iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

10.21203/rs.2.20012/v2 ◽

2020 ◽

Author(s):

Wen Song ◽

Fengxian Tang ◽

Wenchao Cai ◽

Qin Zhang ◽

Fake Zhou ◽

...

Keyword(s):

Cold Stress ◽

Cold Tolerance ◽

Proteomic Analysis ◽

Molecular Mechanisms ◽

Treatment Time ◽

Chilling Injury ◽

Cold Treatment ◽

Cold Response ◽

Low Temperature Storage ◽

Metabolic Analysis

Abstract Background: During the low temperature storage, cantaloupe is susceptible to the cold stress, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of cold tolerance in cantaloupe, cold-sensitive cultivar Goldqueen-308 (GE) and cold-tolerant cultivar Jiashi-310 (JS) were used for quantitative proteomic analysis with iTRAQ in parallel. Results: In this work, two commercial cultivars were treated at 0.5℃ for 0, 12 and 24 days. Phenotypes assays showed that GE suffered a more severe damage as the cold treatment time extended. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) changed significantly over time in cold-exposed cantaloupe. Comparing with GE, JS responded in a prompter manner in terms of expressing cold-responding proteins during the similarly cold treatment. Furthermore, much more different groups of proteins were mobilized in response to the cold treatment in JS comparing with GE. Metabolic analysis indicated that more amino acids were up-regulated in JS during the early phases of cold stress. This study also identified some DEPs since they were up-regulated in JS or down-regulated in GE in terms of molecular mechanisms, which were mainly related to carbohydrate and energy metabolism, structure proteins, ROS scavening, amino acid metabolic and signaling transduction. Moreover, iTRAQ analysis was confirmed to be reliable via the results of phenotypes assays, metabolic analysis and q-PCR validation. Conclusion: By proteomics information,we found that the prompt response and the significant mobilization of proteins in JS maintained a higher level of cold tolerance, and the delay of cold response in GE could be a critical reason for the severe chilling injury. The candidate proteins we found will be the basis of future studies for further investigations and our findings may help to better understand the novel mechanisms of cold tolerance in cantaloupe.

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iTRAQ-based quantitative proteomics analysis of cantaloupe (Cucumis melo var. saccharinus) after cold storage

10.21203/rs.2.20012/v1 ◽

2020 ◽

Author(s):

Wen Song ◽

Fengxian Tang ◽

Wenchao Cai ◽

Qin Zhang ◽

Fake Zhou ◽

...

Keyword(s):

Cold Stress ◽

Cold Tolerance ◽

Proteomic Analysis ◽

Molecular Mechanisms ◽

Treatment Time ◽

Chilling Injury ◽

Cold Treatment ◽

Cold Response ◽

Low Temperature Storage ◽

Metabolic Analysis

Abstract Background: During the low temperature storage, cantaloupe is susceptible to the cold stress, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of cold tolerance in cantaloupe, cold-sensitive cultivar Goldqueen-308 (GE) and cold-tolerant cultivar Jiashi-310 (JS) were used for quantitative proteomic analysis with iTRAQ in parallel. Results: In this work, two commercial cultivars were treated at 0.5℃ for 0, 12 and 24 days. Phenotypes assays showed that GE suffered a more severe damage as the cold treatment time extended. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) changed significantly over time in cold-exposed cantaloupe. Comparing with GE, JS responded in a prompter manner in terms of expressing cold-responding proteins during the similarly cold treatment. Furthermore, much more different groups of proteins were mobilized in response to the cold treatment in JS comparing with GE. Metabolic analysis indicated that more amino acids were up-regulated in JS during the early phases of cold stress. This study also identified some DEPs since they were up-regulated in JS or down-regulated in GE in terms of molecular mechanisms, which were mainly related to carbohydrate and energy metabolism, structure proteins, ROS scavening, amino acid metabolic and signaling transduction. Moreover, iTRAQ analysis was confirmed to be reliable via the results of phenotypes assays, metabolic analysis and q-PCR validation. Conclusion: By proteomics information,we found that the prompt response and the significant mobilization of proteins in JS maintained a higher level of cold tolerance, and the delay of cold response in GE could be a critical reason for the severe chilling injury. The candidate proteins we found will be the basis of future studies for further investigations and our findings may help to better understand the novel mechanisms of cold tolerance in cantaloupe.

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SSR Mapping of QTLs Conferring Cold Tolerance in an Interspecific Cross of Tomato

International Journal of Genomics ◽

10.1155/2016/3219276 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

Yang Liu ◽

Tengxia Zhou ◽

Haiyan Ge ◽

Wen Pang ◽

Lijie Gao ◽

...

Keyword(s):

Cold Tolerance ◽

Interspecific Cross ◽

Cold Treatment ◽

Chilling Temperature ◽

Plant Trait ◽

Molecular Linkage Map ◽

Cold Tolerant ◽

Cold Sensitive ◽

Fine Position ◽

Simple Sequence

A population of 146 RILs (Recombinant Inbred Line) was derived from the cross between a cold-sensitive cultivatedSolanum lycopersicumL. XF98-7 and a cold-tolerant wildSolanum pimpinellifoliumLA2184. Relative germination ratio (RGR) and chilling index (CI) were used to evaluate the cold tolerance of the parental lines and RILs. It was found that the RGR and CI were significantly different betweenS. lycopersicumXF98-7 andS. pimpinellifoliumLA2184 under cold treatment, indicating that wild species was more adapted to chilling temperature. The continuous and normal distribution of RGR and CI in RIL population suggested that the trait of cold tolerance was a typically quantitative trait controlled by multigenes. The molecular linkage map was constructed by using 120 simple-sequence repeat (SSR) markers, resulting in 15 linkage groups, with a total distance of 256.8 cM and average interval of 2.14 cM. Five QTLs controlling RGR and four QTLs for CI were detected with genetic contribution ranging from 0.95% to 19.55%. Thus, the nine QTLs will provide references for further fine position mapping for cold tolerance. The polymorphic markers could be used as a way of indirectly selecting the plant trait of interest and would promote developing new tomato variety by marker-assisted selection.

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Drosophila beta-Tubulin 97EF is upregulated at low temperature and stabilizes microtubules

10.1101/185827 ◽

2017 ◽

Author(s):

Faina Myachina ◽

Fritz Bosshardt ◽

Johannes Bischof ◽

Moritz Kirschmann ◽

Christian F. Lehner

Keyword(s):

Low Temperature ◽

Low Temperatures ◽

Molecular Mechanisms ◽

Specific Pattern ◽

Beta Tubulin ◽

Tissue Specific ◽

Temperature Range ◽

Microtubule Stability ◽

Cold Sensitive ◽

Summary Statement

Summary statementEctotherms thrive within an often remarkable temperature range. At low temperature, betaTub97EF, a beta-tubulin paralog stabilizing microtubules, is upregulated in a tissue-specific manner in the fly Drosophila melanogaster.AbstractCells in ectotherms function normally within an often wide temperature range. As temperature dependence is not uniform across all the distinct biological processes, acclimation presumably requires complex regulation. The molecular mechanisms coping with the disruptive effects of temperature variation are still poorly understood. Interestingly, one of five different beta-tubulin paralogs, betaTub97EF, was among the genes up-regulated at low temperature in cultured Drosophila cells. As microtubules are known to be cold-sensitive, we analyzed whether betaTub97EF protects microtubules at low temperatures. During development at the optimal temperature (25°C), betaTub97EF was expressed in a tissue-specific pattern primarily in the gut. There, as well as in hemocytes, expression was increased at low temperature (14°C). While betaTub97EF mutants were viable and fertile at 25°C, their sensitivity within the well-tolerated range was slightly enhanced during embryogenesis specifically at low temperatures. Changing beta-tubulin isoform ratios in hemocytes demonstrated that beta-Tubulin 97EF has a pronounced microtubule stabilizing effect. Moreover, betaTub97EF is required for normal microtubule stability in the gut. These results suggest that betaTub97EF up-regulation at low temperature contributes to acclimation by stabilizing microtubules.

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Genome-Wide Analysis of the miRNA–mRNAs Network Involved in Cold Tolerance in Populus simonii × P. nigra

Genes ◽

10.3390/genes10060430 ◽

2019 ◽

Vol 10 (6) ◽

pp. 430 ◽

Cited By ~ 3

Author(s):

Bo Zhou ◽

Yutong Kang ◽

Jingtong Leng ◽

Qijiang Xu

Keyword(s):

Cold Tolerance ◽

High Throughput Sequencing ◽

Expression Patterns ◽

Differential Expression Analysis ◽

Chilling Injury ◽

Cold Treatment ◽

Differentially Expressed ◽

Integrated Analysis ◽

Populus Simonii ◽

Cold Induction

Background: Cold tolerance is important for plants’ geographical distribution and survival in extreme seasonal variations of climate. However, Populus simonii × P. nigra shows wide adaptability and strong cold resistance. Transcriptional and post-transcriptional regulation of cold-responsive genes is crucial for cold tolerance in plants. To understand the roles of regulatory RNAs under cold induction in Populus simonii × P. nigra, we constructed cDNA and small RNA libraries from leaf buds treated or not with −4 °C for 8 h for analysis. Results: Through high-throughput sequencing and differential expression analysis, 61 miRNAs and 1229 DEGs were identified under cold induction condition in Populus simonii × P. nigra. The result showed that miR167a, miR1450, miR319a, miR395b, miR393a-5p, miR408-5p, and miR168a-5p were downregulated, whereas transcription level of miR172a increased under the cold treatment. Thirty-one phased-siRNA were also obtained (reads ≥ 4) and some of them proceeded from TAS3 loci. Analysis of the differentially expressed genes (DEGs) showed that transcription factor genes such as Cluster-15451.2 (putative MYB), Cluster-16493.29872 (putative bZIP), Cluster-16493.29175 (putative SBP), and Cluster-1378.1 (putative ARF) were differentially expressed in cold treated and untreated plantlets of Populus simonii × P. nigra. Integrated analysis of miRNAs and transcriptome showed miR319, miR159, miR167, miR395, miR390, and miR172 and their target genes, including MYB, SBP, bZIP, ARF, LHW, and ATL, were predicted to be involved in ARF pathway, SPL pathway, DnaJ related photosystem II, and LRR receptor kinase, and many of them are known to resist chilling injury. Particularly, a sophisticated regulatory model including miRNAs, phasiRNAs, and targets of them was set up. Conclusions: Integrated analysis of miRNAs and transcriptome uncovered the complicated regulation of the tolerance of cold in Populus simonii × P. nigra. MiRNAs, phasiRNAs, and gene-encoded transcription factors were characterized at a whole genome level and their expression patterns were proved to be complementary. This work lays a foundation for further research of the pathway of sRNAs and regulatory factors involved in cold tolerance.

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Molecular interconversion of cold-sensitive cytosolic 3,3′,5-tri-iodo-l-thyronine-binding proteins from human erythrocytes: effect of cold, heat and pH treatments

Biochemical Journal ◽

10.1042/bj2900579 ◽

1993 ◽

Vol 290 (2) ◽

pp. 579-582 ◽

Cited By ~ 3

Author(s):

A N Fanjul ◽

R N Farías

Keyword(s):

Blood Cells ◽

Low Temperatures ◽

Binding Proteins ◽

Cold Treatment ◽

Rapid Loss ◽

Polymeric Form ◽

Human Red Blood Cells ◽

Similar Association ◽

Cold Sensitive ◽

Inactive Monomer

Cytosolic 3,3′,5-tri-iodo-L-thyronine-binding proteins (CTBP I, II and IV species) from human red blood cells undergo rapid loss of activity at low temperatures. Cold treatment of CTBPs was accompanied by dissociation of the polymeric protein to the 60 kDa inactive monomer. Re-activation of the cold-inactivated CTBP IV by warming resulted in association of the monomer to the active polymeric form. A similar association-dissociation phenomenon was also obtained isothermically, though pH changes. We conclude that CTBP I and CTBP II are polymeric forms of CTBP IV.

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Comparative proteomics analysis reveals the molecular mechanism of enhanced cold tolerance through ROS scavenging in winter rapeseed (Brassica napus L.)

PLoS ONE ◽

10.1371/journal.pone.0243292 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0243292

Author(s):

Wenbo Mi ◽

Zigang Liu ◽

Jiaojiao Jin ◽

Xiaoyun Dong ◽

Chunmei Xu ◽

...

Keyword(s):

Cold Stress ◽

Cold Tolerance ◽

Molecular Mechanisms ◽

Early Stage ◽

Cold Treatment ◽

Ros Scavenging ◽

Brassica Napus L ◽

Membrane Injury ◽

Data Independent Acquisition ◽

Winter Rapeseed

Two winter rapeseed cultivars, “NS” (cold tolerant) and “NF” (cold sensitive), were used to reveal the morphological, physiological, and proteomic characteristics in leaves of plants after treatment at -4°C for 12 h(T1) and 24 h(T2), and at room temperature(T0), to understand the molecular mechanisms of cold tolerance. Antioxidant activity and osmotic adjustment ability were higher, and plasma membrane injury was less obvious, in NS than in NF under cold stress. We detected different abundant proteins (DAPs) related to cold tolerance in winter rapeseed through data-independent acquisition (DIA). Compared with NF, A total of 1,235 and 1,543 DAPs were identified in the NSs under T1 and T2, respectively. Compared with NF, 911 proteins were more abundant in NS only after cold treatment. Some of these proteins were related to ROS scavenging through four metabolic pathways: lysine degradation; phenylalanine, tyrosine, and tryptophan; flavonoid biosynthesis; and ubiquinone and other terpenoid-quinone biosynthesis. Analysis of these proteins in the four candidate pathways revealed that they were rapidly accumulated to quickly enhance ROS scavenging and improve the cold tolerance of NS. These proteins were noticeably more abundant during the early stage of cold stress, which was critical for avoiding ROS damage.

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Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

AoB Plants ◽

10.1093/aobpla/plz045 ◽

2019 ◽

Vol 11 (6) ◽

Cited By ~ 1

Author(s):

Xiaoyan Cai ◽

Richard Odongo Magwanga ◽

Yanchao Xu ◽

Zhongli Zhou ◽

Xingxing Wang ◽

...

Keyword(s):

Cold Stress ◽

Stress Tolerance ◽

Cold Tolerance ◽

Molecular Mechanisms ◽

Chilling Stress ◽

Cold Treatment ◽

Cold Sensitivity ◽

Cotton Species ◽

Wide Range ◽

Gossypium Thurberi

Abstract Low temperature is one of the key environmental stresses that impair plant growth and significantly restricts the productivity and spatial distribution of crop plants. Gossypium thurberi, a wild diploid cotton species, has adapted to a wide range of temperatures and exhibits a better tolerance to chilling stress. Here, we compared phenotypes and physiochemical changes in G. thurberi under cold stress and found this species indeed showed better cold tolerance. Therefore, to understand the molecular mechanisms of the cold tolerance in G. thurberi, we compared transcription changes in leaves of G. thurberi under cold stress by high-throughput transcriptome sequencing. In total, 35 617 unigenes were identified in the whole-genome transcription profile, and 4226 differentially expressed genes (DEGs) were discovered in the leaves upon cold treatment. Gene Ontology (GO) classification analyses showed that the majority of DEGs belonged to categories of signal transduction, transcription factors (TFs) and carbohydrate transport and metabolism. The expression of several cold-responsive genes such as ICE1, CBF4, RAP2-7 and abscisic acid (ABA) biosynthesis genes involved in different signalling pathways were induced after G. thurberi seedlings were exposed to cold stress. Furthermore, cold sensitivity was increased in CBF4 and ICE2 virus-induced gene silencing (VIGS) plants, and high level of malondialdehyde (MDA) showed that the CBF4 and ICE2 silenced plants were under oxidative stress compared to their wild types, which relatively had higher levels of antioxidant enzyme activity, as evident by high levels of proline and superoxide dismutase (SOD) content. In conclusion, our findings reveal a new regulatory network of cold stress response in G. thurberi and broaden our understanding of the cold tolerance mechanism in cotton, which might accelerate functional genomics studies and genetic improvement for cold stress tolerance in cultivated cotton.

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Rootstock-Mediated Transcriptional Changes Associated with Cold Tolerance in Prunus mume Leaves

Horticulturae ◽

10.3390/horticulturae7120572 ◽

2021 ◽

Vol 7 (12) ◽

pp. 572

Author(s):

Faisal Hayat ◽

Chengdong Ma ◽

Shahid Iqbal ◽

Xiao Huang ◽

Ouma Kenneth Omondi ◽

...

Keyword(s):

Oxidative Damage ◽

Cold Stress ◽

Cold Tolerance ◽

Molecular Mechanisms ◽

Mapk Signaling ◽

Differentially Expressed ◽

Prunus Mume ◽

Japanese Apricot ◽

Cold Tolerant ◽

Cold Sensitive

Japanese apricot (Prunus mume) is remarkably valuable for its high ornamental and economic importance due to its distinctive features. Low temperature is a serious environmental constraint for this species, restricting its cultivation and dispersal in the north of China. To address this issue, breeding requires an understanding of the molecular mechanisms underlying responses to cold stress. We examined the leaf physiological and transcriptome profile by RNA sequencing in ‘Bungo’ scion cultivar grafted onto Prunus mume (cold-sensitive) and Prunus armeniaca (cold-tolerant) rootstocks at 4 °C for 0, 6, and 24 h. Our results revealed that the increased MDA concentration in the leaves of P. mume cultivar (cold-sensitive) suggests that cold stress might cause oxidative damage and increased sensitivity. Moreover, the cold-tolerant cultivar (P. armeniaca) considerably enhances the enzyme activities (i.e., SOD, POD, and CAT), as well as osmo-protectants (soluble sugars and proline) compared with sensitive cultivar, which helps plants to withstand oxidative damage caused by cold stress. Additionally, differentially expressed genes were shown to be enriched in plant hormone signal transduction, ribosome, MAPK signaling, and circadian rhythm pathway. After 24 h of cold stress, genes related to PYL4, histidine kinase 1, SAUR36, bHLH130, bHLH123, TIFY 6B-like, WRKY 40, WRKY 57, and 60S acidic ribosomal protein P1 were differentially expressed, implying that these DEGs involved in multiple pathways are involved in cold tolerance in Japanese apricot. This study improved our current understanding of the mechanism of cold tolerance in Japanese apricot, and the findings could be utilized for other related fruit species.

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Reduced Heat Shock Period of `Sharwil' Avocado for Cold Tolerance in Quarantine Cold Treatment

HortScience ◽

10.21273/hortsci.30.5.1052 ◽

1995 ◽

Vol 30 (5) ◽

pp. 1052-1053 ◽

Cited By ~ 7

Author(s):

Kate A. Nishijima ◽

Harvey T. Chan ◽

Suzanne S. Sanxter ◽

Edward S. Linse

Keyword(s):

Heat Shock ◽

Cold Tolerance ◽

Chilling Injury ◽

Cold Treatment ◽

Persea Americana ◽

Shock Period ◽

Heat Pretreatment ◽

Pretreatment Period

A reduced heat shock period for `Sharwil' avocado (Persea americana Mill.) before quarantine cold treatment is described. The shortened heat pretreatment period of 8 to 12 hours, rather than the originally recommended 18 hours at 38C, is effective in reducing chilling injury symptoms when the pulp is at ≤2.2C during 16 days of storage. The reduced durations and the range of pretreatment hours affords packinghouses greater efficiency and more flexibility and will reduce handling costs relative to the longer exposure.

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