scholarly journals Pengaruh kombinasi ekstrak etanol herba cecendet (Physalis angulata l.) dengan beberapa antibiotik terhadap bakteri Staphylococcus aureus dan Klebsiella pneumonie

2017 ◽  
Vol 5 (2) ◽  
pp. 50
Author(s):  
Anna Choirunnisa ◽  
Afifah Bambang Sutjiatmo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Physalis Angulata

<p align="center"><strong><em>The effect of a combination of ethanol extract of Physalis angulata L. with antibiotic against Staphylococcus aureus and Klebsiella pneumonie</em></strong></p><p><strong><em>                                                                                           </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Infectious disease is one of the biggest health problems not only in Indonesia, but worldwide. Bacteria are important agents in causing infectious diseases. Indonesian people often use antibiotics along with herbal medicines. Cecendet (Physalis angulata L.) is one of the traditional plants that have antibacterial activity and widely used by the Indonesian community. This study aims to test the antibacterial activity of cecendet herbs and their effects when used in combination with various antibiotics. The preparation of P.angulata extract was carried out using reflux method with ethanol 50%. The characteristic examination and phytochemical screening are examined on simplicia and the extract. Determination of antibacterial activity from P.angulata extract was done by microdilution test method by assessing minimum inhibitory concentration (MIC). Determination of the effectiveness of combination of antibiotics with herbal cecendet against test microbes Staphylococcus aureus and Klebsiella pneumoniae was done by using checkerboard method. The results of phytochemical screening showed that simplicia and P.angulata extract contained alkaloids, flavonoids, saponins, polyphenols, monoterpenoids, sesquiterpenes, steroids and triterpenoids. The result of antibacterial activity test showed that P.angulata extract had antibacterial activity against S.aureus and K.pneumoniae with the MIC was 128 μg/mL, 256 μg/mL. The synergistic interaction of S.aureus is demonstrated by the combination of P.angulata herb extract with tetracycline. The additive/indifferent interaction of S.aureus is demonstrated by the combination of P.angulata extracts with ampicillin, and against K.pneumoniae shown by the combination of P.angulata extract of cecendet with ampicillin or tetracycline. Ethanol extract of P.angulata has antibacterial activity and and combination with antibiotics (ampicillin/tetracyclin) can give synergistic effect or additive of S.aureus and K.pneumoniae bacteria.</em></p><p align="center"><strong><em> </em></strong></p><p><strong><em>Keywords</em></strong><em>:  Cecendet, Physalis angulata L., MIC, antibiotic combination</em></p>

scholarly journals Pengaruh kombinasi ekstrak etanol herba cecendet (Physalis angulata l.) dengan beberapa antibiotik terhadap bakteri Staphylococcus aureus dan Klebsiella pneumonie

2018 ◽  
Vol 5 (2) ◽  
pp. 50
Author(s):  
Anna Choirunnisa ◽  
Afifah Bambang Sutjiatmo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Physalis Angulata

<p align="center"><strong>Abstrak</strong></p><p> </p><p>Penyakit infeksi merupakan salah satu masalah kesehatan terbesar tidak saja di Indonesia, namun di seluruh dunia. Bakteri merupakan agen penting dalam menyebabkan penyakit infeksi. Seringkali masyarakat Indonesia menggunakan antibiotik bersamaan dengan penggunaan obat herbal. Cecendet (<em>Physalis angulata</em> L.) merupakan salah satu tanaman tradisional yang memiliki aktivitas  antibakteri dan banyak digunakan oleh masyakat Indonesia secara turun menurun. Penelitian ini  bertujuan untuk menguji aktivitas antibakteri herba cecendet dan pengaruhnya jika digunakan kombinasi dengan antibiotik. Pembuatan ekstrak herba cecendet dilakukan menggunakan metode refluks dengan pelarut etanol 50%. Terhadap simplisia dan ekstrak yang diperoleh, dilakukan pemeriksaan karakteristik dan penapisan fitokimia. Penentuan aktivitas antibakteri dari ekstrak herba cecendet dilakukan dengan metode uji mikrodilusi dengan menilai konsentrasi hambat minimum (KHM). Penentuan efektivitas kombinasi  ekstrak herba cecendet dengan antibiotik (ampisilin/tetrasiklin) terhadap <em>Staphylococcus aureus dan Klebsiella pneumoniae</em> dilakukan dengan menggunakan metode papan catur (<em>Checkerboard</em>).  Hasil penapisan fitokimia menunjukkan bahwa simplisia dan ekstrak etanol herba cecendet mengandung alkaloid, flavonoid, saponin, polifenol, monoterpenoid, seskuiterpen, steroid dan triterpenoid. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak etanol herba cecendet mempunyai aktivitas antibakteri terhadap <em>S.aureus dan K.pneumoniae</em> dengan konsentrasi hambat minimum berturut-turut adalah 128 µg/mL, 256 µg/mL. Interaksi yang sinergis terhadap <em>S.aureus</em> ditunjukkan oleh kombinasi ekstrak herba cecendet dengan tetrasiklin. Interaksi yang aditif/indifferent terhadap <em>S.aureus</em> ditunjukkan oleh kombinasi ekstrak herba cecendet dengan ampisilin, dan terhadap <em>K.pneumoniae</em> ditunjukkan oleh kombinasi ekstrak herba cecendet dengan ampisilin atau tetrasiklin. Ekstrak etanol herba cecendet memiliki aktivitas antibakteri dan dan kombinasi dengan antibiotik (ampisilin/tetrasiklin) dapat memberikan efek sinergis atau aditif bakteri <em>S.aureus</em> dan <em>K.pneumoniae.</em></p><p> </p><p><strong>Kata kunci:  </strong>Cecendet, <em>Physalis angulata</em> L., konsentasi hambat minimum, antibakteri, kombinasi antibiotik</p><p> </p><p> </p><p align="center"><strong><em>The effect of a combination of ethanol extract of Physalis angulata L. with antibiotic against Staphylococcus aureus and Klebsiella pneumonie</em></strong></p><p><strong><em>                                                                                           </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p>Infectious disease is one of the biggest health problems not only in Indonesia, but worldwide. Bacteria are important agents in causing infectious diseases. Indonesian people often use antibiotics along with herbal medicines. Cecendet (<em>Physalis angulata</em> L.) is one of the traditional plants that have antibacterial activity and widely used by the Indonesian community. This study aims to test the antibacterial activity of cecendet herbs and their effects when used in combination with various antibiotics. The preparation of <em>P.angulata</em> extract was carried out using reflux method with ethanol 50%. The characteristic examination and phytochemical screening are examined on simplicia and the extract. Determination of antibacterial activity from <em>P.angulata</em> extract was done by microdilution test method by assessing minimum inhibitory concentration (MIC). Determination of the effectiveness of combination of antibiotics with herbal cecendet against test microbes <em>Staphylococcus aureus</em> and <em>Klebsiella pneumoniae</em> was done by using checkerboard method. The results of phytochemical screening showed that simplicia and <em>P.angulata</em> extract contained alkaloids, flavonoids, saponins, polyphenols, monoterpenoids, sesquiterpenes, steroids and triterpenoids. The result of antibacterial activity test showed that <em>P.angulata</em> extract had antibacterial activity against <em>S.aureus</em> and <em>K.pneumoniae</em> with the MIC was 128 μg/mL, 256 μg/mL. The synergistic interaction of <em>S.aureus</em> is demonstrated by the combination of <em>P.angulata</em> herb extract with tetracycline. The additive/indifferent interaction of <em>S.aureus</em> is demonstrated by the combination of <em>P.angulata</em> extracts with ampicillin, and against <em>K.pneumoniae</em> shown by the combination of <em>P.angulata</em> extract of cecendet with ampicillin or tetracycline. Ethanol extract of <em>P.angulata</em> has antibacterial activity and and combination with antibiotics (ampicillin/tetracyclin) can give synergistic effect or additive of<em> S.aureus</em> and <em>K.pneumoniae</em> bacteria.</p><p align="center"><strong><em> </em></strong></p><p><strong>Keywords</strong>: Cecendet, <em>Physalis angulata</em> L., MIC, antibiotic combination</p>

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 96% DAUN MIMBA (Azadirachta indica A. Juss. ) DENGAN METODE EKSTRAKSI PERKOLASI TERHADAP PERTUMBUHAN BAKTERI

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Yanu Andhiarto ◽  
Rina Andayani ◽  
Nur Hidayatul Ilmiyah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Skin Inflammation ◽  
Screening Tests ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Activity Test

Skin infections most often found in developing countries, especially in the tropics, are purulent skin inflammation (pyoderma), the main cause of Staphylococcus aureus. The increasing number of cases of bacteria that are resistant to antibacterial can encourage the extraction of sources of antibacterial drugs from natural ingredients. One of the plants that is thought to have the antibacterial activity of Staphylococcus aureus is the Neem plant. In this study an antibacterial activity test was carried out with the well diffusion method from 96% ethanol extract of Mimba leaves extracted by percolation method. Previously 96% ethanol extract from Mimba leaves had been identified by secondary metabolites by phytochemical screening and standardized extract testing. Standarization using non-specific parameters (moisture content, ash content and drying losses) found that 96% ethanol extract of Mimba leaves entered a range of standard parameters and in phytochemical screening tests obtained 96% ethanol extract of Mimba leaves containing alcaloid, saponins, tannins, steroids / terpenoids and flavonoids. The results of the study on the antibacterial activity test showed a concentration of 75% ethanol extract of 96% Mimba leaves classified as having strong activity, at a concentration of 50% and 25% classified as having moderate activity. Based on this study it can be concluded that the three concentrations of 96% ethanol extract of Mimba leaves were able to inhibit the growth of Staphylococcus aureus bacteria.

scholarly journals Test Activities Antibakteriekstrak Ethanol Young And Old Leaves Of The Soursop(Annonamuricata L.) Against Staphylococcus aureus

2018 ◽  
Vol 1 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Dwi Latifah Sari ◽  
Sudarmi Sudarmi ◽  
Popi Patilaya
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Skin Diseases ◽  
Ethanol Extract ◽  
Positive Control ◽  
Annona Muricata ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Young Leaves

Soursop (Annona muricata L.) has been used for generations by some communities in Indonesia to treat diseases. Soursop leaves are used as an alternative treatment for cancer, by boiling them in the water and consuming it. In addition to the treatment of cancer, soursop plant is also used for the treatment of skin diseases such as boils. This study aimed to determine the antibacterial activity of ethanol extract of soursop leaves of young and old against Staphylococcus aureus, determine the content of secondary metabolites, and find out the diameter of inhibitory young leaves and old leaves of soursop in inhibiting Staphylococcus aureus. Soursop leaves were extracted by maceration using ethanol 96%. The extract obtained was then carried out to phytochemical screening. Antibacterial activity test was conducted using disc the Kirby-Bauerdiffusion. This study used 6 concentrations of 500 mg/mL, 400 mg/mL, 300 mg/mL, 200 mg/mL, 100 mg/mL, 80 mg/mL, 60 mg/mL, 40 mg/mL, 20 mg/mL, 10 mg/mL and 1 mg/mL. A positive control used soursop leaf extract of young and old while a negative one used DMSO 10%. Based on phytochemical screening, ethanol extract of soursop leaves contained alkaloids, flavonoids, saponins, tannins, steroids/triterpenoids and glycosides. The antibacterial activity of ethanol extract of leaves of young soursop was higher in inhibitory diameter than the ethanol extract of old soursop leaves. It could be seen that a concentration of 500 mg/mL was equal to 10.87 mm and 8.68 mm; a concentration of 400 mg/mL was equal to 9.15 mm and 7.3 mm; a concentration of 300 mg/mL was equal to 8.34 mm and 6.30 mm; a concentration of 200 mg/mL was equal to 8.23 mm and 7.08 mm; a concentration of 100 mg/mL was equal to 6.32 mm and 6.18 mm, and a concentration of 80 mg/mL did not leave a diameter inhibitory to bacteria Staphylococcus aureus. Young soursop leaf ethanol extract was more effective than the old soursop one against Staphylococcus aureus.

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 90-97
Author(s):  
Gaby Maulida Nurdin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Lantana Camara ◽  
Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Growth Inhibitory ◽  
One Way Anova

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn)  on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

scholarly journals PROFIL KANDUNGAN KIMIA DAN AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN BAMBAN (Donax canniformis (G. Forst.) K. Schum.) TERHADAP Staphylococcus aureus

2015 ◽  
Vol 1 (2) ◽  
pp. 141-148
Author(s):  
Hidayatullah Hidayatullah ◽  
Syariful Anam ◽  
Muhamad Rinaldhi Tandah
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Minimum Bactericidal Concentration ◽  
Dilution Method ◽  
The Family

Bamban (Donax canniformis (G. Forst.) K. Schum.) is one of the family Marantaceae plant that has many uses such as traditional medicine. Methanol extract of bamban leaves contains phenolic, tannins and saponins compounds. The purpose of this research is to determine the class of compounds that has antibacterial activity against Staphylococcus aureus and determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methanol extract of bamban leaves. This extract was prepared using maceration method with methanol solvent. Determination the class of compounds was initiated by bioautografi test in order to determine spots which has have antibacterial activity. Subsequently, the spot were identified the class of compound using reagent spray FeCl3 and H2SO4 10%. The determination of MIC and MBC using dilution method. Research showed there are three compounds that had antibacterial activity. These compounds were predicted as spot I and spot II which were phenolic compounds and spot III as a saponin compound. MIC and MBC value of the methanol extract of leaves bamban leaves 8% and 13%, respectively.

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI KRIM EKSTRAK ETANOL HERBA SELEDRI (Apium graveolens L.) TERHADAP BAKTERI Staphylococcus aureus

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 226
Author(s):  
Garry Clements ◽  
Paulina V. Y. Yamlean ◽  
Widya Astuty Lolo
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Apium Graveolens ◽  
Dilution Method ◽  
Herb Extract

ABSTRACTCelery herbs (Apium graveolens L.) have content flavonoid, saponin and tannin. Flavonoids have anti-inflammatory properties, antioxidants and antibacterials. This study aims to determine whether the ethanol extract of celery herbs can be formulated into cream preparations, test the quality of preparations at concentrations of 5%, 10% and 15%. These methods of research are experimental laboratories by making the supply of self-sustaining herbal celery ethanol extract cream and testing the quality of the cream preparation,  as well as testing of antibacterial activity was carried out using the liquid dilution method and measuring value of  Minimum Inhibitory Concentration  and Minimum Bactericidal Concentration using a UV-Vis Spectrophotometer. This research shows that celery herbs ethanol extract can be formulated into cream preparations, fulfilling quality test of organoleptic, scatter power, adhesion and cycling test, but that doesn’t meet the requirements for homogeneity tests and pH tests and has antibacterial activity which not only inhibits but kills bacteria in preparations of 5% and 15%Keywords: Cream Celery herb extract, Antibacterial, Staphylococcus aureus, UV-Vis spectrophotometer ABSTRAK Herba Seledri (Apium graveolens L.) memiliki kandungan flavonoid, saponin dan tannin. Flavonoid memiliki khasiat sebagai antiinflamasi, antioksidan dan antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, menguji mutu sediaan pada konsentrasi 5%, 10% dan 15%. Metode penelitian ini experimental laboratorium dengan membuat Sediaan Krim Ekstrak Etanol Herba Seledri dan menguji mutu sediaan krim, serta   menguji aktivitas antibakteri menggunakan metode dilusi cair dan mengukur nilai KBM dan KHM menggunakan Spektrofotometer UV-Vis. Hasil penelitian menujukkan bahwa ekstrak etanol herba Seledri dapat diformulasikan menjadi sediaan krim, memenuhi uji mutu sediaan organoleptik, daya, sebar, daya hambat dan stabilitas  namun tidak memenuhi persyaratan untuk uji homogenitas dan uji pH serta mempunyai aktivitas antibakteri yang membunuh bakteri pada sediaan berkonsentrasi 5% dan 15%.Kata Kunci : Krim Ekstrak herba Seledri, Antibakteri, Staphylococcus aureus, Spektrofotometer UV-Vis

scholarly journals Uji Daya Hambat Ekstrak Etanol, Fraksi n-heksana dan Etanol Daun Ceremai (Phyllanthus acidus L.) Skeels) Terhadap Salmonella thypi

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
Sernita Sernita ◽  
Irnawati Irnawati ◽  
Syamsinar Syamsinar
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Test Method ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Herbal Plants ◽  
Phyllanthus Acidus ◽  
Ethanol Fraction

ABSTRAK Penyakit yang sering diobati dengan tanaman herbal adalah infeksi yang disebabkan oleh bakteri. Salah satu tanaman yang dapat dimanfaatkan sebagai obat herbal adalah daun ceremai. Penelitian ini bertujuan untuk mengetahui metabolit sekunder dan zona hambat ekstrak etanol, fraksi n-heksana dan etanol daun ceremai (Phyllanthus acidus L.) skeels) terhadap Salmonella thypi. Penyarian daun ceremai dengan metode maserasi menggunakan pelarut etanol 96% dilanjutkan fraksinasi dengan pelarut n-heksana dan etanol. Hasil ekstrak dan fraksi daun ceremai kemudian dilakukan uji skrining fitokimia untuk mengetahui senyawa metabolit sekunder yang terdapat dalam sampel. Metode pengujian aktivitas antibakteri yang digunakan dalam penelitian ini adalah metode sumuran. Berdasarkan hasil penelitian, skrining fitokimia ekstrak etanol dan fraksi etanol mengandung senyawa saponin, tanin, flavonoid dan alkaloid sedangkan fraksi n-heksana mengandung senyawa tanin, flavonoid dan alkaloid. Aktivitas antibakteri metode sumuran ekstrak dan fraksi daun ceremai dengan konsentrasi 15%, 20%, dan 25% memiliki diameter rata-rata zona hambat secara berturut-turut adalah ekstrak etanol yaitu 13,24 mm, 13,74 mm, dan 14,13 mm, Fraksi n-heksan 13,806 mm, 14,32 dan 14,763 mm dan Fraksi etanol 13,096 mm, 13,416 mm, dan 13,486 mm. Hasil identifikasi aktivitas antibakteri ekstrak etanol, fraksi n-heksana dan fraksi etanol menunjukkan fraksi n-heksana memiliki zona hambat yang paling tinggi walaupun masih tergolong dalam zona hambat kategori lemah. Kata Kunci: Daun Ceremai, Ekstrak Etanol, Fraksi n-Heksana, Fraksi Etanol Salmonella thypi. ABSTRACT Diseases that are often treated with herbal plants are infections caused by bacteria. One plant that can be used as an herbal medicine is ceremai leaves. This study aims to determine secondary metabolites and inhibitory zones of ethanol extract, n-hexane fraction and etanol fraction of ceremai leaves (Phyllanthus acidus L.) Skeels) on Salmonella thypi. The extraction of ceremai leaves by maceration method used 96% ethanol was continued by fractionation with n-hexane and ethanol solvents. The results of extracts and fraction of ceremai leaves were then carried out by phytochemical screening test to determine the secondary metabolites found in the sample. Antibacterial activity test method used in this study is the method of wells. Based on the results of the study, phytochemical screening of ethanol extract and ethanol fraction contained saponin, tannin, flavonoid and alkaloid compounds while the n-hexane fraction contained tannin, flavonoid and alkaloid compounds. Antibacterial activity of wells method of ceremai leaf extract and fraction with a concentration of 15%, 20%, and 25% had an average inhibitory zone diameter, respectively, of ethanol extract 13,24 mm, 13,74 mm, and 14,13 mm, n-hexane fraction 13,806 mm, 14,32 mm and 14,763 mm and ethanol fraction 13,096 mm, 13,416 mm, and 13,486 mm. The results of the identification of antibacterial activity of ethanol extract, n-hexane fraction and ethanol fraction showed that the n-hexane fraction had the highest inhibition zone although it was still classified as a weak inhibition zone. Keywords : Ceremai Leaf, Ethanol Extract, n-Hexane Fraction, Salmonella thypi.

scholarly journals ANTIBACTERIAL ACTIVITY TEST OF EXTRACT NYAMPLUNG (Calophyllum inophyllum Linn.) LEAVES

2016 ◽  
Vol 11 (2) ◽  
pp. 200
Author(s):  
Mutiara Novianti ◽  
Qurrotul Aini ◽  
Irma Fadhila Putri
Keyword(s):  
Antibacterial Activity ◽  
Optical Density ◽  
Incubation Time ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Calophyllum Inophyllum ◽  
Optimum Activity ◽  
Flavonoid Compounds ◽  
Activity Test

Phytochemical screening and antibacterial activity from ethanol extract of nyamplung (<em>Callophylum</em><em> inophyllum</em> Linn.) leaves from Indonesia against <em>Escherichia</em><em> coli </em>has been done. The extraction was done by soklet extraction using ethanol. Antibacterial activity test using the <em>Optical Density</em> method at λmax 600 nm. The result of phytochemical screening from ethanol extract showed that there were flavonoid compounds, saponins, tannins, phenols and triterpenoids. The result of antibacterial activity test showed that the optimum activity occurs at incubation time of 3 hours with percentase inhibition persentase i.e. 59.03 %.

scholarly journals Antibacterial activity testing of ethanolic extract of aloe vera leaf and gel against methicillin-resistant Staphylococcus aureus

2014 ◽  
Vol 26 (2) ◽  
Author(s):  
Tias Eka Pratiwi ◽  
Warta Dewi ◽  
Eddy Prijono
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Test Method ◽  
Methicillin Resistant ◽  
Aloe Vera Gel ◽  
Significant Difference

Introduction: The interest on Aloe vera based herbal products is fast growing during recent years, particularly in medication regarding their antibacterial properties which had a lot of active components such as saponin and anthraquinone. Methicillin-resistant Staphylococcus aureus has emerged as one of the most important human pathogens causing nosocomial infections and became the first priority as a consequence of their resistancy. The purpose of this study is to determine the minimum levels of ethanol extract of leaves and Aloe Vera gel that can inhibit MRSA and find out whether there are differences in antibacterial power between of ethanol extract of leaves and Aloe Vera gel. Methods: Ten isolates of MRSA were investigated for their sensitivity to Aloe vera leaf and gel extract using the serial dilution method by doing two times repetition and statistically analyzed according to t-test method. Result: The result shows that the Minimum Inhibitory Concentration of Aloe vera leaf and gel extract each located at a concentration of 25% and 12,5%. There is a significant difference in antibacterial power between the ethanol extract of leaves and Aloe Vera gel in inhibiting MRSA with MIC values of 25% and 12.5% Conclusion: There was a difference between Aloe vera leaf and gel extract antibacterial activity, where the gel extract is more effective than leaf extract.

Sign in / Sign up

Export Citation Format

Share Document