scholarly journals The effect of hot water and ethanol extract of Nigella sativa in immune system of Albino Mice

2009 ◽  
Vol 6 (2) ◽  
pp. 235-243
Author(s):  
Baghdad Science Journal
Keyword(s):  
Nigella Sativa ◽  
Water Extract ◽  
Ethanol Extract ◽  
Hot Water ◽  
Delayed Type Hypersensitivity ◽  
Differential Count ◽  
Alcoholic Extract ◽  
Albino Mice ◽  
Ethanol Extracts ◽  
High Level

The result showed that hot water and ethanol extracts of Nigella sativa contain alkaloids ,saponins, flavonoids,tannins,glycosides,terpins and steroids. Albino mice were administered orally with 0.1 of the extract at dose of 100 mg/kg, body weight the results showed high level of white blood cell ,total and differential count of WBC,phagocytosis index, mitotic index, Arthus and delayed type hypersensitivity. The result, also showed high level of hemoglobin (Hb) and the packed cell volume (PCV) the alcoholic extract has been found more efficient than hot water extract on mice.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Air dan Ekstrak Etanol Daun Kelor (Moringa Oleifera LAM)

2017 ◽  
Vol 6 (2) ◽  
pp. 125
Author(s):  
Rizkayanti Rizkayanti ◽  
Anang Wahid M. Diah ◽  
Minarni Rama Jura
Keyword(s):  
Vitamin C ◽  
Moringa Oleifera ◽  
Water Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Ic50 Value ◽  
Antioxidant Potency ◽  
Ethanol Extracts ◽  
Moringa Oleifera Lam

Moringa (moringa oleifera Lam) leaves contains many molecules as inhibitors for free radicals such as phenolic compounds (phenolic acids, flavonoids, quinones, coumarins, lignans, stilbenes, tannins), nitrogen compounds (alkaloids, amines, betalain), vitamins, terpenoids (including carotenoids), and several other endogenous metabolites as antioxidants. This study aimed to determine the antioxidant potency of water and ethanol extracts of moringa (moringa oleifera Lam) leave obtained by maceration and dekok. The concentration of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) was analyzed using UV-Vis spectrophotometer after addition of various concentrations of Moringa leaves extracts. Various concentrations of moringa leave extracts used were 20 ppm, 40 ppm, 60 ppm and 80 ppm. Vitamin C solutions as the positive control were prepared on similar various concentrations. The negative control was prepared using DPPH solutions dissolved in absolute ethanol. The results indicated that the ethanol extract of moringa leaves prepared by maceration method showed the antioxidant potency with an IC50 value of 22.1818 ppm, but the IC50 value of water extract of moringa leaves prepared by dekok was 57.5439 ppm. While, the IC50 value of Vitamin C was 8.8084 ppm. Based on the IC50 data it can be concluded that Vitamin C is a stronger antioxidant than moringa leaves extracts.

scholarly journals Chromatographic Analysis (LC-MS and GC-MS), Antioxidant Activity, Total Phenol and Total Flavonoid Determination of Ononis natrix L. Grown in Jordan

2021 ◽  
Vol 16 (1) ◽  
pp. 31-39
Keyword(s):  
Solid Phase ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Aroma Profile ◽  
Sesquiterpene Hydrocarbons ◽  
Leaf Oil ◽  
Terpenoid Aldehydes ◽  
Ethanol Extracts

The purpose of the present study was to identify, by gas chromatography (GC) and GC-mass spectrometry (GC-MS), the components of the spontaneously emitted volatile organic compounds (VOCs), obtained by Solid-Phase-Micro-Extraction (SPME) and that of the hydrodistilled oil of the fresh flowers and leaves of Ononis natrix, as well as to compare them. The hydrodistilled leaf oil was rich in non-terpenoid aldehydes, whereas its aroma profile contained mainly sesquiterpene hydrocarbons with α-copaene and germacrene D as their major components. The hydrodistilled oil of the fresh flowers, however, revealed nearly equal amounts of terpenoid and non-terpenoid compounds; 51.00% and 46.54%, respectively. The aroma profile of the fresh flowers was dominated by monoterpene hydrocarbons with α-pinene (42.96%) and α-thujene (20.17%) as the predomi¬nant two monoterpenes. Based on the high total phenol and flavonoid contents of the water and ethanol extracts, LC-MS analysis was carried out to identify the major compounds from each sample. From the water extract, eleven compounds were identified, whereas the ethanol extract contained eight, whereby luteolin (from the water extract) and apigenin (from the ethanol one) were named as the major flavonoids, respectively.

Resource Utilization Residue of Pleurotus ostreatus

2013 ◽  
Vol 807-809 ◽  
pp. 2048-2053
Author(s):  
Yong Xiong ◽  
Chun Yan Zhao ◽  
Yi Jian Chen ◽  
Wan Song Yue
Keyword(s):  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Flammulina Velutipes ◽  
Edible Fungi ◽  
Mycelium Growth ◽  
Extract Concentration ◽  
Ethanol Extracts ◽  
Better Than

The allelopathy of spent substrate extracts including water extract and ethanol extract ofPleurotus ostreatuson the mycelium growth of six edible fungi, i.e.Flammulina velutipes,Ganoderma lucidum Karst,Pleurotus ostreatusandCordyceps (three species), were investigated using Petri dishes approach. The results indicated that the spent substrate extracts have different effects on their mycelium growth. The mycelium ofFlammulina velutipesgrows better than control check with increasing water extract concentration. The mycelia ofGanoderma lucidum Karstgrow first promotion after inhibition with increasing water extract concentration. The mycelia ofFlammulina velutipes, Ganoderma lucidum Karst, Pleurotus ostreatusare promoted by ethanol extracts. The mycelia of Cordyceps (three species) grow first promotion after inhibition with ethanol extracts. The results can provide reference values for rational utilization of the spent mushroom substrate.

scholarly journals Protective effect of medicinal fungus Xylaria nigripes mycelia extracts against hydrogen peroxide-induced apoptosis in PC12 cells

2017 ◽  
Vol 30 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Rupesh D Divate ◽  
Pei-Ming Wang ◽  
Chiun-Chuang Wang ◽  
Su-Tze Chou ◽  
Chen-Tien Chang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Pc12 Cells ◽  
Water Extract ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Hot Water ◽  
Neuroprotective Effects ◽  
Medicinal Fungus ◽  
Induced Apoptosis

Xylaria nigripes ( XN) is a medicinal fungus that was used traditionally as a diuretic, nerve tonic, and for treating insomnia and trauma. In this study, we elucidated possible mechanisms of neuroprotective effects of XN mycelia extracts. XN mycelia were produced by fermentation. Hot water extract and 70% ethanol extract of XN mycelia were evaluated on hydrogen peroxide (H2O2)-induced apoptosis in PC12, a rat pheochromocytoma cell line. Both XN extracts effectively protected PC12 cells against H2O2-induced cell damage by inhibiting release of lactate dehydrogenase, decreasing DNA damage, restoring mitochondrial membrane potential, and arresting abnormal apoptosis through upregulation of Bcl-2 and downregulation of Bax and caspase 3. Compared to water extract, ethanol extract showed not only greater neuroprotective effects but also a higher antioxidant activity by scavenging DPPH radicals, inhibiting lipid peroxidation, and reducing power. High phenolic content and antioxidant activity may provide the neuroprotective properties of XN ethanol extract.

scholarly journals Study on Cardiotoxicity and Mechanism of “Fuzi” Extracts Based on Metabonomics

2018 ◽  
Vol 19 (11) ◽  
pp. 3506 ◽  
Author(s):  
Guangyao Huang ◽  
Liang Yang ◽  
Wei Zhou ◽  
Xianglin Tang ◽  
Yuguang Wang ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Mammalian Target Of Rapamycin ◽  
Water Extract ◽  
Ethanol Extract ◽  
Transforming Growth Factor Β1 ◽  
Heart Tissue ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Ethanol Extracts ◽  
Tgf Β1

To investigate the toxicity of water and ethanol “Fuzi” (FZ) extracts and to explore the toxicity mechanism in rats. Water and ethanol extracts were prepared. Three groups of rats received the water extract, ethanol extract, or water by oral gavage for seven days. Pathological section staining of heart tissue. Colorimetric analysis was used to determine serum lactate dehydrogenase. The metabolic expression of small molecules in rats was measured by a metabolomics method. Western blotting was used to detect the expression of phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), transforming growth factor-β1 (TGF-β1), and caspase-3. Immunohistochemistry was used to detect the expression of CTnI, mTOR, and TGF-β1. The water and ethanol FZ extracts exert cardiotoxic effects via activating the PI3K/Akt/mTOR signaling pathway to induce cardiomyocyte apoptosis.

scholarly journals Aktivitas Antioksidan dan Total Polisakarida Ekstrak Rumput Laut Merah, Hijau dan Coklat dari Pantai Jangkar Situbondo

2019 ◽  
Vol 16 (2) ◽  
pp. 64
Author(s):  
Didin Erma Indahyani ◽  
Depi Praharani ◽  
Izzata Barid ◽  
Ari Tri Wanodyo Handayani
Keyword(s):  
Antioxidant Activity ◽  
Brown Seaweed ◽  
Water Extract ◽  
Ethanol Extract ◽  
Extraction Methods ◽  
Red Seaweed ◽  
Water Extracts ◽  
High Antioxidant Activity ◽  
Polysaccharide Composition ◽  
Ethanol Extracts

The antioxidant activity and polysaccharide composition of seaweed is influenced by species (red, green and brown seaweed), geographical conditions, extraction methods, solvents, temperature and the length of time of extraction. The purpose of this study was to known the total polysaccharide and antioxidant activity in red, green and brown seaweed extracts from Jangkar Situbondo beach. Method. The study was conducted on red, green and brown seaweed extracted with ethanol and water to determine total polysaccharides. Antioxidant analysis uses the method 2,2-diphenyl1-picrylhydrazyl (DPPH) radical. Total polysaccharide and antioxidant activity were observed statistically using two-way ANOVA. The results of this study show that the polysaccharide content in seaweed water extract was significantly higher than ethanol extract, especially in brown seaweed. Seaweed water extracts showed higher antioxidant activity than ethanol extracts. The antioxidant activity of brown seaweed was significantly higher than that of green and red seaweed. Conclusion. Seaweeds have high antioxidant activity in water extracts. Brown seaweed water extract contains high polysaccharides than green and red seaweed. Antioxidant activity in brown seaweed is also higher than in green and red seaweed.

scholarly journals The influence of Rhus coriaria seeds Extracts on the Genetic Resistance of Aspergillus amstelodami

2018 ◽  
Vol 6 (2) ◽  
pp. 1-9
Author(s):  
Al-Hyaly F M ◽  
Al-Rawi J M
Keyword(s):  
Nitrous Acid ◽  
Genetic Resistance ◽  
Resistance Mutation ◽  
Water Extract ◽  
Ethanol Extract ◽  
Induced Mutations ◽  
Rhus Coriaria ◽  
Ethanol Extracts ◽  
Seed Extracts ◽  
Resistance Mutants

Introduction: This study aims to investigate the safe use of Rhus coriaria seed extracts (water and ethanol) genetically on fungi Aspergillus amstelodami because the extensive use of plant in many pharmaceutical and food fields. Materials and methods: In this study A1 (Wa1) strain of the fungus A.spergillus amstelodami was used in all genetic testing, Rhus coriaria seeds were obtained from the local markets and two types of extracts were prepared (water and ethanol extracts of Rhus coriaria seeds), two types of resistance mutants were isolated both spontaneous and induced by using mutation agent (nitrous acid). Results and Discussion: In this study 18 spontaneous resistance mutation were isolated in frequency 4.26×10-5 and 96 induced mutations were induced by nitrous acid in Frequency39.76×10-5at (MIC) 16 mg / ml of the ethanol extract, and 22 spontaneous mutations were isolate in frequency 4.59×10-5 and 91 nitrous acid induced mutation with an average recurrence 37.36×10-5at (MIC) 25 mg / ml of water extract. Conclusion: We conclude that presence of resistant ability in A. amstelodami toward Rhus coriaria water and ethanol seeds extracts. Therefore, we suggest further thorough studies to detect the activity to plant extract in order to be use in agricultural pest control.

In Vitro Anti-Inflammatory Activity of Hibiscus Schizopetalus (Dyer) Hook. f.

2020 ◽  
Vol 12 (4) ◽  
pp. 484-488
Author(s):  
M. R. Anusha ◽  
Veluswamy Bhuvaneshwari ◽  
Manokaran Kalaiselvi ◽  
Akbar Ali Amrin ◽  
Ramasamy Amsaveni
Keyword(s):  
Cold Water ◽  
Water Extract ◽  
Ethanol Extract ◽  
Hot Water ◽  
Inflammatory Activity ◽  
Plant Sample ◽  
Therapeutic Effects ◽  
Traditional Use ◽  
Anti Inflammatory

Medicinal plants act as an important source of drug with potential therapeutic effects. The present study focuses on the in vitro anti-inflammatory activity of Hibiscus schizopetalus (Dyer) Hook. f. (leaves and flowers) extracts. The cold water and hot water extract was prepared for plant sample without drying and whereas cold water, hot water, methanol, ethanol extract was prepared for the plant sample after drying. The present study on extract of H. schizopetalus demonstrated in vitro anti-inflammatory properties as evidenced by inhibition of albumin denaturation, membrane stabilization test and proteinase inhibitory action. This justifies that the traditional use of this plant in treatment of pains and inflammation.

scholarly journals Comparisons of the Anti-Inflammatory Activity of Dendropanax morbifera LEV Leaf Extract Contents Based on the Collection Season and Concentration of Ethanol as an Extraction Solvent

2020 ◽  
Vol 10 (23) ◽  
pp. 8756
Author(s):  
Kyeong Jin Kim ◽  
Ji Sun Youn ◽  
Young-Jun Kim ◽  
Ji Yeon Kim
Keyword(s):  
Inflammatory Cytokines ◽  
Inflammatory Responses ◽  
Ethanol Extract ◽  
Mapk Signaling ◽  
Hot Water ◽  
Mitogen Activated Protein Kinase ◽  
Inflammatory Activity ◽  
Dendropanax Morbifera ◽  
Anti Inflammatory ◽  
Ethanol Extracts

This study aimed to compare the anti-inflammatory activity of 10-year-old Dendropanax morbifera LEV (DM) leaf extracts. The leaves were collected during different seasons (May, August, and November), and the extracts were prepared using different methods (hot water, 30% ethanol, or 60% ethanol). Lipopolysaccharide-stimulated RAW264.7 cells were treated with these extracts for 12 h. The anti-inflammatory effects were evaluated by measuring the production of nitrite; prostaglandin E2 (PGE2); and inflammatory cytokines such as interleukin (IL)-6, IL-1β, and tumor necrosis factor-alpha, in addition to the mRNA expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 and activation of the nuclear factor κB (NF-κB)/mitogen-activated protein kinase (MAPK) pathways. The amyrin and polyphenol compositions of the extracts were analyzed using a triple time-of-flight mass spectrometer and high-performance liquid chromatography. The 30% ethanol extract harvested in May and 60% ethanol extracts collected in August and November displayed the highest inhibitions of nitrite, PGE2, and inflammatory cytokines. The 60% ethanol extract harvested in August suppressed activation of the NF-κB and MAPK signaling pathways. The contents of amyrin and polyphenol compounds were highly dependent on the ethanol concentration used during each season. These results suggest that ethanol extracts of DM leaves may have the potential to regulate inflammatory responses.

scholarly journals Phytochemical Comparison of the Water and Ethanol Leaf Extracts of the Cree medicinal plant, Sarracenia purpurea L. (Sarraceniaceae)

2015 ◽  
Vol 18 (4) ◽  
pp. 484 ◽  
Author(s):  
Carolina Cieniak ◽  
Brendan Walshe-Roussel ◽  
Rui Liu ◽  
Asim Muhammad ◽  
Ammar Saleem ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Betulinic Acid ◽  
Water Extract ◽  
Ethanol Extract ◽  
Hot Water ◽  
Active Principle ◽  
Sarracenia Purpurea ◽  
Leaf Extracts ◽  
Hot Water Extract ◽  
Phenolic Substances

Purpose: The Cree of Eeyou Istchee in Northern Quebec identified Sarracenia purpurea L. as an important plant for the treatment of Type 2 diabetes. Traditionally the plant is used as a decoction (boiling water extract) of the leaf, however, in order to study the extract in a laboratory setting, an 80% ethanol extract was used. In this study, the phytochemistry of both extracts of the leaves was compared and quantified. Methods: Two S. purpurea leaf extracts were prepared, one a traditional hot water extract and the other an 80% ethanol extract. Using UPLC-ESI-MS, the extracts were phytochemically compared for 2 triterpenes, betulinic acid and ursolic acid, using one gradient method and for 10 additional substances, including the actives quercetin-3-O-galactoside and morroniside, using a different method. Results: The concentrations of the nine phenolic substances present, as well as an active principle, the iridoid glycoside morroniside, were very similar between the two extracts, with generally slightly higher concentrations of phenolics in the ethanol extract as expected. However, two triterpenes, betulinic acid and ursolic acid, were 107 and 93 times more concentrated, respectively, in the ethanol extract compared to the water extract. Conclusion: The main phytochemical markers and most importantly the antidiabetic active principles, quercetin-3-O-galactoside and morroniside, were present in similar amounts in the two extracts, which predicts similar bioactivity.This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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