Prevalence and Types of Bacteria Associated With Ocular Infections of Patients Visiting the Optometry Clinic of Federal University of Technology Owerri

2021 ◽  
Author(s):  
Anastasia NneNna Ogu ◽  
Josephat N Okereke ◽  
Sylvia O Anyadoh-Nwadike ◽  
Chukwunonyerem Chika Ogwunga
Keyword(s):  
Bacterial Infections ◽  
Culture Media ◽  
Bacterial Species ◽  
Female Gender ◽  
Broth Culture ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Ocular Infections ◽  
University Of Technology ◽  
Peptone Broth

Abstract The prevalence and types of bacteria associated with ocular infections were studied using swab samples from ocular infected patients attending the Department of Optometry, Federal University of Technology, Owerri clinic. A total of fifty specimens were collected from patients comprising fourteen males and thirty-six females with ocular infections and analyzed aseptically in the Biotechnology laboratory within thirty minutes of collection. The samples were maintained on peptone broth in test tubes and about 1 ml of the overnight peptone broth culture was transferred into sterile petri dishes containing the culture media (nutrient, blood and macConkey agar). Standard microbiological and biochemical protocols were used for isolation, characterization and identification of the bacterial isolates. All specimens had bacterial growth. Fifty-seven bacterial isolates; 35 Gram positive and 22 Gram negative bacteria were identified. These fell into twelve species; Bacillus sp., Corynebacterium sp., Pseudomonas aeruginosa, Haemophilus sp., Staphylococcus aureus, Lactobacillus sp., Klebsiella sp., Citrobacter sp., Proteus mirabilis, Streptococcus pneumoniae, Listeria sp. and Neisseria sp. The predominant bacterial species isolated was Bacillus sp. 17 (29.8%) while Streptococcus sp., Listeria sp., and Neisseria sp. were the least with 1 (1.8%) each. The prevalence rate of bacteria was higher among the female gender within the age group 21 -30 years. The burden of bacterial infections of the eyes is high. The prevalence and types of bacteria may not be exactly the same in every part of the world. To mitigate the burden of ocular infections, physicians need to comply with etiologic approach of diagnosis and treatment regimen.

scholarly journals Antibacterial and Antibiotic Modifying Potential of Crude Extracts, Fractions, and Compounds from Acacia polyacantha Willd. against MDR Gram-Negative Bacteria

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Flora T. Mambe ◽  
Jean Na-Iya ◽  
Ghislain W. Fotso ◽  
Fred Ashu ◽  
Bathélémy Ngameni ◽  
...  
Keyword(s):  
Oleanolic Acid ◽  
Bacterial Infections ◽  
Efflux Pump ◽  
Synergistic Effects ◽  
Bacterial Species ◽  
Gram Negative Bacteria ◽  
Efflux Pump Inhibitor ◽  
Gram Negative ◽  
Acacia Polyacantha

The present study aimed to assess the in vitro antibacterial and antibiotic modifying activities of methanol extracts prepared from the leaf (APL) and bark (APB) of Acacia polyacantha, fractions (APLa-d) and compounds isolated from APL against a panel of multidrug resistant (MDR) Gram-negative bacteria. Leaf extract was subjected to column chromatography for compounds isolation; antibacterial assays were performed on samples alone and with an efflux pump inhibitor (EPI), respectively, and several antibiotics on the tested bacteria. The phytochemical investigation of APL led to the isolation of stigmasterol (1), β-amyrin (2), 3-O-β-D-glucopyranosylstigmasterol (3), 3-O-methyl-D-chiro-inositol (4), epicatechin (5), quercetin-3-O-glucoside (6), 3-O-[β-D-xylopyranosyl-(1→4)-β-D-galactopyranosyl]-oleanolic acid (7), and 3-O-[β-galactopyranosyl-(1→4)-β-D-galactopyranosyl]-oleanolic acid (8). APL and APB had minimal inhibitory concentration (MIC) values ≤ 1024 μg/mL on 73.3% and 46.7% of the tested bacteria, respectively. APLb and APLd were effective against 88.9% of tested bacterial species with compound 8 showing the highest activity inhibiting 88.9% of tested bacteria. The EPI, phenylalanine-arginine-β-naphthylamide (PAßN), strongly improved the activity of APL, APLb, APLd, and compound 8 on all tested bacteria. Synergistic effects were obtained when APL and compounds 7 and 8 were combined with erythromycin (ERY), gentamycin (GEN), ciprofloxacin (CIP), and norfloxacin (NOR). The present study demonstrates the antibacterial potential of Acacia polyacantha and its constituents to combat bacterial infections alone or in combination with EPI.

scholarly journals Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

2020 ◽  
pp. 68-73
Keyword(s):  
Developing Countries ◽  
Culture Media ◽  
Bacterial Species ◽  
Positive Culture ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Ear Infection ◽  
Pseudomonas Spp ◽  
Multiple Drugs ◽  
Klebsiella Spp

Children are more susceptible to ear infection than adults worldwide, especially in developing countries. In Bangladesh like other developing countries antibiotics is a common choice of treatment in an ear infection. This study was sought to determine causative agents both in right and left ear infection and their antibacterial susceptibility pattern from patients with an ear infection. Specimens of ear discharge were collected aseptically using a sterile swab and cultured on MacConkey’s agar, blood agar, and chocolate agar. After inoculating on appropriate culture media bacterial isolates were identified by their colony morphology, culture characteristics, Gram reaction, and biochemical tests. In this study, a total of 70 positive cases of patients were included. Among these 27 (38.6%) were male, and 43(61.4%) were female. The prevalence of ear infection was significantly high in females (61.4%) than males (38.6%). The predominant bacterial isolates from positive culture were Staphylococcus aureus 43(61.4%), followed by Pseudomonas spp., 13 (18.6%), Streptococcus spp., 8(11.4%), Proteus spp., 5(7.2%), and Klebsiella spp., 1(1.4%). The antibacterial agent like Amikacin and Gentamicin showed a high level of antibacterial effect on all identified bacterial isolates. On the other hand, 98.6% of isolates were found highly resistant to Co-Trimoxazole and Flucloxacillin. Moreover, Streptococcus spp., Proteus spp., Klebsiella spp., and Pseudomonas spp., were highly resistant to multiple drugs (more than 4). A high degree of antibiotic resistance was observed among most of the drugs used in this study. However, Amikacin and Gentamicin were highly effective against the isolated bacterial species. Therefore, culture and susceptibility tests are vital for the appropriate treatment of ear infection.

Activité antimicrobienne d'antioxydants phénoliques

1978 ◽  
Vol 24 (11) ◽  
pp. 1306-1320 ◽  
Author(s):  
Pierre Turcotte ◽  
Samir A. Saheb
Keyword(s):  
Culture Media ◽  
Osmotic Shock ◽  
Bacterial Species ◽  
Butylated Hydroxytoluene ◽  
In Vitro Assays ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
The Family ◽  
Increased Sensitivity

The antimicrobial activity of three antioxydants, butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA) and ethoxyquin (ETO) was studied. In vitro assays showed that when these antioxydants are added to the culture media at concentrations lower or equal to that used in nutrition, they inhibit or decrease the growth of certain microorganisms. BHT showed the most marked effect, affecting Gram-positive bacteria at a higher degree than the Gram-negative bacteria belonging to the family Enterobacteriaceae. Inactivation study of different bacterial species by BHT revealed differences in sensitivity among a single genus and between strains of the same species. The association of ETO with BHT results in an increase of the inhibitory activity. The increased sensitivity to BHT resulting from the osmotic shock of Escherichia coli cells suggests that the resistance to BHT of the Gram-negative bacteria belonging to the family Enterobacteriaceae might be due in part to the structure of their cell wall.

scholarly journals Prevalence and Antibiotic Sensitivity pattern of Bacterial Isolates from Catheterized UTI Patients: A Local Study

2021 ◽  
Vol 2 (2) ◽  
pp. 229-235
Author(s):  
Iqra Arooj ◽  
Alishba Sehar ◽  
Asghar Javaid
Keyword(s):  
Bacterial Infections ◽  
Bacterial Species ◽  
Local Population ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Gram Positive ◽  
Local Study ◽  
Gram Negative ◽  
Sensitivity Pattern ◽  
Tract Infections

Prevalence and multidrug resistance among bacteria in catheter-associated urinary tract infections (CAUTIs) has been on the rise in recent times. Hence, the prevalence rate and antibacterial susceptibility of bacteria in CAUTIs in ICU patients was evaluated. A total of 120 patients admitted to the ICU of Nishtar Hospital, Multan, were recruited for this study. Both gram-positive and gram-negative bacterial isolates were characterized based on biochemical tests including catalase test, oxidase test, indole test, TSI test, citrate test, coagulase test and growth on 6.5% NaCl agar. The prevalence of bacterial species was Escherichia coli (32%), Staphylococcus aureus (26%), Pseudomonas spp. (18%), Proteus spp. (14%) and Enterococcus spp. (2%). A considerable degree of resistance against commonly prescribed antibiotics was observed. Gram negative bacteria showed resistance to ciprofloxacin, piperacillin-tazobactam and amikacin as well as susceptibility to imipenem, tigecycline and polymixin. Gram positive bacteria showed resistance to antibiotics such as piperacillin-tazobactam, ampicillin, gentamicin, oxacillin and ceftazidime suggesting the ineffectiveness of these antibiotics for treating bacterial infections among CAUTI patients and demonstrating the latest trends in antimicrobial drug resistance profile in local population.

scholarly journals Occurrence of Gram-negative bacteria in cockroaches obtained from different areas of Dhaka city

2017 ◽  
Vol 44 (2) ◽  
pp. 229-236
Author(s):  
Tangin Akter ◽  
Sangita Ahmed ◽  
Hasanuzzaman
Keyword(s):  
Periplaneta Americana ◽  
Alimentary Tract ◽  
External Surface ◽  
Bacterial Species ◽  
Gram Negative Bacteria ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Pseudomonas Spp ◽  
Klebsiella Spp

The presence of Gram-negative bacteria in cuticle and alimentary tract of cockroaches collected from four selected areas of Dhaka city was investigated. Using detection key, a total of 12 cockroaches were identified as Periplaneta americana (58.33%), Blattella germanica (33.33%) and Blatta orientalis (8.33%). From these three species 54 different bacterial isolates were obtained. Out of them, 33 were obtained from P. americana (14 from external and 19 from gut), 9 from B. orientalis (5 from external and 4 from gut) and 12 from B. germanica (5 from external and 7 from gut). Bacterial isolates obtained from external surface of the cockroaches belonged to four different Gram negative bacterial species (Klebsiella spp., Pseudomonas spp., Enterobacter spp. and E. coli), six bacteria have been obtained from alimentary tract (Klebsiella spp, Pseudomonas spp., Enterobacter spp., Escherichia coli, Salmonella spp. and Shigella spp.). Pseudomonas spp. were the most predominant bacteria isolated from external surface (33.34%) while Klebsiella spp. were found to dominate in alimentary tract (23.33%). P. americana carried the highest number (33) of bacterial isolates and B. orientalis carried the minimum (9). This study shows that cockroaches carry several bacteria on their external body surface and alimentary tract and can play a role in bacterial transmission to humans. Therefore, the existence of cockroaches in human dwellings is a serious public health problem.Bangladesh J. Zool. 44(2): 229-236, 2016

Characterizing and Overcoming Resistance to Aminomethylspectinomycins in Gram-negative Bacteria

2021 ◽  
Author(s):  
◽  
Nisha Das ◽  
Keyword(s):  
Small Molecule ◽  
Legionella Pneumophila ◽  
Bacterial Infections ◽  
Enzyme Assay ◽  
Bacterial Species ◽  
Enteric Bacteria ◽  
Response Analysis ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli

Spectinomycin (SPC) is a broad-spectrum aminocyclitol antibiotic. Its use in agriculture has led to widespread resistance in enteric bacteria, necessitating the development of more effective analogs. Aminomethyl spectinomycins (amSPC) are modified spectinomycins with increased potency against many bacterial species. These species include Legionella pneumophila, which harbors a chromosomally encoded aminoglycoside modifying enzyme (AME). In this study, we follow up on this observation and examine the extent to which the amSPCs are substrates for AMEs through adenylation (ANTs) and phosphorylation (APH). APH(9)-Ia and ANT(3")(9) were expressed in E. coli BL21(DE3) and purified using the Ni-affinity chromatography. The ability of AMEs to modify and inactivate amSPCs has been examined by two unique biochemical assays, including an agar-based enzyme assay. Binding of APH (9)-Ia and ANT (3")(9) to spectinomycin and amSPCs has been studied using Thermal Denaturation assay and MicroScale Thermophoresis (MST). The microbiological role of these enzymes has been examined by minimum inhibitory concentration (MIC) shifts using an arabinose inducible expression of APH (9)-Ia and ANT (3")(9) in E.coli K12 and JW ΔtolC strains. Our agar-based enzyme assay shows the inactivation of spectinomycin by APH(9)-Ia. Phosphorylated spectinomycin and adenylated spectinomycin products upon incubation with APH(9)-Ia and ANT(3",9), respectively, have been identified using MALDI-MS. APH(9)-Ia induction studies in E. coli tolC knock-out strains reveal a MIC increase against spectinomycin in the presence of 2% arabinose compared to no shift with amSPCs. ANT (3")(9) showed an increase in MIC against spectinomycin as well as amSPCs. In conclusion, amSPCs are not inactivated by APH (9)-Ia in vivo but are inactivated by ANT (3")(9). Most Gram-negative bacteria isolated in clinics possess one or more AMEs. By overcoming modification by AMEs, amSPCs can be a valuable tool in overcoming resistance in Gram-negative bacterial infections. We also conducted a high throughput screen of a polar small molecule library against two multi-drug resistant clinical isolates of Escherichia coli that encode aminoglycoside modifying enzyme for small molecule potentiators of amSPCs to yield 12 possible potentiating molecules that have been confirmed by dose-response analysis. Future work as a continuation of this project will involve further analysis of any existing synergy between the potentiating molecules and amSPCs and target validation of these potentiators.

scholarly journals The Effect of Aqueous and Alcoholic Extracts of Punica granatum L. Pericarp on Hemolysin Production of several Bacterial species

2010 ◽  
Vol 7 (1) ◽  
pp. 309-316
Author(s):  
Baghdad Science Journal
Keyword(s):  
Inhibitory Concentration ◽  
Bacterial Species ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Punica Granatum L

Four local hemolysin producer bacterial isolates were selected, tow of them gram negative bacteria (Escherichia coli ,Pseudomonas aeruginosa ) and the other two were gram positive bacteria (Staphylococcus aureus , Bacillus cereus ). Minimum inhibitory concentration of the aqueous and alcoholic extracts of Punica granatum L. pericarp were determined towards the four bacterial isolates ,results obtaind showed that MICs of the aqueous extract were 200 mg/ml for E .coli and P. aeruginosa isolates while were 5 mg/ml and 2 mg/ml for B. cereus, S. aureus , respectively The MICs for the ethanolic extract were 50 mg/ml , 20 mg/ml ,1 mg/ml ,0.5 mg/ml for E. coli ,P. aeruginosa ,B. cereus ,S. aureus , respectively. The effect of Sub-MICs of the aqueous and alcoholic extracts on hemolysin production was investigated , both extracts had a suppressing effect on hemolysin production by E. coli ,P. aeruginosa ,while both extract had an inhibitory effect on hemolysin production by S. aureus and B . cereus isolates

Similarities between skin culturable bacterial species of pool frogs (Pelophylax lessonae) and their habitat

2021 ◽  
Vol 24 (1) ◽  
pp. 159-163
Author(s):  
F. P. Nocera ◽  
A. De Filippis ◽  
N. Piscopo ◽  
L. Esposito ◽  
L. De Martino
Keyword(s):  
Bacterial Species ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Almost All

The aim of the present study was to investigate the culturable microflora of pool frogs (Pelophylax lessonae) and their belonging aquatic environment. A total of 60 samples (56 frog cutaneous swabs, 4 water samples) were inoculated onto different selective and differential agar plates to isolate Gram-positive and Gram-negative bacteria or yeasts. Microbial investigation of the water hosting frogs was also performed. Isolates were identified by API system and their antibiotic resistance profiles were evaluated by disk diffusion method on Mueller Hinton agar plates. Aeromonas hydrophila and Enterococcus durans were detected in almost all collected samples. Many of the bacterial isolates showed multidrug-resistant profiles. Importantly, this study highlights that skin frog microbiota is correlated to the belonging environment, and, moreover, some isolated bacterial strains resulted to be of interest in animal and public health, since the park was frequented by visitors of all ages.

Dual stain kit for the microscopic gram classification of ocular infection bacteria

1993 ◽  
Vol 51 ◽  
pp. 248-249
Author(s):  
Jacob S. Hanker ◽  
Dale N. Holdren ◽  
Kenneth L. Cohen ◽  
Beverly L. Giammara
Keyword(s):  
Contact Lenses ◽  
Ocular Infection ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Gram Staining ◽  
Ocular Infections ◽  
Different Types ◽  
Culture Positive ◽  
Increased Susceptibility

Keratitis and conjunctivitis (infections of the cornea or conjunctiva) are ocular infections caused by various bacteria, fungi, viruses or parasites; bacteria, however, are usually prominent. Systemic conditions such as alcoholism, diabetes, debilitating disease, AIDS and immunosuppressive therapy can lead to increased susceptibility but trauma and contact lens use are very important factors. Gram-negative bacteria are most frequently cultured in these situations and Pseudomonas aeruginosa is most usually isolated from culture-positive ulcers of patients using contact lenses. Smears for staining can be obtained with a special swab or spatula and Gram staining frequently guides choice of a therapeutic rinse prior to the report of the culture results upon which specific antibiotic therapy is based. In some cases staining of the direct smear may be diagnostic in situations where the culture will not grow. In these cases different types of stains occasionally assist in guiding therapy.

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

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