PHYTOCHEMICAL ANALYSIS OF BIOACTIVE COMPOUNDS OF SYZYGIUM CUMINI (L.) SKEELS.

2017 ◽  
pp. 1361-1379
Author(s):  
Mariappan Senthilkumar
Keyword(s):  
Bioactive Compounds ◽  
Phytochemical Analysis ◽  
Syzygium Cumini

scholarly journals Phytochemical Screening of leaf extract of Syzygium Cumini L. by HRLC-MS Spectra Method

2018 ◽  
pp. 33-37
Author(s):  
Satpute S. B. ◽  
Vanmare D.J.
Keyword(s):  
Bioactive Compounds ◽  
Leaf Extract ◽  
Rapid Identification ◽  
Drug Formulation ◽  
Charge Ratio ◽  
Phytochemical Analysis ◽  
Molecular Formula ◽  
Syzygium Cumini ◽  
Phytochemical Screening ◽  
Phytochemical Study

By using HRLC-MS spectra method, Phytochemical screening of leaves of Syzygium cumini L. were carried out. It is highly sensitive and used for rapid identification of bioactive compounds. These mass spectra are fingerprint of that compound which can be identified from the data library. It may be the first report of its kind to analyze the bioactive compounds of Syzygium cumini L. The phytochemical study indicates the presence of important bioactive compounds which can be used to treat many diseases in medical field. Phytochemical analysis of methanolic leaf extract of Syzygium cumini L. spectrum profile shows total 80 compounds, out of that 19 major compounds were confirmed on the basis of their retention time, mass, molecular formula and mass per charge ratio (m/z). The findings of this study suggested that, Syzygium cumini L. is a source of important bioactive compounds. It may be contributing natural antimicrobial, antioxident, anti-inflammentary activity and further investigations which may lead to the development of drug formulation.

Untargeted metabolite profiling and phytochemical analysis based on RP‐HPLC‐DAD‐QTOF‐MS and MS/MS for discovering new bioactive compounds in Rumex algeriensis flowers and stems

2020 ◽  
Vol 31 (5) ◽  
pp. 616-635
Author(s):  
Sonda Ammar ◽  
Jouda Abidi ◽  
Simon Vlad Luca ◽  
Mahieddine Boumendjel ◽  
Krystyna Skalicka‐Woźniak ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Metabolite Profiling ◽  
Phytochemical Analysis ◽  
Rp Hplc ◽  
Untargeted Metabolite Profiling

Preliminary phytochemical analysis and evaluation of antioxidant, cytotoxic and inhibition of lipopolysaccaride - induced NOS (iNOS) expression in BALB/c mice liver by Ziziphus oenoplia Mill. fruit

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Ramaraj Thirugnanasampandan ◽  
Gunasekar Ramya ◽  
Gunasekaran Bhuvaneswari ◽  
Srinivasan Aravindh ◽  
Suresh Vaishnavi ◽  
...  
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Tamil Nadu ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Ms Analysis ◽  
Wide Range ◽  
Mice Liver

AbstractBackground:Mill. is an ethnomedicinal plant and its fruit has been traditionally used by Puliar tribes of Anamalai Hills, Tamil Nadu, India to treat various ailments. Phytochemical analysis, antioxidant, cytotoxic and inducible nitric oxide synthase (Methods:To explore bioactive compounds present in the ripened fruits, high-performance thin-layer chromatography (HPTLC) and gas chromatography/mass spectrometry (GC–MS) analysis were done. Free radical scavenging, hepatoprotective, inhibition ofResults:Total flavonoid content of ZOFwas estimated as 69 µg/mg catechin equivalent. HPTLC densitogram confirmed the presence of quercetin and GC–MS analysis showed a total of 16 compounds of 87.66 % with quinic acid as a major compound which accounted for 22.29 %. Free radical-scavenging activity of ethanolic fruit extract was ranged from 160.12 to 650.23 µg/mL. An amount of 1.5 µg lipopolysaccharide (LPS)- induced severe inflammation in BALB/c mice liver, followed by treatment with ethanolic fruit extract of 100 µg concentration, exhibited significant hepatoprotection and reverse transcriptase polymerase (RT-PCR) analysis showed downregulation ofConclusions:Findings of the present study prove that ZOF is a rich source of bioactive compounds with a wide range of pharmacological activities. Hence, consumption of this wild edible fruit will be a cost-effective and easily available natural nutritional source for health protection.

scholarly journals Lilium Philadelphicum Flower as a Novel Source of Antimicrobial Agents: A Study of Bioactivity, Phytochemical Analysis and Partial Identification of Antimicrobial Metabolites

2021 ◽  
Author(s):  
Shefali Singh ◽  
Vineeta Singh ◽  
Alaa Alhazami ◽  
B.N. Mishra ◽  
Shafiul Haque ◽  
...  
Keyword(s):  
Cell Viability ◽  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Achromobacter Xylosoxidans ◽  
Phytochemical Analysis ◽  
Antimicrobial Potential ◽  
Compound Concentration

The members of the Liliaceae family have been regarded as an excellent source of biologically active compounds. However, the work on antimicrobial potential and characterization of the bioactive fractions of Lilium philadelphicum flower is limited and needs to be explored. The present study reports the antimicrobial potential, anti-inflammatory and anticancer potential of the bioactive fraction extracted from the flower of L. philadelphicum (Red Lily) and characterization of these bioactive compounds. The antimicrobial activity was tested against nine different Gram-positive and Gram-negative bacterial strains. The minimum inhibitory concentration (MIC) values of methanolic extract of L. philadelphicum flower against Acinetobacter bouvetii, Achromobacter xylosoxidans, Bacillus subtilis MTCC 121, Candida albicans MTCC 183, Klebsiella pneumoniae MTCC 3384, and Salmonella typhi MTCC 537 were 25, 50, 12.5, 50, 100 and 50 μg mL-1, respectively. The phytochemical analysis of the extract reveals the presence of phenols, flavonoids, tannins, terpenoids, glycosides, coumarins, and quinones. The cytotoxicity of the partially purified compound against the HepG2 cell line in MTT assay demonstrates up to 90% cell viability with a bioactive compound concentration of 50 μg/ml. However, with the increase in bioactive compound concentration up to 1000 μg/ml results into nearly 80% cell viability, just a minor decline in cell viability suggests the importance of bioactive compounds for suitable therapeutic applications. Spectroscopic studies of the bioactive compound by UV-Visible spectroscopy, FT-Infra Red spectroscopy, Gas Chromatography-Mass Spectrometry (GCMS) as well as its phytochemical analysis suggests the presence of terpenoids moiety, responsible for the antimicrobial property of L. philadelphicum flower.

scholarly journals Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 90-94
Author(s):  
P Sasikala ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
M Padma ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fine Powder ◽  
Ethanol Extract ◽  
Taraxacum Officinale ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Biological Compounds ◽  
Phytochemical Compounds ◽  
Medicinal Properties ◽  
Phytochemical Constituents

Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds. Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis. Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial  and antidiabetic compounds. Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various  medicinal  properties to cure dangerous diseases and disorders. Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds.

A Tropical Lichen, Dirinaria consimilis Selectively Induces Apoptosis in MCF-7 Cells through the Regulation of p53 and Caspase-Cascade Pathway

2020 ◽  
Vol 20 (10) ◽  
pp. 1173-1187
Author(s):  
Anil K. Shendge ◽  
Sourav Panja ◽  
Tapasree Basu ◽  
Nripendranath Mandal
Keyword(s):  
Western Blotting ◽  
Bioactive Compounds ◽  
Flow Cytometric Analysis ◽  
Annexin V ◽  
Phytochemical Analysis ◽  
Crude Mortality Rate ◽  
Crude Mortality ◽  
Caspase Cascade ◽  
Mcf 7

Background: Breast cancer is the most leading cause of death, with 49.9% of crude incidence rate and 12.9% of crude mortality rate. Natural resources have been extensively used throughout history for better and safer treatment against various diseases. Objectives: The present study was aimed to investigate the antioxidant and anticancer potential of a tropical lichen Dirinaria consimilis (DCME) and its phytochemical analysis. Methods: The DCME was preliminarily evaluated for ROS, and RNS scavenging potential. Furthermore, DCME was evaluated for in vitro anticancer activity through cell proliferation assay, cell cycle analysis, annexin V/PI staining, morphological analysis, and western blotting study. Finally, the HPLC and LC-MS analyses were done to identify probable bioactive compounds. Results: The in vitro antioxidant studies showed promising ROS, and RNS scavenging potential of DCME. Moreover, the in vitro antiproliferative study bared the cytotoxic nature of DCME towards MCF-7 (IC50 - 98.58 ± 6.82μg/mL) and non-toxic towards WI-38 (IC50 - 685.85 ± 19.51μg/mL). Furthermore, the flow-cytometric analysis revealed the increase in sub G1 population as well as early apoptotic populations dose-dependently. The results from confocal microscopy showed the DNA fragmentation in MCF-7 upon DCME treatment. Finally, the western blotting study revealed the induction of tumor suppressor protein, p53, which results in increasing the Bax/Bcl-2 ratio and activation of caspase-cascade pathways. Conclusion: The activation of caspase-3, -8, -9 and PARP degradation led us to conclude that DCME induces apoptosis in MCF-7 through both intrinsic and extrinsic mechanisms. The LC-MS analysis showed the presence of various bioactive compounds.

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