidant agent for the treatment of oxidative stress.

scholarly journals AKTIVITAS ANTIOKSIDAN EKSTRAK AIR SIMPLISIA DAUN SEMBUNG (Blumea balsamifera)

2017 ◽  
Vol 3 (2) ◽  
pp. 129
Author(s):  
Mamay Maslahat ◽  
Febi Nurilmala ◽  
Lipi Harpeni
Keyword(s):  
Antioxidant Activity ◽  
Herbal Medicine ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Leaf Water ◽  
Test Results ◽  
Water Extracts ◽  
Dpph Method ◽  
Ic50 Values

Antioxidant Activity of Aquous Extract of Sembung Leaf (Blumea balsamifera)             Simplicia medicinal plants are more commonly known by the term of herbal medicine in the form boiling and steeping. Sembung leaf water extracts content of secondary metabolites has potential natural antioxidant. An antioxidant is a substance that can prevent or slow down the deterioration of a substance. This research aimed to test the potential of antioxidant in water extracts of leaves sembung (Blumea balsamifera) in the form of boiling and steeping using DPPH method through determination of IC50 values. Best antioxidant test results found in extracts of steeping the leaves with IC50 values of 155,65 ml/l while the extract boiling sembung with IC50 values indicated by 293,80 ml/l, antioxidant standards testing used was quercetin.Keywords : antioxidant, aquous  extract, Blumea balsamifera, DPPH ABSTRAK         Simplisia tanaman obat yang lebih dikenal dengan istilah jamu sering diaplikasikan dalam bentuk godogan dan seduhan. Ekstrak air daun sembung memiliki kandungan metabolit sekunder yang berpotensi sebagai antioksidan alami. Antioksidan merupakan substansi yang dapat mencegah atau memperlambat kerusakan suatu zat. Penelitian ini bertujuan menguji potensi antioksidan ekstrak air daun sembung (Blumea balsamifera) dalam bentuk seduhan dan godogan dengan metode DPPH melalui penentuan nilai IC50.  Hasil uji antioksidan terbaik terdapat pada ekstrak seduhan daun sembung dengan nilai IC50 sebesar 155,65 ml/l sedangkan ekstrak godogan sembung menunjukan nilai IC50 sebesar 293,80 ml/l,  sebagai standar pengujian antioksidan digunakan kuersetin.Kata kunci : Antioxidan, extract air, Blumea balsamifera, DPPH

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Phytochemical screening and antioxidant activity of some medicinal plants’ crude juices

2020 ◽  
Vol 28 ◽  
pp. e00536
Author(s):  
Radwan S. Farag ◽  
Mohamed S. Abdel-Latif ◽  
Hanaa H. Abd El Baky ◽  
Layla S. Tawfeek
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Phytochemical Screening

scholarly journals THE ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT, FRACTIONS OF WATER, ETHYL ACETATE AND n-HEXANE FROM MISTLETOE TEA (SCURRULA ATROPURPUREABL. DANS)

2017 ◽  
Vol 10 (2) ◽  
pp. 343 ◽  
Author(s):  
Resmi Mustarichie
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Values

Objective: The aim of this study is to investigate antioxidant activity and phytochemical screening of ethanol extract, fractions of water, ethyl acetate, and n-hexane from mistletoe tea (Scurrula atropurpurea Bl. Dans).Methods: Simplicia extracted using soxhlet equipment with 96% ethanol. Fractionation was conducted using liquid-liquid extraction using a solvent of water, ethyl acetate and n-hexane. Screening of phytochemical and antioxidant activity was performed against these fractions. Antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl method using ultraviolet-visible spectrophotometry with ascorbic acid as standard. Phytochemical screening was conducted based on the method of Farnsworth.Results: The IC50 values of ethanol extract, water fraction, fraction of ethyl acetate, and n-hexane fraction were 21.92 ppm, 89.57 ppm, 14.08 ppm, and 162.09 ppm, respectively, whereas for ascorbic acid was 4.41 ppm. The ethanol extract and ethyl acetate fraction contained compounds were the same group, polyphenolic, tannins, flavonoids, monoterpenoid, steroids, triterpenoids, and quinones. Fraction of water contained compounds such as polyphenolic group, flavonoids, monoterpenoids, sesquiterpenoids, steroids, and triterpenoids. n-hexane fraction compounds contained steroids and triterpenoids.Conclusion: The ethanol extract, water fraction, ethyl acetate fraction, and n-hexane fraction showed antioxidant activities. The ethanol extract, fractions of water, and ethyl acetate fraction contained flavonoids and polyphenolic potential as antioxidants.Keywords: Mistletoe tea, Scurrula atropurpurea, Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ascorbic acid.

scholarly journals Phytochemicals, Antioxidant and Antifungal Properties of Acorus calamus, Curcuma mangga, and Allium sativum

2017 ◽  
Vol 3 (6) ◽  
pp. 93
Author(s):  
Bayyinatul Muchtaromah ◽  
Mujahidin Ahmad ◽  
Emy Koestanti S ◽  
Yuni Ma’rifatul A ◽  
Velayati Labone A
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Allium Sativum ◽  
Inhibitory Concentration ◽  
Acorus Calamus ◽  
Test Results ◽  
Inhibitory Zone ◽  
Antifungal Properties ◽  
Key Word ◽  
Mic Value

The purpose of this study to determine the content of phytochemicals, antioxidant and antifungal properties of the combination of Acorus calamus, Curcuma mangga, and Allium sativum. This research was descriptive qualitative, extractions were done by maceration method with ethanol with 3 different combinations (C1, C2 and C3). Phytochemical test reagent included 4 kinds of test, namely: alkaloids, flavonoids, triterpenoids, saponins and tannins. As for the antioxidant test, the method used was DPPH. The concentration used at 25, 50, 100, 200, and 400 ppm. As for the antifungal test conducted on Candida albicans with Kirby-Bauer disc methods with a concentration of 100%, followed by the MIC and MBC test with a concentration of 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78% and 0.39%. Phytochemical test results indicated the presence of the alkaloids, flavonoids and triterpenoids compounds in 3 different combinations (C1, C2 and C3). The highest antioxidant levels founded in C1 (61.75) followed by C3 (47.94) and the lowest levels founded in C2 (42.76). The antifungal test showed the inhibitory zone against C.  albicans. The highest inhibitory zone was found in C1 at 5.44 ± 1.78 mm (medium category), followed by C2 at  4.08 ± 0.86 mm (medium category), and C3 at 3.05 ± 0.23 mm (medium category). As for the minimum inhibitory concentration (MIC) value got on the concentration of 0:39% and minimum fungisidal concentration (MFC) values were at a concentration of 0.78%.    Key word: Acorus calamu, Allium sativum, Curcuma manga, Antioxidant, antifungal, Candida albicans

scholarly journals Antioxidant Activity Test of Effervescent Granules Morinda citrifolia L Leaf Extract with DPPH Free Radical Absorption Method

2021 ◽  
Vol 5 (4) ◽  
pp. 365-368
Author(s):  
Muthmainah Tuldjanah ◽  
Niluh Puspita Dewi ◽  
Dian Rahmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Natural Antioxidants ◽  
Morinda Citrifolia ◽  
Absorption Method ◽  
Immersion Method ◽  
Activity Test ◽  
Ic50 Values

Morinda citrifolia. L leaf is a plant that has the potential as an antioxidant due to the presence of compounds in the form of flavonoids and other phenolic compounds that can function as natural antioxidants. In connection with this, research was carried out to Morinda citrifolia. L formulate leaf extract in the form of effervescent granules. Morinda citrifolia leaf extract was macerated with ethanol 96% as solvent. Phytochemical screening showsthat the extract contains flavonoids, tannins, saponins and alkaloids. The extract was formulated with various concentrations of F1 (20%), F2 (25%), F3 (4%). Determination of antioxidant activity using the DPPH free radical immersion method. The results showed that the concentration in the granules influenced antioxidant activity with IC50 values of 109.05 ppm, 101.33 ppm and 73.28 ppm, respectively

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