scholarly journals PENGARUH PEMBERIAN EKSTRAK KULIT BATANG JALOH TERHADAP GAMBARAN MIKROSKOPIS GINJAL TIKUS PUTIH (Rattus novergicus) YANG DIINFEKSI (Trypanosoma evansi The Effect of Wilow Bark Ekstract Administration on Microscopic Feature of Male Rats (Rattus novergicus) Kidney Infected with Trypanosoma evansi)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Nurdiniyah Nurdiniyah ◽  
Nazaruddin Nazaruddin ◽  
Sugito Sugito ◽  
M Nur Salim ◽  
Yudha Fahrimal ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Histopathological Examination ◽  
Kidney Tissue ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Bark Extract ◽  
Control Group ◽  
Willow Bark ◽  
Microscopic Feature ◽  
Group 2

The aims of this study was to examine the ability of willow bark extract to prevent kidney tissue damage from T. evansi infection. This study used 25 rats which were randomly divided into 5 treatment groups, each groups consisted of 5 rats. Rats in control group (K0) were not infected with T. evansi and without administration of willow bark extract. Group 1 (P1) was infected with 103/0,3 ml of T. evansi without willow bark extract administration. Group 2, 3, and 4 were infected with 103/0.3 ml T. evansi and received willow bark extract at dose of 30, 45, and 60 mg/kg bw, respectively. Willow bark extract were administered for 3 days consecutively. On day 4, the rats were sacrificed using chloroform and the kidneys were collected for histopathological examination. Data were analyzed descriptively. The results showed that the administration of willow bark extract at doses of 30 and 45 mg/kg bw were able to prevent damage of tubule cells and glomerular renal, while the administration of 60 mg/kg bw resulted in kidney damage, indicated by necrosis in tubular glomerular cells. It could be concluded that willow bark extract at doses of 30 and 45 mg/kg bw were effective to prevent tissue damage in kidneys.Key words: kidneys, willow, Trypanosoma evansi

scholarly journals The Effect of Wilow Bark Ekstract Administration on Microscopic Feature of Male Rats (Rattus novergicus) Kidney Infected with Trypanosoma evansi

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Nurdiniyah Nurdiniyah ◽  
Nazaruddin Nazaruddin ◽  
Sugito Sugito ◽  
M. Nur Salim ◽  
Yudha Fahrimal ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Histopathological Examination ◽  
Kidney Tissue ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Bark Extract ◽  
Control Group ◽  
Willow Bark ◽  
Microscopic Feature ◽  
Group 2

The aims of this study was to examine the ability of willow bark extract to prevent the kidney tissue damage from T. evansi infection. This study used 25 rats which were randomly divided into 5 treatment groups, each groups consisted of 5 rats. Rats in control group (K0) were not infected with T. Evansi and without administration of willow bark extract. Group 1 (P1) was infected with 103/0,3 ml of T. evansi without willow bark extract administration. Group 2, 3, and 4 were infected with 103/0.3 ml T. evansi and received willow bark extract at dose of 30, 45, and 60 mg/kg bw, respectively. Willow bark extract were administered for 3 days consecutively. On day 4, the rats were sacrificed using chloroform and the kidneys were collected for histopathological examination. Data were analyzed descriptively. The results showed that administration of willow bark extract at doses of 30 and 45 mg/kg bw were able to prevent damage of tubule cells and glomerular renal, while administration of 60 mg/kg bw caused kidney damage, indicated with necrosis in tubular glomerular cells. It could be concluded that the willow bark extract at doses of 30 and 45 mg/kg Bw were effective to prevent tissue damage in kidneys.  Key words: kidneys, willow, Trypanosoma evansi

scholarly journals GAMBARAN HISTOPATOLOGIS LIMPA TIKUS PUTIH (Rattus novergicus) JANTAN DIINFEKSI Trypanosoma evansi DAN DIBERI EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Robx) (Histopathological finding of Rat (Rattus novergicus) Spleen Infected with Trypanosoma evansi and Treated with Willow Bark (Salix tetrasperma Roxb) Extract)

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Elsa Mariane Ramadani ◽  
Hamdani Budiman ◽  
Sugito Sugito ◽  
Ummu Balqis ◽  
Muhammad Hambal ◽  
...  
Keyword(s):  
Trypanosoma Evansi ◽  
Male Rats ◽  
Histopathological Finding ◽  
Bark Extract ◽  
Control Group ◽  
White Pulp ◽  
Treatment Groups ◽  
Group I ◽  
Normal Spleen ◽  
Willow Bark

The aim of this research was to determine the histopathological finding of rat (Rattus novergicus) spleen infected with Trypanosoma evansi and treated with willow bark (Salix tetrasperma Roxb) extract.This research used 25 male rats divided into four treatment groups and fed ad libitum. Group 0 (K0) was control group, group I (K1) was infected with 103/0.3 ml. T. evansi, group II (K2), III (K3), and IV (K4) were infected with103/0.3 ml T. evansi and treated with willow bark extract 30, 45, and 60 mg/kg bw, respectively. All groups were treated for 3 consecutive days orally using a stomach tube. On day four, rats were euthanized using chloroform and necropsied, then spleen were collected for histophatological examination. The results showed that all spleen on group K1, K2, K3, and K4 showed hyperplasia of white pulp, hemosiderin, and necrosis with different severity levels compared to normal spleen structure. In conclusion, the administration of willow bark extract at the dose of 30 mg/kg bw has a potency to prevent spleen from T. evansi infection. However, the higher the dose of willow bark extract the higher the level of spleen damage.Key words: spleen, T. evansi, willow bark extract

INVESTIGATION OF THE EFFECT OF BLACK SEED OIL ON DIABETES-INDUCED - TESTICULAR DAMAGE: A HISTOPATHOLOGICAL STUDY

2021 ◽  
pp. 29-31
Author(s):  
Seval Kaya ◽  
Yusuf Nergiz ◽  
Firat Asir
Keyword(s):  
Tissue Damage ◽  
Seed Oil ◽  
Histopathological Examination ◽  
Male Rats ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Histopathological Study ◽  
Diabetes Group ◽  
Black Seed ◽  
Black Seed Oil

In this study, it was aimed to investigate the protective effect of black seed oil against testicular tissue damage in diabetic rats. A total of 18 male rats were divided into 3 groups, including 6 rats in each group.Groups; control (n=6), diabetes (n=6), diabetes + black seed oil (n=6). A single dose of 45 mg / kg streptozocine (STZ) was injected intraperitoneally to induce diabetes. Diabetes + Black seed oil group: For 56 days, 2.5 ml / kg of black seed oil was administered orally to rats.The rats were sacriced at the end of 56 days. Testicular tissues were taken for routine parafn tissue processing for histopathological examination. Parafn sections were stained with Hematoxylin-Eosin and PAS and examined under a light microscope. Atrophy and degeneration were observed in the seminiferous tubules of diabetic group. Histology of black seed oil group sections were similar to that of control group. A signicant difference was found between the black seed oil group and the diabetes group in terms of blood glucose values. As a result, we think that Black Seed Oil ameliorates to the tissue damage caused by diabetes and the decrease in blood sugar value.

scholarly journals Speman®, A Proprietary Ayurvedic Formulation, Reverses Cyclophosphamide-Induced Oligospermia In Rats.

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
Mohd. Azeemuddin Mukram ◽  
Mohamed Rafiq ◽  
Suryakant D. Anturlikar ◽  
Pralhad S. Patki
Keyword(s):  
Body Weight ◽  
Histopathological Examination ◽  
Sperm Count ◽  
Reproductive Organs ◽  
Positive Control ◽  
Male Rats ◽  
Control Group ◽  
Once Daily ◽  
Respective Treatment ◽  
Group 2

Background: This investigation was aimed to evaluate the effect of Speman®, a well known ayurvedic proprietary preparation, in an experimental model of cyclophosphamide-(CP) induced oligospermia in rats.Materials and Methods: Thirty male rats were randomized in to five, equally-sized groups. Rats in group 1 served as a normal control; group 2 served as an untreated positive control; groups 3, 4, 5 received  Speman® granules  at doses of 300, 600, and 900mg/kg body weight p.o. respectively, once daily for 13 days. On day four, one hour after the respective treatment, oligospermia was induced by administering a single dose of CP (100mg/kg body weight p.o.)  to all the groups except group1. At the end of the study period the rats were euthanised and accessory reproductive organs were weighed and subjected to histopathological examination. The semen samples were subject to enumeration of sperms.  Weight of the reproductive organs, histopathological examination of the tissues, and sperm count were the parameters studied to understand the effect of Speman® on rats with CP-induced oligospermia.Results: Changes that occurred due to the administration of CP at a dose of 100 mg/kg body weight were dose dependently reversed with Speman® at a dose of 300, 600, and 900 mg/kg body weight. There was a statistically significant increase in sperm count and the weight of the seminal vesicle, epididymis, and prostate.Conclusion: Findings of this investigation indicate that Speman® dose dependently reversed the CP-induced derangement of various parameters pertaining to the reproductive system.  This could explain the total beneficial actions of Speman® reported in several other clinical trials.

scholarly journals 26. GAMBARAN HISTOPATOLOGIS GINJAL TIKUS PUTIH (Rattus novergicus) JANTAN YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERI EKSTRAK DAUN SERNAI (Wedelia biflora) (Histopathology of Male Rat (Rattus novergicus) Kidney Infected with Trypanosoma evansi and Treated with Sernai Leaves Extract (Wedelia biflora))

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Yudha Fahrimal ◽  
Rahmiwati R ◽  
Dwinna Aliza
Keyword(s):  
Untreated Control ◽  
Histopathological Examination ◽  
Rat Kidney ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Male Rat ◽  
Control Group ◽  
Untreated Control Group ◽  
Stomach Tube ◽  
Treatment Groups

This research was aimed to study histopathology of rat kidney infected with Trypanosoma evansi after administration of sernai leaves extract (Wedelia bifolora). The samples used were kidneys of 25 male rats strain Wistar which were equally divided into 5 treatment groups. Group P1 was uninfected and untreated control, group P2 was infected with 1x103 T. evansi but not sernai leaves extract, rats from groups P3, P4, and P5 were infected with 1x103 T. evansi and given sernai leaves extract with the dose of 30 mg/kg bw, 45 mg/kg bw, and 60 mg/kg bw respectively. The sernai extract was administered orally using a stomach tube for 3 consecutive days. The next day after last treatment, all rats were sacrificed and necropsied. Kidneys were collected for histopathological examination such as degeneration and necrosis of tubules cells, convoluted contortus duct lumen, and adhesion and atrophy of glomerulus. Sernai leaves extract with the dose of 45 mg/kg bw was the most effective dose to prevent kidney damage due to T. evansi infection.

scholarly journals GAMBARAN HISTOPATOLOGIS HATI TIKUS (Rattus novergicus) YANG DIINFEKSI Trypanosoma evansi SETELAH PEMBERIAN EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Roxb) (Histopathological Changes of Rat (Rattus novergicus) Liver Infected with Trypanosoma evansi and Treated with Willow Tree Bark Extract (Salix tetrasperma Roxb))

2015 ◽  
Vol 9 (2) ◽  
Author(s):  
Mulya Fahmi ◽  
Yudha Fahrimal ◽  
Dwinna Aliza ◽  
Siti Aisyah ◽  
Hamdani Budiman ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Tree Bark ◽  
Male Rat ◽  
Bark Extract ◽  
Control Group ◽  
Histopathological Changes ◽  
Hydropic Degeneration ◽  
Willow Tree

This research aimed to determine the effect of willow (Salix tetrasperma Roxb) tree bark extract on histopathological changes of liver of rat (Rattus novergicus) infected with Trypanosoma evansi. Liver of 25 male rat, Wistar strain, were used in this study and all rat were fed with commercial feed ad libitum. This research used completely randomized design (CRD) with 5 treatments, each treatment consisted of 5 replicates. Treatment I was negative control group without any treatment, treatment II were only infected with 103 T. evansi, treatment III, IV, and V were infected with 103 T. evansi and treated with willow tree bark extract with the dose of 30, 45, and 60 mg/kg bw, respectively. The administration of willow tree bark extract was carried out for 3 consecutive days orally using a stomach tube. All rats were sacrificed and necropsied then liver were collected for histopathological examination. Percentage of histophatological changes found in hepatocytes of rat infected with T. evansi after the administration of willow tree bark extract in group P1, P2, P3, P4, and P5 were: normal hepatocytes 96.28%; 0.00%; 8.06%; 40.61%; and 0.00%; hydropic degeneration 0.00%, 44.98%, 58.00%, 38.29%, and 35.04%; fatty degeneration 0.00%, 0.00%, 0.00%, 0.00%, and 8.76%; necrosis 3.72%, 55.02%, 33.94%, 21.10%, and 56.20, respectively. In conclusion, the administration of willow tree bark extract is able to protect rat’s liver from T. evansi infection and the dose of 45 mg/kg bw is more effective than 30 mg/kg bw but the dose of 60 mg/kg bw of willow tree bark extract worsen the liver damage.Key words: histopathology, liver, T. evansi, willow

scholarly journals The Effect of Experimental Escherichia coli Infection on Some Blood Parameters and Histological Changes in Male Rats

2011 ◽  
Vol 35 (2) ◽  
pp. 22-27
Author(s):  
Hayder AL – Zamely
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histopathological Examination ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Infected Group ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules

This study was carried out to investigate the effect of experimental infection by Escherichia coli on some blood parameters and histopathological changes in male rats to reach this objective twenty male rats (Rattus norvigicus) were divided into two groups, housed under same conditions of water, feed, temperature, light and humidity. The first one served as control group, while the second was experimentally infected with Ecoli by administration of 2ml / rat of (1×109) colony forming unit/ml of E-coli. Five days later blood were obtained from animals by cardiac puncture to measure the total and differential white blood cells count hemoglobin concentration packed cells volume red blood cells count, and thrombocytes count. One animal from each group was killed and histopathological examination was done for intestine kidney liver and spleen to detect the effect of infection on these organs. The results revealed a significant increase in total leukocytes count (P<005) in infected group while the differential count showed a significant increase in neutrophils and significant decrease in lymphocytes (P< 0.05) in same group. There were a significant decrease in hemoglobin concentration packed cell volume and non significant decrease in red blood cells count. While thrombocytes count showed a significant decrease in infected group compared with control group (P<0.05). The histopathological examination of infected male rats intestine was demonstrated mild diffuse inflammation which include increased numbers of neutrophile in tissue intraepithelial lymphocytes and goblet cells In kidney renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. In liver hepatocytes were swollen with decreased sinusoidal spaces and widely distributed necrotic foci were seen. There is extensive splenic necrosis with infiltration of leukocytes from white pulp to red pulp of spleen.

scholarly journals The Effect of Zinc and Melatonin Supplementation on Lipid Peroxidation of Various Tissues of Rats with by DMBA-Induced Breast Cancer

2020 ◽  
Vol 11 (1) ◽  
pp. 7580-7588
Keyword(s):  
Breast Cancer ◽  
Lipid Peroxidation ◽  
Kidney Tissue ◽  
Female Rats ◽  
Control Group ◽  
Tissue Samples ◽  
Cancer Group ◽  
Group 5 ◽  
Group 2 ◽  
Group Control Group

The purpose of this study was to investigate the effects of zinc and melatonin administration on lipid peroxidation in various tissues in DMBA-induced breast cancer in female rats. A total of 42 recently weaned Wistar rats were divided into 5 groups in the study: Group 1 (Control), Group 2 (DMBA Control), Group 3 (DMBA+Zinc), Group 4 (DMBA+Melatonin), Group 5 (DMBA+Melatonin, and Zinc). MDA (malondialdehyde) and GSH (glutathione) levels were determined via the spectrophotometric method in the lung, liver, spleen, pancreas, and kidney tissue samples taken from experimental animals. The highest lung, liver, spleen, pancreas, and kidney tissue MDA levels were obtained in the DMBA-induced breast cancer group control group (G2) (p<0.05). MDA levels in DMBA+Zinc (G3), DMBA+Melatonin (G4), and DMBA+Melatonin and Zinc (G5) were significantly lower than group 2 (p<0.05). Similarly, lung, liver, spleen, pancreas, and kidney tissue GSH levels of DMBA+Zinc (G3), DMBA+Melatonin (G4), and DMBA+Melatonin and Zinc (G5) were significantly higher than that of Group 2 (p<0.05). The findings of the study indicated that increased lung, liver, spleen, pancreas, and kidney damage in DMBA-induced breast cancer is suppressed with the supplementation of zinc, melatonin, and combined zinc and melatonin.

scholarly journals Mechanism of Pancreatic Islet Number Improvement on Hyperglycemic Wistar Rats (Rattus norvegicus) After Golden Sea Cucumber (Stichopus hermanii) Extract Administration

2020 ◽  
Vol 9 (3S) ◽  
pp. 320-324
Keyword(s):  
Blood Sugar ◽  
Pancreatic Islet ◽  
Blood Sugar Level ◽  
Sea Cucumber ◽  
Histopathological Examination ◽  
Ethanol Extract ◽  
Male Rats ◽  
Laboratory Research ◽  
Control Group ◽  
Sugar Levels

Diabetes mellitus constitutes a metabolic disorder indicated by a decrease in insulin secretion, insulin insensitivity, or a combination of both. Golden sea cucumber (Stichopus hermanii) is a natural resource of Indonesian waters with benefit for reducing blood sugar levels. This research aimed to determine the anti-hyperglycemic mechanism of golden sea cucumber.This study used a experimental laboratory research with Post-test Only Control Group. The sample of this study consisted of 25 male rats aged 60-70 days, divided into 5 groups: K(-), K(+), P1, P2, and P3 randomly. Rats from K(+), P1, P2, and P3 groups were injected with streptozotocin (STZ) with a dose of 50 mg/kg BW. Treatment was administered for 21 days. K(-) and K(+) groups were fed normally while P1, P2 and P3 groups were given golden sea cucumber 80% ethanol extract with a dose of 4,25 mg/kgBW/day, 8,50 mg/kgBW/day and 12,75 mg/kgBW/day respectively. On the 22nd day, fasting blood sugar level was measured and the number of pancreatic islet was counted with histopathological examination. The administration of golden sea cucumber (Stichopus hermanii) extract on rats with hyperglycemia does not reduce blood sugar level, but the extract administration increases the number of pancreatic islet. Golden sea cucumber (Stichopus hermanni) extract may improve the number of pancreatic islet and prevent the progressive damage of pancreatic islet.C

scholarly journals Comparative Assessment on Mechanism Underlying Renal Toxicity of Commercial Formulation of Roundup Herbicide and Glyphosate Alone in Male Albino Rat

2018 ◽  
Vol 37 (4) ◽  
pp. 285-295 ◽  
Author(s):  
Gabriel A. Dedeke ◽  
Folarin O. Owagboriaye ◽  
Kehinde O. Ademolu ◽  
Olanrewaju O. Olujimi ◽  
Adeyinka A. Aladesida
Keyword(s):  
Oxidative Stress ◽  
Active Ingredient ◽  
High Performance ◽  
Kidney Tissue ◽  
Male Rats ◽  
Control Group ◽  
Albino Rat ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

There have been major concerns that the nephrotoxicity of commercial formulations of Roundup herbicide is due to the active ingredient glyphosate. We therefore investigated and compared the mechanisms underlining the nephrotoxicity of Roundup herbicide and glyphosate alone in rat. Fifty-six adult male rats randomized into 7 groups of 8 rats per group were exposed to Roundup formulation and glyphosate alone daily by gavage at 3.6, 50.4, and 248.4 mg/kg body weight (bw) of glyphosate concentrations for 12 weeks with distilled water administered to the control group. Kidney biomarker (serum urea and creatinine, plasma cystatin-C, and neutrophil gelatinase-associated lipocalin), oxidative stress indices in the kidney tissue, activities of kidney membrane-bound enzymes (Mg-adenosine triphosphatase [ATPase], Ca-ATPase, Na/K-ATPase, and total ATPase), and histopathological changes in the kidney were monitored. Glyphosate concentration in the kidney was quantified by high-performance liquid chromatography with ultraviolet detection. Significant ( P < 0.05) alterations in the levels of the kidney biomarker, oxidative stress markers, and membrane-bound enzymes were observed in the rats exposed to Roundup compared to the rats exposed to glyphosate alone. Rats exposed to Roundup accumulated more glyphosate residue in their kidney tissue. Severe histopathological lesions were only seen in the kidneys of rats exposed to Roundup. The nephrotoxicity observed cannot be due to the active ingredient in the Roundup formulation, as glyphosate alone has virtually no effect on the renal function of the exposed animals. Therefore, the general claim attributing nephrotoxicity of a glyphosate-based herbicide to its active ingredient should be discouraged.

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