scholarly journals Effect of some species of bacteria on viability of human hydatid cysts

2010 ◽  
Vol 7 (3) ◽  
pp. 1153-1158
Author(s):  
Baghdad Science Journal
Keyword(s):  
Bacterial Infections ◽  
Public Hospital ◽  
Culture Media ◽  
Cell Activity ◽  
Culture Technique ◽  
Hydatid Cysts ◽  
Streptococcus Pneumonia ◽  
Hydatid Fluid ◽  
Negative Culture ◽  
Biliary Ducts

A total of 50 fertile human hydatid cases {33(66%) females and (34%) males}, obtained from Al-Ramadi public Hospital during the period from December 2003 to July 2004 were examined to study any bacterial infections. The specimens were obtained from hydatid fluid and then cultured on appropriate culture media to distinguish some species of bacteria which resulted in obtaining: Staphylococcus aureus (18%), Pseudomonas aeruginosa(12%), Escherichia coli(6%) and Streptococcus pneumonia (4%). These bacteria were confirmed by isolation from interacyst fluid and blood culture technique. The possible routs of infection may be through blood, biliary ducts and bronchioles .The selectivity permeable of the cyst wall may be absent and that may allow some species of bacteria to enter inside the cyst. Further, the percent viability decreased among cyst which yielded S. aureus , P. aeruginosa and other bacteria isolated after culturing compared with those of negative culture .Besides, the two types of protoscoleces motilities (flame cell activity and constriction –relaxation movement )increased in cases of negative culture .This association holds true at three different temperature (25ºC,37ºC and 40ºC).

scholarly journals Acellular Wharton’s Jelly, Potentials in T-Cell Subtypes Differentiation, Activation and Proliferation

2020 ◽  
Vol 10 (4) ◽  
pp. 617-622
Author(s):  
Mehdi Talebi ◽  
Hojjatollah Nozad Charoudeh ◽  
Ali Akbar Movassaghpour Akbari ◽  
Behzad Baradaran ◽  
Tohid Kazemi
Keyword(s):  
T Cells ◽  
T Cell ◽  
Culture Media ◽  
Cell Activity ◽  
Cell Subset ◽  
Wharton’S Jelly ◽  
Cellular Immunotherapy ◽  
Lag Phase ◽  
Wharton's Jelly ◽  
Study Results

Purpose : Because of different potentials of T-cell subtypes in T-cell based cellular immunotherapy approaches such as CAR-T cell therapies; Regarding the high cost of the serum-free specific culture media, having distinct control on T-cell subset activation, expansion and differentiation seem crucial in T-cell expansion step of cell preparation methods. By the way, there was no clear data about the effect of acellular Wharton’s Jelly (AWJ) on T-cells expansion, activation or differentiation status. So, we have launched to study the effect of AWJ on T-cell’s immunobiological properties. Methods: CD3+ T-cells were isolated from healthy bone marrow allogeneic donors, sorted by FACS method and cultured on either routine phyto-hemagglutinin complemented and different concentrations of AWJ, lag phase and doubling time of the cells calculated from cell growth curve. After 3, 7 and 14-days T-cell subtypes cell markers and cell activity related genes expression rate have been evaluated by flow cytometry and real-time polymerase chain reaction (PCR) methods respectively. Results: AWJ in a 1:1 ratio compared with contemporary lymphocyte culture media showed significant activating and proliferative capacities. The introduced condition has not affected the frequency of CD4+ subpopulation of T-cells, but significantly increased even CD8+ cells and immune-activator genes in T-cells. The regulatory and memory subsets of T-cells in this study have not affected significantly. Conclusion: the study results revealed that AWJ can be utilized as a supportive substance to increase the memory properties of the T-cells, gives control to design a selective medium for expanding and differentiating memory T-cells, relatively.

scholarly journals In-vitro evaluation of Nigella sativa and Punica granatum effect on protoscolices of hydatid cysts

2019 ◽  
Vol 28 (2) ◽  
pp. 210-214 ◽  
Author(s):  
Nasr Moawad El-Bahy ◽  
Amer Ragheb Abdelaziz ◽  
Reda Elbastawisy Khalafalla
Keyword(s):  
Anaphylactic Shock ◽  
Treatment Approach ◽  
Nigella Sativa ◽  
Punica Granatum ◽  
Hydatid Cysts ◽  
Hydroalcoholic Extract ◽  
Hydatid Fluid ◽  
Viability Assay ◽  
Peel Extract

Abstract Cystic echinococcosis (CE) are commonly found in the liver and lungs of affected hosts. The treatment approach is usually surgical, or giving drugs in conjunction before surgery to kill protoscolices, to avoid anaphylactic shock from leakage of hydatid fluid into the peritoneum and to decrease opportunities for recurrences. The present study was to evaluate the in vitro scolicidal efficacy of hydroalcoholic extract of Punica granatum peel and Nigella sativa, on the protoscolices of CE that collected from the lungs of infected camels. Different concentrations of extracts with different exposure times were used and a viability assay was applied to measure the scolicidal effect. N. sativa showed its highest scolicidal efficacy at 100 mg/mL and 10 mg/mL concentrations after 30 and 60 min. P. granatum peel extract showed its maximum scolicidal efficacy at 100 mg/mL concentration after 120 min. All experiments of the current study revealed that the extracts of both N. sativa and P. granatum had a scolicidal effects on the protoscolices of camel hydatid cysts. It could be concluded that N. sativa extract is more potent than P. granatum peel extract regarding scolicidal effect, but the efficacies of both extracts were of moderate significant correlation to exposure time and concentrations.

scholarly journals Biofilm Formation by Filamentous Fungi Recovered from a Water System

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Virgínia M. Siqueira ◽  
Nelson Lima
Keyword(s):  
Filamentous Fungi ◽  
Biofilm Formation ◽  
Distribution Systems ◽  
Water Distribution ◽  
Culture Media ◽  
Cell Activity ◽  
Water System ◽  
Drinking Water Distribution Systems ◽  
Drinking Water Distribution ◽  
Planktonic Form

Filamentous fungi have been constantly recovered from diverse aquatic environments including drinking water distribution systems. Although most of the works are focused on the study of planktonic form, recent researches have shown that fungi develop biofilm within these systems. In this study, Aspergillus sp. (section Nigri), Aspergillus sp. (section Flavi), Alternaria sp., Botrytis sp., Cladosporium sp., and Penicillium sp. recovered from water biofilms were used to evaluate their capability to grow as biofilms under laboratorial conditions. Morphological and physiological characteristics were analysed using image analysis and biomass and cell activity estimation. All six isolates were able to form biofilm, though different patterns of development were observed. Only Alternaria sp. formed biofilm in water over 24 h of analysis. MEB was shown to be the best culture media for biofilm formation. A direct correlation between biomass and cell activity was not observed, but biomass values and morphological parameters, that is, monolayer and EPS production, were directly correlated. Thus, the results present here highlight the capability of fungi to form biofilms and the emergent necessity to standardize methods for further research in this area.

A Fatal Case of Neonatal Adenovirus Infection

2010 ◽  
Vol 29 (5) ◽  
pp. 297-305 ◽  
Author(s):  
Cynthia Kelley
Keyword(s):  
Bacterial Infections ◽  
Early Recognition ◽  
Severe Disease ◽  
Fatal Case ◽  
Rapid Identification ◽  
Culture Technique ◽  
Adenovirus Infection ◽  
Shell Vial ◽  
Adenoviral Infection ◽  
Culture Techniques

Adenovirus can produce severe disease and even death in the immunocompromised neonate. Symptoms of adenovirus infection are similar to those seen with bacterial infections in neonates, making early recognition and diagnosis difficult. Consideration of adenovirus as a causative agent is important to early diagnosis. Currently available culture techniques, particularly the shell vial culture technique, make more rapid identification of adenovirus infection possible. Early identification and treatment are necessary to improve patient outcomes and prevent the spread of infection to other neonates. Available agents for the treatment of adenovirus have had mixed results, yet their use is preferable to nontreatment of critical patients. This article presents the case of a preterm infant who became fatally ill from disseminated adenoviral infection.

scholarly journals Screening of Actinomycetes from Turmeric (Curcuma longa L.) and Ginger (Zingiber officinale) Rhizosphere for Antifungal Activity

2020 ◽  
pp. 18-28
Author(s):  
R. C. Osaro-Matthew ◽  
F. S. Ire ◽  
N. Frank-Peterside
Keyword(s):  
Antifungal Activity ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Culture Media ◽  
Curcuma Longa ◽  
Zingiber Officinale ◽  
Culture Technique ◽  
Rrna Gene ◽  
Dual Culture ◽  
Test Organisms

Aim: The present study was carried out to isolate actinomycetes from rhizospheric soil of Curcuma longa and Zingiber officinale and evaluate their antifungal potential. Methods: Actinomycetes were isolated from the rhizosphere soil of two Zingiberaceae plants (Curcuma longa and Zingiber officinale), using four different culture media. Isolates were screened for antifungal activity using dual culture technique against two reference strains Colletotrichum coccodes (DSM 2492) and Alternaria pimpriana (DSM 62023). The most potent strain was identified based on 16S rRNA gene sequence and the bioactive components of the strain were identified using GC-MS chromatography. Results: Fifteen strains of actinomycetes were isolated, SCA (starch casein agar) was found best for cultivation of actinomycetes. The 15 strains were grouped into three genera Norcadia 8(54%), Streptomyces 5(33%) and Janibacter 2(13%) based on morphological, biochemical and physiological identification. Among the 15 isolates 6(40%) strains showed activity against either of the test organisms, while 1(6.7%) was active against the both test organisms. Comparative analysis of the 16S rRNA gene sequences identified the potent isolates as Janibacter sp. strain RC18. GC-MS analysis revealed the presence of 20 compounds with 10 identified as potent antimicrobial metabolites. Conclusion: This study has revealed that rhizosphere of ginger and turmeric harbours rare actinomycetes that are valuable tool for sustainable agriculture.

scholarly journals Prospective study of bedside inoculation of blood culture bottles with ascitic fluid versus delayed inoculation for the detection of spontaneous bacterial peritonitis

2017 ◽  
Vol 6 (1) ◽  
pp. 139
Author(s):  
Girish Iyyanna ◽  
Manjunath F. V. ◽  
Taruni Ng
Keyword(s):  
Blood Culture ◽  
Ascitic Fluid ◽  
Bacterial Infections ◽  
Spontaneous Bacterial Peritonitis ◽  
Culture Media ◽  
Positive Culture ◽  
Culture Methods ◽  
Cross Sectional ◽  
Bacterial Peritonitis ◽  
Bacterial Yield

Background: Spontaneous bacterial peritonitis (SBP) is the most common bacterial infection in cirrhosis, accounting for 10%-30% of all reported bacterial infections in the patients admitted to hospital. Spontaneous bacterial peritonitis (SBP) is the most frequent and life-threatening infection in patients with liver cirrhosis. All forms of cirrhosis have been reported to be complicated by SBP. A delay in the time period between the collection of the ascitic fluid sample, and its inoculation into the blood culture media, has been one of the reasons implicated to account for low-test positivity. There was lack of studies for comparing the bacterial yield between bedside inoculated blood culture bottles with ascitic fluid over delayed inoculation in the detection of SBP. Hence this study is done to compare the bacterial yield between bedside inoculated blood culture bottles with ascitic fluid over delayed inoculation for the detection of SBP.Methods: Cross sectional study.Results: Maximum number of cases of cirrhosis with ascites with SBP was seen in the age group of 31-40years (54.4%) with mean age of study population being 39.66years, more common in males, bed side inoculation yielded more positive culture reports compared to delayed inoculation and E. coli and klebsilla being the common organisms.Conclusions: Difference between 2 culture methods in isolating organism in SBP cases was not statically significant. But, among culture positive cases, this study demonstrates that bedside inoculation of blood culture bottles is superior to delayed laboratory inoculation.

scholarly journals The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Forests ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1211
Author(s):  
Rambod Abiri ◽  
Narges Atabaki ◽  
Hazandy Abdul-Hamid ◽  
Ruzana Sanusi ◽  
Nor Aini Ab Shukor ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Review Paper ◽  
Genetic Interaction ◽  
Culture Media ◽  
Forest Tree ◽  
Culture Technique ◽  
Reliable Technique ◽  
Current Review ◽  
Industrial Level

Micropropagation is a reliable technique in biotechnology and genetic engineering domain, which has been widely applied for rapid mass propagation of plants in vitro condition. Through micropropagation techniques, reproduction of plants can be attained from different explants using organogenesis and somatic embryogenesis. Over the decades, micropropagation techniques have offered tremendous potential for forest tree improvement. Eucalyptus is a woody plant species recalcitrant to in vitro culture. In general, the micropropagation of Eucalyptus culture processes and the genotype, environment surroundings, and age of explants in culture media is frequently linked with the occurrence of micropropagation variation. In the current review paper, an update of the most important physiological and molecular phenomena aspects of Eucalyptus micropropagation was linked to the most profound information. To achieve the mentioned target, the effect of plant growth regulators (PGRs), nutrients, other adjuvant and environmental features, as well as genetic interaction with morpho- and physiological mechanisms was studied from the induction to plant acclimatisation. On the other hand, important mechanisms behind the organogenesis and somatic embryogenesis of Eucalyptus are discussed. The information of current review paper will help researchers in choosing the optimum condition based on the scenario behind the tissue culture technique of Eucalyptus. However, more studies are required to identify and overcome some of the crucial bottlenecks in this economically important forest species to establish efficient micropropagation protocol at the industrial level.

Culture Media Composition and Reduction Potential Optimization of Mycelia-free Filtrate for the Biosynthesis of Silver Nanoparticles Using the Fungus Tritirachium oryzae W5H

2020 ◽  
Vol 16 (5) ◽  
pp. 757-769
Author(s):  
Muhamad Al-limoun ◽  
Haitham N. Qaralleh ◽  
Khaled M. Khleifat ◽  
Mohammed Al-Anber ◽  
Amjad Al-Tarawneh ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Culture Medium ◽  
Culture Media ◽  
Synthesis Process ◽  
Media Composition ◽  
Major Focus ◽  
Multiple Sources ◽  
Formation Reaction ◽  
Carbon And Nitrogen ◽  
Tritirachium Oryzae

Background: A major focus of nanotechnology concerns is the expansion of the optimization of nanomaterials in purity, size and dispersity. Methods: In the current work, a two-step AgNP synthesis process was optimized at the mycelia-DI water suspension and AgNP formation reaction levels. Results: Biomass filtrate from the fungal strain Tritirachium oryzae W5H was able to reduce silver nitrate into AgNPs after a 72 h reaction, as indicated by the development of intense brown color and by UV-vis spectra. The biosynthesis ability of AgNPs was markedly better in the presence of a single carbon and nitrogen source in the culture medium compared to multiple sources of carbon and nitrogen. The optimization results of AgNP formation were indifferent between the two steps and were 20 g biomass, 40°C, pH 7.0, 96 h and 1.0 mM AgNO3. The TEM images of the prepared AgNPs illustrated the presence of 7-75 nm, monodispersed and spherical- to ovular-shaped Ag nanoparticles. Conclusion: The present work highlights the importance of investigating the process parameters by which the reductant mycelia-free filtrate was prepared. In addition, we explored the promising antibacterial action of the prepared AgNPs against bacterial infections.

Prevalence and Types of Bacteria Associated With Ocular Infections of Patients Visiting the Optometry Clinic of Federal University of Technology Owerri

2021 ◽  
Author(s):  
Anastasia NneNna Ogu ◽  
Josephat N Okereke ◽  
Sylvia O Anyadoh-Nwadike ◽  
Chukwunonyerem Chika Ogwunga
Keyword(s):  
Bacterial Infections ◽  
Culture Media ◽  
Bacterial Species ◽  
Female Gender ◽  
Broth Culture ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Ocular Infections ◽  
University Of Technology ◽  
Peptone Broth

Abstract The prevalence and types of bacteria associated with ocular infections were studied using swab samples from ocular infected patients attending the Department of Optometry, Federal University of Technology, Owerri clinic. A total of fifty specimens were collected from patients comprising fourteen males and thirty-six females with ocular infections and analyzed aseptically in the Biotechnology laboratory within thirty minutes of collection. The samples were maintained on peptone broth in test tubes and about 1 ml of the overnight peptone broth culture was transferred into sterile petri dishes containing the culture media (nutrient, blood and macConkey agar). Standard microbiological and biochemical protocols were used for isolation, characterization and identification of the bacterial isolates. All specimens had bacterial growth. Fifty-seven bacterial isolates; 35 Gram positive and 22 Gram negative bacteria were identified. These fell into twelve species; Bacillus sp., Corynebacterium sp., Pseudomonas aeruginosa, Haemophilus sp., Staphylococcus aureus, Lactobacillus sp., Klebsiella sp., Citrobacter sp., Proteus mirabilis, Streptococcus pneumoniae, Listeria sp. and Neisseria sp. The predominant bacterial species isolated was Bacillus sp. 17 (29.8%) while Streptococcus sp., Listeria sp., and Neisseria sp. were the least with 1 (1.8%) each. The prevalence rate of bacteria was higher among the female gender within the age group 21 -30 years. The burden of bacterial infections of the eyes is high. The prevalence and types of bacteria may not be exactly the same in every part of the world. To mitigate the burden of ocular infections, physicians need to comply with etiologic approach of diagnosis and treatment regimen.

Effect of Temperature on the Viability of Protoscolices of Echinococcus granulosus invitro

2008 ◽  
Vol 32 (1) ◽  
pp. 13-21
Author(s):  
Amall Hassan Atia
Keyword(s):  
Echinococcus Granulosus ◽  
Effect Of Temperature ◽  
Hydatid Cysts ◽  
Survival Times ◽  
Vital Stain ◽  
Hydatid Fluid ◽  
Survival Percentage ◽  
Percentage Survival ◽  
Refrigerator Temperature

A study was conducted invitro to determine the effects of storage attemperature 4 Cº upon the survival of protoscolices of Echinococcusgranulosus from 86 hydatid cysts isolated from infected donkeys . Flam cellactivity was the choice for viability , in addition using vital stain (0.1%aqueous eosin) . The result showed that higher percentage survival 94.41%in hydatid fluid for 9 days . The longest survival times of any of the samplestested was 81 days with the survival percentage 85% and the lowest was 33%for 13 days , showing movement till the end of this period . Our resultsproved the protoscolices removed from cyst retain their survival for severalweeks at refrigerator temperature when stored in hydatid fluid in test tubes .

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