scholarly journals Antibacterial Activity Test of Ethanol Extract from Dried Simplicia Garlic (Allium sativum L.) Against Bacillus cereus Bacteria

2020 ◽  
Vol 3 (2) ◽  
pp. 93-99
Author(s):  
Athaillah Athaillah ◽  
Ugi Diana Lestari
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Allium Sativum ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Dried Extract

This research is aimed to find out the extract activity dried simplisia of garlic (Allium sativum L.) as an antibacterial which is capable to inhibiting growth of Bacillus cereus bacterium, to find out the alkaloid secondary of metabolites, flavonoid, tannin and saponin that contained in dried extract of garlic and to find out a concentration that has the most antibacterial activity which is capable to inhibiting growth of Bacillus cereus bacterium. Garlic was extracted by maceration method using a solvent ethanol 96%. After the extract obtaned, then phtochemical screening and standardization test. Thickening technique by evaporation used vacum rotary evaporator until thick extarct was obtained. Antibacterial activity thest by using disk diffusion method. This research was used seven concentrations which were 20% (b/v), 30% (b/v). 40% (b/v), 50% (b/v), 60% (b/v), 80% (b/v)and100% (b/v). The result of phytochemical screening test, ethanol extract garlic (Allium sativum L.) positive contained compound alkaloid, flavonoid, saponin and tannin. Standardization of power simplisia fulfill the requirements set by Depkes RI 2000. The result of activity test of optimum antibacterial was obtained inhibitory power with the number 27 mm on 40% (b/v) concentration. This proves that garlic (Allium sativum L.) have antibacterial affects against Bacillus cereus bacterium

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN DAN KULIT BATANG BERENUK TERHADAP Bacillus cereus

2018 ◽  
Vol 15 (2) ◽  
pp. 31
Author(s):  
Devi Nisa Hidayati ◽  
Cicih Sumiarsih ◽  
Umroh Mahmudah ◽  
Anita Dwi Puspitasari
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Ethanol Extract ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Activity Measurements ◽  
Crescentia Cujete

ABSTRACTThe plants (Crecentia cujete Linn) Are known to have antibacterial activity and can be used as a diarrhea drug due to microorganisms. Bacillus cereus is one of the bacteria that causes diarrhea.This aims to the study was antibacterial activity of ethanol extract of leaves (EL) and bark broodstock (EB) on Bacillus cereus. EL and EB were extracted with ethanol 96% using maceration method. Antibacterial activity test was carried out with disk diffusion method in EL with a concentration of 30%, 60%, 90%, 120% and 150%, and in EB using concentrations of 60%, 70%, 80%, 90% and 100% then antibacterial activity measurements were taken. by looking at the Diameter of the Inhibitory Area (DDH). The results of the EL and EB of antibacterial activity proved that EL and EB berenuk have antibacterial activity against Bacillus cereus.Keywords: Antibacterial, Crescentia cujete Linn, Bacillus cereus.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine americana Merr) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella typhi

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 525
Author(s):  
Rafi’a Adinda Putri ◽  
Herny E.I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Paper Disk ◽  
Eleutherine Americana

ABSTRACTDayak onions (Eleutherine americana Merr) has been used as a traditional plant which has  benefits  for  many  diseases,  one  of  them  is  antibacterial. This study aims to determine the growth inhibition of ethanol extract of Dayak onions leaves on the bacterium S. aureus, E. coli and  S. typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby-Bauer diffusion). The results showed that ethanol extract of bawang dayak has the potential as an antibacterial and has antibacterial strength against S. aureus, E. coli and S. typhi. At concentrations of 20%, 40% and 60% forming inhibition zone diameters (16.23 mm, 19.18 mm, 21.25 mm) S. aureus, (10.7 mm, 13.98 mm, 15.87 mm) E. coli, and  (14.43 mm, 14.6 mm, 17.2 mm) S. typhi. At concentration of 20%, 40% and 60% included strong groups to inhibit E. coli and  S. typhi, while at concentration of 60%, it is included in a very strong group to inhibit the S. aureus bacteria. Keywords: Antibacterial activity, Bawang Dayak , Kirby-Bauer diffusion method   ABSTRAK Bawang Dayak (Eleutherine americana Merr) telah digunakan sebagai tanaman tradisional yang bermanfaat bagi banyak penyakit salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ekstrak etanol bawang dayak terhadap bakteri S. aureus, E.coli dan S. typhi menggunakan tiga kosentrasi yaitu 20%, 40% dan 60%. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan  menggunkan metode difusi dengan cakram kertas (difusi Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol bawang dayak berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap S. aureus, E. coli dan S. typhi. Pada konsentrasi 20%, 40% dan 60% membentuk diameter zona hambat (16.23 mm, 19.18 mm, 21.25 mm) pada S. aureus, (10.7 mm, 13.98 mm, 15,87 mm) pada E. coli, (14.43 mm, 14.6 mm, 17.2 mm) pada S. typhi. Pada konsentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri E. coli dan S. typhi, sedangkan pada konsentrasi 60% termasuk dalam golongan sangat kuat untuk menghambat bakteri S. aureus. Kata kunci: Aktivitas antibakteri, Bawang Dayak, metode difusi Kirby-Bauer

scholarly journals Antibacterial activity test of Simargaolgaol (Aglaonema modestum Schott ex Engl) leaves extract against Escherichia coli and Salmonella typhi bacteria

2021 ◽  
Vol 13 (2) ◽  
pp. 151-158
Author(s):  
Tiurma Solomasi Zega ◽  
◽  
Putri Mandaoni Pakpahan ◽  
Rahmayani Siregar ◽  
Givinda Sitompul ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Activity Test ◽  
Inhibitory Power ◽  
Ethanol Extracts

The Simargaolgaol (Aglaonema modestum Schott ex Engl) plant is a plant that grows wild in Barus District, Central Tapanuli, North Sumatra which is used by the community as a medicinal plant. This study aims to determine the antibacterial activity of n-hexane, ethyl acetate and ethanol extracts from Simargaolgaol leaves against Escherichia coli and Salmonella typhi bacteria. The antibacterial activity test of Simargaolgaol leaves was carried out using the disc diffusion method. The results of antibacterial activity showed that the highest inhibitory power of Simargaolgaol leaf extract was ethanol extract (polar), ethyl acetate extract (semi polar) and n-hexane extract (non polar). The inhibitory power of ethanol extract, ethyl acetate, and n-hexane against Escherichia coli bacteria was 13.1 mm (strong); 9.7 mm (medium); 8.0 mm (medium) and for Salmonella typhi bacteria respectively 11.2 mm (strong); 10.7 mm (strong) and 9.3 mm (medium). With the concentration of the extract in the diameter is 10%. Based on this, it can be concluded that Simargaolgaol leaf extract has potential as an antibacterial. Keywords: Aglaonema modestum Schott ex Engl, Antibacterial activity, Escherichia coli, Salmonella typhi

scholarly journals Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus (Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus)

2019 ◽  
Vol 9 (2) ◽  
pp. 83
Author(s):  
Ni Made Marlin Suarjo Putri ◽  
Febby Ester Fany Kandou ◽  
Marina Singkoh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Screening Test ◽  
Ethanol Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Inhibitory Power ◽  
E Mail

Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus(Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus) Ni Made Marlin Suarjo Putri1*), Febby Ester Fany Kandou1), Marina Singkoh1)1)Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan, Universitas Sam Ratulangi Manado*E-mail: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Penelitian ini bertujuan untuk menguji bioaktivitas antibakteri dari ekstrak etanol Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Pseudomonas aeruginosa dan Bacillus cereus dan mengetahui golongan senyawa yang terkandung di dalamnya. Tahapan penelitian meliputi pengambilan dan penyiapan sampel, identifikasi Gorgonia, ekstraksi, skrining fitokimia, pembuatan larutan Mc. Farland, pembuatan media dan pengujian antibakteri. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol. Pengujian bioaktivitas antibakteri menggunakan metode Kirby- Bauer. Hasil uji bioaktivitas antibakteri menunjukkan ekstrak etanol Mopsella sp. memiliki aktivitas antibakteri dengan kategori kuat (13,5 mm ± 2,60), Siphonogorgia sp. termasuk dalam kategori sedang (9 mm ± 1,80), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 0,87) pada bakteri Pseudomonas aeruginosa. Sedangkan pada bakteri uji Bacillus cereus, pada sampel Mopsella sp tidak memiliki daya hambat, sampel Siphonogorgia sp. termasuk dalam kategori sedang (8,3 mm ± 0,58), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 0,50).  Golongan senyawa yang terkandung dalam Mopsella sp. yaitu flavonoid, saponin, tanin. Golongan senyawa pada Siphonogorgia sp. yaitu alkaloid (Wagner) menunjukkan positif sedangkan golongan senyawa pada Villogorgia sp. yaitu alkaloid dan flavonoid.  Kata kunci:      skrining fitokimia, antibakteri, Mopsella sp., Siphonogorgia sp., Villogorgia sp. ABSTRACT This study aims to examine the antibacterial bioactivity of ethanol extract of Mopsella sp. * cf, Siphonogorgia sp. and Villogorgia sp. against Pseudomonas aeruginosa and Bacillus cereus and find out the class of compounds contained therein. The stages of the research included sampling and preparation, Gorgonia identification, extraction, phytochemical screening, making Mc solution. Farland, making media and antibacterial testing. Extraction was carried out by maceration using ethanol solvent. Antibacterial bioactivity testing using the Kirby-Bauer method. Antibacterial bioactivity test results showed that ethanol extract of Mopsella sp. had antibacterial activity with a strong category (13.5 mm ± 2.60), Siphonogorgia sp. included in the medium category (9 mm ± 1.80), Villogorgia sp. had no inhibitory power (6.5 mm ± 0,87) in the Pseudomonas aeruginosa bacteria. Whereas in Bacillus cereus test bacteria, in Mopsella sp. sample did not have inhibitory power, Siphonogorgia sp. sample was included in the medium category (8.3 mm ± 0.58), Villogorgia sp. did not have inhibitory power (6.5 mm ± 0,50). The groups of compounds contained in Mopsella sp. are flavonoids, saponins, tannins. The group of compounds in Siphonogorgia sp., namely alkaloids (Wagner) showed positivity while the compound groups in Villogorgia sp. were alkaloids and flavonoids.Key words: Phytochemical screening, Antibacterial, Mopsella sp., Siphonogorgia sp., Villogorgia sp.

scholarly journals Antibacterial Activity Test of Ethanol Extract and SAP of Betung Bamboo Shoot (Dendrocalamus asper) Against Klebsiella pneumoniae and Pseudomonas aeruginosa Bacteria

2021 ◽  
Vol 1 (1) ◽  
pp. 11
Author(s):  
Anif Nur Artanti ◽  
Farikatul Sufi Mujahidah
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Bamboo Shoot ◽  
Activity Test ◽  
Bamboo Shoots ◽  
Dendrocalamus Asper ◽  
Inhibitory Power

<p>Boiled water from bamboo shoots is often used for pneumonia and a cleanser for the wound. It is known that bamboo can be an antibacterial agent because it has saponin, alkaloid, and flavonoid. This research aims to study whether ethanol extract and sap from bamboo betung shoot (<em>Dendrocalamus asper</em>) has secondary metabolism and antibacterial activity against <em>Klebsiella pneumoniae</em> and <em>Pseudomonas aeruginosa</em>. The extract was taken by maceration method using ethanol 96%, and the sap was taken from the water of bamboo shoot. Antibacterial activity test was conducted using disc diffusion method to measure the magnitude of inhibitory power at six concentration ratios of b/v solutions (10%; 20%; 30%; 40%; 50%; 60%). The result showed that ethanol extract and sap of bamboo betung shoot contained saponin, quercetin, and quinine which were supposed to have antibacterial activity against <em>Klebsiella pneumoniae</em> and <em>Pseudomonas aeruginosa</em>. The largest inhibitory zone diameter was found in ethanol extract 60% with the category of moderate inhibition of 9.05 ± 0.12 mm against <em>Kliebsiella pneumoniae</em> and 5.07 ± 0.13 mm against <em>Pseudomonas aeruginosa</em>. While the sap with a concentration of 60% was included in the weak inhibitory category, which was 5.65 ± 0.05 mm in the <em>Kliebsiella pneumoniae</em> and 4.81 ± 0.22 mm in <em>Pseudomonas aeruginosa</em>.<strong></strong></p><p> </p>Keywords: antibacterial; bamboo shoot; betung bamboo.

Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus (Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus)

2019 ◽  
Vol 9 (2) ◽  
pp. 83
Author(s):  
Ni Made Marlin Suarjo Putri ◽  
Febby Kandou ◽  
Marina Singkoh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Screening Test ◽  
Ethanol Extract ◽  
Test Results ◽  
Phytochemical Screening ◽  
Inhibitory Power ◽  
E Mail

Skrining Fitokimia dan Uji Bioaktivitas Antibakteri dari Gorgonia Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Bakteri Pseudomonas aeruginosa dan Bacillus cereus(Phytochemical screening test the antibacterial bioactivity of gorgonia Mopsella sp. *cf, Siphonogorgia sp. and Villogorgia sp. Againts Pseudomonas aeruginosa and Bacillus cereus) Ni Made Marlin Suarjo Putri1*), Febby Ester Fany Kandou1), Marina Singkoh1)1)Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan, Universitas Sam Ratulangi Manado*E-mail: [email protected] Diterima 7 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Penelitian ini bertujuan untuk menguji bioaktivitas antibakteri dari ekstrak etanol Mopsella sp. *cf, Siphonogorgia sp. dan Villogorgia sp. terhadap Pseudomonas aeruginosa dan Bacillus cereus dan mengetahui golongan senyawa yang terkandung di dalamnya. Tahapan penelitian meliputi pengambilan dan penyiapan sampel, identifikasi Gorgonia, ekstraksi, skrining fitokimia, pembuatan larutan Mc. Farland, pembuatan media dan pengujian antibakteri. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol. Pengujian bioaktivitas antibakteri menggunakan metode Kirby- Bauer. Hasil uji bioaktivitas antibakteri menunjukkan ekstrak etanol Mopsella sp. memiliki aktivitas antibakteri dengan kategori kuat (13,5 mm ± 2,60), Siphonogorgia sp. termasuk dalam kategori sedang (9 mm ± 1,80), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 3,46) pada bakteri Pseudomonas aeruginosa. Sedangkan pada bakteri uji Bacillus cereus, pada sampel Mopsella sp tidak memiliki daya hambat, sampel Siphonogorgia sp. termasuk dalam kategori sedang (8,3 mm ± 0,58), Villogorgia sp. tidak memiliki daya hambat (6,5 mm ± 5,07).  Golongan senyawa yang terkandung dalam Mopsella sp. yaitu flavonoid, saponin, tanin. Golongan senyawa pada Siphonogorgia sp. yaitu alkaloid (Wagner) menunjukkan positif sedangkan golongan senyawa pada Villogorgia sp. yaitu alkaloid dan flavonoid.  Kata kunci:      skrining fitokimia, antibakteri, Mopsella sp., Siphonogorgia sp., Villogorgia sp. ABSTRACT This study aims to examine the antibacterial bioactivity of ethanol extract of Mopsella sp. * cf, Siphonogorgia sp. and Villogorgia sp. against Pseudomonas aeruginosa and Bacillus cereus and find out the class of compounds contained therein. The stages of the research included sampling and preparation, Gorgonia identification, extraction, phytochemical screening, making Mc solution. Farland, making media and antibacterial testing. Extraction was carried out by maceration using ethanol solvent. Antibacterial bioactivity testing using the Kirby-Bauer method. Antibacterial bioactivity test results showed that ethanol extract of Mopsella sp. had antibacterial activity with a strong category (13.5 mm ± 2.60), Siphonogorgia sp. included in the medium category (9 mm ± 1.80), Villogorgia sp. had no inhibitory power (6.5 mm ± 3.46) in the Pseudomonas aeruginosa bacteria. Whereas in Bacillus cereus test bacteria, in Mopsella sp. sample did not have inhibitory power, Siphonogorgia sp. sample was included in the medium category (8.3 mm ± 0.58), Villogorgia sp. did not have inhibitory power (6.5 mm ± 5.07). The groups of compounds contained in Mopsella sp. are flavonoids, saponins, tannins. The group of compounds in Siphonogorgia sp., namely alkaloids (Wagner) showed positivity while the compound groups in Villogorgia sp. were alkaloids and flavonoids.Key words: Phytochemical screening, Antibacterial, Mopsella sp., Siphonogorgia sp., Villogorgia sp.

scholarly journals Antibacterial Activity of Kecombrang Flower (Etlingera elatior (Jack) R.M. Sm) Extract against Staphylococcus epidermidis and Propionibacterium acnes

2021 ◽  
Vol 6 (1) ◽  
pp. 58528
Author(s):  
Vilya Syafriana ◽  
Renita Noviani Purba ◽  
Yayah Siti Djuhariah
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Activity Test ◽  
Etlingera Elatior

This study aimed to determine the antibacterial activity from the ethanol extract of Kecombrang flower (Etlingera elatior (Jack) R.M. Smith) against Staphylococcus epidermidis and Propionibacterium acnes. The extract was made by the maceration method with 70% ethanol as a solvent. Antibacterial activity test was carried out by the disk diffusion method with a concentration of 10%, 20%, 40%, and80%. Meanwhile, theMinimum InhibitoryConcentration(MIC)was done atconcentrations of 10%, 8%, 6%, 4%, and 2%. The results showed that the Kecombrang flower (Etlingera elatior (Jack) R.M.Smith) extract hadantibacterialactivityagainstS.epidermidisand P. acnes. The MIC for S. epidermidis is at a concentration of 4%, while in P.acnes cannot determine yet.

scholarly journals Antibacterial Activity of Leaf Extracts ofBaeckea frutescensagainst Methicillin-ResistantStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Baeckea Frutescens ◽  
High Degree

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA

Biomedika ◽  
2012 ◽  
Vol 4 (2) ◽  
Author(s):  
Dr. Muhtadi , MSi. ◽  
Ria Ambarwati ◽  
Ratna Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .

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