Aktivitas Antibakteri dari Ekstrak Etanol Ganoderma sp. Asal Pulau Lombok

2020 ◽  
Vol 6 (1) ◽  
pp. 22
Author(s):  
Adelina Oktaviani ◽  
Aida Muspiah ◽  
Faturrahman Faturrahman
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Activity ◽  
Large Diameter ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Gram Negative Bacteria ◽  
Activity Test

The use of antibiotics that are not according to the rules and antibiotics in the long term can cause resistance to bacteria. This study aims to determine the presence of antibacterial activity and the effect of increasing the concentration of ethanol extract of Ganoderma sp. against several test bacteria. Extract from Ganoderma sp. obtained by maceration method using ethanol 95% solvent. The extract concentrations used were 20%, 40%, 60% and 80%. This research was conducted using the wells method with ciprofloxacin as a positive control and 50% DMSO as a negative control. The parameter measured is the large diameter of the inhibition formed around the well. The results of the antibacterial activity test of ethanol extract Ganoderma sp. has greater inhibitory activity against gram-negative bacteria. The inhibitory activity of ethanolic extract of Ganoderma sp. on the growth of test bacteria increased with increasing concentration of the extract

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEREHAU (Callicarpa longifolia Lam) TERHADAP Escherichia coli dan Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 166
Author(s):  
Eko Kusumawati ◽  
Anita Apriliana ◽  
Khusnul Khatimah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Activity Test ◽  
Test Concentration ◽  
And Staphylococcus Aureus ◽  
One Way Anova

Kerehau (Callicarpa longifolia Lam) is a plant that has traditionally efficacious as a cure colds and diarrhea. Part used as medicine are the leaves. This study aims to determine the antibacterial activity of ethanol extract of leaves kerehau on Escherichia coli and Staphylococcus aureus. Kerehau leaf extract prepared by maceration method using ethanol 70%, with a test concentration of 10%, 25%, 50%, and 100% and chloramphenicol 0.1% (w / v) as a positive control and dimethyl sulfoxide 1% (v / v) as a negative control. The results of antibacterial activity test with extract concentrations of 25%, 50% and 100% in Escherichia coli is 9.45 mm, 12.21 mm and 17.38 mm, for the positive control is 21.85 mm. The results of antibacterial activity test with extract concentrations of 10%, 25%, 50% and 100% in Staphylococcus aureus was 7.3 mm, 9.85 mm, 13.65 mm and 20.58 mm, for the positive control was 24.05 mm. The antibacterial activity was analyzed by One Way ANOVA showed significant differences between treatments

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 246
Author(s):  
Yayuk Bulam Sarifati ◽  
Sjarif Ismail ◽  
Khemasili Kosala
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Measurement ◽  
Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of ​​inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values ​​<0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

scholarly journals Effectiveness of Ethanolic Extract of Aloe Vera Leaves against Staphylococcus aureus

2020 ◽  
Vol 3 (2) ◽  
pp. 36-40
Author(s):  
Viki Ayu Intan Permatasari ◽  
Mutia Hariani Nurjanah ◽  
Wimbuh Tri Widodo
Keyword(s):  
Staphylococcus Aureus ◽  
Aloe Vera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Negative Control ◽  
Traditional Medicines ◽  
Alternative Ingredients ◽  
High Concentrations

Since long ago Indonesia used nutritious plants as traditional medicines. Various types of plants in Indonesia can be used as alternative ingredients, one of which is aloe vera. Aloe vera contains saponin and anthraquinone, so aloe vera leaves function as antiseptic and antibacteria. Staphylococcus aureus is a gram-positive coccus bacteria. This bacterium is often found as a normal germ flora in humans. Staphylococcus aureus can cause infections in humans and animals. This study aims to determine the effect of ethanolic extract of Aloe vera leaves in inhibiting Staphylococcus aureus by using maceration extract method. The concentrations used were 20%, 40%, 60%, 80% and 100% with positive control (Erytromycin) and negative control (aquades). The inhibitory zone analysis is done using the table method. Test of ethanol extract of Aloe vera leaves in inhibiting Staphylococcus aureus produced inhibition zones at concentrations of 60%, 80% and 100% with average diameter of 6.94 mm, 6.22 mm and 9.5 mm. The conclusion of this research is the ethanolic extract of Aloe vera leaves can inhibit Staphylococcus aureus in high concentrations

scholarly journals Antiinflammatory Activity of Pagoda Flower (Clerodendrum Paniculatum L.) Ethanol Extract Using Paw Edema Method

2019 ◽  
Vol 7 (6) ◽  
pp. 43-45
Author(s):  
Ihsanul Hafiz ◽  
Mandike Ginting
Keyword(s):  
Antiinflammatory Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Paw Edema ◽  
Laboratory Design ◽  
White Rats ◽  
Experimental Laboratory ◽  
The Difference

Objectives: The purpose of this study was to determine antiinflammatory activity of pagoda flower (Clerodendrum paniculatum L.) ethanolic extract Design: This study uses an experimental laboratory design. This research uses paw edema method by inducing carrageenin in the legs of male white rats as an induction of inflammation. Interventions: The sample used was pagoda flower ethanol extract in various dosages of 25, 50 and 100 mg / kg. As a comparison, acetosal dose 33 mg / kg was used. Na CMC suspension was used as a negative control. Main outcome measure: The results in this study are the difference in the volume of edema volume from rat feet per unit time. The measurement of the rat's leg volume was measured at 30, 60, 120, 180, 240 and 300 minutes. Conclusion: Pagoda flower ethanol extract does not have good anti-inflammatory activity. there were no significant differences between groups except at dose 100 and positive control at minute 300 of negative control.    

scholarly journals COMPARISON OF α –GLUCOSIDASE INHIBITORY ACTIVITY OF Moringa oleifera ETHANOLIC EXTRACT

2021 ◽  
Vol 9 (Spl-2-ICOPMES_2020) ◽  
pp. S269-S273
Author(s):  
Rizky Rahmwaty Alami ◽  
◽  
Herlina Rante ◽  
Yulia Yusrini Djabir ◽  
◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Moringa Oleifera ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Glucosidase Inhibitor ◽  
Plant Samples

The purpose of this research was to determine the α-glucosidase enzyme inhibitory activity of Moringa oleifera plant samples collected from the three geographical areas viz., Saragi, Bacuhau, and Batumatongka of Southeast Sulawesi Indonesia. Ethanol extract of Moringa leaves was prepared by the maceration method using 95% ethanol. The estimation of α –glucosidase inhibitory activity of this extract was performed in vitro. The results of the study showed that ethanolic extract of three Moringa samples i.e. Sarangi, Bacuhau, and Batumatongka had the IC50value of 18.62, 10.18, 10.58 ppm, respectively while IC50value for the acarbose positive control was reported 11.54ppm. From the results of this study, it can be concluded that ethanolic extract of Moringa could inhibit α –glucosidase and this potential was similar to the commercial α –glucosidase inhibitor acarbose.

scholarly journals Antibacterial activities of combination of ethanol extract of Delima fruit (Punica granatum L.) and Sereh stem (Cymbopogon citratus) on Staphylococcus aureus

2019 ◽  
Vol 6 (12) ◽  
pp. 5037
Author(s):  
Melia Pebrina ◽  
Indah Komala Sari ◽  
Eliza Arman ◽  
Honesty Diana Morika
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Antibacterial Activities ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Pomegranate Peel Extract

ABSTRACT Background: Pomegranates contain chemical saponins and flavonoids while lemongrass stems have flavonoid chemical compounds. Substances that can inhibit the linking of bacteria are saponins and flavonoids. The most common microorganism found in ulcus diabetikum (ulcer diabetes) is Staphylococcus aureus. The purpose of this study was to determine the antibacterial activity of a combination of pomegranate ethanol extract and ethanol extract of lemongrass stems against S. aureus bacteria.Methods: This study used an experimental design with a complete randomized design study divided into 5 groups: groups 1 (75:25), 2 (50:50), 3 (25:75), positive control (tetracycline) and negative control. Manufacture of pomegranate peel extract and lemongrass stems was done by maceration method for further rotary, after the extract was obtained tested for antibacterial activity by diffusion method using a cylinder. With the test used analysis of variance one way.Results: The results of this study showed that the combination of pomegranate ethanol extract and citronella stem ethanol extract showed activity against S. aureus with a ratio of 15 ul:5 ul, 15 ul:15 ul, 5 ul:15 ul with inhibitory diameter of  respectively 13 mm, 11 mm, 8 mm. Whereas for positive control with chloramphenicol, the inhibition area is 9 mm.Conclusions: Statistically the combination of pomegranate ethanol extract and lemongrass stems has antibacterial power which uses p=0.005 and p<0.05. The combination of pomegranate ethanol extract and lemongrass stems has very strong antibacterial activity against S. aureus due to inhibition zones of 10-20 mm.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 114
Author(s):  
Lyone Katiandagho ◽  
Defny S Wewengkang ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Soft Coral ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Water Fraction

ABSTRACT Soft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine  organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial. ABSTRAK Karang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN BAWANG DAYAK (Eleutherine palmifolia (L.) Merr) TERHADAP PERTUMBUHAN Propionibacterium acne

2020 ◽  
Vol 3 (2) ◽  
pp. 261-270
Author(s):  
Eka Kumalasari ◽  
◽  
aina aina ◽  
noverda ayu checaria ◽  
noor aisyah
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antibacterial Compounds ◽  
Propionibacterium Acne ◽  
Facial Changes

Acne that appears can cause facial changes, in the form of swelling, redness, purulence and causing pain so that it will create an impression that is less attractive in appearance and affects one's beauty. One of the causes of acne is Propionibacterium acne. Dayak onion leaves are one of the plant that contain antibacterial compounds. The purpose of this study was to determine the antibacterial activity of ethanol extract of onion leaves on the growth of Propionibacterium acne.The antibacterial activity of this plant needs to be reviewed through with extract group with various concentrations, positive control group (clindamycin 0.1%) and negative control group (Aquadest). The results showed that the ethanol extract of dayak onion leaves had antibacterial activity against the growth of Propionibacterium acne. The diameter of the inhibition zone formed at concentrations of 20%, 40%, 60%, 80% and 100% respectively 7.25 mm (medium), 10.43 mm (strong), 15.23 mm (strong), 18, 55 mm (strong) and 22.20 mm (very strong).

scholarly journals Assessment of Nephroprotective Potential of Ethanolic Extract Premna Tomentosa (Ept) in Alcohol Toxicity in Male Albino Rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3099-3103
Author(s):  
Gomathi Priyadharsini T ◽  
Kavimani M ◽  
Sathiya Narayana Murthy S ◽  
Prabhu K ◽  
Arudyuti Chowdhury
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Blood Parameters ◽  
Positive Control ◽  
Negative Control ◽  
Albino Rats ◽  
Phytochemical Analysis ◽  
Histopathological Changes

The nephroprotective prospective of Premna tomentosa extract against Alcohol induced nephrotoxicity in rats was investigated in the present study. The characterization of ethanol extract of Premna tomentosa (EPT) was performed using standard phytochemical analysis. Male albino wistar rats 36 in numbers were divided into 6 groups including control, negative control, positive control and various doses of EPT Treated groups; Nephrotoxicity was induced by alcohol (1ml/100gm b.wt) in animals. Rats intoxicated with Alcohol were fed with 500, 750 mg/kg dose of EPT and Liv 52 (1ml/100gm b.wt) for 60 days. Results show that EPT (500mg/kg b.wt) had a significant effect against alcohol induced nephrotoxicity in rats than EPT (750mg/kg b.wt) in blood parameters and consonantly good histopathological changes in kidney. The deleterious histopathological alterations in kidney associated with glomerular and tubular changes in alcohol intoxicated rats was evident. This result shows Premna tomentosa may be used as supplementary drug for alcoholics.

scholarly journals Sambiloto (Andrographis paniculata Nees.) leaf extract activity as an α-Amylase enzyme inhibitor

2021 ◽  
pp. 305-308
Author(s):  
Yustina Sri Hartini ◽  
Dewi Setyaningsih ◽  
Maria Josephine Vivian Chang ◽  
Maria Cyrilla Iglesia Adi Nugrahanti
Keyword(s):  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Enzyme Inhibitor ◽  
Statistical Tests ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Andrographis Paniculata ◽  
Activity Test ◽  
Ic50 Value

Introduction: Sambiloto (Andrographis paniculata) is an antidiabetic medicinal plant that acts by inhibiting the α-amylase enzyme. Andrographolide, the active compound of sambiloto leaf, is insoluble in water but dissolves in ethanol. Aim: This study compared the in vitro activity of aqueous extract and ethanolic extract of sambiloto leaf with the α-amylase enzyme. Methods: The inhibitory activity test of the α-amylase enzyme was carried out using the ultraviolet-visible spectrophotometric method by measuring the absorbance of the remaining starch, which forms a blue complex with iodine-iodide. Results: The inhibitory activity of the α-amylase enzyme of the aqueous extract of sambiloto leaf (with the IC50 value of 14.203 ± 0.112 mg/mL) was lower than that of the ethanol extract (with the IC50 value of 9.253 ± 0.116 mg/mL). The results of the statistical tests showed significant differences (p <0.05) between the inhibitory activity of the α-amylase enzyme acarbose and the activity of both extracts.

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