scholarly journals INDUCING GENETIC VARIABILITY OF BLACK PEPPER (Piper nigrum L.) by IRRADIATION

2020 ◽  
Vol 10 (4) ◽  
pp. 166
Author(s):  
NURLIANI BERMAWIE
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Rapd Analysis ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Genetic Changes ◽  
Band Size ◽  
Almost All ◽  
Dna Size

<p>Genetic variability of black pepper germplasm in Indonesia is low. To broaden genetic variability, newly growth shoot tips from in vitro culture of black pepper var. LDL were y irradiated with doses 0, 0.3 0.6, 0.9, 1.2 and 1.5 krad. The treatments were designed in a complete block with five replications. The irradiaed plantlets were grown on MS medium. Response of the variety is described by recording an increase in leaves, shoots and node, numbers, plantlet height, and morphological abnormality in the irst vegetative mutation generation (MVI) and the second vegetative mutation generation (MV2). Ater 6 weeks, the plantlets were sub cultured and the leaves of MV2 were used for RAPD analysis. Six random primers were used for the study, i.e. OPC-01 (TTCGAGC- CAG), OPC-02 (GTGAGGCGTC), OPC-04 (CCGCATCTAC), OPC-05 (GATGACCGCC), OPC-06 (GAACGGACTC) and Abi 117.17 (GCTC- GTCAAC). The results showed that the lowest averages value on the increase of leaves, shoots, nodes and plantlets height al MVI are resulted at dose 1.5 krad, whereas dose 0.3 krad increases averages value on shoots and plantlet height. The highest percentage of abnormal leaves is resulted at dose 1.2 krad. Ater subculture, the MV2 plantlets showed higher averages value for almost all parameters observed than the untreated plantlets. The number of score able bands varied from 2-5 bands with molecular weight 0.4-12 kb. Thirty three bands were detected from the six primers, with OPC-01, OPC-04 and OPC-06 showed polymorphisms with 8 (24%) polymorphic bands. In OPC-01 one band with DNA size 1 -1.5 kb was absence rom the treated plants at dose 0.9-1.5 krad, while with OPC- 04, one band size 1 5 kb present only at 1.2 krad and with OPC-06 one band size 12 kb absence from 0.6 and 0.9 krad, and 3-5 bands size 1.5, 1.8 and bands with size 3-12 kb disappeared at dose 1.2 and 1.5 krad. The appearance and disappearance of bands may be related to the genetic changes due to y irradiation, and further exploration may be needed to ind how much genetic variation induced by irradiation in ield and the relationships with the changes in plant characters.</p><p>Key words: Piper nigrum L., mutation, irradiation, RAPD, genetic variation</p><p> </p><p><strong>ABSTRAK </strong></p><p><strong>Peningkatan keragaman genetik tanaman lada (Piper nigrum L.) dengan iradiasi sinar gamma</strong></p><p>Keragaman genetik plasma nutfah lada sempit, untuk meningkatkan keragaman genetik, mata tunas yang tumbuh dari biakan lada varietas LDL diradiasi dengan sinar y dengan dosis 0, 0.3 0.6, 0.9, 1.2, dan 1.5 krad. Perlakuan menggunakan rancangan acak lengkap dengan lima ulangan. Tunas hasil radiasi ditanam pada media MS. Respon tanaman terhadap perlakuan iradiasi dilakukan dengan mengamati peningkatan jumlah daun, tunas, buku, tinggi tanaman dan morfologi pada planlet hasil perbanyakan vegetatif generasi pertama (MVI) dan kedua setelah iradiasi (MV2). Tunas hasil perbanyakan sub-kultur setelah iradiasi (MV2) dianaltsa keragaman genetiknya dengan RAPD (Randomly Ampliied Polymorphic DNA) menggunakan enam primer acak, yaitu OPC-01 (TTCGAGCCAG), OPC-02 (GTGAGGCGTC), OPC-04 (CCGCATCTAC), OPC-05 (GATGACCGCC), OPC-06 (GAACGGACTC) dan Abi 117.17 (GCTCGTCAAC). Hasil penelitian menunjukkan bahwa iradiasi menyebabkan perubahan yang nyata pada planlet generasi pertama setelah perbanyakan vegetatif (MVI) terutama pada jumlah buku dan tinggi tanaman. tetapi tidak berbeda nyata untuk penambahan jumlah daun dan<br /><br />166 <br /><br />tunas. Nilai rata-rata penambahan jumlah daun, tunas, buku dan tinggi planlet terendah ditunjukkan oleh perlakuan iradiasi pada dosis 1.5 krad, sedangkan pada iradiasi 0.3 krad meningkatkan nilai rata-rata jumlah tunas dan tinggi planlet. Persentase daun abnormal diperoleh pada perlakuan 1.2 krad. Setelah sub-kultur, planlet generasi kedua setelah perbanyakan vegetatif (MV2) yang tumbuh menunjukkan nilai rata-rata yang lebih tinggi dari normal pada semua parameter. Persentase daun variegata pada MVI diperoleh dari perlakuan 1.2 krad tetapi pada MV2 diperoleh dari perlakuan 0.6 krad. Jumlah pita DNA yang terampliikasi berkisar antara 2-5 dengan berat molekul 0.4-12 kb. Tiga puluh tiga pita tcrdetcksi, 8 (24 %) pita diantaranya polimorfik, yang berasal dari primer OPC-01, OPC-04 dan OPC-06. Pada OPC-01 satu pita dengan ukuran 1-1.5 kb hilang dari perlakuan 0.9-1.5 krad, sementara pada OPC-04, satu pita dengan ukuran 1.5 kb muncul hanya pada perlakuan 1.2 krad dan pada OPC-06 satu pita 12 kb hilang dari perlakuan 0-6 dan 0.9 krad, 3-5 pita dengan ukuran 1.5 kb, 1.8 kb dan antara 3-12 kb hilang dari perlakuan 1.2 dan 1.5 krad. Hilang dan munculnya pita kemungkinan berhubungan dengan perubahan genetik akibat radiasi sinar y dan penelitian lanjut perlu dilakukan untuk menge¬ tahui tingkat keragaman yang ditimbulkan akibat iradiasi di lapang dan hubungannya dengan perubahan sifat terutama sifat yang mengunlungkan<br /><br />Kara kunci: Piper nigrum L„ Lada, mutasi, radiasi, RAPD, variasi genetik</p>

scholarly journals In vitro toxin production by Fusarium solani f. sp. piperis

2003 ◽  
Vol 28 (3) ◽  
pp. 229-235 ◽  
Author(s):  
Maria de Lourdes R. Duarte ◽  
Simon A. Archer
Keyword(s):  
Fusarium Solani ◽  
Biological Response ◽  
Black Pepper ◽  
Biologically Active ◽  
Piper Nigrum ◽  
Piper Betle ◽  
Toxic Metabolites ◽  
Culture Filtrates ◽  
Detached Leaves

Fusarium solani f. sp. piperis (teleomorph: Nectria haematococca f. sp. piperis), causal agent of root rot and stem blight on black pepper (Piper nigrum), produces secondary metabolites with toxigenic properties, capable of inducing vein discoloration in detached leaves and wilting in transpiring microcuttings. Production of F. solani f. sp. piperis (Fsp) toxic metabolites reached a peak after 25 days of static incubation on potato sucrose broth at 25 ºC under illumination. Changes in the pH of the culture filtrate did not alter the effect of toxic metabolites. However, when the pH was changed before the medium had been autoclaved, a more intense biological response was observed, with an optimum at pH 6.0. Isolates that produced red pigments in liquid cultures were more efficient in producing biologically active culture filtrates than those which produced pink coloured or clear filtrates suggesting that these pigments could be related to toxigenic activity. Detached leaves of seven black pepper cultivars and Piper betle showed symptoms of vein discoloration after immersion in autoclaved and non-autoclaved Fsp culture filtrates indicating the thermostable nature of these toxic metabolites.

scholarly journals DNA Stability Contrasts with Chromosome Variability in Allium Fistulosum Calli

2014 ◽  
Vol 56 (1) ◽  
pp. 66-72 ◽  
Author(s):  
Patryk Mizia ◽  
Dagmara Kwolek ◽  
Tomasz Ilnicki
Keyword(s):  
Genetic Similarity ◽  
Chromosomal Instability ◽  
Selection Pressure ◽  
Dna Polymorphism ◽  
Rapd Analysis ◽  
Polymorphic Band ◽  
Genetic Changes ◽  
Band Frequency ◽  
Callus Lines

Abstract RAPD analysis was applied to assess the degree of DNA polymorphism in A. fistulosum calli of high chromosomal instability. Nineteen of 24 randomly selected RAPD primers revealed scorable polymorphism between calli and seeds (reference material). Polymorphic band frequency was 55/237 in seeds and 36/233 in calli; variability on the DNA level was thus lower in calli than in seeds (15.4% vs. 23.2% of band positions). UPGMA analysis of Jaccard's coefficients confirmed the genetic similarity of the analyzed cultures. The most distinctive DNA changes in calli involved coincident loss of original bands or the appearance of novel bands. Seven such changes (4 losses, 3 gains) were observed. Our results suggest that changes on the chromosomal level and on the DNA level occurred independently of each other and that different callus lines underwent similar genetic changes during culture, presumably due to strong selection pressure effected by standard in vitro conditions.

scholarly journals Comparative Analysis of Intracellular and in vitro Antioxidant Activities of Essential Oil From White and Black Pepper (Piper nigrum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Ying Wang ◽  
Liang Wang ◽  
Jin Tan ◽  
Rong Li ◽  
Zi-Tao Jiang ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Cell Model ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Anti Cancer ◽  
Total Antioxidant ◽  
Therapeutic Tools ◽  
Median Effective Concentration

Ethnopharmacological Relevance: Pepper essential oils have potential immunomodulatory, anti-tumor, and anti-cancer activities. Pepper exhibits the potential to prevent or attenuate carcinogenesis as therapeutic tools. However, the related mechanism remains unelucidated.Aim of the Study: The present study aims to provide reasonable information for the explanation of the dissimilarity of the essential oils from white (WPEO) and black pepper (BPEO).Materials and Methods: WPEO, BPEO, and their single active component, as well as synthetic antioxidants, were compared by the cell model methods and chemical methods, including intracellular antioxidant activity (CAA), total antioxidant activities (TAA), superoxide radical (SR), hydroxyl radical (HR), DPPH radical (DR) scavenging activities and inhibition ability of lipoprotein lipid peroxidation (ILLP).Results: The median effective concentration (EC50) values (mg/mL) of the WPEO and BPEO of SR, HR, DR, and ILLP were 0.437 and 0.327, 0.486 and 0.204, 7.332 and 6.348, 0.688, and 0.624 mg/mL, respectively. The CAA units of WPEO and BPEO were 50.644 and 54.806, respectively. CAA, DR, and TAA of BPEO were significantly higher than those of WPEO (p &lt; 0.05). The BPEO and WPEO can be differentiated as the former have higher correlations with 3-carene, α-pinene, β-pinene, and limonene while the latter has a higher caryophyllene correlation. The WPEO and BPEO show a good intracellular scavenging ability of reactive oxygen species in HeLa cells.Conclusion: Generally, pepper oil has stronger activities than single components, indicating that pepper is a broad-spectrum natural antioxidant.

EXTRACTION, ISOLATION, CHEMICAL IDENTIFICATION AND IN VITRO UROLITHIATIC ACTIVITY OF PIPERINE FROM PIPER NIGRUM

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (11) ◽  
pp. 38-41
Author(s):  
Jyoti ◽  
Yogesh Murti ◽  
Krishn K. Agrawal ◽  
Keyword(s):  
Calcium Oxalate ◽  
Black Pepper ◽  
Semipermeable Membrane ◽  
Piper Nigrum ◽  
Chemical Identification ◽  
Calcium Oxalate Crystals ◽  
Chemical Reagents ◽  
Oxalate Crystals ◽  
The Family

Piper nigrum is belongs to the family Piperaceae. The aim of the present study was to extract, isolate and investigate in vitro urolithiatic activity of alkaloid (piperine) from black pepper and identification by chemical reagents. In vitro urolithiatic evaluation of piperine was performed by percentage dissolution and turbidity methods. Calcium oxalate crystals were synthetically prepared and packed in a semipermeable membrane in both methods. Results were compared statistically and it showed significant P​

scholarly journals Prebiotic Potential of Culinary Spices Used to Support Digestion and Bioabsorption

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Christine T. Peterson ◽  
Dmitry A. Rodionov ◽  
Stanislav N. Iablokov ◽  
Meredith A. Pung ◽  
Deepak Chopra ◽  
...  
Keyword(s):  
Human Subjects ◽  
Curcuma Longa ◽  
Glycosyl Hydrolase ◽  
Health Benefits ◽  
Zingiber Officinale ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Sugar Composition ◽  
The Impact

Although the impact of medicinal and culinary herbs on health and disease has been studied to varying extents, scarcely little is known about the impact of these herbs on gut microbiota and how such effects might contribute to their health benefits. We applied in vitro anaerobic cultivation of human fecal microbiota followed by 16S rRNA sequencing to study the modulatory effects of 4 culinary spices: Curcuma longa (turmeric), Zingiber officinale (ginger), Piper longum (pipli or long pepper), and Piper nigrum (black pepper). All herbs analyzed possessed substantial power to modulate fecal bacterial communities to include potential prebiotic and beneficial repressive effects. We additionally analyzed the sugar composition of each herb by mass spectrometry and conducted genome reconstruction of 11 relevant sugar utilization pathways, glycosyl hydrolase gene representation, and both butyrate and propionate biosynthesis potential to facilitate our ability to functionally interpret microbiota profiles. Results indicated that sugar composition is not predictive of the taxa responding to each herb; however, glycosyl hydrolase gene representation is strongly modulated by each herb, suggesting that polysaccharide substrates present in herbs provide selective potential on gut communities. Additionally, we conclude that catabolism of herbs by gut communities primarily involves sugar fermentation at the expense of amino acid metabolism. Among the herbs analyzed, only turmeric induced changes in community composition that are predicted to increase butyrate-producing taxa. Our data suggests that substrates present in culinary spices may drive beneficial alterations in gut communities thereby altering their collective metabolism to contribute to the salubrious effects on digestive efficiency and health. These results support the potential value of further investigations in human subjects to delineate whether the metabolism of these herbs contributes to documented and yet to be discovered health benefits.

RAPD analysis off genetic variation in the Australian fan flower, Scaevola

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 600-606 ◽  
Author(s):  
Ines Swoboda ◽  
Prem L. Bhalla
Keyword(s):  
Genetic Variation ◽  
Genetic Variability ◽  
Genetic Distance ◽  
Rapd Analysis ◽  
Genetic Distances ◽  
Randomly Amplified Polymorphic Dna ◽  
Similarity Coefficients ◽  
Arbitrary Primers ◽  
Australian Species ◽  
Commercial Cultivars

The use of randomly amplified polymorphic DNA (RAPD) to study genetic variability in Scaevola (family Goodeniaceae), a native Australian species used in ornamental horticulture, is demonstrated. Plants of the genus Scaevola are commonly known as "fan flowers," due to the fan-like shape of the flowers. Nineteen accessions of Scaevola (12 cultivated and 7 wild) were studied using 20 random decamer arbitrary primers. Eight primers gave a distinct reproducible amplification profile of 90 scorable polymorphic fragments, enabling the differentiation of the Scaevola accessions. RAPD amplification of genomic DNA revealed a high genetic variability among the different species of Scaevola studied. Molecular markers were used to calculate the similarity coefficients, which were then used for determining genetic distances between each of the accessions. Based on genetic distances, a dendrogram was constructed. Though the dendrogram is in general agreement with the taxonomy, it also highlights discrepancies in the classification. The RAPD data showed that Scaevola aemula (series Pogogynae) is closer to Scaevola glandulifera of series Globuliferae than to the rest of members of series Pogogynae. In addition, the RAPD banding pattern of white flower S. aemula, one of the commercial cultivars, was identical to that of Scaevola albida, indicating their genetic similarity. Our study showed that there is a large genetic distance between commercial cultivars of Scaevola (Purple Fanfare, Pink Perfection, and Mauve Cluster), indicating considerable genetic variation among them. The use of RAPDs in intra- and inter-specific breeding of Scaevola is also explored.Key words: Scaevola, Australian native, RAPD, genetic distance, genetic variability.

scholarly journals INDUKSI DAN REGENERASI KALUS KELADI TIKUS (Typonium flagelliforme. Lodd. ) SECARA IN VITRO

2020 ◽  
Vol 13 (4) ◽  
pp. 142 ◽  
Author(s):  
SITTI FATIMAH SYAHID ◽  
NATALINI NOVA KRISTINA
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Carbon Source ◽  
Genetic Variability ◽  
In Vitro Culture ◽  
Basic Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Group B

<p>ABSTRAK<br />Keladi tikus umumnya diperbanyak secara vegetatif sehingga ragam<br />genetiknya sempit. Penelitian peningkatan keragaman genetik pada keladi<br />tikus melalui kultur in vitro telah dilakukan di Laboratorium Kultur<br />Jaringan, Balai Penelitian Tanaman Obat dan Aromatik (Balittro) Bogor<br />pada bulan April sampai Desember 2005. Bahan tanaman yang digunakan<br />adalah daun steril keladi tikus in vitro. Media dasar yang digunakan adalah<br />Murashige and Skoog (MS) yang diperkaya vitamin dari group B. Sebagai<br />sumber energi digunakan sukrosa sebanyak 30 g/l. Penelitian terdiri dari<br />dua tahap yaitu induksi dan regenerasi kalus. Perlakuan yang diuji pada<br />tahap I adalah beberapa taraf konsentrasi auksin (2,4-D) secara tunggal<br />maupun kombinasi dengan sitokinin (kinetin) terhadap induksi kalus yaitu<br />: 2,4-D 0,1 mg/l; 2,4-D 0,5 mg/l; 2,4-D 1,0 mg/l; 2,4-D 0,1 + kinetin 0,1<br />mg/l; 2,4-D 0,5 mg/l + kinetin 0,1 mg/l; 2,4-D 1.0 mg/l + kinetin 0,1 mg/l;<br />2,4-D 0,1 mg/l + kinetin 0,3 mg/l; 2,4-D 0,5 mg/l +kinetin 0,3 mg/l dan<br />2,4-D 1,0 mg/l + kinetin 0,3 mg/l. Tahap II adalah beberapa taraf<br />konsentrasi benzyl adenin untuk regenerasi kalus. Penelitian disusun<br />menggunakan rancangan acak lengkap dengan pola faktorial dan lima<br />ulangan, dan setiap ulangan terdiri dari satu eksplan. Faktor pertama<br />adalah asal kalus dan faktor kedua adalah beberapa taraf konsentrasi BA<br />yaitu : BA 0,1 mg/l ; BA 0,3 mg/l dan BA 0,5 mg/l. Parameter yang<br />diamati adalah waktu inisiasi kalus, struktur dan warna kalus, jumlah<br />tunas serta penampilan kultur secara visual. Hasil penelitian menunjukkan<br />bahwa kalus asal eksplan daun dapat diinduksi pada perlakuan 2,4-D 1,0<br />mg/l + kinetin 0,1 mg/l dan 2,4-D 1,0 mg/l + kinetin 0,3 mg/l dengan<br />waktu inisiasi 8 sampai 10 minggu setelah perlakuan. Regenerasi kalus<br />terbaik diperoleh pada medium 2,4-D 1,0 mg/l + kinetin 0,3 mg/l<br />mengandung BA 0,3 mg/l dengan rata-rata tunas dan daun yang dihasilkan<br />sebanyak 13,2 tunas dan 4,4 daun.<br />Kata kunci : Keladi tikus, Typonium flagelliforme Lodd., induksi,<br />regenerasi kalus, in vitro</p><p><br />ABSTRACT<br />Induction and regeneration of Rodent tuber calli through<br />in vitro culture<br />Rodent tuber plant (Typonium flagelliforme Lodd) is commonly<br />propagated vegetatively, the repro its genetic variation is narrow. A<br />research to increase the genetic variability of the plant was conducted in<br />Tissue Culture Laboratory of the Indonesian Medicinal and Aromatic<br />Research Institute, Bogor from April to December 2005. The leaf of<br />Rodent tuber in vitro used as an explants. Murashige and Skoog (MS)<br />medium used as basic medium, supplemented with vitamin from B group,<br />sucrose 30 g/l was added into the medium as carbon source. The research<br />consist of two steps : 1) calli induction and 2) calli regeneration. The<br />treatment tested in first step : 2.4-D 0.1 mg/l; 2.4-D 0.5 mg/l; 2.4-D 1,0<br />mg/l; 2.4-D 0.1 + kinetin 0.1 mg/l; 2.4-D 0.5 mg/l + kinetin 0.1 mg/l; 2.4-<br />D 1.0 mg/l + kinetin 0,1 mg/l; 2.4-D 0.1 mg/l + kinetin 0.3 mg/l; 2.4-D 0.5<br />mg/l + kinetin 0.3 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l. In the<br />second steps, several concentration of BA were tested i.e: BA 0,1 mg/l ;<br />BA 0,3 mg/l and BA 0,5 mg/l. The experiment was arranged in<br />completely randomized design with factorial pattern. Each treatment<br />consist of five replications. The parameters observed were time of calli<br />initiation, texture, colour of calli and number of shoot and leaves in<br />regeneration. The result showed that calli can be induced on 2.4-D 1.0<br />mg/l + kinetin 0.1 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l, eight to ten<br />weeks after culture. The best medium for shoots regeneration contains 2.4-<br />D 1.0 mg/l + kinetin 0.3 mg/l with 0.3 mg/l BA, with mean result of 13.2<br />shoots and 4.4 leaves.<br />Key words : Rodent tuber, Typonium flagelliforme Lodd. bl , induction,<br />regeneration, calli, in vitro</p>

scholarly journals Characterization of Pseudomonas bacteria of Piper tuberculatum regarding the production of potentially bio-stimulating compounds for plant growth

2021 ◽  
Vol 51 (1) ◽  
pp. 10-19
Author(s):  
Danyllo Amaral de OLIVEIRA ◽  
Solange da Cunha FERREIRA ◽  
Daiany Larissa Ribeiro CARRERA ◽  
Cleyson Pantoja SERRÃO ◽  
Daihany Moraes CALLEGARI ◽  
...  
Keyword(s):  
Plant Growth ◽  
Black Pepper ◽  
Plant Diseases ◽  
Piper Nigrum ◽  
Soluble Phosphate ◽  
High Concentration ◽  
Molybdenum Blue ◽  
Iaa Production

ABSTRACT Despite advances in the identification and characterization of endophytic bacteria in various plant species worldwide, little is known about such microorganisms in plants from the Amazon region. Previous studies reported that Piper tuberculatum endophytic Pseudomonas (isolates Pt12 and Pt13, identified as Pseudomonas putida and Pseudomonas sp., respectively) were able to inhibit the in vitro growth of Fusarium solani f. sp. piperis, which causes root rot in black pepper (Piper nigrum), and that Pt13 promoted the growth of P. nigrum. Therefore, the aim here was to characterize these bacteria regarding their ability to produce plant growth-promoting substances [siderophores, indol acetic acid (IAA) and soluble phosphate]. Chrome azurol S assays were performed for the detection of siderophores. For qualitative and quantitative assays of IAA production and phosphate solubilization, Salkowski´s reagent and NBRIP medium with molybdenum blue reagent, respectively, were used. Results revealed that Pt12 and Pt13 were able to synthesize IAA, mainly under a high concentration of L-tryptophan, indicating that they are IAA-producing bacteria, probably through a tryptophan-dependent biosynthesis pathway. The presence of P. nigrum extract positively influenced the IAA production by Pt12 and Pt13, with highest values of 125 and 90 µg mL-1, respectively. In addition, Pt12 was positive for the production of siderophores and produced 56.56 µg mL-1 of soluble phosphate. In contrast, Pt13 showed no ability to produce siderophores or to solubilize phosphate. Besides their potential in controlling plant diseases, Pt12 and Pt13 have potential as biofertilizers, favoring sustainable agriculture.

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