scholarly journals Ethanol in Food affects behaviour and HPA axis activity during development in zebrafish larvae Subhead: Ethanol in Food affects Nerves during development in zebrafish larvae

2020 ◽  
Author(s):  
wenxiao du ◽  
Xiaoli Chen ◽  
Zhenjun Zhao ◽  
min shi ◽  
fuhua bian
Keyword(s):  
Locomotor Activity ◽  
Hpa Axis ◽  
Ethanol Exposure ◽  
Enzyme Linked Immunosorbent Assay ◽  
Zebrafish Larvae ◽  
Behavioural Analysis ◽  
Polymerase Chain ◽  
External Stimuli ◽  
Hpa Axis Activity

Abstract Long-term alcohol intake from food can lead to numerous mental disorders in humans, and cause serious problems for governments and families worldwide. However, currently, it is unclear how alcohol affects the hypothalamic–pituitary–adrenal (HPA) axis. In the present study, using zebrafish larvae exposed to 1% ethanol, we made Zebrafish behavioural analysis, samples were collected for the enzyme-linked immunosorbent assay (ELISA) and quantitative real-time polymerase chain reaction (qRT-PCR) experiments,and made statistical analyses at last. we found that ethanol decreases the locomotor activity of zebrafish larvae,showed a more intense reaction to external stimuli,increases the secretion of HPA axis hormones in zebrafish larvae,influences the secretion of neurotransmitters,alters key gene expression during neurotransmitter metabolism. Ethanol exposure reduced zebrafish locomotor activity, increased their HPA axis activity, and led to significant changes in the secretion of dopamine and serotonin. These findings provide us with a new understanding of the effects of ethanol on the HPA axis.

scholarly journals Ethanol affects behavior and HPA axis activity during development in zebrafish larvae

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wenxiao Du ◽  
Xiaoli Chen ◽  
Min Shi ◽  
Fuhua Bian ◽  
Zhenjun Zhao
Keyword(s):  
Hpa Axis ◽  
Ethanol Exposure ◽  
Enzyme Linked Immunosorbent Assay ◽  
Zebrafish Larvae ◽  
The Social ◽  
Excessive Intake ◽  
Key Genes ◽  
External Stimuli ◽  
Hpa Axis Activity

AbstractRecent studies have shown that long-term alcohol intake from food can lead to numerous mental disorders in humans, and the social and health effects of excessive intake of alcohol currently represent serious problems for governments and families worldwide. However, to date, it has not been determined how alcohol affects the hypothalamic–pituitary–adrenal (HPA) axis. The zebrafish offers a good model for studying the toxicology of food-grade ethanol. In the present study, using zebrafish larvae exposed to 1% ethanol, we performed zebrafish behavioral analysis. Samples were collected for enzyme-linked immunosorbent assay (ELISA) and quantitative real time-polymerase chain reaction (qRT-PCR) experiments, and statistical analysis was performed. We found that ethanol decreased the locomotor activity of zebrafish larvae, which showed a more intense reaction to external stimuli. Ethanol also increased the level of HPA axis hormones in zebrafish larvae, influenced the level of neurotransmitters, and altered the expression of key genes in neurotransmitter metabolism. Ethanol exposure affects zebrafish behavior, increases the level of HPA axis hormones in zebrafish larvae, affects the level of neurotransmitters, and affects the expression of key genes in dopamine and serotonin metabolism. These findings may help to elucidate the effects of ethanol on HPA axis activity.

scholarly journals Long-term caffeine intake exaggerates PTSD-like symptoms independent of HPA axis activity

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S259
Author(s):  
Santosh Kumar Prajapati ◽  
Sairam Krishnamurthy
Keyword(s):  
Hpa Axis ◽  
Caffeine Intake ◽  
Hpa Axis Activity

scholarly journals Characterization of Blueberry shock virus, an Emerging Ilarvirus in Cranberry

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Sara Thomas-Sharma ◽  
Lindsay Wells-Hansen ◽  
Rae Page ◽  
Victoria Kartanos ◽  
Erika Saalau-Rojas ◽  
...  
Keyword(s):  
Virus Transmission ◽  
Systemic Infection ◽  
Seed Transmission ◽  
Economic Consequences ◽  
Enzyme Linked Immunosorbent Assay ◽  
Polymerase Chain ◽  
Marketable Fruit ◽  
Mixed Virus Infection

Blueberry shock virus (BlShV), an Ilarvirus sp. reported only on blueberry, was associated with scarring, disfigurement, and premature reddening of cranberry fruit. BlShV was detected by triple-antibody sandwich enzyme-linked immunosorbent assay and reverse-transcription polymerase chain reaction, and isometric virions of 25 to 28 nm were observed in cranberry sap. The virus was systemic, although unevenly distributed in plants. The coat protein of BlShV from cranberry shared 90% identity compared with BlShV accessions from blueberry on GenBank. Phylogenetic analysis of isolates of BlShV from cranberry collected from Wisconsin and Massachusetts did not indicate grouping by state. BlShV was detected in cranberry pollen, and seed transmission of up to 91% was observed. Artificial inoculation of cranberry flowers by pollination did not cause virus transmission. In some Nicotiana spp., rub inoculation of leaves with homogenized BlShV-positive cranberry flowers resulted in systemic infection. Cranberry plants recovered from symptoms the year after berry scarring occurred but continued to test positive for BlShV. The virus caused significant reduction in the average number of marketable fruit and average berry weight in symptomatic cranberry plants but recovered plants yielded comparably with healthy plants. Although recovery may limit the immediate economic consequences of BlShV, long-term implications of single- or mixed-virus infection in cranberry is unknown.

scholarly journals Antenatal Corticosteroids: A Risk Factor for the Development of Chronic Disease

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Elizabeth Asztalos
Keyword(s):  
Preterm Birth ◽  
Hpa Axis ◽  
Animal Studies ◽  
Fetal Lung ◽  
Later Life ◽  
Long Term Effects ◽  
Antenatal Corticosteroids ◽  
Fetal Maturity ◽  
Hpa Axis Activity

Preterm birth remains a major health issue worldwide. Since the 1990s, women at risk for preterm birth received a single course of exogenous antenatal corticosteroids (ACSs) to facilitate fetal lung maturity. More recently, repeated or multiple courses of ACS have been supported to provide continued fetal maturity support for women with continued risk of preterm birth. However, exogenous ACS reduces birth weight which, in turn, is associated with adverse adult outcomes such as coronary heart disease, stroke, hypertension, and type 2 diabetes. The long-term effects of ACS exposure on HPA axis activity and neurological function are well documented in animal studies, and it appears that ACS, regardless of dose exposure, is capable of affecting fetal HPA axis development causing permanent changes in the HPA axis that persists through life and is manifested by chronic illness and behavioral changes. The challenge in human studies is to demonstrate whether an intervention such as ACS administration in pregnancy contributes to developmental programming and how this is manifested in later life.

scholarly journals Hypothalamic neurochemical changes in long-term recovered bilateral subdiaphragmatic vagotomized rats

2021 ◽  
Author(s):  
Anna Teresa Kobrzycka ◽  
Adrian Mateusz Stankiewicz ◽  
Joanna Goscik ◽  
Monika Gora ◽  
Beata Burzynska ◽  
...  
Keyword(s):  
Amino Acids ◽  
Hpa Axis ◽  
Alternative Pathway ◽  
Genome Wide ◽  
Inflammatory Processes ◽  
Prolonged Recovery ◽  
Central Nervous Systems ◽  
Neurochemical Changes ◽  
Hpa Axis Activity

Abstract BackgroundVagus nerve is one of the crucial routes in communication between immune and central nervous systems. Impaired vagal nerve function may intensify peripheral inflammatory processes. This effect subsides along with prolonged recovery after permanent nerve injury. One of the results of such compensation is a normalized plasma concentration of stress hormone corticosterone – a marker of hypothalamic-pituitary-adrenal (HPA) axis activity. In the present work we strive to explain this corticosterone normalization by studying the mechanisms responsible for compensation-related neurochemical alterations in the hypothalamus. Methods Using microarrays and HPLC we measured genome-wide gene expression and major amino acid neurotransmitters content in hypothalamus of bilaterally vagotomized rats, one month after surgery.ResultsOur results show that, in the long-term, vagotomy affects hypothalamic amino acids concentration but not mRNA expression of tested genes.ConclusionsWe propose an alternative pathway of immune to CNS communication after vagotomy, leading to activation of HPA axis, by influencing central amino acids and subsequent monoaminergic neurotransmission.

scholarly journals Postnatal Glucocorticoid Excess Due to Pituitary Glucocorticoid Receptor Deficiency: Differential Short- and Long-Term Consequences

Endocrinology ◽  
2009 ◽  
Vol 150 (6) ◽  
pp. 2709-2716 ◽  
Author(s):  
Mathias V. Schmidt ◽  
Vera Sterlemann ◽  
Klaus Wagner ◽  
Bertram Niederleitner ◽  
Karin Ganea ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Hpa Axis ◽  
Negative Feedback ◽  
Molecular Mechanisms ◽  
Forced Swim Test ◽  
Conditional Knockout ◽  
Negative Feedback Regulation ◽  
Long Term Consequences ◽  
Hpa Axis Activity

A tight regulation of hypothalamic-pituitary-adrenal (HPA) axis activity is essential for successful adaptation to stressful stimuli. Disruption of normal HPA axis development is a main risk factor for diseases such as posttraumatic stress disorder or depression, but the molecular mechanisms that lead to these long-term consequences are poorly understood. Here, we test the hypothesis that the pituitary glucocorticoid receptor (GR) is involved in regulating HPA axis function in neonatal and adult animals. Furthermore, we investigate whether postnatal hypercortisolism induced by pituitary GR deficiency is a main factor contributing to the persistent effects of early-life stress. Conditional knockout mice with a deletion of the GR at the pituitary (GRPOMCCre) show excessive basal corticosterone levels during postnatal development, but not in adulthood. The hypercortisolemic state of neonatal GRPOMCCre mice is accompanied by central gene expression changes of CRH and vasopressin in the paraventricular nucleus, but these alterations normalize at later ages. In adult mice, pituitary GR deficiency results in impaired glucocorticoid negative feedback. Furthermore, adult GRPOMCCre mice display a more active coping strategy in the forced swim test, with no alterations in anxiety like behavior or cognitive functions. Postnatal GR antagonist treatment is able to prevent the long-term behavioral effects in GRPOMCCre mice. In conclusion, we show that pituitary GRs are centrally involved in regulating HPA axis activity in neonates and mediate negative feedback regulation in adult animals. Postnatal glucocorticoid excess results in an altered stress-coping behavior in adult animals, with no effects on anxiety like behavior or cognition.

No Association of Antenatal Synthetic Glucocorticoid Exposure and Hair Steroid Levels in Children and Adolescents

2019 ◽  
Vol 105 (3) ◽  
pp. e575-e582
Author(s):  
Liesa Ilg ◽  
Clemens Kirschbaum ◽  
Shu-Chen Li ◽  
Pauline Wimberger ◽  
Katharina Nitzsche ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Stress Reactivity ◽  
Cross Sectional Study ◽  
Hair Cortisol ◽  
Control Groups ◽  
Dynamic Changes ◽  
Cross Sectional ◽  
Hpa Axis Activity ◽  
Synthetic Glucocorticoid

Abstract Context Antenatal synthetic glucocorticoid (sGC) treatment constitutes a potent programming factor of the hypothalamic–pituitary–adrenal (HPA) axis. Previous findings from our group revealed long-term changes in cortisol stress reactivity following antenatal sGC therapy. However, the few prior studies exclusively relied on spot measurements of phasic HPA axis activity, which may not adequately capture cortisol output over prolonged periods of time. Objective To address this gap, the current study utilized hair steroid concentrations, a valid marker of integrated long-term HPA-axis activity, to investigate endocrine changes in individuals treated with antenatal sGC. Design, Setting, and Participants This cross-sectional study comprised 76 term-born children (7–12 years) and 58 adolescents (14–18 years). Cumulated hormonal secretion in scalp hair over a 3-month period was determined for different biomarkers of tonic HPA axis activity by liquid chromatography coupled with tandem mass spectrometry. Hair steroid levels were compared between participants with antenatal sGC therapy (dexamethasone or betamethasone) and different control groups. Results Findings from this study provide no evidence for a significant effect of antenatal sGCs on long-term hair steroid concentrations. Participants treated with antenatal sGC exhibited comparable levels of hair cortisol, cortisone, dehydroepiandrosterone, and cortisol/dehydroepiandrosterone ratios compared to those of mothers who had been admitted to hospital for pregnancy complications but had never received sGC therapy and controls from physiological pregnancies. Conclusion In conjunction with data from previous studies, it is thus tempting to speculate that sGC may affect the capacity of dynamic changes and flexible adaption of an individual’s HPA axis rather than changes in tonic steroid output.

scholarly journals Attenuation of cortisol across development for victims of sexual abuse

2010 ◽  
Vol 22 (1) ◽  
pp. 165-175 ◽  
Author(s):  
Penelope K. Trickett ◽  
Jennie G. Noll ◽  
Elizabeth J. Susman ◽  
Chad E. Shenk ◽  
Frank W. Putnam
Keyword(s):  
Sexual Abuse ◽  
Childhood Abuse ◽  
Hpa Axis ◽  
Hierarchical Linear Modeling ◽  
Linear Trend ◽  
Early Adulthood ◽  
Linear Modeling ◽  
Morning Cortisol ◽  
Hpa Axis Activity

AbstractInconsistencies exist in literature examining hypothalamic–pituitary–adrenal (HPA) axis activity in children and adults who have experienced childhood abuse. Hence, the extent and manner to which childhood abuse may disrupt HPA axis development is largely unknown. To address these inconsistencies, the developmental course of nonstress cortisol in a long-term longitudinal study was assessed at six time points from childhood through adolescence and into young adulthood to determine whether childhood abuse results in disrupted cortisol activity. Nonstress, morning cortisol was measured in 84 females with confirmed familial sexual abuse and 89 nonabused, comparison females. Although dynamically controlling for co-occurring depression and anxiety, hierarchical linear modeling (HLM) showed that relative to comparison females, the linear trend for abused females was significantly less steep when cortisol was examined across development from age 6 to age 30,t(1, 180) = −2.55,p< .01, indicating attenuation in cortisol activity starting in adolescence with significantly lower levels of cortisol by early adulthood,F(1, 162) = 4.78,p< .01. As a more direct test of the attenuation hypothesis, supplemental HLM analyses of data arrayed by time since the disclosure of abuse indicated that cortisol activity was initially significantly higher,t(1, 425) = 2.18,p< .05, and slopes were significantly less steept(1, 205) = −2.66,p <.01, for abused females. These findings demonstrate how the experience of childhood abuse might disrupt the neurobiology of stress, providing some support for the attenuation hypothesis that victims of abuse may experience cortisol hyposecretion subsequent to a period of heightened secretion.

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