scholarly journals Aktivitas Antioksidan dan Screening Fitokimia Kulit Bligo (Benincasa hispida)

2021 ◽  
Vol 17 (1) ◽  
pp. 27
Author(s):  
Dini Nur Hakiki ◽  
Athiefah Fauziyyah ◽  
Sri Wijanarti
Keyword(s):  
Antioxidant Activity ◽  
Health Effects ◽  
Screening Test ◽  
Uronic Acid ◽  
Phytochemical Screening ◽  
Active Compounds ◽  
Activity Test ◽  
Benincasa Hispida ◽  
Anti Inflammatory

<p>Bligo (<em>Benincasa hispida</em>) merupakan tanaman yang tumbuh di daerah tropis dan subtropis. Buah bligo mengandung senyawa-senyawa aktif antara lain flavonoid, glikosida, karoten, vitamin, mineral, dan asam uronat. Penelitian di bidang farmakologi telah mengungkapkan bahwa buah bligo memberikan efek kesehatan, antara lain <em>antidepressant</em>, ansiolitik, antioksidan, antiinflamatori, diuretik, antidiabetik, dan antimikrobia. Namun penelitian di Indonesia mengenai kulit bligo masih sangat terbatas. Penelitian ini bertujuan untuk melakukan aktivitas antioksidan dan <em>screening</em> fitokimia pada kulit bligo. Kulit bligo dikeringkan kemudian digiling menjadi bubuk ukuran 100 mesh. Pengujian yang dilakukan adalah uji aktivitas antioksidan menggunakan DPPH, dan uji <em>screening</em> fitokimia kualitatif menggunakan LC-MS/MS QTOF. Aktivitas antioksidan pada kulit bligo adalah kelas medium dengan nilai IC<sub>50</sub> 6,91 mg/mL. Dari hasil analisis LC-MS/MS QTOF diketahui bahwa kulit bligo mengandung 36 senyawa, dengan 5 senyawa dominannya adalah adenosin; polidatin; 7-(4-hidroksi-3-metoksifenil)-l-(4-hidroksifenil)-4E,6E-heptadien-3-on; morasin C; dan kushenol S. Hasil karakterisasi yang diperoleh menunjukkan bahwa kulit bligo berpotensi sebagai senyawa aktif.</p><p><strong></strong><strong>Antioxidant Activity and Phytochemical Screening of Peel Bligo (<em>Benincasa hispida</em>). </strong>Bligo (<em>Benincasa hispida</em>) is a plant that grows in tropical and subtropical regions. Bligo fruit contains active compounds such as flavonoids, glycosides, carotene, vitamins, minerals, and uronic acid. Research in the field of pharmacology has revealed that bligo fruit has health effects, including antidepressants, anxiolytics, antioxidants, anti-inflammatory, diuretics, antidiabetic, and antimicrobials. However, research on bligo peel is very limited. This study aims were to carry out antioxidant activity and phytochemical screening of bligo peel characteristics. Bligo’s peel was dried and ground into 100 mesh powder. Then antioxidant activity test using DPPH, and qualitative phytochemical screening test using LC-MS/MS QTOF were conducted. The bligo peel is medium class of antioxidant activity with IC<sub>50</sub> value was 6.91 mg/mL. From the results of LC-MS/MS QTOF analysis it was known that bligo skin contained 36 compounds, with 5 dominant compounds, namely adenosine; polydatin; 7-(4-hydroxy-3-methoxyphenyl)-1-(4-hydroxyphenyl)-4E, 6E-heptadien-3-one; moracin C; and kushenol S. The results of the characterization showed that bligo skin has the potential as a source of active compounds for further utilization.</p>

Antioxidant Activity, Inhibition α-Glucosidase of Ethanol Extract of Strychnos nitida G. Don and Identification of Active Compounds

2019 ◽  
Vol 5 (3) ◽  
pp. 11-20
Author(s):  
Stefani Dhale Rale ◽  
Hasim Hasim ◽  
Syamsul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Inhibition Activity ◽  
Active Compounds ◽  
Activity Inhibition ◽  
Activity Test ◽  
Ic50 Value ◽  
Treatment Of Diabetes

This study aims to find the treatment of diabetes using natural materials by exploring plants in the province of East Nusa Tenggara. his research was conducted out by extracting the Strychnos nitida G.Don stem using a method of maceration by ethanol 70%. Ethanol extract was then fractionated using n-hexane and ethyl acetate. Simplicia from maceration and fractionation results were then tested for antioxidant activity, α-glucosidase inhibition activity and identification of active compounds. The results showed that ethyl acetate fraction had the lowest IC50 value of 86.83 μg / ml. Results of the α-glucosidase activity test showed that ethyl acetate fraction and n-heksan fraction at 900 ppm had the highest percentage of inhibition of 34.23% and 33.89%. Identification using LCMS/MS method showed that ethyl acetate fraction consist of Benzenemethamine, N, N-dioctyl- as an antioxidantcompound and compound 24-methyl-5-cholestone-hexol as an antidiabetic compound. From the results of this study, we concluded that the extract of kayu ular Strychnos nitida G.Don stem has inhibition activity toward α-glucosidase enzyme.

scholarly journals Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi) sebagai Antioksidan dan Antiinflamasi

2019 ◽  
Vol 8 (3) ◽  
pp. 86
Author(s):  
Hasim Hasim ◽  
Yupi Yulianita Arifin ◽  
Dimas Andrianto ◽  
Didah Nur Faridah
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Phenolic And Flavonoid

Belimbing wuluh merupakan tanaman jenis buah dan obat tradisional. Tanaman belimbing wuluh sudah sering dimanfaatkan masyarakat salah satunya untuk mengobati penyakit seperti batuk dan radang rektum. Tujuan penelitian ini adalah menguji senyawa fitokimia, menganalisis kandungan total fenolik dan flavonoid, serta aktivitas antioksidan dan antiinflamasi secara in vitro pada ekstrak etanol daun belimbing wuluh. Metode yang dilakukan pada penelitian ini adalah ekstraksi daun belimbing wuluh, skrining fitokimia, perhitungan total fenol dan flavonoid, uji aktivitas antioksidan, dan uji aktivitas antiinflamasi. Senyawa fitokimia yang terkandung pada ekstrak etanol daun belimbing wuluh adalah saponin, tanin, steroid, flavonoid, dan alkaloid. Kandungan total fenolik dan flavonoid ekstrak etanol daun belimbing wuluh secara berturut-turut sebesar 39,03 dan 97,28 µg QE/mg. Ekstrak etanol daun belimbing wuluh memiliki aktivitas antioksidan yang tergolong sangat kuat, sementara aktivitas antiinflamasinya terutama ditunjukkan pada konsentrasi ekstrak 200 µg/ ml, memiliki nilai persen inhibisi hemolisis yang paling tinggi. Kesimpulannya, ekstrak etanol daun belimbing wuluh dapat menjadi salah satu sumber antioksidan dan antiinflamasi alami.Ethanol Extracts of Averrhoa Bilimbi Leaf Demonstrated Antioxidative and Anti-inflammatory ActivityAbstractAverrhoa bilimbi, a fruit-bearing plant, has been traditionally used for medicinal purposes such as treatment of cough and rectal inflammation. In this current work, ethanol extract of Averrhoa bilimbi leaf was analyzed, with regard to phytochemical composition, i.e. total phenolic, and flavonoid, antioxidant activity, and in vitro antiinflammation activity. Extraction of carambola leaves, phytochemical screening, total phenolic and flavonoid contens, assay of antioxidant activity, and assay of anti-inflammatory activity were done in this research. As a result, the leaf extract positively contained some phytochemical compounds, i.e. saponin, tannin, steroid, flavonoid, and alkaloid. Furthermore, total phenolic and flavonoid of the leaf extract was found at 39.03 and 97.28 µg QE/mg extract, respectively. Additionally, antioxidant activity of the leaf extract was classified as very strong, while its anti-inflammatory feature at extract concentration of 200 µg/ml exhibited the highest inhibition of hemolysis. In summary, the ability of Averrhoa bilimbi leaf ethanol extracts to act as antioxidative and anti-inflammatory agents was determined and this may open the use for natural antioxidant and anti-inflammatory agents.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SENYAWA PIRAZOLIN 3-(2- METOKSI-FENIL)-5-NAFTALEN-1-IL-4,5-DIHIDRO-1H-PIRAZOL

2018 ◽  
Vol 8 (2) ◽  
pp. 53-58
Author(s):  
Nurlaili ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Microwave Irradiation ◽  
Biological Activities ◽  
Spectroscopy Data ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Inflammatory

Pyrazoline compounds are reported to have many useful biological activities such as antimicrobials, antihyperglycemia, antioxidants, anti-inflammatory, anticancer and anticonvulsants. The pyrazoline analogues of 3- (2-methoxy-phenyl) -5-naphthalene-1-yl-4,5-dihydro-1H-pyrazol were synthesized usingglacial acetic acid as catalyst and assisted by microwave irradiation. The structures of the compounds were obtainedby UV, IR and HRMS spectroscopy data. Antioxidant activity test using DPPH method showed that pyrazoline compound had IC50 value of 27.41 μg/mL.

scholarly journals Phytochemical Screening and In Vitro Antioxidant and Anti-inflammatory Activities of Aerial Parts of Euphorbia hirta L.

2021 ◽  
Vol 42 (1) ◽  
pp. 115-124
Author(s):  
Deepak Basyal ◽  
Astha Neupane ◽  
Durga Prasad Pandey ◽  
Shiva Pandeya
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Euphorbia Hirta ◽  
Anti Inflammatory

Euphorbia hirta L (Euphorbiaceae) also called asthma herb has long been prescribed in traditional medicine because it exhibits diverse pharmacological actions due to the presence of alkaloids, flavonoids, polyphenols, triterpenoids, and saponins. The present study is aimed at the study of phytochemical and antioxidant activity and anti-inflammatory screening of E. hirta. Extraction of dried powder was performed followed by phytochemical screening using color reactions. Total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were estimated by Folin-Ciocalteu and Aluminum chloride method respectively. The antioxidant activity was studied by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method.  Anti-inflammatory activity was studied by using protein denaturation in vitro bioassay. Phytochemical screening showed the presence of flavonoids, alkaloids, and phenolic compounds. TPC, TFC and antioxidant activity (IC50) of the extract were found as 288.10 mg gallic acid equivalent per gram (GAE/g), 29.36 mg quercetin equivalent per gram (QE/g) and 32.23 µg/mL (p<0.05) respectively. Diclofenac sodium and E. hirta extract showed the maximum inhibition of 91.28% and 68.20% respectively at the concentration of 1000 µg/mL compared with control (p>0.05). The phenolic compounds and flavonoids exert antioxidant and anti-inflammatory activities because of their scavenging ability. The demonstrated antioxidant and anti-inflammatory activities may be the rationale behind some of its folkloric uses and also may be responsible for some of its pharmacological effects. Thus, E. hirta can be considered a good source of antioxidants and anti-inflammatory actions, which might be beneficial for combating oxidative stress.

scholarly journals PENENTUAN AKTIVITAS TABIR SURYA DAN ANTIOKSIDAN EKSTRAK ETANOL BENALU (Henslowia frutescens) INANG JERUK BALI SECARA IN VITRO

2021 ◽  
Vol 6 (1) ◽  
pp. 124-132
Author(s):  
Rusdiati Helmidanora ◽  
◽  
Henny Nurhasnawati ◽  
Yullia Sukawaty ◽  
Andri Priyoherianto ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Yield ◽  
Ethanol Extract ◽  
Parasitic Plant ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Measurement Results ◽  
High Antioxidant Activity ◽  
Yield Calculation

Henslowia frutescens is a semi-parasitic plant, although considered harmful, it has medicinal and cosmetic potential. This plant has secondary metabolite compounds, namely phenolic compounds, flavonoids, saponins, tannins, and steroids which have high antioxidant activity. The purpose of this study was to determine the sunscreen activity of the ethanol extract of Henslowia frutescens based on the in vitro SPF (Sun Protecting Factor) value and to determine its antioxidant activity. The research stages included sampling, sampling, maceration extraction with 95% ethanol, yield calculation, phytochemical screening, sunscreen activity test based on SPF value, and antioxidant activity test. Data analysis was carried out descriptively based on the measurement results processed according to the calculation formula. The results showed that the sunscreen activity of the ethanol extract of Henslowia frutescens based on the in vitro SPF value was 14.57 - 25.20 with moderate protection category. The antioxidant activity of the ethanol extract of Henslowia frutescens showed a very strong category with an IC value of 22.82 ± 1.33.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DAUN BENALU KOPI (Loranthus Ferrugineus Roxb.) DENGAN METODE DPPH (1,1 – Difenil -2- Pikrilhidrazil)

2019 ◽  
Vol 6 (2) ◽  
pp. 192
Author(s):  
Muammar Yulian ◽  
Safrijal Safrijal
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Secondary Metabolites ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Dpph Method ◽  
Ic50 Values ◽  
X 24

The study about antioxidant activity test of coffee parasite leaves (Loranthus ferrugineus Roxb.) by 1,1-diphenyl-2-pikrilhidrazyl (DPPH) method has been done. The aim of this study was to determine the content and activity of secondary metabolites, flavonoids and antioxidant, which found in the coffee parasite leaves. Dry powder of parasite coffee leaves (Loranthus ferrugineus Roxb.) as much as 0.5 kg were macerated by 2 L of ethanol solvent at room temperature for 4 x 24 hours, then mixed and filtered. Ethanol filtrate was evaporated at 30-40°C by using a rotary evaporator to obtain the crude extract of coffee parasite leaves. The results of the phytochemical screening showed positively that the extract was containing alkaloid, flavonoids, saponins, tannins and steroid compounds.The results of the antioxidant activity test by using DPPH method was obtained that the amount of antioxidant activity of the samples of ethanol extract had a very strong antioxidant activity against radical DPPH 0.05 mM, with IC50 values was obtained 6.063 ppm. Whereas, for comparison of ascorbic acid was about 3.127 ppm.

scholarly journals Skrining Fitokimia dan Uji Aktivitas Antioksidan dari Daun Nasi (Phrynium capitatum) dengan Metode DPPH (1,1-difenil-2-pikrilhidrazil)

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (1) ◽  
pp. 11 ◽  
Author(s):  
Dwi Putra Wijaya ◽  
Jessy E. Paendong ◽  
Jemmy Abidjulu
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Activity Test ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dpph Solution

Telah dilakukan penelitian tentang skrining fitokimia dan uji aktivitas antioksidan dari daun nasi (Phrynium capitatum) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Skrining fitokimia meliputi pengujian alkaloid, flavonoid, saponin, steroid, triterpenoid dan fenolik. Uji aktivitas antioksidan dilakukan dengan penambahan 2 mL larutan DPPH dengan 0,5 mL larutan ekstrak dengan konsentrasi masing-masing 1000, 750, 500 dan 250 µg/mL. Data yang diproleh kemudian digunakan untuk menghitung nilai IC50 dari ekstrak etanol daun nasi. Hasil pengujian menunjukan bahwa ekstrak etanol daun nasi mengandung senyawa alkaloid, flavonoid, saponin, steroid dan fenolik. Ekstrak etanol daun nasi memiliki aktivitas antioksidan trrtinggi pada konsentrasi 1000 µg/mL sebesar 65,77 % dan semakin menurun aktivitasnya dengan berkurangnya konsentrasi ekstrak, konsentrasi 750, 500 dan 250 µg/mL masing-masing sebesar 53,96 %, 43,8 % dan 19,58 % dengan nilai IC50 pada konsentrasi 701,78 µg/mL.A research on phytochemicals screening and antioxidant activity test of daun nasi (Phrynium capitatum) using DPPH (1,1-diphenyl-2-picrylhydrazyl) method had been conducted. Test for alkaloids, flavonoids, saponins, steroids, triterpenoids and phenolic were included in phytochemical screening. Antioxidant activity was tested through the addition of 2 mL DPPH solution into 0.5 mL of the extract having a concentration of 1000, 750, 500 and 250 µg/mL respectively. The data obtained was used to calculate the IC50 value for the ethanol extract of daun nasi. The results showed that the ethanol extract of daun nasi contains alkaloids, flavonoids, saponins, steroids and phenolic. Ethanol extract of daun nasi has the highest antioxidant activity of 65.77% at concentration of 1000 µg/mL. Its activity decreased with decreasing concentration of the extract, which were 53.96 %, 43.8 % and 19.58 % at concentration of 750, 500 and 250 µg/mL respectively. IC50 values was found at a concentration of 701.78 µg/mL.

scholarly journals Uji Aktivitas Antioksidan Kombinasi Ekstrak Umbi Bawang Tiwai (Eleutherine bulbosa (Mill) Urb) dan Rimpang Kunyit(Curcuma domestica Val.)

2020 ◽  
Vol 10 ◽  
pp. 151-155
Author(s):  
Yoni Miftahun Nafsiyah Agustin ◽  
Lisna Meylina ◽  
Yurika Sastyarina
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plant ◽  
Bioactive Compounds ◽  
Sample Solution ◽  
Activity Test ◽  
Measured Absorption ◽  
Anti Inflammatory

Bawang tiwai (Eleutherine bulbosa (Mill) Urb)a plant that originated from Kalimantan. This plant is traditionally used as a medicinal plant in the community. Bawang tiwai have bioactive compounds such as alkaloids, flavonoids, saponins which act as antioxidants. In addition bawang tiwai, turmeric (Curcuma domestica Val.)is a traditional medicinal plant used for seasoning and is a medicinal plant as antimicrobial, antioxidant, anti-tumor and anti-inflammatory. The aim of the research is to measure the antioxidant activity of the combination of extracts of bawang tiwai(Eleutherine bulbosa (Mill) Urb)and Turmeric (Curcuma domestica Val.). Antioxidant activity test used the DPPH (1,1-Diphenyl-2-picryl Hidrazil) measured absorption at a wavelength of 517 nm. Antioxidant activity was assessed based on% inhibition (IC50) which is the concentration of the sample solution is needed to reduce free radicals by 50%. The results obtained by measuring the antioxidant activity fromcombination extracts of IC50 was 185,118 ppm respectively.

scholarly journals Potensi Ekstrak Teripang Stichopus hermanii, Semper 1868 (Holothuroidea : Stichopodidae) sebagai Penghasil Senyawa Antibakteri terhadap Streptococcus mutans Clarke, 1924 (Bacilli : Streptococcaceae)

2021 ◽  
Vol 10 (3) ◽  
pp. 421-427
Author(s):  
Rika Monika ◽  
Delianis Pringgenies ◽  
Wilis Ari Setyati
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Sea Cucumber ◽  
Diffusion Method ◽  
Marine Biota ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Active Compounds ◽  
Activity Test

Teripang Stichopus hermanii merupakan biota laut yang mempunyai senyawa dengan bioaktivitas sebagai antibakteri terhadap patogen. Stichopus hermanii mampu menghambat bakteri gram positif dan negatif. Produk teripang yang sudah ada banyak memperlihatkan manfaatnya salah satunya untuk gigi. Produk komersil yang terlihat di masyarakat yakni pasta gigi, namun belum diketehaui  secara mendetail mengenai seberapa besar peran pasta gigi tersebut dalam mengatasi permasalahn gigi.  Tujuan dari penelitian ini untuk mengetahui senyawa yang aktif didalam ekstrak teripang Stichopus hermanii. Metode ekstraksi yakni dengan padat ke cair, pencarian senyawa aktif menggunakan skrining fitokimia dengan pereaksi yang berbeda setiap pengujiannya, dan uji aktivitas antibakteri menggunakan metode difusi agar dengan konsentrasi yang berbeda-beda. Uji aktivitas antibakteri pada ekstrak S. hermanii menggunakan bakteri patogen Streptococcus mutans. Hasil senyawa aktif yang didapat dari skrinining fitokimia meliputi flavonoid, alkaloid, triterpenoid dan saponin. Uji aktivitas antibakteri menunjukkan bahwa ekstrak metanol S. hermanii mampu menghambat bakteri S.mutans. Zona hambat tertinggi pada S. mutans  5,86 mm ± 4,92 dengan konsentrasi 80 µg/disk pada waktu 24 jam. Disimpulkan bahwa senyawa aktif pada ekstrak metanol S. hermanii mempunyai bioaktivitas antibakteri pada bakteri S. mutans.  The sea cucumber Stichopus hermanii is a marine biota that has compounds with bioactivity as an antibacterial against pathogens. Stichopus hermanii can inhibit gram-positive and gram-negative bacteria. Sea cucumber products that already exist have shown many benefits, one of which is for teeth. The commercial product seen in the community is toothpaste, but it is not yet known in detail how big the role of toothpaste is in overcoming dental problems. The purpose of this study was to determine the active compounds in sea cucumber extract Stichopus hermanii. The extraction method is solid to liquid, the search for active compounds uses phytochemical screening with different reagents for each test, and the antibacterial activity test uses the agar diffusion method with different concentrations. Antibacterial activity test on S. hermanii extract using the pathogenic bacterium Streptococcus mutans. The results of the active compounds obtained from phytochemical screening include flavonoids, alkaloids, triterpenoids, and saponins. The antibacterial activity test showed that the methanol extract of S. hermanii was able to inhibit S. mutans bacteria. The highest zone of inhibition in S. mutans was 5.86 mm ± 4.92 with a concentration of 80 g/disk at 24 hours. It was concluded that the active compound in the methanolic extract of S. hermanii had antibacterial bioactivity on S. mutans bacteria.

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