LED Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer Optimizes Wound Repair in Rats

Abstract This study aimed to evaluate the effect of LED photobiomodulation therapy associated or not with heterologous fibrin biopolymer to repair skin wounds in rats. Full-thickness skin wounds were induced on the back of 84 Wistar rats and they were randomly divided into 4 groups: control group (CG), LED group (LED), heterologous fibrin biopolymer group (HFB), and LED + heterologous fibrin biopolymer group (LED + HFB). The animals were euthanized at 7, 14, and 21 days after surgery. In the macroscopic findings, it was observed in the control group, mild local inflammation, evidenced between the 5th and 6th day after the injury, not manifested in the other groups. The analysis of the wound reduction index shown that the treated groups presented greater wound reduction in the three periods evaluated. The results of the degree of inflammation showed that the LED and LED + HFB groups showed a predominance of chronic inflammation, while the control group showed a predominance of diffuse acute inflammation. The quantity of type I collagen it was bigger after 7 days in the LED and LED + HFB groups, and at 14 and 21 days this type of collagen appears more intensely in the group that used only HFB. The traction force at 7 days of the experiment, the treated groups showed better performance, while at 14 and 21 days the group that used only the biopolymer obtained greater strength in the suture region. Results have indicated that treatments using HFB and LED, either isolated or associated, stimulated the wound repair process in rats. HFB promote collagen maturation.

Download Full-text

Ozonized solutions favor the repair of experimentally induced skin wounds in rats

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-6578 ◽

2020 ◽

Vol 40 (11) ◽

pp. 914-921

Author(s):

Rafael C. Sanguanini ◽

Mariana F. Bento ◽

Evelyn de Oliveira ◽

Emmanuel Arnhold ◽

Mariana B.R. Faleiro ◽

...

Keyword(s):

Sodium Chloride ◽

Tissue Repair ◽

Wound Repair ◽

Type I Collagen ◽

Type I ◽

Skin Wounds ◽

The Third ◽

Picrosirius Red ◽

Morphometric Evaluation ◽

Experimentally Induced

ABSTRACT: This study aimed to evaluate and compare the effects of ozonized solutions on tissue wound repair in rats. Treatments consisted of ozonized water (GA), 0.9% sodium chloride (GCL), ozonized oil (GO), and 0.2% allantoin cream (GAL). The morphometric evaluation showed that wounds of the GA group presented a higher degree of retraction (p<0.05) at three and eight days of treatment (37.96 and 84.81%, respectively). Picrosirius red staining showed that groups GA and GO presented higher deposition (p<0.05) of type I collagen at 15 and 22 days of treatment, respectively. The neovascularization was higher in wounds of group GO on days 3, 8, and 15 (p<0.05), with higher VEGF immunostaining. (p<0.05). Thus, ozonized water enhances wound retraction and assists in the maturation and remodeling phase, while ozonized oil promotes higher neovascularization during tissue repair and higher deposition of type I collagen from the third week of treatment.

Download Full-text

(deleted)

Bulletin of Russian State Medical University ◽

10.24075/10.24075/brsmu.2021.003 ◽

2021 ◽

Author(s):

MV Osikov ◽

EV Davydova ◽

KS Abramov

Keyword(s):

Bone Healing ◽

Standard Therapy ◽

Type I Collagen ◽

Femoral Shaft ◽

Control Group ◽

Blood Levels ◽

Type I ◽

Mineral Density ◽

Fracture Consolidation ◽

To Receive

Efferent physical therapy holds promise as an adjunct to the combination treatment of femoral fractures in young, working-age individuals. The aim of the study was to investigate the dynamics of bone turnover markers at different stages of femoral fracture consolidation in patients undergoing ozone therapy. The study enrolled 20 men (group 2, 47.8 ± 3.5 years) with a femoral shaft fracture (AO/ASIF 32А, 32В). The control group (group 1, 46.8 ± 3.7 years) comprised 10 healthy males. Subgroup 2a (n = 10) was assigned to receive standard therapy; subgroup 2b (n = 10) was assigned to receive standard therapy complemented by minor autohemotherapy (MAHT) at 20 mg/L ozone concentrations. On days 7, 30 and 90, fracture consolidation was assessed on the RUST scale and blood levels of С-terminal telopeptides of type I collagen (bCTx, pg/ml) and procollagen type I carboxy-terminal propeptide (PICP, ng/ml) were measured. On day 7, the total RUST score in subgroups 2a and 2b was 4 points; on day 30, it was 6.5 and 8.7 points, respectively, and on day 90, it reached 10 and 11.5 points, respectively. Bone mineral density was as high as 90% in the MAHT subgroup vs. 78% in subgroup 2а, indicating faster bone healing. On day 30, bCTx levels in subgroup 2b were higher than in subgroup 2a (2289.4 [2145.3; 2365.4] vs. 1894.6 [1745.3; 2098.2], respectively. On day 7, PICP was significantly elevated in subgroup 2b in comparison with subgroup 2a; its levels peaked on days 30 and 90 (day 30: 268.3 [231.2; 286.3] vs. 183.2 [174.6; 195.6]; day 90: 584.6 [512.3; 589.3] vs. 351.2 [312.3; 369.4]. Thus, MAHT produces a positive effect on the quality and intensity of bone healing in men with isolated closed femoral shaft fractures.

Download Full-text

Electroacupuncture Ameliorates Subchondral Bone Deterioration and Inhibits Cartilage Degeneration in Ovariectomised Rats

Acupuncture in Medicine ◽

10.1136/acupmed-2016-011258 ◽

2018 ◽

Vol 36 (1) ◽

pp. 37-43 ◽

Cited By ~ 3

Author(s):

Jun Zhou ◽

Peirui Zhong ◽

Ying Liao ◽

Jing Liu ◽

Yuan Liao ◽

...

Keyword(s):

Subchondral Bone ◽

Type I Collagen ◽

Cartilage Degeneration ◽

Cross Linking ◽

Control Group ◽

Sham Operation ◽

Type I ◽

Trabecular Thickness ◽

Ovx Rats ◽

Time Point

Objectives To investigate the effects of electroacupuncture (EA) on subchondral bone mass and cartilage degeneration in an experimental animal model of osteoarthritis (OA) induced by ovariectomy (OVX). Methods Ninety 3-month-old female Sprague-Dawley rats were randomly divided into the following three groups (n = 30 each): sham operation without treatment (control group); OVX without treatment (OVX group);, and ovariectomy with EA treatment (EA group). Rats in the EA group received EA treatment from the day of OVX. Ten rats in each group were randomly killed at 4, 8 and 12 weeks after operation. Results EA reduced urine C-terminal cross-linking telopeptide of type I collagen from 4 weeks after OVX, reduced C-terminal cross-linking telopeptide of type II collagen and body weight from 8 weeks after OVX, and increased serum 17β-oestradiol from 4 weeks after OVX compared with the OVX group (all p<0.01). In the EA group, trabecular bone volume ratio, trabecular thickness and trabecular number increased, and trabecular separation were reduced at each time point compared with the OVX group (p<0.05, p<0.01, respectively). In the EA group, osteoprotegerin (OPG) expression was increased and receptor activator of nuclear factor kappa-B ligand (RANKL) expression was reduced at each time point compared with the OVX group (p<0.05, p<0.01, respectively). Mankin scores and mRNA expression of matrix metalloproteinase-13 (MMP-13) were lower in EA versus OVX groups at 12 weeks after OVX (both p<0.01). Conclusion The results suggest that EA inhibits subchondral bone loss by regulating RANK/RANKL/OPG signalling and protects articular cartilage by inhibiting MMP-13 in OVX rats.

Download Full-text

EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

ABCD Arquivos Brasileiros de Cirurgia Digestiva (São Paulo) ◽

10.1590/0102-672020190001e1498 ◽

2020 ◽

Vol 33 (1) ◽

Author(s):

Letícia Fuganti CAMPOS ◽

Eliane TAGLIARI ◽

Thais Andrade Costa CASAGRANDE ◽

Lúcia de NORONHA ◽

Antônio Carlos L. CAMPOS ◽

...

Keyword(s):

Diabetes Mellitus ◽

Wound Healing ◽

Type I Collagen ◽

Chronic Wounds ◽

Skin Wound ◽

Diabetic Rats ◽

Control Group ◽

Type I ◽

Collagen Deposition ◽

Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

Download Full-text

Targeted inhibition of TGF-β results in an initial improvement but long-term deficit in force production after contraction-induced skeletal muscle injury

Journal of Applied Physiology ◽

10.1152/japplphysiol.00374.2013 ◽

2013 ◽

Vol 115 (4) ◽

pp. 539-545 ◽

Cited By ~ 30

Author(s):

Jonathan P. Gumucio ◽

Michael D. Flood ◽

Anthony C. Phan ◽

Susan V. Brooks ◽

Christopher L. Mendias

Keyword(s):

Type I Collagen ◽

Transforming Growth Factor ◽

Force Production ◽

Transforming Growth Factor Β ◽

Control Group ◽

Type I ◽

Muscle Injuries ◽

Early Recovery ◽

Initial Improvement ◽

Fiber Atrophy

Transforming growth factor-β (TGF-β) is a proinflammatory cytokine that regulates the response of many tissues following injury. Previous studies in our lab have shown that treating muscles with TGF-β results in a dramatic accumulation of type I collagen, substantial fiber atrophy, and a marked decrease in force production. Because TGF-β promotes atrophy and fibrosis, our objective was to investigate whether the inhibition of TGF-β after injury would enhance the recovery of muscle following injury. We hypothesized that inhibiting TGF-β after contraction-induced injury would improve the functional recovery of muscles by preventing muscle fiber atrophy and weakness, and by limiting the accumulation of fibrotic scar tissue. To test this hypothesis, we induced an injury using a series of in situ lengthening contractions to extensor digitorum longus muscles of mice treated with either a bioneutralizing antibody against TGF-β or a sham antibody. Compared with controls, muscles from mice receiving TGF-β inhibitor showed a greater recovery in force 3 days and 7 days after injury but had a decrease in force compared with controls at the 21-day time point. The early enhancement in force in the TGF-β inhibitor group was associated with an initial improvement in tissue morphology, but, at 21 days, while the control group was fully recovered, the TGF-β inhibitor group displayed an irregular extracellular matrix and an increase in atrogin-1 gene expression. These results indicate that the inhibition of TGF-β promotes the early recovery of muscle function but is detrimental overall to full muscle recovery following moderate to severe muscle injuries.

Download Full-text

Cellular Compatibility of a New Tantalum-Niobium Scaffold Material

10.21203/rs.3.rs-39637/v1 ◽

2020 ◽

Author(s):

Jianan Ouyang ◽

Zhenhan Deng ◽

Kang Chen ◽

Jianyi Xiong ◽

Ying Li ◽

...

Keyword(s):

Cell Proliferation ◽

Type I Collagen ◽

Material Surface ◽

Control Group ◽

Type I ◽

Scaffold Material ◽

Rt Pcr ◽

Pcr Assay ◽

Porous Tantalum ◽

Significant Difference

Abstract [Objective] To determine the cellular compatibility of porous tantalum-niobium (Ta-Nb) material. [Method] Rabbit osteoblasts were co-cultured with porous Ta-Nb material. The cell proliferation was detected by CCK-8 method, and the cell adhesion was observed under scanning electron microscope (SEM). The expressions of type-I collagen and osteocalcin were detected by RT-PCR assay. [Results] CCK-8 detection indicated that the cell proliferation on the porous Ta-Nb material showed no difference from that of the control group (P>0.05). SEM revealed that a large amount of cells adhered onto the surface and in the pores of the material. The number of cells on the material surface increased obviously over time. RT-PCR assay showed that with the prolonging of the time of co-culture, the expression of type-I collagen was enhanced (P<0.05), while the osteocalcin expression exhibited no significant difference (P>0.05[Conclusion] Porous Ta-Nb scaffold material can be used to promote the adhesion, growth and differentiation of osteoblasts with satisfactory cellular compatibility.

Download Full-text

Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed to cadmium and/or alcohol

Toxicology and Industrial Health ◽

10.1177/0748233719831534 ◽

2019 ◽

Vol 35 (4) ◽

pp. 277-293 ◽

Cited By ~ 2

Author(s):

Iwona Markiewicz-Górka ◽

Piotr Kuropka ◽

Lidia Januszewska ◽

Aleksandra Jaremków ◽

Paweł Pawłowski ◽

...

Keyword(s):

Physical Training ◽

Type I Collagen ◽

Morphological Changes ◽

Female Rats ◽

Control Group ◽

Bone Resorption Marker ◽

Type I ◽

Mineral Contents ◽

Morphological Alterations ◽

Bone Parameters

The aim of the study was to investigate the effect of physical training on bone parameters of rats exposed to alcohol (Al) and/or cadmium (Cd). Young female rats were divided into one control group and six groups exposed to Cd and/or Al. Al (36% calories of diet) and Cd (20 mg Cd/kg feed) were administered with liquid diet. Half of the rats from the treated groups were subjected to treadmill training (20 m/min for 0.5 h, 4 days a week). The experiment was carried out for 5 months. Al decreased the concentration of calcium (Ca) and iron (Fe) in the femur, whereas Cd and Cd + Al intake reduced the contents of Ca, Fe and zinc. Al and/or Cd caused an increase in both C-terminal telopeptide of type I collagen (CTX1; bone resorption marker) and osteocalcin (OC; formation indicator) and enhanced the degree of porosity and flexural strength of the femur. Al partially prevented the loss of Fe from the bone caused by Cd, but intensified the inhibition of growth of body weight in comparison with separate exposure to Cd. In rats co-exposed to Cd + Al, the levels of CTX1 were greater compared with those treated with Al or Cd separately, and the density was less than that in rats exposed to Al separately. The training caused increases of magnesium and Ca contents, decreases in CTX1, as well as increases in OC and bone density, decreasing their porosity. The effect of training on the bone status, however, was limited (especially in rats co-exposed to Cd and Al) because of the increase in their mineralization, stimulated by exercises, was insufficient in relation to collagen production intensity. In conclusion, training had favourable effects on some bone parameters, but did not compensate for the negative effects of Al and/or Cd exposure on the poor mineralization and histopathological and morphological changes in the femur.

Download Full-text

Induction of interstitial collagenase in human keratinocytes during wound repair requires contact with native type I collagen

Matrix Biology ◽

10.1016/0945-053x(94)90091-4 ◽

1994 ◽

Vol 14 (5) ◽

pp. 379

Author(s):

W.C. Parks ◽

B.D. Sudbeck ◽

U.S. Saariahlo-Kere ◽

A.P. Pentland ◽

H.G. Welgus

Keyword(s):

Wound Repair ◽

Type I Collagen ◽

Human Keratinocytes ◽

Type I ◽

Interstitial Collagenase

Download Full-text

Evaluation of Indonesian and two types of Japanese honey on the acceleration of cutaneous wound healing: an experimental study in mice

Journal of Health Technology Assessment in Midwifery ◽

10.31101/jhtam.912 ◽

2019 ◽

Vol 2 (2) ◽

pp. 80-88

Author(s):

Haryanto Haryanto ◽

Kanae Mukai ◽

Nakajima Yukari ◽

Mayumi Okuwa ◽

Cau Kim Jiu ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Control Group ◽

Wound Healing Process ◽

Wound Area ◽

Hydrocolloid Dressing ◽

Thickness Skin ◽

Microscopic Evaluation ◽

Acacia Honey ◽

Macroscopic Findings

The purpose of this study is to evaluate and clarify the effect of Indonesian honey, two types of Japanese honey and hydrocolloid dressing on wound healing process. Four groups of male mice were treated to produce two circular full-thickness skin wounds on the dorsum. They were then randomly allocated to receive daily Indonesian honey, Japanese honey (Buckwheat and Acacia honey) or hydrocolloid dressing as a control for treatment application. Macroscopic findings were observed from day 0 to 14 after wounding. Microscopic evaluation was assessed using qualitative analysis. The ratios of wound areas for honey groups on day 3 and 7 were smaller than those of the control group. Wound areas of honey groups gradually decreased to almost the same wound area as the control group on day 14, while the wound area of the control group peaked on day 5 and rapidly decreased until day 14. Microscopic finding that Indonesian honey was different with Japanese honey especially Buckwheat honey.

Download Full-text

Tissue Response of TM-Joint to Arthritis Induction Methods in Rat

UI Proceedings on Health and Medicine ◽

10.7454/uiphm.v1i0.48 ◽

2017 ◽

Vol 1 ◽

Author(s):

Ding Han Wang ◽

Mu Chen Yang ◽

Wun Eng Hsu ◽

Ming Lun Hsu

Keyword(s):

Bone Growth ◽

Type I Collagen ◽

Tissue Response ◽

Control Group ◽

Type I ◽

Injection Group ◽

Group 4 ◽

Joint Disorder ◽

Group 3 ◽

Group 2

Objective: Pathogenesis of rheumatoid arthritis (RA) related temporomandibular joint disorder remains unclear. Some studies investigated the change of condyle after Complete Freund's Adjuvant (CFA) injection which is similar to osteoarthritis. However, there were few studies to demonstrate effect of TMJ in CIA which mimics RA. The aim of this study was to investigate the TM-joint response of CFA and CIA group in rat model.Methods: 24 Rats were divided into 4 groups. (1) Control group. (2) 50μl type I collagen injection group. (3) 50μl CFA injection group. (4) 25μl type I collagen + 25μl CFA injection group. Drug injection was performed on day 0 and day 14 and sacrificed on day 7, and day 35. After sacrificed, TMJ tissue was collected to do the H&E stain and investigated the inflammatory (IL-1β and MMP3) gene expression.Results: TMJ tissue of CFA group demonstrated adaptive bone growth. On the other hand, the subchondral bone of CIA group appeared degenerative process. The trend of IL-1β and MMP3 gene increased in CFA and CIA group compared to control group in synovial tissue and disc (p<0.05).Conclusion: Within limitation of this study, it’s concluded that the CIA method can be used in RA related TMD mechanism research.

Download Full-text