scholarly journals Antioxidant Activity of Methanol Extract of the Purun Tikus (Eleocharis dulcis) Leaves

2018 ◽  
Vol 3 (3) ◽  
pp. 135 ◽  
Author(s):  
Kholifatu Rosyidah ◽  
Taufiqur Rohman ◽  
Ridhana Fitriani
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Methanol Extract ◽  
Open Water ◽  
Extreme Conditions ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Dpph Method ◽  
Acidic Sulfate

<p>Purun tikus (<em>Eleocharis dulcis</em>) is a kind of swamp plant, can be found in open water areas, both in swamps and rice fields. <em>Purun tikus</em> is an indicator vegetation of acidic sulfate soil. Chemically, plants that able to adapt to special or extreme conditions, usually produce a compound that are unique and have unique bioactivity as well. This paper reports a phytochemical screening of <em>Purun tikus</em> methanol extract and its antioxidant activity. The results showed that the methanol extract of <em>Purun tikus</em> leaves contained phenolic compounds, flavonoids, tannins and terpenoids. By using DPPH method as an antioxidant test, the methanol extract of purun tikus leaves showed a strong activity, with IC<sub>50</sub> value was 58.58 ppm while vitamin C had an IC<sub>50</sub> value of 4.50 ppm.</p>

scholarly journals Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method

2021 ◽  
Vol 7 (2) ◽  
pp. 275
Author(s):  
Dewa Ayu Puspaning Kumaradewi ◽  
Windah Anugrah Subaidah ◽  
Yayuk Andayani ◽  
Ali Al-Mokaram
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Color Test ◽  
Activity Test ◽  
Dpph Method

Research on phytochemical screening and antioxidant activity testing of the ethanol extract of buni leaves (Antidesma bunius L. Spreng) has been carried out. This study aims to identify secondary metabolite compounds contained in buni leaf plants to see the activity of the ethanol extract of buni leaves using the DPPH method. Buni leaves were extracted in 96% ethanol by maceration method, then evaporated using a rotary evaporator. The results of thick extract were screened for phytochemicals such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids using a qualitative color test method and carrying out antioxidant activity using a UV-Vis spectrophotometer with Vitamin C as a comparison. The results showed that the ethanol extract of buni leaves contained phenolic compounds, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as IC50 Vitamin C of 5.22 ppm which is classified as a very strong antioxidant, while the IC50 of buni leaves ethanol extract is lower at 61, 8 ppm which is classified as a strong antioxidant

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT BATANG MAHONI (Swietenia mahagoni Jacq.) MENGGUNAKAN METODE DPPH (1,1 diphenyl-2-picrylhydrazyl)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 548
Author(s):  
Amanda T. M. Turangan ◽  
Defny S. Wewengkang ◽  
Adithya Yudistira
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Medicinal Plant ◽  
Positive Control ◽  
Antioxidant Compounds ◽  
Phytochemical Screening ◽  
Oxidation Reactions ◽  
Dpph Method ◽  
Swietenia Mahagoni

Antioxidant are compounds that are able to inactivate the development of the oxidation reactions of the molecules or neutralize free radicals. Mahogany bark (Swietenia mahagoni Jacq.) has properties as a medicinal plant. Through the phytochemical screening, the mahogany bark (Swietenia mahagoni Jacq.) contains antioxidant compounds such as alkaloids, saponins and phenolics. The aim of this study was to determine the presence of antioxsidant activity in the extract of mahogany bark (Swietenia mahagoni Jacq.). using the DPPH method with a concentration of 250 mg / L, 200 mg / L, 150 mg / L and 100 mg / L and Vitamin C p.a as a positive control. Each sample made three repetitions of the test. The test uses a UV-Vis Spectrophotometer. The result of the study showed that mahogany bark (Swietenia mahagoni Jacq.) showed antioxidant activity with the percentage of 21,50% at the concentration at 250 mg / LKeywords : Swietenia mahagoni Jacq., Antioxidant, Ethanol, DPPHABSTRAKAntioksidan ialah senyawa yang mampu menginaktivasi berkembangnya reaksi oksidasi molekul lain atau menetralisir radikal bebas. Kulit Batang Mahoni (Swietenia mahagoni Jacq.) memiliki khasiat sebagai tanaman obat. Melalui skrining fitokimia, Swietenia mahagoni Jacq. Mengandung senyawa antioksidan seperti alkaloid, saponin dan fenolik. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan didalam ekstrak tanaman Kulit Batang Switenia mahagoni Jacq. menggunakan metode DPPH dengan konsentrasi 250 mg/L, 200 mg/L, 150 mg/L dan 100 mg/L dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat Spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ekstrak Kulit Batang Swietenia mahagoni Jacq. memiliki aktivitas antioksidan dengan presentase sebesar 21,50% pada konsentrasi 250 mg/L Kata Kunci : Swietenia mahagoni Jacq., Antioksidan, Etanol, DPPH

Antioxidant Activity Of Methanol Extract Of Kesum Leaves (Polygonum Minus Huds.) DPPH MethodAktivitas Antioksidan Ekstrak Metanol Daun Kesum (Polygonum Minus Huds.) Metode DPPH

2018 ◽  
Vol 1 (2) ◽  
pp. 161 ◽  
Author(s):  
Indah Purwaningsih ◽  
Reskiya Sapriani ◽  
Ratih Indrawati
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Beta Carotene ◽  
Polar Compounds ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
Polygonum Minus ◽  
The Difference ◽  
X 24

Abstract: Kesum Plants (Polygonum minus) is one of the endemic plants in West Borneo containing phenolic compounds, steroids, flavonoids, alkaloids, tannins, saponins, and beta-carotene. Phenolic, flavonoids and beta-carotene are antioxidant compounds. Most phenolic and flavonoid groups are polar compounds. Therefore, in this research used methanol solvent as representative of polar solvent. The purpose of this research  was to know the difference of antioxidant activity of methanol extract of kesum leaves with vitamin C. The extraction  is done by maseration for 3 x 24 hours, where the solvent is changed every 24 hours. The method used to measure antioxidant activity is the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The samples used in this paper are methanol extract of  kesum leaves in 80, 65, 50, 35, and 20 ppm with five replications. The comparator used is vitamin C. The result of the research showed that IC50 of methanol extract was 20,632 ppm and IC50 of vitamin C 6,175 ppm. Based on the results of this study it can be concluded that vitamin C and methanol extract of serum leaves have a very strong antioxidant activity.Abstrak: Tanaman Kesum (Polygonum minus) merupakan salah satu tanaman endemik di wilayah Kalimantan Barat yang mengandung senyawa golongan fenolik, steroid, flavonoid, alkaloid, tanin, saponin, dan betakaroten. Golongan fenolik, flavonoid dan betakaroten merupakan senyawa antioksidan. Sebagian besar golongan fenolik dan flavonoid merupakan senyawa polar. Maka dari itu, pada penelitian ini digunakan pelarut metanol sebagai perwakilan pelarut polar. Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antioksidan ekstrak metanol daun kesum terhadap vitamin C. Proses ekstraksi dilakukan dengan cara maserasi selama 3 x 24 jam, dimana pelarutnya diganti setiap 24 jam sekali. Metode yang digunakan untuk mengukur aktivitas antioksidan adalah metode DPPH (2,2-difenil-1-pikrilhidrazil). Sampel yang digunakan pada penelitian ini yaitu ekstrak metanol daun kesum konsentrasi 80, 65, 50, 35, dan 20 ppm dengan pengulangan sebanyak lima kali. Pembanding yang digunakan adalah vitamin C. Hasil penelitian didapatkan IC50 ekstrak metanol daun kesum sebesar 20.632 ppm dan IC50 vitamin C sebesar 6.175 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa vitamin C dan ekstrak metanol daun kesum memiliki aktivitas antioksidan yang sangat kuat.

scholarly journals Phytochemical Screening and Antioxidant Activity of Date(Phoenix Dactylifera) Seed Extracts

2019 ◽  
Vol 8 (2S7) ◽  
pp. 87-89
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Water Fraction ◽  
Ic50 Value ◽  
Dpph Method

Dates consisting of 90% flesh have been known to have various benefits, but the remaining 10% of them in the form of seeds has not been utilized optimally. The date palm contains a fairly high polyphenol compound, which can act as an antioxidant. This study aims to determine the antioxidant activity of methanol extract and fractionation of the extract of dates using the in vitro method of DPPH (1,1-diphenyl-2-picrilhidrazil). The date seeds are made into simplicia, macerated with methanol, and then the extracted methanol is gradually fractionated by n-hexane, ethyl acetate, n-butanol, and water. Each fraction and extract is phytochemically screened using the standard methods and its antioxidant activity is determined by DPPH method. Methanol extract, ethyl acetate, n-butanol, and water fractions contain tannins, flavonoids, alkaloids, and saponin, while the n-hexane fraction not contain phytochemical compound based on the phytochemical screening. The ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 3.72 ± 0.44 μg/mL, followed by methanol extract of 4.71 ± 0.64 μg/mL, n-butanol fraction of 6.62 ± 0.08 μg/mL, water fraction of 53.00 ± 18.96 μg/mL, and n-hexane fraction of 250.19 ± 9.69 μg/ mL, while the IC50 value of vitamin C as the positive control is 4.29 ± 0.74 μg/mL. Therefore, date seeds can be a source of natural antioxidants

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Biji Langsat (Lansium domesticum var. pubescens) dan Duku (Lansium domesticum var. domesticum) dengan Metode DPPH

2020 ◽  
Vol 20 (2) ◽  
pp. 113
Author(s):  
Josepin P Konda ◽  
Jainer P Siampa ◽  
Trina E Tallei ◽  
Billy J Kepel ◽  
Fatimawali Fatimawali
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Regression Line ◽  
Seed Extract ◽  
Test Method ◽  
Color Test ◽  
Dpph Method ◽  
Lansium Domesticum ◽  
Positive Results

Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas antioksidan ekstrak biji langsat dan duku dengan metode DPPH. Biji buah langsat dan buah duku diekstraksi menggunakan metode maserasi dengan pelarut metanol. Skrining fitokimia yang dilakukan meliputi uji alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin secara kualitatif dengan metode uji warna. Uji aktivitas antioksidan menggunakan metode DPPH dan vitamin C sebagai pembanding, serapan diukur menggunakan spektrofotometer UV-Vis pada panjang gelombang 517 nm. Skrining fitokimia pada ekstrak metanol biji langsat menunjukkan hasil positif terhadap alkaloid, triterpenoid dan saponin, sementara pada ekstrak metanol biji duku menunjukkan hasil positif terhadap alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin. Aktivitas antioksidan ekstrak biji langsat dianalisis menggunakan persamaan garis regresi y = 0,2922x + 33,143, diperoleh IC₅₀ 57,69 μg/mL. Sedangkan aktivitas antioksidan ekstrak biji duku dianalisis menggunakan persamaan garis regresi y = 0.6554x + 29.421, diperoleh IC₅₀ 31,40 μg/mL dan pembanding vitamin C diperoleh IC₅₀ 8,03 μg/mL. Hasil penelitian ini dapat disimpulkan bahwa ekstrak metanol biji buah langsat memiliki kandungan alkaloid, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang kuat sedangkan ekstrak metanol biji buah duku memiliki kandungan alkaloid, flavonoid, tannin, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Antioksidan, biji duku, biji langsat Antioxidant Activity of Methanol Extract of Langsat Seeds (Lansium domesticum var. pubescens) and Duku Seeds (Lansium domesticum var. domesticum)  by Using DPPH Method ABSTRACTThis study aimed to determine the phytochemical content and antioxidant activity of langsat seed extract and duku seed extract by using the DPPH method. Langsat seeds and duku seeds were extracted by using maceration method with methanol as the solvent. Phytochemical screening was carried out including a qualitative test of alkaloids, flavonoids, steroids/triterpenoids, saponins, and tannins by using the color test method. Antioxidant activity was tested by using the DPPH method and vitamin C used as comparison, the absorption was measured by using UV-Vis spectrophotometer at 517 nm wavelength. The result of phytocemical screening on methanol extract of langsat seeds showed positive results on alkaloids, triterpenoids, and saponins, meanwhile methanol extract of duku seeds showed positive results on alkaloids, flavonoids, triterpenoids, tannin and saponins. The antioxidant activity of methanol extract of langsat seeds was analyzed by using the equation of the regression line y = 0.2922x + 33.143, which obtained IC₅₀ value of 57.69 μg/mL. Meanwhile, the antioxidant activity of methanol extract of duku seeds was analyzed by using the equation of the regression line y = 0.6554x + 29.421, obtained IC₅₀ 31,40 μg/mL. Vitamin C obtained IC₅₀ 8.03 μg/mL. On the basis of all results, it can be concluded that the methanol extract of langsat seeds contained alkaloids, saponins and triterpenoid and has a strong antioxidant activity. Meanwhile, the methanol extract of duku seeds contained alkaloids, flavonoids, triterpenoids, tannin and saponins and has a very strong antioxidant activity.Keywords: Antioxidants, duku seeds, langsat seeds

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals SCREENING OF PHYTOCHEMICALS AND QUANTITATIVE ESTIMATION OF TOTAL FLAVONOIDS AND PHENOLIC COMPOUNDS OF LAGASCEA MOLLIS CAV. (ASTERACEAE)

2014 ◽  
Vol 1 (2) ◽  
pp. 94-97
Author(s):  
Saradha M ◽  
Paulsamy S ◽  
Abinaya G
Keyword(s):  
Phenolic Compounds ◽  
Methanol Extract ◽  
Quantitative Estimation ◽  
Root Extract ◽  
Total Flavonoids ◽  
Phytochemical Screening ◽  
Plant Parts ◽  
Soxhlet Apparatus ◽  
Phytochemical Constituents ◽  
Leaves And Roots

Aim of the study was to analyze the phytochemical constituents and estimation of total flavonoids and phenolic compounds of leaves and roots of the medicinal plant Lagascea mollis. Methanol extract of dried leaves and roots of L. mollis was prepared by using soxhlet apparatus. The extract prepared was tested forpreliminary qualitative phytochemical screening, followed by the quantitative estimation of total flavonoids and phenols by spectroscopy. The selected plant parts were found to contain alkaloids, flavonoids and terpenoids in both parts and saponin is present only in leaf. In quantitative estimation the reports revealedthe presence 0.017 and 0.013 mgRE/g extract and 458.91 and 704.50 mgGAE/g extract total flavonoids and phenolic compounds in leaf and root extract respectively. These major phytoconstituents present in this species may be accounted as factors for the medicinal importance of L. mollis.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

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