scholarly journals Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

2018 ◽  
Vol 1 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Dwi Latifah Sari ◽  
Sudarmi Sudarmi ◽  
Popi Patilaya
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KULIT BATANG MANGGA KWENI (Mangifera odorata Griff) TERHADAP Escherichia coli ATCC 25922 DAN Staphylococcus aureus ATCC 25923

2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Wuri Prihatiningtiyas ◽  
Yeni Mariani ◽  
H A Oramahi ◽  
Fathul Yusro ◽  
Lolyta Sisilia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Positive Control ◽  
Plate Count ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Plate Count Agar

The aims of this research are to determine the secondary metabolite content found in the ethanol extract of kweni mango bark (Mangifera odorata Griff) and analyze its potency as a natural antibacterial against Escherichia coli and Staphylococcus aureus. The research was started by maceration process using 96% ethanol solvent, then evaporated at 40-50oC and obtain yield of 20,61% with powder content of 8,34%. Furthermore, phytochemical screening was performed qualitatively to determine the secondary metabolite of the extract. The results showed that ethanol extract of M. odorata Griff bark contained secondary metabolites such as alkaloids, flavonoids, saponins, tannins, terpenoids and phenolics. In this study antibacterial activity was carried out using disc diffusion method in Plate Count Agar media and incubated for 24-48 hours. The results showed that the largest diameter of inhibitory zones formed at a concentration of 15 mg/ml for S. aureus was 12,33 mm with strong classified and for E. coli bacteria with a concentration of 200 mg/ml of 23,67 mm with very strong classified, and almost equal with the result shown by 30 µg tetracycline as positive control, which is 25 mm. The results of this study it can be concluded that the ethanol extract of the kweni mango bark (M. odorata Griff) is bacteriostatic.Keywords: antibacterial activity, Mangifera odorata Griff, phytochemical screening, Escherichia coli, Staphylococcus aureus.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BUAH MAHKOTA DEWA (Phaleria macrocarpa) TERHADAP STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 9 (02) ◽  
pp. 134-139
Author(s):  
Yunilda Rosa ◽  
Masnir Alwi ◽  
Monica Julianti
Keyword(s):  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Dilution Method ◽  
Phaleria Macrocarpa

Phaleria macrocarpa is one of the medicinal plants that many people use as a traditional medicinal which has many benefits, one of which is as a medicine for skin diseases. The content of flavonoid compounds in Phaleria macrocarpa believed to have potential as an antibacterial. The purpose of this study was to know the antibacterial activity of the ethanol extract of the Phaleria macrocarpa to inhibized Staphylococcus aureus bacteria. This research is an experimental study carried out by the liquid dilution method (KHM)and disk diffusion (KBM) of the extract of the Phaleria macrocarpa is divided into 6 concentrations namely 5%, 10% and 15% (KHM) and 40%, 50% and 60% (KBM). The positive control used is clindamycin and the negative control is aquadest. Data analysis uses univariate. Based on observations of the minimum inhibitory concentration of the extract of the fruit of the Phaleria macrocarpa which is at of 15%, while the minimum killed concentration is formed at of 40% with an average of 6.36 mm, and an average of zones formed on the positive control of 9.53 mm. From the results of the study, it was concluded that the extract of the fruit of the Phaleria macrocarpa can inhibit and kill the growth of Staphylococcus aureus bacteria.

scholarly journals Pengaruh kombinasi ekstrak etanol herba cecendet (Physalis angulata l.) dengan beberapa antibiotik terhadap bakteri Staphylococcus aureus dan Klebsiella pneumonie

2017 ◽  
Vol 5 (2) ◽  
pp. 50
Author(s):  
Anna Choirunnisa ◽  
Afifah Bambang Sutjiatmo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Physalis Angulata

<p align="center"><strong><em>The effect of a combination of ethanol extract of Physalis angulata L. with antibiotic against Staphylococcus aureus and Klebsiella pneumonie</em></strong></p><p><strong><em>                                                                                           </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Infectious disease is one of the biggest health problems not only in Indonesia, but worldwide. Bacteria are important agents in causing infectious diseases. Indonesian people often use antibiotics along with herbal medicines. Cecendet (Physalis angulata L.) is one of the traditional plants that have antibacterial activity and widely used by the Indonesian community. This study aims to test the antibacterial activity of cecendet herbs and their effects when used in combination with various antibiotics. The preparation of P.angulata extract was carried out using reflux method with ethanol 50%. The characteristic examination and phytochemical screening are examined on simplicia and the extract. Determination of antibacterial activity from P.angulata extract was done by microdilution test method by assessing minimum inhibitory concentration (MIC). Determination of the effectiveness of combination of antibiotics with herbal cecendet against test microbes Staphylococcus aureus and Klebsiella pneumoniae was done by using checkerboard method. The results of phytochemical screening showed that simplicia and P.angulata extract contained alkaloids, flavonoids, saponins, polyphenols, monoterpenoids, sesquiterpenes, steroids and triterpenoids. The result of antibacterial activity test showed that P.angulata extract had antibacterial activity against S.aureus and K.pneumoniae with the MIC was 128 μg/mL, 256 μg/mL. The synergistic interaction of S.aureus is demonstrated by the combination of P.angulata herb extract with tetracycline. The additive/indifferent interaction of S.aureus is demonstrated by the combination of P.angulata extracts with ampicillin, and against K.pneumoniae shown by the combination of P.angulata extract of cecendet with ampicillin or tetracycline. Ethanol extract of P.angulata has antibacterial activity and and combination with antibiotics (ampicillin/tetracyclin) can give synergistic effect or additive of S.aureus and K.pneumoniae bacteria.</em></p><p align="center"><strong><em> </em></strong></p><p><strong><em>Keywords</em></strong><em>:  Cecendet, Physalis angulata L., MIC, antibiotic combination</em></p>

scholarly journals ANTIBACTERIAL ACTIVITY OF KARAMUNTING (RHODOMYRTUS TOMENTOSA (AITON) HASSK) LEAF EXTRACT AND FRACTIONS

2018 ◽  
Vol 11 (3) ◽  
pp. 163
Author(s):  
Siska Esperanza Sinulingga ◽  
Poppy Anjelisa Zaitun Hasibuan ◽  
Dwi Suryanto
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Rhodomyrtus Tomentosa ◽  
Agar Well Diffusion Method

 Objective: This study was to analyze the karamunting leaf extract and fractions effectiveness against Staphylococcus aureus and Pseudomonas aeruginosa infection.Methods: Phytochemical screening is done in the extract and fractions of karamunting leaf continue with Standard agar well diffusion method for S. aureus and P. aeruginosa. Dimethyl sulfoxide was used as negative control, and standard antibiotic Kalmicetine (chloramphenicol) was used as positive control. Result: The ethyl acetate fraction showed the highest antibacterial activity against S. aureus and P. aeruginosa. Conclusion: Karamunting leaf can be used to treat the bacterial infection diseases especially infection from S. aureus and P. aeruginosa.

scholarly journals Antibacterial Activities Test of Combination of Ethanolic Extract of Betel Leaves (Piper betle L.) and Basil Leaves (Ocimum basilicum L.) Against Staphylococcus aureus

2018 ◽  
Vol 9 (2) ◽  
pp. 118-126
Author(s):  
Junie Suriawati ◽  
Patimah Patimah ◽  
Siti Rahayu Rachmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Piper Betle ◽  
Betel Leaf

Betel leaf extract and basil leaf extract contain phenol compounds, safonin, flavonoids, tannins, and essential oils that act as antibacterials. Skin is very susceptible to infection or other skin diseases one of which is caused by Staphylococcus aureus bacteria. This research needs to be done to test the antibacterial activity of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The process of extraction of betel leaf and basil leaves was done with 96% ethanol solvent using maceration method. The combinations used in the sample solution were the concentration of ethanol extract of green betel leaf (0%, 15%, 30%, 45%, 60%, 75%) and basil leaf extract (0%, 15%, 30%, 45%, 60 %, 75%). Furthermore, the antibacterial activity was tested for S. aureus with well diffusion method. The data obtained were tested statistically using Anova and Duncan's advanced test. The result of statistical analysis showed difference of drag zone (p <0,05) between various concentration of ethanol extract of green betel leaf, basil leaf and combination of both extracts to S. aureus. The smallest resin zone of ethanol extract of  betel leaf and ethanol extract of basil leaves is at 0% concentration (S0 and K0) and highest at 75% concentration (S5 and K5). While the smallest zone of inhibition for the combination of ethanol extract of betel leaf and ethanol extract of basil leaves at concentration S0K0, S0K1 and S0K2 is 0 mm and highest at S5K5 concentration is 31,3 mm. The higher concentration of betel leaf extract, basil leaf extract and the combination of the two extracts, the inhibition of diameter zone will be better. It can be concluded that the combination of  betel leaf extract and basil leaf extract has the ability to inhibit the growth of S. aureus better than single extract.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEREHAU (Callicarpa longifolia Lam) TERHADAP Escherichia coli dan Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 166
Author(s):  
Eko Kusumawati ◽  
Anita Apriliana ◽  
Khusnul Khatimah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Test ◽  
Test Concentration ◽  
And Staphylococcus Aureus ◽  
One Way Anova

Kerehau (Callicarpa longifolia Lam) is a plant that has traditionally efficacious as a cure colds and diarrhea. Part used as medicine are the leaves. This study aims to determine the antibacterial activity of ethanol extract of leaves kerehau on Escherichia coli and Staphylococcus aureus. Kerehau leaf extract prepared by maceration method using ethanol 70%, with a test concentration of 10%, 25%, 50%, and 100% and chloramphenicol 0.1% (w / v) as a positive control and dimethyl sulfoxide 1% (v / v) as a negative control. The results of antibacterial activity test with extract concentrations of 25%, 50% and 100% in Escherichia coli is 9.45 mm, 12.21 mm and 17.38 mm, for the positive control is 21.85 mm. The results of antibacterial activity test with extract concentrations of 10%, 25%, 50% and 100% in Staphylococcus aureus was 7.3 mm, 9.85 mm, 13.65 mm and 20.58 mm, for the positive control was 24.05 mm. The antibacterial activity was analyzed by One Way ANOVA showed significant differences between treatments

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals ANTI-BACTERIAL ACTIVITY TEST OF ETHANOL EXTRACTS AND ETHLACETATE FRACTION FROM THE EXTRACT OF JATROPHA CURCAS L. LEAVES AGAINST STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 5 (1) ◽  
pp. 31
Author(s):  
Sekar Wulandari
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Diffusion Method ◽  
Resistance Response ◽  
Activity Test ◽  
Significant Difference

Background: Staphylococcus aureus is a gram-positive bacteria that can cause infection. One of the plants that has antibacterial activity is jatropha leaves which contain flavonoids, saponins, tannins, alkaloids, steroids and polyphenols. Purpose: To determine the antibacterial activity of ethanol extract and ethyl acetate fraction with concentrations of 30%, 60% and 100% against Staphylococcus aureus bacteria. Method: The method for extracting jatropha leaves is maceration with 96% ethanol solvent and the fractionation method, namely liquid-liquid fractionation with ethyl acetate solvent. Antibacterial activity test was carried out in vitro with the disc diffusion method and compared the mean zone of inhibition of each treatment with a positive control (gentamicin 10 μg). Result: The results showed that the ethanol extract and ethyl acetate fraction of jatropha leaves had a strong resistance response, while the positive control gave a very strong inhibitory response to the growth of S. aureus bacteria. Based on the one way ANOVA test, ethanol extract and ethyl acetate fraction showed a significant difference from each treatment with a significant value (P=<0.05). Conclusion: The ethanol extract of Jatropha leaves can inhibit the growth of S. aureus bacteria at a concentration of 100% (18.28 ± 0.50 mm), 100% concentration of ethyl acetate fraction (15.10 ± 0.12 mm). The ethanol extract provided the best inhibition power, namely 18.28 ± 0.50 mm and a positive control 21.82 ± 0.092 mm.

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