scholarly journals ANTIBACTERIAL ACTIVITY TEST OF EXTRACT NYAMPLUNG (Calophyllum inophyllum Linn.) LEAVES

2016 ◽  
Vol 11 (2) ◽  
pp. 200
Author(s):  
Mutiara Novianti ◽  
Qurrotul Aini ◽  
Irma Fadhila Putri
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Incubation Time ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Calophyllum Inophyllum ◽  
Optimum Activity ◽  
Flavonoid Compounds ◽  
Activity Test

Phytochemical screening and antibacterial activity from ethanol extract of nyamplung (<em>Callophylum</em><em> inophyllum</em> Linn.) leaves from Indonesia against <em>Escherichia</em><em> coli </em>has been done. The extraction was done by soklet extraction using ethanol. Antibacterial activity test using the <em>Optical Density</em> method at λmax 600 nm. The result of phytochemical screening from ethanol extract showed that there were flavonoid compounds, saponins, tannins, phenols and triterpenoids. The result of antibacterial activity test showed that the optimum activity occurs at incubation time of 3 hours with percentase inhibition persentase i.e. 59.03 %.

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF EXTRACT NYAMPLUNG (Calophyllum inophyllum Linn.) LEAVES

2016 ◽  
Vol 11 (2) ◽  
pp. 200
Author(s):  
Mutiara Novianti ◽  
Qurrotul Aini ◽  
Irma Fadhila Putri
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Incubation Time ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Calophyllum Inophyllum ◽  
Optimum Activity ◽  
Flavonoid Compounds ◽  
Activity Test

Phytochemical screening and antibacterial activity from ethanol extract of nyamplung (<em>Callophylum</em><em> inophyllum</em> Linn.) leaves from Indonesia against <em>Escherichia</em><em> coli </em>has been done. The extraction was done by soklet extraction using ethanol. Antibacterial activity test using the <em>Optical Density</em> method at λmax 600 nm. The result of phytochemical screening from ethanol extract showed that there were flavonoid compounds, saponins, tannins, phenols and triterpenoids. The result of antibacterial activity test showed that the optimum activity occurs at incubation time of 3 hours with percentase inhibition persentase i.e. 59.03 %.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

scholarly journals Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

2018 ◽  
Vol 1 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Dwi Latifah Sari ◽  
Sudarmi Sudarmi ◽  
Popi Patilaya
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

scholarly journals Antibacterial Activity Test of Ethanol Extract from Dried Simplicia Garlic (Allium sativum L.) Against Bacillus cereus Bacteria

2020 ◽  
Vol 3 (2) ◽  
pp. 93-99
Author(s):  
Athaillah Athaillah ◽  
Ugi Diana Lestari
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Allium Sativum ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Dried Extract

This research is aimed to find out the extract activity dried simplisia of garlic (Allium sativum L.) as an antibacterial which is capable to inhibiting growth of Bacillus cereus bacterium, to find out the alkaloid secondary of metabolites, flavonoid, tannin and saponin that contained in dried extract of garlic and to find out a concentration that has the most antibacterial activity which is capable to inhibiting growth of Bacillus cereus bacterium. Garlic was extracted by maceration method using a solvent ethanol 96%. After the extract obtaned, then phtochemical screening and standardization test. Thickening technique by evaporation used vacum rotary evaporator until thick extarct was obtained. Antibacterial activity thest by using disk diffusion method. This research was used seven concentrations which were 20% (b/v), 30% (b/v). 40% (b/v), 50% (b/v), 60% (b/v), 80% (b/v)and100% (b/v). The result of phytochemical screening test, ethanol extract garlic (Allium sativum L.) positive contained compound alkaloid, flavonoid, saponin and tannin. Standardization of power simplisia fulfill the requirements set by Depkes RI 2000. The result of activity test of optimum antibacterial was obtained inhibitory power with the number 27 mm on 40% (b/v) concentration. This proves that garlic (Allium sativum L.) have antibacterial affects against Bacillus cereus bacterium

scholarly journals Pengaruh kombinasi ekstrak etanol herba cecendet (Physalis angulata l.) dengan beberapa antibiotik terhadap bakteri Staphylococcus aureus dan Klebsiella pneumonie

2017 ◽  
Vol 5 (2) ◽  
pp. 50
Author(s):  
Anna Choirunnisa ◽  
Afifah Bambang Sutjiatmo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Physalis Angulata

<p align="center"><strong><em>The effect of a combination of ethanol extract of Physalis angulata L. with antibiotic against Staphylococcus aureus and Klebsiella pneumonie</em></strong></p><p><strong><em>                                                                                           </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Infectious disease is one of the biggest health problems not only in Indonesia, but worldwide. Bacteria are important agents in causing infectious diseases. Indonesian people often use antibiotics along with herbal medicines. Cecendet (Physalis angulata L.) is one of the traditional plants that have antibacterial activity and widely used by the Indonesian community. This study aims to test the antibacterial activity of cecendet herbs and their effects when used in combination with various antibiotics. The preparation of P.angulata extract was carried out using reflux method with ethanol 50%. The characteristic examination and phytochemical screening are examined on simplicia and the extract. Determination of antibacterial activity from P.angulata extract was done by microdilution test method by assessing minimum inhibitory concentration (MIC). Determination of the effectiveness of combination of antibiotics with herbal cecendet against test microbes Staphylococcus aureus and Klebsiella pneumoniae was done by using checkerboard method. The results of phytochemical screening showed that simplicia and P.angulata extract contained alkaloids, flavonoids, saponins, polyphenols, monoterpenoids, sesquiterpenes, steroids and triterpenoids. The result of antibacterial activity test showed that P.angulata extract had antibacterial activity against S.aureus and K.pneumoniae with the MIC was 128 μg/mL, 256 μg/mL. The synergistic interaction of S.aureus is demonstrated by the combination of P.angulata herb extract with tetracycline. The additive/indifferent interaction of S.aureus is demonstrated by the combination of P.angulata extracts with ampicillin, and against K.pneumoniae shown by the combination of P.angulata extract of cecendet with ampicillin or tetracycline. Ethanol extract of P.angulata has antibacterial activity and and combination with antibiotics (ampicillin/tetracyclin) can give synergistic effect or additive of S.aureus and K.pneumoniae bacteria.</em></p><p align="center"><strong><em> </em></strong></p><p><strong><em>Keywords</em></strong><em>:  Cecendet, Physalis angulata L., MIC, antibiotic combination</em></p>

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals In vitro Study on Anti-salmonella Activities of Boerhaavia diffusa (L. syn) Leaf Extract

2019 ◽  
pp. 1-10
Author(s):  
Ologun, Oluwabusayo ◽  
Dada, Ebenezer Oluyemi ◽  
Ajayi, Kehinde Oluyemi
Keyword(s):  
Antibacterial Activity ◽  
Clinical Isolate ◽  
Optical Density ◽  
Aqueous Extract ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Boerhaavia Diffusa ◽  
Broth Dilution

Various strategies have been employed in the treatment and management of Salmonella infection however, Salmonella strains have gained resistance to antibiotics. This study was to determine in vitro anti-Salmonella activity of Boerhaavia diffusa leaf extract against clinical isolate of Salmonella typhi and Salmonella typhi ATCC 14028. The aqueous and ethanol extracts of B. diffusa were studied for their antibacterial activity against pathogenic Salmonella typhi. This study was carried out between April and September 2018. The in vitro antibacterial activity was performed by agar well diffusion method and broth dilution using spectrophotometric method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well and optical density respectively. It was observed that aqueous extract exerted slightly higher activity than ethanolic extract as revealed by the mean diameter of zone of inhibitions at a concentration of 200 mg/ml, the aqueous extract had 35.21±0.47 mm (Salmonella typhi ATCC 14028) compared with ethanol extract 26.41±0.32 mm (clinical). However, in the broth dilution method, ethanol extract significantly (p=0.05) reduced the cell, at 48 hours, the optical density of clinical isolate of S. typhi treated at concentration of 200 mg/ml of extract was 0.47±0.02 nm while at the same concentration of extract, aqueous extract had an optical density of 0.52±0.11 nm respectively. The phytochemical assay revealed that tannin (5.18±0.02 mg/g) and quinone (8.45±0.13 mg/g) in ethanol extract was significantly (p=0.05) higher than aqueous extract while saponin (14.18±0.06 mg/g) was higher in the aqueous extract. The ethanol and aqueous extracts of leaves of B. diffusa whole plant exhibited significant antibacterial activity against both clinical and typed Salmonella typhi. Therefore, the plant extract could be used for the treatment of Salmonellosis, however, the in vivo studies is needed to ascertain the safety of the extract.

Aktivitas Antibakteri dari Ekstrak Etanol Ganoderma sp. Asal Pulau Lombok

2020 ◽  
Vol 6 (1) ◽  
pp. 22
Author(s):  
Adelina Oktaviani ◽  
Aida Muspiah ◽  
Faturrahman Faturrahman
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Large Diameter ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Gram Negative Bacteria ◽  
Activity Test

The use of antibiotics that are not according to the rules and antibiotics in the long term can cause resistance to bacteria. This study aims to determine the presence of antibacterial activity and the effect of increasing the concentration of ethanol extract of Ganoderma sp. against several test bacteria. Extract from Ganoderma sp. obtained by maceration method using ethanol 95% solvent. The extract concentrations used were 20%, 40%, 60% and 80%. This research was conducted using the wells method with ciprofloxacin as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition formed around the well. The results of the antibacterial activity test of ethanol extract Ganoderma sp. has greater inhibitory activity against gram-negative bacteria. The inhibitory activity of ethanolic extract of Ganoderma sp. on the growth of test bacteria increased with increasing concentration of the extract

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF BAWANG DAYAK (ELEUTHERINE SP.) AND HATI TANAH (ANGIOPTERIS SP.) AND THEIR COMBINATION AGAINST PROPIONIBACTERIUM ACNES

2019 ◽  
pp. 11-13
Author(s):  
SUSI NOVARYATIIN
Keyword(s):  
Antibacterial Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
The Other ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Other Hand ◽  
Diffusion Technique ◽  
Inhibition Zones

Objective: The objective of this research was to investigate the preliminary phytochemical screening and antibacterial activity of Bawang Dayak(Eleutherine sp.) and Hati Tanah (Angiopteris sp.) and their combination against Propionibacterium acnes.Methods: The extracts were used for phytochemical screening. Antibacterial activity was performed using disc diffusion technique, with twovariations of the concentration of 5% and 10% for each extracts, and combination of both extracts with three combinations: (1) 5%: 5%, (2) 5%: 10%, and (3) 10%: 5%.Results: Both extracts contained tannins, saponins, and steroids. The antibacterial activity against P. acnes showed that the inhibition zones ofBawang Dayak ethanol extract were 6.1±1.5 mm (5%) and 8.7±1.3 mm (10%). On the other hand, the inhibition zones of Hati Tanah ethanol extract were 4.0±1.6 mm (5%) and 9.2±2.5 mm (10%). The inhibition zones produced in combinations 1, 2, and 3 were 5.8±0.3 mm, 10.8±2.0 mm, and 15.5±2.8 mm, respectively.Conclusion: In this study showed the presence of tannins, saponins, and steroids that might be responsible for antibacterial activity in both extract. The best antibacterial activity was produced by combining the two extracts (combination 3).

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