scholarly journals Comparison between the efficiency of serological tests for Identification of Brucellosis

2010 ◽  
Vol 7 (2) ◽  
pp. 901-909
Author(s):  
Baghdad Science Journal
Keyword(s):  
Rose Bengal ◽  
Serological Test ◽  
Elisa Test ◽  
Health Centers ◽  
Confirmatory Test ◽  
Igg Antibodies ◽  
Serological Tests ◽  
Primary Screening ◽  
Low Concentrations ◽  
Immune Assay

Five serological methods for detection of Brucella were compaired in this study, Four of the methods are commonely used in the detections:- 1-Rose-Bengal: as primary screening test which depends on detecting antibodies in the blood serum. 2-IFAT: which detects IgG and IgM antibodies in the serum. 3-ELISA test: which detects IgG antibodies in the serum. 4-2ME test: which detects IgG antibodies The fifth methods. It was developed by a reasercher in one of the health centers in Baghdad. It was given the name of spot Immune Assay (SIA). Results declares that among (100) samples of patients blood, 76, 49, 49, 37, and 28. samples were positive to Rose Bengal, ELISA, SIA, 2ME and IFAT tests, respectively. When efficiency, sensitivity and specificity of the serological methods were compaired, the Following results were obtained: a) ELISA and SIA were superiors among the other confirming methods (2ME and IFAT) in detecting the highest cases (49 cases); 46 of them were from the (76) cases positive to Rose Bengal The confirmatory test 2ME was not efficient in detecting low concentrations of IgG antibodies when less than half (37) of the total positive cases (76) were detected by this test. b) IFAT test was the least efficient confirmatory test among all other test. c) As a new confirmatory test, SIA proved to be an efficient and serological test for Brucella detection in comparison with other tests. It is an easy to use test, rapid and could be performed without need to the expensive equipment .

scholarly journals Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area

2012 ◽  
Vol 45 (4) ◽  
pp. 510-513 ◽  
Author(s):  
Teiliane Rodrigues Carneiro ◽  
Marta Cristhiany Cunha Pinheiro ◽  
Sara Menezes de Oliveira ◽  
Ana Lúcia de Paula Hanemann ◽  
José Ajax Nogueira Queiroz ◽  
...  
Keyword(s):  
Serological Test ◽  
Laboratory Diagnosis ◽  
Elisa Test ◽  
Northeastern Brazil ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Tests ◽  
Predictive Values ◽  
Transmission Area ◽  
Elisa Technique ◽  
Endemic Areas

INTRODUCTION: The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low endemic areas. METHODS: To increase sensitivity, we conducted further slide readings from the same stool sample using the parasitological method associated with a serological test. We used the KK method (three slides) and the IgG anti-Schistosoma mansoni-enzyme-linked immunosorbent assay (ELISA) technique to diagnose schistosomiasis in low endemic areas in the Brazilian State of Ceará. Fecal samples and sera from 250 individuals were analyzed. RESULTS: Sixteen percent and 47.2% of samples were positive in parasitological tests and serological tests, respectively. Parasitological methods showed that 32 (80%) individuals tested positive on the first slide, 6 (15%) on the second slide, and 2 (5%) on the third. The performance of the ELISA test in the diagnosis, using the KK method as diagnostic reference, showed a negative predictive value of 100%, with specificity and positive predictive values of 62.8% and 33.9%, respectively. CONCLUSIONS: In this study, the increase from one to three slides analyzed per sample using the KK technique was shown to be a useful procedure for increasing the diagnostic sensitivity of this technique.

Brucella abortus Detection by Indirect Competitive ELISA and Rose-bengal tests in Stray Dogs of Some Rural Areas in Wasit,Al-Qadisiyah and Dhi-QarProvinces / Iraq

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Saba Abood Ali
Keyword(s):  
Rose Bengal ◽  
Brucella Abortus ◽  
Rural Areas ◽  
Elisa Test ◽  
Competitive Elisa ◽  
Serological Tests ◽  
Stray Dogs ◽  
Indirect Competitive Elisa ◽  
Specific Igg ◽  
Specific Igg Antibodies

Brucella abortus is a gram-negative bacterium afflicting,mainly cattle,but can be attacked the dogs causing brucellosis disease. In some rural areas of three Iraqi provinces (Wasit,Al-Qadisiyah and Dhi-Qar),the stray dogs submitted for two an effective serological tests (rose-bengal test and indirect competitive ELISA) to detect an existence of the specific IgG antibodies against B. abortus. The results of this study reported (28.89%) and (39.26%) as a total infection rate by rose-bengal and ELISA test,respectively. According to studied areas,the seropositive infection rates by rose-bengal and ELISA tests in (Wasit,Al-Qadisiyah and Dhi-Qar provinces) were (31.25% and 45.83%), (38.71% and 54.84%); and (21.43% and 25%),respectively. The significant differences were reported between and within every examined province at level (P˂0.05).

Validation of a chemiluminescent assay for specific SARS-CoV-2 antibody

2020 ◽  
Vol 58 (8) ◽  
pp. 1357-1364 ◽  
Author(s):  
Marie Tré-Hardy ◽  
Alain Wilmet ◽  
Ingrid Beukinga ◽  
Jean-Michel Dogné ◽  
Jonathan Douxfils ◽  
...  
Keyword(s):  
Clinical Performance ◽  
Elisa Test ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Elisa Method ◽  
Serological Tests ◽  
Economy Of Scale

AbstractObjectivesFaced with the COVID-19 pandemic and its impact on the availability and quality of both therapeutic and diagnostic methods, the Belgian authorities have decided to launch a procedure for additional evaluation of the performance of serological tests offered for sale on the national territory. This has been proposed with a double aim: (1) an in-depth verification of the analytical and clinical performances presented by the manufacturer and (2) an economy of scale in terms of centralized validation for all the laboratories using the tests subject to evaluation.MethodsA retrospective validation study was conducted including the serum of 125 patients in order to determine the analytical and clinical performances of the LIAISON®SARS-CoV-2 from DiaSorin® detecting anti-SARS-CoV-2 IgG and to compare its clinical performance with the enzyme-linked immunosorbent assay (ELISA) test from Euroimmun®, one of the first commercially available tests allowing the detection of anti-SARS-CoV-2 IgA and IgG.ResultsThe performances of the LIAISON®SARS-CoV-2 satisfied all the acceptance criteria and provided “real world” analytical and clinical performances very close to the ones reported by the manufacturer in its insert kit. Comparison between the LIAISON®SARS-CoV-2 and the ELISA method did not reveal any difference between the two techniques in terms of sensitivities and specificities regarding the determination of the IgG.ConclusionsThis study reports the validation of the LIAISON®SARS-CoV-2 allowing to detect IgG antibodies specifically directed against SARS-CoV-2. The analytical and clinical performances are excellent, and the automation of the test offers important rates, ideal for absorbing an extension of testing.

scholarly journals Evaluation of antigenic variations between two virulent toxoplasma strains

2006 ◽  
Vol 55 (10) ◽  
pp. 1333-1335 ◽  
Author(s):  
Songul Bayram Delibas ◽  
Hatice Ertabaklar ◽  
Sema Ertug
Keyword(s):  
Toxoplasma Gondii ◽  
Western Blotting ◽  
Elisa Test ◽  
Test Results ◽  
Igg Antibodies ◽  
Serological Tests ◽  
Qualitative And Quantitative ◽  
Test Elisa ◽  
Molecular Masses ◽  
Immunofluorescence Antibody

Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies of 72 patients were examined with the indirect immunofluorescence antibody (IFA) test, ELISA and Western blotting (WB) by using antigen from both strains. Antigenic variations between strains did not affect IFA and ELISA test results, but qualitative and quantitative differences between the WB patterns were observed. A number of bands with molecular masses varying between 17 and 105 kDa were detected in WB. Fourteen different bands were obtained with the assay performed with RH strain antigen. An additional four bands were observed with TRH strain antigen. Also, an 80 kDa band was observed to stain darker in the blot with TRH strain antigen, whereas with RH strain antigen 30 and 38 kDa bands were darker. The results showed that strain-specific polymorphism in tachyzoite antigens of different Toxoplasma strains is important in the evaluation of WB but not in conventional serological analyses such as ELISA and IFA.

scholarly journals Towards a New Strategy for Diagnosis of Congenital Trypanosoma cruzi Infection

2017 ◽  
Vol 55 (5) ◽  
pp. 1396-1407 ◽  
Author(s):  
Alba Abras ◽  
Carmen Muñoz ◽  
Cristina Ballart ◽  
Pere Berenguer ◽  
Teresa Llovet ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Latin American ◽  
Serological Test ◽  
Igg Antibodies ◽  
Childbearing Age ◽  
Serological Tests ◽  
Content Type ◽  
Congenital Transmission ◽  
Screening Programs ◽  
New Strategy

ABSTRACTThe immigration of Latin American women of childbearing age has spread the congenital transmission of Chagas disease to areas of nonendemicity, and the disease is now a worldwide problem. Some European health authorities have implemented screening programs to prevent vertical transmission, but the lack of a uniform protocol calls for the urgent establishment of a new strategy common to all laboratories. Our aims were to (i) analyze the trend of passive IgG antibodies in the newborn by means of five serological tests for the diagnosis and follow-up of congenitalTrypanosoma cruziinfection, (ii) assess the utility of these techniques for diagnosing a congenital transmission, and (iii) propose a strategy for a prompt, efficient, and cost-effective diagnosis ofT. cruziinfection. In noninfected newborns, a continuous decreasing trend of passive IgG antibodies was observed, but none of the serological assays seroreverted in any the infants before 12 months. From 12 months onwards, serological tests achieved negative results in all the samples analyzed, with the exception of the highly sensitive chemiluminescent microparticle immunoassay (CMIA). In contrast, in congenitally infected infants, the antibody decline was detected only after treatment initiation. In order to improve the diagnosis of congenitalT. cruziinfection, we propose a new strategy involving fewer tests that allows significant cost savings. The protocol could start 1 month after birth with a parasitological test and/or a PCR. If negative, a serological test would be carried out at 9 months, which if positive, would be followed by another at around 12 months for confirmation.

scholarly journals Comparison of a Clinic-Based ELISA Test Kit with the Immunofluorescence Antibody Test for AssayingLeishmania infantumAntibodies in Dogs

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Daniela Proverbio ◽  
Eva Spada ◽  
Luciana Baggiani ◽  
Giada Bagnagatti De Giorgi ◽  
Roberta Perego
Keyword(s):  
Roc Curve ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Elisa Test ◽  
Standard Test ◽  
Confirmatory Test ◽  
Canine Leishmaniosis ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Test Kit

This study compares a rapid Immunospecific Kalazar Canine Rapid Spot IF with the gold standard test (indirect fluorescent antibody test (IFAT)) for detection ofLeishmania infantumspecific IgG serum antibodies in naturally exposed dogs. Serum samples were obtained from 89 healthy dogs and dogs affected by canine leishmaniosis (CanL). IgG-IFAT titers ≥80 were considered positive. Anti-L. infantumIgG antibodies were found in 54 samples with titers ranging from 1 : 80 to 1 : 5120. The performance of the rapid Immunospecific Kalazar was evaluated using a ROC curve. The area under the ROC curve of 0.957 was significantly different from 0.5 (), and therefore it can be concluded that the rapid Immunospecific Kalazar has the ability to distinguish canine sera with and withoutL. infantumIgG. The best performance of the test was at a cutoff >0 (sensitivity 92.6%, specificity 97%). The test can be used for disease screening if the cutoff is >0 (highest sensitivity, 92.6%) and is recommended as confirmatory test for the presence ofL. infantumIgG antibodies if the cutoff is set >2 (highest specificity, 100%).

scholarly journals Is the Status of CXCL9-CXCL10-CXCL11/CXCR3 axes Altered in Brucellosis Patients?

2021 ◽  
Author(s):  
Farzaneh Hassanshahi Hassanshahi ◽  
Gholamhossein Hassanshahi ◽  
Mojgan Noroozi karimabad
Keyword(s):  
Sandwich Elisa ◽  
Serum Levels ◽  
Elisa Test ◽  
Health Centers ◽  
Local Health ◽  
Serological Tests ◽  
Positive Correlation ◽  
The Status ◽  
History Of ◽  
Ifn Γ

Abstract Aim and objectives: Brucellosis is common in most parts of the world between humans and domestic animals. The causative agent is bacteria from the Brucella family, which are intracellular pathogens. Despite, Brucella spp. Is able to infect humans, their ability to induce production of chemokines by lung cells is unknown. In this study, we showed an association between brucellosis and CXCL9, 10 and 11chemokinesMethods:We have totally recruited 71 brucella patients who have referred to the either local health centers or hospitals of kerman province. Diagnosis of acute brucellosis was made based on a brief history of the disease,clinical examinations results of serological tests,antibody titration in serums was performed by bacterial wright method, agglutination and 2ME with antigen. Brucellosis patients CXCL9, CXCL10 and CXCL11 serum levels were detected by sandwich ELISA using paired chemokine-specific mAbs, according to the manufacturer’s guidelines. Transcription levels of mRNA for the CXCR3 as the chemokine receptor for CXCL9, CXCL10 and CXCL11 was examined by QRT-PCR using primers. Results: We have found that there was a positive correlation between the level of chemokines and their receptor. Here ELISA test showed that B. abortus induces expression of some IFN-γ induced CXCL9, 10 and 11chemokines in brucellosis patients. We have also showed the increased expression of CXCR3 as their shared receptors in brucellosis patients with real time-PCR. Conclusion: According to these findings there was a positive correlation between the level of chemokines and their receptor. We have found that there was a positive correlation between the level of chemokines and their receptor.

scholarly journals Comparison of diagnostic performances of ten different immunoassays detecting anti-CCHFV IgM and IgG antibodies from acute to subsided phases of Crimean-Congo hemorrhagic fever

2021 ◽  
Vol 15 (3) ◽  
pp. e0009280
Author(s):  
Petra Emmerich ◽  
Ronald von Possel ◽  
Christina Deschermeier ◽  
Salih Ahmeti ◽  
Lindita Berisha ◽  
...  
Keyword(s):  
Serological Test ◽  
Hemorrhagic Fever ◽  
Elisa Test ◽  
High Rate ◽  
Diagnostic Tools ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Crimean Congo Hemorrhagic Fever ◽  
Test Systems ◽  
Igm Antibodies

Crimean-Congo Hemorrhagic Fever Virus (CCHFV) is a geographically widespread tick-borne arbovirus that has been recognized by the WHO as an emerging pathogen needing urgent attention to ensure preparedness for potential outbreaks. Therefore, availability of accurate diagnostic tools for identification of acute cases is necessary. A panel comprising 121 sequential serum samples collected during acute, convalescent and subsided phase of PCR-proven CCHFV infection from 16 Kosovar patients was used to assess sensitivity. Serum samples from 60 healthy Kosovar blood donors were used to assess specificity. All samples were tested with two IgM/IgG immunofluorescence assays (IFA) from BNITM, the CCHFV Mosaic 2 IgG and IgM indirect immunofluorescence tests (IIFT) from EUROIMMUN, two BlackBox ELISAs for the detection of CCHFV-specific IgM and IgG antibodies (BNITM), two Anti-CCHFV ELISAs IgM and IgG from EUROIMMUN using recombinant structural proteins of CCHFV antigens, and two ELISAs from Vector-Best (IgM: μ-capture ELISA, IgG: indirect ELISA using immobilized CCHFV antigen). Diagnostic performances were compared between methods using sensitivity, specificity, concordance and degree of agreement with particular focus on the phase of the infection. In early and convalescent phases of infection, the sensitivities for detecting specific IgG antibodies differed for the ELISA test. The BlackBox IgG ELISA yielded the highest, followed by the EUROIMMUN IgG ELISA and finally the VectorBest IgG ELISA with the lowest sensitivities. In the subsided phase, the VectorBest IgM ELISA detected a high rate of samples that were positive for anti-CCHFV IgM antibodies. Both test systems based on immunofluorescence showed an identical sensitivity for detection of anti-CCHFV IgM antibodies in acute and convalescent phases of infection. Available serological test systems detect anti-CCHFV IgM and IgG antibodies accurately, but their diagnostic performances vary with respect to the phase of the infection.

scholarly journals Prevalência sorológica de Brucella spp. em porcos ferais e bovinos em simpatria no Pantanal do Mato Grosso do Sul, Brasil

2018 ◽  
Vol 39 (6) ◽  
pp. 2437
Author(s):  
Namor Pinheiro Zimmermann ◽  
Igor Alexandre Hany Fuzeta Schabib Peres ◽  
Paulo Henrique Braz ◽  
Raquel Soares Juliano ◽  
Luis Antônio Mathias ◽  
...  
Keyword(s):  
Rose Bengal ◽  
Diagnostic Tests ◽  
Comparative Test ◽  
Confirmatory Test ◽  
Serological Tests ◽  
Mato Grosso ◽  
Blood Samples ◽  
Feral Pigs ◽  
Fluorescence Polarization Assay ◽  
Mato Grosso Do Sul

The objective of this study was to determine the prevalence of anti-Brucella antibodies in feral pigs and bovines simpatrics in the Pantanal subregions of Paiaguás and Nhecolândia. The study was conducted in the municipality of Corumbá, Mato Grosso do Sul State, Brazil. A total of 105 feral pigs and 256 cattle were sampled in 12 farms, in all animals blood samples were collected for the serological diagnosis with Rose Bengal Test (RBT) for screening, 2-Mercaptoethanol (2-ME) confirmatory test and comparative test with Fluorescence Polarization Assay (FPA). The prevalence of positive feral pigs were 1% (1/105) in the RBT and FPA and no positive AAT results were confirmed in the 2-ME test. The prevalence of positive cattle sampled was 11.32%, 4.3% and 7.42% in the RBT, 2-ME and FPA tests respectively. The degree of agreement obtained between the serological tests used in cattle was Kappa = 0.506 (p <0.001), 95% CI (0.282 - 0.729). The results of the serological tests demonstrated that brucellosis is widespread in bovine herds of the region studied, but the same type of exposure to the agent did not occur in feral pigs according to the diagnostic tests used.

scholarly journals Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae

2021 ◽  
Vol 8 ◽  
Author(s):  
Mandy Carolina Elschner ◽  
Falk Melzer ◽  
Harisankar Singha ◽  
Saqib Muhammad ◽  
Ian Gardner ◽  
...  
Keyword(s):  
International Trade ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Elisa Test ◽  
Confirmatory Test ◽  
Test Results ◽  
Trade Restrictions ◽  
Field Samples ◽  
Financial Consequences

Glanders, caused by Burkholderia (B.) mallei is a notifiable zoonotic disease in equidae. For international trade and movement of equids, certificates of negative serological test results for antibodies against B. mallei are required. To date, the complement fixation test (CFT) is the mandatory test to issue these health certificates. The CFT is difficult to standardize and, due to its poor specificity, often leads to false-positive reactions resulting in trade restrictions with considerable financial consequences. In the present study, the new ID Screen Glanders Double Antigen Multispecies ELISA (GLANDA- ELISA) (IDvet, Grabels, France) was evaluated using 400 negative and 370 glanders positive field samples of equidae. The GLANDA-ELISA was significantly more specific (99.8%) than the CFT (97.0%). Considering the comparable sensitivities of CFT (96.5%) and ELISA (98.1%), this new GLANDA-ELISA test appears a suitable confirmatory test and a realistic alternative for serological testing of horses for trade or movement.

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