scholarly journals Heterologous Expression of ZmNF-YA12 Confers Tolerance to Drought and Salt Stress in Arabidopsis

2021 ◽  
Author(s):  
Tongtong Zhang ◽  
Dengyu Zheng ◽  
Chun Zhang ◽  
Zhongyi Wu ◽  
Rong Yu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Heterologous Expression ◽  
Stress Tolerance ◽  
Nacl Stress ◽  
Drought And Salt Stress ◽  
Potential Applications ◽  
Transgenic Lines ◽  
Peg Treatment ◽  
Mda Content ◽  
Drought And Salt Stresses

Abstract Drought and salinity are serious environmental factors limiting the growth and productivity of plants worldwide. Therefore, it is necessary to develop ways to improve drought and salinity stress tolerance in plants. In this study, a drought-responsive nuclear factor Y subunit A gene, ZmNF-YA12, was cloned from maize. qPCR revealed ZmNF-YA12 transcript in all vegetative and reproductive tissues, with higher levels in young roots. Expression analyses of maize revealed that ZmNF-YA12 was induced by abscisic acid (ABA), jasmonic acid (JA), and abiotic stresses, including dehydration, high salinity, cold, and polyethylene glycol (PEG) treatment. The heterologous expression of ZmNF-YA12 in Arabidopsis plants resulted in increased root length and better plant growth than in wild-type (WT) plants under conditions of mannitol, salt, and JA stress on 1/2 MS medium. Transgenic Arabidopsis showed improved tolerance to drought and salt stresses in soil, and higher proline content and lower malondialdehyde (MDA) content than WT controls. The transgenic plants also maintained higher peroxidase (POD) activities than WT plants under conditions of NaCl stress. A yeast two-hybrid experiment demonstrated that ZmNF-YA12 interacted with ZmNF-YC1 and ZmNF-YC15. Moreover, the transcript levels of stress-responsive genes (RD29A, RD29B, RAB18, and RD22) were markedly increased in transgenic lines under conditions of drought and salt stress. These observations suggested that the ZmNF-YA12 gene confers drought and salt stress tolerance, and has potential applications in molecular breeding with maintenance of production under conditions of stress.

Effects of Drought and Salt Stress on Activities of Antioxidant Protective Enzymes and Expression of Stress Genes in Alfalfa (Medicago sativa L.) Seedlings

2021 ◽  
Vol 15 (4) ◽  
pp. 553-558
Author(s):  
Zhifeng Chen ◽  
Haifeng Guo ◽  
Changling Sui ◽  
Zhixi Gao ◽  
Tianhong Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Resistance Genes ◽  
High Stress ◽  
Nacl Stress ◽  
Salt Resistance ◽  
Good Survival ◽  
Stress Genes ◽  
Drought And Salt Stress ◽  
Peg Treatment ◽  
Effects Of Drought

Drought and salt are main environmental factors that affect the growth, development, productivity and distribution of plants of plants. Alfalfa has a strong ability of early and salt resistance. In this work, the varieties Xinjiang Daye was used as material, the effects of drought (simulated with PEG, polyethylene glycol-6000) and salt stress (with NaCl solution) on the antioxidant capacity of alfalfa seedlings and stress resistance genes was studied, to select alfalfa varieties with strong resistance and study its functional principle of resistance related genes. The results showed that with the increase of drought stress, the contents of H2O2, O2−, MDA increased by 323, 247 and 235 (15% PEG treatment). The activities of SOD, CAT and APX increased by 18.01, 15.56 and 587% (15% PEG treatment), respectively. The expression of drought resistance genes increased significantly. With the increase in NaCl stress, the activities of SOD, cat, pod and APX increased by 132.14, 315.60, 102.78 and 27.61%, respectively. The expression of two genes related to salt stress increased significantly. In conclusion, alfalfa leaves have good survival ability under high stress, and the activities of antioxidant enzymes and the expression of related genes have adaptive changes under drought and salt stress.

scholarly journals Expression Analyses of Soybean VOZ Transcription Factors and the Role of GmVOZ1G in Drought and Salt Stress Tolerance

2020 ◽  
Vol 21 (6) ◽  
pp. 2177 ◽  
Author(s):  
Bo Li ◽  
Jia-Cheng Zheng ◽  
Ting-Ting Wang ◽  
Dong-Hong Min ◽  
Wen-Liang Wei ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Hairy Roots ◽  
Yeast Cells ◽  
Salt Stress Tolerance ◽  
Drought And Salt Stress

Vascular plant one-zinc-finger (VOZ) transcription factor, a plant specific one-zinc-finger-type transcriptional activator, is involved in regulating numerous biological processes such as floral induction and development, defense against pathogens, and response to multiple types of abiotic stress. Six VOZ transcription factor-encoding genes (GmVOZs) have been reported to exist in the soybean (Glycine max) genome. In spite of this, little information is currently available regarding GmVOZs. In this study, GmVOZs were cloned and characterized. GmVOZ genes encode proteins possessing transcriptional activation activity in yeast cells. GmVOZ1E, GmVOZ2B, and GmVOZ2D gene products were widely dispersed in the cytosol, while GmVOZ1G was primarily located in the nucleus. GmVOZs displayed a differential expression profile under dehydration, salt, and salicylic acid (SA) stress conditions. Among them, GmVOZ1G showed a significantly induced expression in response to all stress treatments. Overexpression of GmVOZ1G in soybean hairy roots resulted in a greater tolerance to drought and salt stress. In contrast, RNA interference (RNAi) soybean hairy roots suppressing GmVOZ1G were more sensitive to both of these stresses. Under drought treatment, soybean composite plants with an overexpression of hairy roots had higher relative water content (RWC). In response to drought and salt stress, lower malondialdehyde (MDA) accumulation and higher peroxidase (POD) and superoxide dismutase (SOD) activities were observed in soybean composite seedlings with an overexpression of hairy roots. The opposite results for each physiological parameter were obtained in RNAi lines. In conclusion, GmVOZ1G positively regulates drought and salt stress tolerance in soybean hairy roots. Our results will be valuable for the functional characterization of soybean VOZ transcription factors under abiotic stress.

scholarly journals Silencing of The Slzf-31 Gene Decreases The Salt Stress Tolerance And Drought Tolerance Of Tomato

2021 ◽  
Author(s):  
Tong Pei ◽  
Yufang Bao ◽  
Tairu Wu ◽  
Ziyu Wang ◽  
Yue Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Drought Stress ◽  
Virus Induced Gene Silencing ◽  
Chlorophyll Fluorescence Parameters ◽  
Tomato Plants ◽  
Drought And Salt Stress ◽  
Stem Bending ◽  
Physiological Indexes ◽  
Mda Content ◽  
Aba Content

Abstract The SlZF-31 gene is a member of the tomato C2H2 transcription factor family. Previous studies have shown that SlZF-31 gene expression is upregulated under drought stress and salt stress, but the specific function of this gene in tomato plants in response to these two kinds of stress is still unclear. To further explore the function of the SlZF-31 gene in tomato under drought stress and salt stress, we employed the virus-induced gene silencing (VIGS) method to reduce the expression of the SlZF-31 gene in tomato. The results showed that TRV2-SlZF-31 plants had higher levels of wilt and stem bending than CK and CK-TRV2 plants under drought and salt stress. The ABA content of TRV2-SlZF-31 plants were lower than those of CK and CK-TRV2 plants. The analysis of physiological indexes showed that the SOD and POD activity and the PRO content of TRV2-SlZF-31 plants were lower than those of CK and CK-TRV2 plants, while the MDA content of TRV2-SLlZF-31 plants was higher than those of CK and CK-TRV2 plants. The accumulation of H2O2 and O2- in TRV2-SlZF-31 plants was greater than those in CK and CK-TRV2 plants. The values of the chlorophyll fluorescence parameters (ΦII and qL) of TRV2-SlZF-31 plants were significantly lower than those of CK and CK-TRV2 plants. These results showed that the silencing of the SlZF-31 gene reduces the drought resistance and salt tolerance of tomato.

scholarly journals De-novo transcriptome analysis unveils differentially expressed genes regulating drought and salt stress response in Panicum sumatrense

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Rasmita Rani Das ◽  
Seema Pradhan ◽  
Ajay Parida
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Differentially Expressed Genes ◽  
Salinity Stress ◽  
Abiotic Stress Tolerance ◽  
Differentially Expressed ◽  
Little Millet ◽  
Drought And Salt Stress

AbstractScreening the transcriptome of drought tolerant variety of little millet (Panicum sumatrense), a marginally cultivated, nutritionally rich, susbsistent crop, can identify genes responsible for its hardiness and enable identification of new sources of genetic variation which can be used for crop improvement. RNA-Seq generated ~ 230 million reads from control and treated tissues, which were assembled into 86,614 unigenes. In silico differential gene expression analysis created an overview of patterns of gene expression during exposure to drought and salt stress. Separate gene expression profiles for leaf and root tissue revealed the differences in regulatory mechanisms operating in these tissues during exposure to abiotic stress. Several transcription factors were identified and studied for differential expression. 61 differentially expressed genes were found to be common to both tissues under drought and salinity stress and were further validated using qRT-PCR. Transcriptome of P. sumatrense was also used to mine for genic SSR markers relevant to abiotic stress tolerance. This study is first report on a detailed analysis of molecular mechanisms of drought and salinity stress tolerance in a little millet variety. Resources generated in this study can be used as potential candidates for further characterization and to improve abiotic stress tolerance in food crops.

scholarly journals Knockdown of Cytochrome P450 Genes Gh_D07G1197 and Gh_A13G2057 on Chromosomes D07 and A13 Reveals Their Putative Role in Enhancing Drought and Salt Stress Tolerance in Gossypium hirsutum

Genes ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 226 ◽  
Author(s):  
Richard Odongo Magwanga ◽  
Pu Lu ◽  
Joy Nyangasi Kirungu ◽  
Qi Dong ◽  
Xiaoyan Cai ◽  
...  
Keyword(s):  
Salt Stress ◽  
Gossypium Hirsutum ◽  
Stress Tolerance ◽  
Common Property ◽  
Stress Conditions ◽  
Tetraploid Cotton ◽  
Salt Stress Tolerance ◽  
Negative Effects ◽  
Drought And Salt Stress ◽  
Stress Responsive Genes

We identified 672, 374, and 379 CYPs proteins encoded by the CYPs genes in Gossypium hirsutum, Gossypium raimondii, and Gossypium arboreum, respectively. The genes were found to be distributed in all 26 chromosomes of the tetraploid cotton, with chrA05, chrA12, and their homeolog chromosomes harboring the highest number of genes. The physiochemical properties of the proteins encoded by the CYP450 genes varied in terms of their protein lengths, molecular weight, isoelectric points (pI), and even grand hydropathy values (GRAVY). However, over 99% of the cotton proteins had GRAVY values below 0, which indicated that the majority of the proteins encoded by the CYP450 genes were hydrophilic in nature, a common property of proteins encoded by stress-responsive genes. Moreover, through the RNA interference (RNAi) technique, the expression levels of Gh_D07G1197 and Gh_A13G2057 were suppressed, and the silenced plants showed a higher concentration of hydrogen peroxide (H2O2) with a significant reduction in the concentration levels of glutathione (GSH), ascorbate peroxidase (APX), and proline compared to the wild types under drought and salt stress conditions. Furthermore, the stress-responsive genes 1-Pyrroline–5-Carboxylate Synthetase (GhP5CS), superoxide dismutase (GhSOD), and myeloblastosis (GhMYB) were downregulated in VIGS plants, but showed upregulation in the leaf tissues of the wild types under drought and salt stress conditions. In addition, CYP450-silenced cotton plants exhibited a high level of oxidative injury due to high levels of oxidant enzymes, in addition to negative effects on CMS, ELWL, RLWC, and chlorophyll content The results provide the basic foundation for future exploration of the proteins encoded by the CYP450 genes in order to understand the physiological and biochemical mechanisms in enhancing drought and salt stress tolerance in plants.

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