scholarly journals Aktivitas Antioksidan Ekstrak Metanol Biji Langsat (Lansium domesticum var. pubescens) dan Duku (Lansium domesticum var. domesticum) dengan Metode DPPH

2020 ◽  
Vol 20 (2) ◽  
pp. 113
Author(s):  
Josepin P Konda ◽  
Jainer P Siampa ◽  
Trina E Tallei ◽  
Billy J Kepel ◽  
Fatimawali Fatimawali
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Regression Line ◽  
Seed Extract ◽  
Test Method ◽  
Color Test ◽  
Dpph Method ◽  
Lansium Domesticum ◽  
Positive Results

Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas antioksidan ekstrak biji langsat dan duku dengan metode DPPH. Biji buah langsat dan buah duku diekstraksi menggunakan metode maserasi dengan pelarut metanol. Skrining fitokimia yang dilakukan meliputi uji alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin secara kualitatif dengan metode uji warna. Uji aktivitas antioksidan menggunakan metode DPPH dan vitamin C sebagai pembanding, serapan diukur menggunakan spektrofotometer UV-Vis pada panjang gelombang 517 nm. Skrining fitokimia pada ekstrak metanol biji langsat menunjukkan hasil positif terhadap alkaloid, triterpenoid dan saponin, sementara pada ekstrak metanol biji duku menunjukkan hasil positif terhadap alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin. Aktivitas antioksidan ekstrak biji langsat dianalisis menggunakan persamaan garis regresi y = 0,2922x + 33,143, diperoleh IC₅₀ 57,69 μg/mL. Sedangkan aktivitas antioksidan ekstrak biji duku dianalisis menggunakan persamaan garis regresi y = 0.6554x + 29.421, diperoleh IC₅₀ 31,40 μg/mL dan pembanding vitamin C diperoleh IC₅₀ 8,03 μg/mL. Hasil penelitian ini dapat disimpulkan bahwa ekstrak metanol biji buah langsat memiliki kandungan alkaloid, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang kuat sedangkan ekstrak metanol biji buah duku memiliki kandungan alkaloid, flavonoid, tannin, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Antioksidan, biji duku, biji langsat Antioxidant Activity of Methanol Extract of Langsat Seeds (Lansium domesticum var. pubescens) and Duku Seeds (Lansium domesticum var. domesticum)  by Using DPPH Method ABSTRACTThis study aimed to determine the phytochemical content and antioxidant activity of langsat seed extract and duku seed extract by using the DPPH method. Langsat seeds and duku seeds were extracted by using maceration method with methanol as the solvent. Phytochemical screening was carried out including a qualitative test of alkaloids, flavonoids, steroids/triterpenoids, saponins, and tannins by using the color test method. Antioxidant activity was tested by using the DPPH method and vitamin C used as comparison, the absorption was measured by using UV-Vis spectrophotometer at 517 nm wavelength. The result of phytocemical screening on methanol extract of langsat seeds showed positive results on alkaloids, triterpenoids, and saponins, meanwhile methanol extract of duku seeds showed positive results on alkaloids, flavonoids, triterpenoids, tannin and saponins. The antioxidant activity of methanol extract of langsat seeds was analyzed by using the equation of the regression line y = 0.2922x + 33.143, which obtained IC₅₀ value of 57.69 μg/mL. Meanwhile, the antioxidant activity of methanol extract of duku seeds was analyzed by using the equation of the regression line y = 0.6554x + 29.421, obtained IC₅₀ 31,40 μg/mL. Vitamin C obtained IC₅₀ 8.03 μg/mL. On the basis of all results, it can be concluded that the methanol extract of langsat seeds contained alkaloids, saponins and triterpenoid and has a strong antioxidant activity. Meanwhile, the methanol extract of duku seeds contained alkaloids, flavonoids, triterpenoids, tannin and saponins and has a very strong antioxidant activity.Keywords: Antioxidants, duku seeds, langsat seeds

scholarly journals Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method

2021 ◽  
Vol 7 (2) ◽  
pp. 275
Author(s):  
Dewa Ayu Puspaning Kumaradewi ◽  
Windah Anugrah Subaidah ◽  
Yayuk Andayani ◽  
Ali Al-Mokaram
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Secondary Metabolite ◽  
Ethanol Extract ◽  
Test Method ◽  
Phytochemical Screening ◽  
Color Test ◽  
Activity Test ◽  
Dpph Method

Research on phytochemical screening and antioxidant activity testing of the ethanol extract of buni leaves (Antidesma bunius L. Spreng) has been carried out. This study aims to identify secondary metabolite compounds contained in buni leaf plants to see the activity of the ethanol extract of buni leaves using the DPPH method. Buni leaves were extracted in 96% ethanol by maceration method, then evaporated using a rotary evaporator. The results of thick extract were screened for phytochemicals such as phenolics, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids using a qualitative color test method and carrying out antioxidant activity using a UV-Vis spectrophotometer with Vitamin C as a comparison. The results showed that the ethanol extract of buni leaves contained phenolic compounds, tannins, flavonoids, saponins, alkaloids, steroids and terpenoids as well as IC50 Vitamin C of 5.22 ppm which is classified as a very strong antioxidant, while the IC50 of buni leaves ethanol extract is lower at 61, 8 ppm which is classified as a strong antioxidant

Antioxidant Activity Of Methanol Extract Of Kesum Leaves (Polygonum Minus Huds.) DPPH MethodAktivitas Antioksidan Ekstrak Metanol Daun Kesum (Polygonum Minus Huds.) Metode DPPH

2018 ◽  
Vol 1 (2) ◽  
pp. 161 ◽  
Author(s):  
Indah Purwaningsih ◽  
Reskiya Sapriani ◽  
Ratih Indrawati
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Beta Carotene ◽  
Polar Compounds ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
Polygonum Minus ◽  
The Difference ◽  
X 24

Abstract: Kesum Plants (Polygonum minus) is one of the endemic plants in West Borneo containing phenolic compounds, steroids, flavonoids, alkaloids, tannins, saponins, and beta-carotene. Phenolic, flavonoids and beta-carotene are antioxidant compounds. Most phenolic and flavonoid groups are polar compounds. Therefore, in this research used methanol solvent as representative of polar solvent. The purpose of this research  was to know the difference of antioxidant activity of methanol extract of kesum leaves with vitamin C. The extraction  is done by maseration for 3 x 24 hours, where the solvent is changed every 24 hours. The method used to measure antioxidant activity is the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The samples used in this paper are methanol extract of  kesum leaves in 80, 65, 50, 35, and 20 ppm with five replications. The comparator used is vitamin C. The result of the research showed that IC50 of methanol extract was 20,632 ppm and IC50 of vitamin C 6,175 ppm. Based on the results of this study it can be concluded that vitamin C and methanol extract of serum leaves have a very strong antioxidant activity.Abstrak: Tanaman Kesum (Polygonum minus) merupakan salah satu tanaman endemik di wilayah Kalimantan Barat yang mengandung senyawa golongan fenolik, steroid, flavonoid, alkaloid, tanin, saponin, dan betakaroten. Golongan fenolik, flavonoid dan betakaroten merupakan senyawa antioksidan. Sebagian besar golongan fenolik dan flavonoid merupakan senyawa polar. Maka dari itu, pada penelitian ini digunakan pelarut metanol sebagai perwakilan pelarut polar. Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antioksidan ekstrak metanol daun kesum terhadap vitamin C. Proses ekstraksi dilakukan dengan cara maserasi selama 3 x 24 jam, dimana pelarutnya diganti setiap 24 jam sekali. Metode yang digunakan untuk mengukur aktivitas antioksidan adalah metode DPPH (2,2-difenil-1-pikrilhidrazil). Sampel yang digunakan pada penelitian ini yaitu ekstrak metanol daun kesum konsentrasi 80, 65, 50, 35, dan 20 ppm dengan pengulangan sebanyak lima kali. Pembanding yang digunakan adalah vitamin C. Hasil penelitian didapatkan IC50 ekstrak metanol daun kesum sebesar 20.632 ppm dan IC50 vitamin C sebesar 6.175 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa vitamin C dan ekstrak metanol daun kesum memiliki aktivitas antioksidan yang sangat kuat.

scholarly journals Antioxidant Activity of Methanol Extract of the Purun Tikus (Eleocharis dulcis) Leaves

2018 ◽  
Vol 3 (3) ◽  
pp. 135 ◽  
Author(s):  
Kholifatu Rosyidah ◽  
Taufiqur Rohman ◽  
Ridhana Fitriani
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Methanol Extract ◽  
Open Water ◽  
Extreme Conditions ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Dpph Method ◽  
Acidic Sulfate

<p>Purun tikus (<em>Eleocharis dulcis</em>) is a kind of swamp plant, can be found in open water areas, both in swamps and rice fields. <em>Purun tikus</em> is an indicator vegetation of acidic sulfate soil. Chemically, plants that able to adapt to special or extreme conditions, usually produce a compound that are unique and have unique bioactivity as well. This paper reports a phytochemical screening of <em>Purun tikus</em> methanol extract and its antioxidant activity. The results showed that the methanol extract of <em>Purun tikus</em> leaves contained phenolic compounds, flavonoids, tannins and terpenoids. By using DPPH method as an antioxidant test, the methanol extract of purun tikus leaves showed a strong activity, with IC<sub>50</sub> value was 58.58 ppm while vitamin C had an IC<sub>50</sub> value of 4.50 ppm.</p>

scholarly journals Uji Aktivitas Antioksidan Ekstrak etanol Daun Kratom (Mitragyna Speciosa) Dengan Metode 1, 1 Difenil-2-Pikrihidrazil (DPPH)

2020 ◽  
pp. 204
Author(s):  
Herni Setyawati
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Mitragyna Speciosa ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Herbal Plants ◽  
Layer Chromatography ◽  
Positive Results

ABSTRACT Kratom (Mitragyna speciosa) is one of the herbal plants from Indonesia. Kratom plants have several benefits overcoming diarrhea, increase endurance, and deal with pain. Kratom leaf has several pharmacological effects, one of which is antioxidant. This study aims to determine the antioxidant activity of kratom leaf ethanol extract using the 1.1-Diphenyl-2-Pikrilhidrazil (DPPH) method expressed by IC50 values. Kratom leaves were extracted by the 3x24 hour remaceration method using 70% ethanol solvent, then concentrated with a rotary evaporator at 40 ° C, followed by an oven at a temperature (40-50) ° C to obtain a thick extract. Kratom leaf extract contains alkaloid compounds, flavonoids, tannins,and saponins. The antioxidant activity test was carried out with qualitative and quantitative tests.The qualitative antioxidant test was carried out using the Thin Layer Chromatography (TLC) method with n-hexane: ethyl acetate (7: 3) eluent, followed by DPPH spraying,which showed positive results giving antioxidant activity. The quantitative test for antioxidants was carried out using the 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) method using a  SpectrophotometerUV-Vis with vitamin C as a comparison. The quantitative antioxidant test was carried out using the 1,1-Diphenyl-2-Pikrilhidrazil (DPPH) method with vitamin C as a comparison. The results of antioxidant activity testing of kratom leaf ethanol extract showed an IC50 value of 91.86 ppm,so that it was included as a powerful antioxidant group. Keywords:Antioxidant,DPPH,Ethanol, IC50,Mitragyna speciosa.

Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.) Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.)

2019 ◽  
Vol 9 (2) ◽  
pp. 102
Author(s):  
Praise Meningkas ◽  
Dingse Pandiangan ◽  
Febby Kandou
Keyword(s):  
Vitamin C ◽  
Mtt Assay ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Test Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Cancer ◽  
Calculation Results ◽  
Anti Oxidant

Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.)Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.) Praise Frena Maningkas1*), Dingse Pandiangan1), Febby Ester Fany Kandou1)1)Jurusan Biologi, FMIPA, UNSRAT, Manado*E-mail: [email protected] Diterima 10 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Tujuan penelitian ini adalah untuk mendapatkan informasi ilmiah mengenai potensi antikanker dan antioksidan ektrak metanol serbuk daun pasote. Pengujian antikanker dilakukan dengan metoda uji MTT pada sel leukemia P388.Uji antioksidan dari ekstrak metanol, ditentukan dengan Spektro UV-Vis pada panjang gelombang 517 nm dengan metoda DPPH. Hasil pengamatan dan pengukuran diuji dengan program Origin Lab untuk antikanker, dan program Excel untuk analisis data antioksidan. Ekstak metanol pasote memiliki aktivitas yang kuat sebagai antikanker. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah adalah 53,37µg/mL termasuk kategori kuat sebagai antikanker. Nilai IC50 Quersetin adalah 87,78 μg/mL termasuk kategori kuat juga karena <100 μg/mL. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah adalah 50,131 µg/mL termasuk kategori sangat kuat sebagai antioksidan. Nilai IC50  vitamin C 48,533 μg/mL termasuk kategori sangat kuat juga karena <50 μg/mL. Hal ini menunjukkan bahwa ektrak air daun pasote potensial untuk dijadikan antioksidan dan tidak berbeda nyata dengan antioksidan vitamin C. Hal ini menunjukkan bahwa ektrak metanol daun pasote potensial untuk dijadikan antikanker dan antioksidan.Kata kunci: Antikanker, Antioksidan, Leukemia P388, MTT assay, DPPH ABSTRACT The aim of this study was to obtain scientific information about the anti-cancer and anti-oxidant potential of methanol extract of pasote. Anticancer testing was carried out by the MTT test method on P388 leukemia cells. The antioxidant test of methanol extract was determined by UV-Vis spectroscopy at a wavelength of 517 nm using the DPPH method. The results of observations and measurements were tested with the Origin Lab program for anticancer, and the Excel program for analysis of antioxidant data. The calculation results showed IC50 value of methanol extract was 53.37 µg / mL which was in the strong category as anticancer. The IC50 Quercetin value was 87.78 μg / mL which was in the strong category because it was <100 μg / mL. The results of the research obtained were pasote methanol extract have strong activity as antioxidants. The calculation results obtained IC50 value of methanol extract was 50.131 µg / mL including a strong category as an antioxidant. The IC50 value of vitamin C 48.533 μg / mL was also in a strong category because it was <50 μg / mL. This showed that pasote leaf extract is potential to be used as an anti-oxidant and not significantly different from vitamin C anti-oxidant. Overall, this study showed that epazote leaf extract is potential to be used as an anti-cancer and anti-oxidant.Keywords: Anti-cancer, Anti-oxidant, Leukemia P388, MTT assay, DPPH.

scholarly journals Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.) Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.)

2019 ◽  
Vol 9 (2) ◽  
pp. 102
Author(s):  
Praise Maningkas ◽  
Dingse Pandiangan ◽  
Febby Kandou
Keyword(s):  
Vitamin C ◽  
Mtt Assay ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Test Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Cancer ◽  
Calculation Results ◽  
Anti Oxidant

Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.)Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.) Praise Frena Maningkas1*), Dingse Pandiangan1), Febby Ester Fany Kandou1)1)Jurusan Biologi, FMIPA, UNSRAT, Manado*E-mail: [email protected] Diterima 10 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Tujuan penelitian ini adalah untuk mendapatkan informasi ilmiah mengenai potensi antikanker dan antioksidan ektrak metanol serbuk daun pasote. Pengujian antikanker dilakukan dengan metoda uji MTT pada sel leukemia P388.Uji antioksidan dari ekstrak metanol, ditentukan dengan Spektro UV-Vis pada panjang gelombang 517 nm dengan metoda DPPH. Hasil pengamatan dan pengukuran diuji dengan program Origin Lab untuk antikanker, dan program Excel untuk analisis data antioksidan. Ekstak metanol pasote memiliki aktivitas yang kuat sebagai antikanker. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah 53,37µg/mL termasuk kategori kuat sebagai antikanker. Nilai IC50 Quersetin adalah 87,78 μg/mL termasuk kategori kuat juga karena <100 μg/mL. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah adalah 50,131 µg/mL termasuk kategori sangat kuat sebagai antioksidan. Nilai IC50  vitamin C 48,533 μg/mL termasuk kategori sangat kuat juga karena <50 μg/mL. Hal ini menunjukkan bahwa ektrak air daun pasote potensial untuk dijadikan antioksidan dan tidak berbeda nyata dengan antioksidan vitamin C. Hal ini menunjukkan bahwa ektrak metanol daun pasote potensial untuk dijadikan antikanker dan antioksidan.Kata kunci: Antikanker, Antioksidan, Leukemia P388, MTT assay, DPPH ABSTRACT The aim of this study was to obtain scientific information about the anti-cancer and anti-oxidant potential of methanol extract of pasote. Anticancer testing was carried out by the MTT test method on P388 leukemia cells. The antioxidant test of methanol extract was determined by UV-Vis spectroscopy at a wavelength of 517 nm using the DPPH method. The results of observations and measurements were tested with the Origin Lab program for anticancer, and the Excel program for analysis of antioxidant data. The calculation results showed IC50 value of methanol extract was 53.37 µg / mL which was in the strong category as anticancer. The IC50 Quercetin value was 87.78 μg / mL which was in the strong category because it was <100 μg / mL. The results of the research obtained were pasote methanol extract have strong activity as antioxidants. The calculation results obtained IC50 value of methanol extract was 50.131 µg / mL including a strong category as an antioxidant. The IC50 value of vitamin C 48.533 μg / mL was also in a strong category because it was <50 μg / mL. This showed that pasote leaf extract is potential to be used as an anti-oxidant and not significantly different from vitamin C anti-oxidant. Overall, this study showed that epazote leaf extract is potential to be used as an anti-cancer and anti-oxidant.Keywords: Anti-cancer, Anti-oxidant, Leukemia P388, MTT assay, DPPH.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BULBUS BAWANG DAYAK (Eleutherine americana Merr) DENGAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 451
Author(s):  
Rizkah V. Mokoginta ◽  
Herny E. I. Simbala ◽  
Karlah L.R Mansauda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Onion Plant ◽  
Dpph Method ◽  
Ic50 Values ◽  
Experimental Laboratory ◽  
Ethanol Extracts ◽  
Eleutherine Americana

ABSTRACTDayak onion plant (Eleutherine americana Merr.) or commonly called forest onion is a typical plant of Central Kalimantan that has been used by local peoples for cure a various types of diseases. This study aims to determine the activity of antioxidant compounds from Dayak bulb ethanol extracts from Kotamobagu City. This research method is an experimental laboratory. Initially the Dayak bulb sample was extracted maceration with 96% ethanol solvent then evaporated until a thick extract was obtained. As a parameter, the DPPH method (1,1-diphenyl-2-picrylhydrazyl) was used to test the antioxidant activity of the samples at concentrations of 50, 60, 70, 80 and 90 mg / L and as a comparison standard used Vitamin C with concentrations of 2, 4, 6, 8 and 10 mg / L. The results obtained showed antioxidant activity in Dayak bulb ethanol extracts with IC50 values of 41.46 mg / L and on vitamin C IC50 values of 1.04 mg / L. Based on these results it can be concluded that the onion bulb ethanol extract has very strong antioxidant activity. Keywords: Dayak Onion bulbs, Activity, Antioxidant, DPPH  ABSTRAK Tanaman bawang Dayak (Eleutherine americana Merr.) atau biasa disebut bawang hutan merupakan tanaman khas Kalimantan Tengah yang sudah dipergunakan masyarakat lokal sebagai obat berbagai jenis penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bulbus bawang Dayak yang berasal dari Kota Kotamobagu. Metode penelitian ini adalah eksperimental laboratorium. Awalnya sampel bulbus bawang Dayak diekstraksi maserasi dengan pelarut etanol 96% kemudian dievaporasi hingga didapatkan ekstrak kental. Sebagai parameter, digunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl) untuk pengujian aktivitas antioksidan sampel pada konsentrasi 50, 60, 70, 80 dan 90 mg/L dan sebagai standar pembanding digunakan Vitamin C dengan konsentrasi 2, 4, 6, 8 dan 10 mg/L. Hasil penelitian yang didapat menunjukkan aktivitas antioksidan pada ekstrak etanol bulbus bawang Dayak dengan nilai IC50 sebesar 41,46 mg/L dan pada vitamin C nilai IC50 sebesar 1,04 mg/L. Berdasarkan hasil tersebut dapat disimpulkan bahwa ekstrak etanol bulbus bawang Dayak memiliki aktivitas antioksidan yang sangat kuat. Kata kunci: Bulbus Bawang Dayak, Aktivitas, Antioksidan, DPPH

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL SPONS Ianthella basta DARI DESA TUMBAK KECAMATAN PUSOMAEN KABUPATEN MINAHASA TENGGARA

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 713
Author(s):  
Nurhati Anton ◽  
Adithya Yudistira ◽  
Jainer Pasca Siampa
Keyword(s):  
Antioxidant Activity ◽  
Coral Reef ◽  
Vitamin C ◽  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Positive Control ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
Ethanol Extracts

ABSTRACT Sponges are a component of coral reef biota that has bioactive compounds with a greater percentage of activity compared to compounds produced by terrestrial plants. This study aims to determine the presence of antioxidant activity in the ethanol extracts of sponge Ianthella basta from the waters of Tumbak Village. Ianthella basta sponge was extracted using maceration method with 96% ethanol solvent. Antioxidant activity testing was carried out using the DPPH method with a concentration of 100 µg / mL, 75 μg / mL, 50 μg / mL, and 25 μg / mL and Vitamin C p.a as positive control. Each sample was made three repetitions of the test. Testing using a UV-Vis spectrophotometer. The results showed that the ethanol extract of the Ianthella basta sponge had antioxidant activity with a percentage of 48.73% at a concentration of 100 µg / mL. Keywords: Ianthella basta sponge, Antioxidants, Extraction, DPPH  ABSTRAK Spons merupakan salah satu komponen biota penyusun terumbu karang yang mempunyai senyawa bioaktif dengan persentase keaktifan lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan di dalam ekstrak etanol Spons Ianthella basta dari perairan Desa Tumbak. Spons Ianthella basta diekstraksi menggunakan metode maserasi dengan pelarut etanol 96%. Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH dengan konsentrasi 100 µg/mL, 75 µg/mL, 50 µg/mL, dan 25 µg/mL dan Vitamin C p.a sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji. Pengujian menggunakan alat spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa ektrak etanol Spons Ianthella basta memiliki aktivitas antioksidan dengan persentase sebesar 48,73% pada konsentrasi 100 µg/mL. Kata Kunci: Spons Ianthella basta, Antioksidan, Ekstraksi, DPPH

scholarly journals Limpasu Pericarpium : an Alternative Source of Antioxidant From Borneo with Sequential Maceration Method

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Irfan Zamzani ◽  
Nita Triadisti
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Oxidation Process ◽  
Antioxidant Activities ◽  
Alternative Source ◽  
Dpph Method ◽  
Indigenous Plant ◽  
Ic50 Values

Antioxidants are substances that can slow down the oxidation process of free radicals. Limpasu plant (Baccaurea lanceolata (Miq) Muell. Arg), an indigenous plant of Borneo, is a natural antioxidant source. The purpose of this study was to determine the antioxidant activity of the limpasu pericarpium extract. The extraction of the limpasu pericarpium was done by maceration method using solvents with increasing polarity ranging from n-hexane, ethyl acetate, and methanol. Antioxidant activities of the three extracts were measured by the DPPH and FRAP methods. The IC50 values of n-hexane, ethyl acetate, and methanol extracts, as well as quercetin using the DPPH method were 517,45 µg/mL, 530,64 µg/mL, 10,63 µg/mL and 6,83 µg/mL, respectively. Meanwhile, the IC50 values obtained from FRAP method were 198,96 µg/mL, 190,07 µg/mL, 661,36 µg/mL, and 7,09 µg/mL, respectively. The results revealed that the methanol extract is more potent than other extracts tested for antioxidant activity.

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