scholarly journals Histometric and Histological Analysis of Bone to Screw Contact Between Metallic and Absorbable 2.0 Fixation System: A Pilot Study in Dogs

2021 ◽  
Author(s):  
Felipe Perraro Sehn ◽  
Rogério Kato ◽  
Paulo de Oliveira ◽  
Alexandre Trivellato ◽  
Cássio Sverzut
Keyword(s):  
Connective Tissue ◽  
Histological Analysis ◽  
Tissue Formation ◽  
Male Adult ◽  
The Third ◽  
Control Side ◽  
System A ◽  
Experimental Side ◽  
Two Miniplates ◽  
Fixation System

Abstract ObjectiveTo evaluate the bone/screw interface of a titanium IF system and an absorbable IF system.Materials and methodsTwelve male adult dogs were subjected to osteotomy performed between the third and fourth mandibular premolars, been divided into two groups (control or experimental side). Two miniplates were applied to fix mandible fragments. Histometric values for bone-to-screw contact (BSC), bone-to-screw distance (BSD) and histological findings were evaluated at 2 and 18 weeks.ResultsDifference in BSC were detected from absorbable and metallic monocortical group on the experimental side at 2 weeks (p = 0.0041). Larger BSDs were detected at buccal bicortical absorbable experimental sides (p = 0.0041), with larger distances detected from the vestibular side to the lingual cortical side (p < 0.0001) and on the control side at 18 weeks (p = 0.0143), having metallic group significant BSD results on the experimental side at 2 weeks (p = 0.047). Connective tissue was noted on vestibular cortical side in monocortical and bicortical absorbable screw groups. ConclusionsAbsorbable bicortical screws with splint had histometric and histological behaviors similar to metallic bicortical group at lingual cortical side.Clinical RelevanceInion absorbable bicortical screws did not promote connective tissue formation at lingual cortical side.

The Effect of the Supra-alveolar Soft Tissue on the Relapse of Orthodontic Treatment

1983 ◽  
Vol 10 (1) ◽  
pp. 50-52 ◽  
Author(s):  
Erik Larsson ◽  
György Schmidt
Keyword(s):  
Soft Tissue ◽  
Connective Tissue ◽  
Orthodontic Treatment ◽  
Histological Analysis ◽  
Inflammatory Cells ◽  
Connective Tissues ◽  
Control Side ◽  
Definite Difference ◽  
The Stability

Orthodontic closure in cases of aplasia as also in patients with spaces of other genesis is often associated with problems of relapse. In this study ten patients were treated orthodontically in order to close unacceptable interdental spaces. Five patients had paired aplasia. The remaining five patients had interdental spaces of different genesis. Supra-crestal connective tissue fibres w re surgically removed in all patients but only on one side of the jaw in the children with paired aplasia. The non-operated side of these children served as a control side. The stability of the orthodontic treatment was acceptable. No definite difference between the operated and the control side was recorded. The histological analysis of the removed tissue did not reveal anything unexpected but a rich infiltration of inflammatory cells. It is suggested that inflammation might aid the reorganization of the connective tissues in this area.

scholarly journals Reaction of Subcutaneous Connective Tissue of Experimental Animals on Bone Marrow Mesenchymal Stromal Cell Coated Hydroxyapatite

2012 ◽  
Vol 12 (1) ◽  
pp. 45-50
Author(s):  
Aleksejs Zavorins ◽  
Mara Pilmane ◽  
Girts Salms ◽  
Andrejs Skagers ◽  
Inese Cakstina ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Connective Tissue ◽  
Apoptotic Cell ◽  
Subcutaneous Tissue ◽  
Donor Site ◽  
Operation Time ◽  
Tissue Quality ◽  
Experimental Animals ◽  
Control Side ◽  
Experimental Side

Summary Introduction. Hydroxyapatite (HAp) scaffolds have become an alternative to autologous bone grafts in orthopedic surgery, because it is possible to fill larger scale bone defects and of the decreased operation time and complication risks at the graft donor site, a promising method in bone regeneration is to load bone marrow - mesenchymal stromal cells (BM-MSCs) on to a HAp scaffold. Aim of the Study. The aim of our study is to compare subcutaneous tissue reaction of experimental animals to implantation of HAp and HAp that is coated with BM-MSCs. Materials and Methods. Autologous BM-MSCs were cultivated from crista iliaca dextra biopsy, 4 HAp ceramic implants were coated with BM-MSC and implanted in subcutaneous tissue on one side and non-coated HAp implants on the other side of the spine of 4 rabbits. Immunohistochemical staining of BM-MSC and connective tissue included TUNEL assay, NFkBp105, HSP 70k, Wnt1, TNF-α, VEGF, MMP-2 antibodies, microscoped at 400X magnification and analyzed semiquantitatively. Results. Routine staining with haemotoxilin and eosin demonstrated formation of granulation tissue around the HAp implant, which was more distinct on the control side. There was an increased number of NFκB p105 positive cells (fibroblasts, myocytes, endotheliocytes) on the experimental side (+++), in comparison to the control side (++). HSP70 and apoptosis index was decreased on the experimental side (++ and 60%), in comparison to the control side (+++ and 70%). Number of MMP2 positive structures was increased on the control side in the fibrous capsule (++), in comparison to the experimental side, where the result varied (0 - +) Conclusions. Cultured BM-MSC activity is partly limited due to the apoptosis, which is probably induced by environmental factors. BM-MSC coated HAp implant stimulates secretion of proliferation marker NFkBp105 in subcutaneous tissue, decreases cell stress, apoptosis and tissue degradation (decreased HSP 70, apoptotic cell count and MMP 2) to improve the local tissue quality and proving BM-MSC tissue protective effect

Histology of the vertebral artery–dural junction: relevance to posterolateral approaches to the skull base

2019 ◽  
pp. 1-6
Author(s):  
Robert C. Rennert ◽  
Martin P. Powers ◽  
Jeffrey A. Steinberg ◽  
Takanori Fukushima ◽  
John D. Day ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Vertebral Artery ◽  
Skull Base ◽  
Histological Analysis ◽  
Foramen Magnum ◽  
Entry Site ◽  
Microsurgical Technique ◽  
Vessel Injury ◽  
Far Lateral ◽  
Clear Delineation

OBJECTIVEThe far-lateral and extreme-lateral infrajugular transcondylar–transtubercular exposure (ELITE) and extreme-lateral transcondylar transodontoid (ELTO) approaches provide access to lesions of the foramen magnum, inferolateral to mid-clivus, and ventral pons and medulla. A subset of pathologies in this region require manipulation of the vertebral artery (VA)–dural interface. Although a cuff of dura is commonly left on the VA to avoid vessel injury during these approaches, there are varying descriptions of the degree of VA-dural separation that is safely achievable. In this paper the authors provide a detailed histological analysis of the VA-dural junction to guide microsurgical technique for posterolateral skull base approaches.METHODSAn ELITE approach was performed on 6 preserved adult cadaveric specimens. The VA-dural entry site was resected, processed for histological analysis, and qualitatively assessed by a neuropathologist.RESULTSHistological analysis demonstrated a clear delineation between the intima and media of the VA in all specimens. No clear plane was identified between the connective tissue of the dura and the connective tissue of the VA adventitia.CONCLUSIONSThe VA forms a contiguous plane with the connective tissue of the dura at its dural entry site. When performing posterolateral skull base approaches requiring manipulation of the VA-dural interface, maintenance of a dural cuff on the VA is critical to minimize the risk of vascular injury.

scholarly journals The action of demineralized bovine bone matrix on bone neoformation in rats submitted to experimental alcoholism

2013 ◽  
Vol 65 (3) ◽  
pp. 715-721 ◽  
Author(s):  
R.L. Buchaim ◽  
J.C. Andreo ◽  
A. C. Rodrigues ◽  
D.V. Buchaim ◽  
D.V. Dias ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Repair ◽  
Blood Clot ◽  
Bone Matrix ◽  
Male Rats ◽  
Bovine Bone ◽  
Filling Material ◽  
Tissue Formation ◽  
Bone Trabeculae ◽  
Bone Tissue Formation

The objective of this study was to evaluate whether demineralized bovine bone (Gen-ox®) alters bone neoformation in rats submitted to alcoholism. Forty male rats were separated into two groups of 20 rats and distributed as follows: Group E1, which received 25% ethanol and a surgical cavity filled only by a blood clot, and Group E2, which received 25% ethanol and a surgical cavity filled with Gen-ox®. The animals were euthanized at 10, 20, 40 and 60 days after surgery and necropsy was performed. The histomorphological and histometric analyses of the area of connective tissue and bone neoformation showed that the reorganization of the bone marrow and full repair of the surgical cavity in Group E1 occurred more quickly than in Group E2. It was also noted that in the final period the animals in Group E2 showed areas of connective tissue and thick bone trabeculae around the particles of the implant. It can be concluded that the use of Gen-ox® delayed the process of bone repair in alcoholic rats, although it can be used as filling material because it shows osteoconductive activity, as evidenced by bone tissue formation around the graft particles.

scholarly journals Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

2008 ◽  
Vol 19 (1) ◽  
pp. 40-45 ◽  
Author(s):  
Ana Teresa Sant'anna ◽  
Luis Carlos Spolidório ◽  
Lizeti Toledo Oliveira Ramalho
Keyword(s):  
Connective Tissue ◽  
Chronic Inflammation ◽  
Histological Analysis ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Connective Tissues ◽  
Plastic Tube ◽  
Tissue Samples ◽  
Subcutaneous Connective Tissue ◽  
Original Formula

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

scholarly journals Preparative-Capillary Gas Chromatographic Systems for Trace Analysis of Rum

1969 ◽  
Vol 60 (4) ◽  
pp. 559-584
Author(s):  
Heriberto Batiz ◽  
Elena Soltero
Keyword(s):  
Stainless Steel ◽  
Puerto Rican ◽  
Alcoholic Beverages ◽  
Complete Analysis ◽  
Volatile Fraction ◽  
Rapid Preparation ◽  
Porapak Q ◽  
The Third ◽  
System A ◽  
In Series

Three gas chromatographic systems a ppropriate for the reproducible rapid preparation and subsequent quantitative analysis of rum concentrates were developed and tested. The first and second systems employed preparative columns directly coupled to a capillary Golay column. Rum concentrates obtained in the preparative column were collected in a stainless steel trap of low dead space and simultaneously analyzed in the capillary system by flash heating of the trap. The third system used the same chromatographic equipment, with a 1/4-in o.d. trap to transfer an amount of concentrate to a 2-ml vial. These concentrates were subsequently analyzed with two Golay columns. In the first system, a 10-ft 20% Carbowax 20M packed preparative column coupled to a 150-ft Golay column through a 1/8-in o.d. trap gave excellent results in the analysis of the volatile fraction of rums. Ten congeners eluting before alcohol were detected in a commercial rum, six of which have not been identified. The second system, utilizing the 10-ft Carbowax 20M coupled to a 7-ft Porapak Q preparative column, was tested successfully with a 1/8-in o.d. trap. About 30 congeners were detected in the same commercial Puerto Rican rum. A 7-ft Porapak Q packed preparative column was investigated in the third system provided with a 1/4-in o.d. trap. About 40 congeners were detected in two commercial Puerto Rican rums utilizing a 50-ft SE-30 Golay column in series with a 150-ft Carbowax 20M Golay column. Results demonstrate the feasibility of obtaining a rapid and complete analysis of distilled alcoholic beverages.

Publishing and the Intellectual System

1975 ◽  
Vol 421 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Philip G. Altbach
Keyword(s):  
Mass Media ◽  
Third World ◽  
Book Industry ◽  
Intellectual Community ◽  
The Third ◽  
System A ◽  
Intellectual System ◽  
Industrialized Nations ◽  
The Third World ◽  
Relationship Of

Publishing is an integral part of the total intellectual system of any nation and of an international relationship of cultural and educational matters. Publishers are very much influenced by currents in their societies. Such factors as levels of literacy, habits of book purchasing, libraries, copyright regulations and bookstores have an impact on the nature of publishing and the book industry. The educational system, a particularly important consumer of books, is crucial to publishing. In industrialized nations, where levels of literacy are high, it is likely that publishing will be highly developed although it is under increasing attack from such forces as television and other mass media. In the Third World, the mass media are not highly developed, and books often have a particularly important role. This article argues that it is impossible to ignore the broader elements of the modern intellectual community in discussing publishing, and that books continue to play a key role in the development of that community and of the culture in general.

The Connective Tissue Sheath of the Locust Nervous System: A Histochemical Study

1959 ◽  
Vol s3-100 (51) ◽  
pp. 401-412
Author(s):  
DOREEN E. ASHHURST
Keyword(s):  
Nervous System ◽  
Connective Tissue ◽  
Collagen Type ◽  
Histochemical Study ◽  
Locusta Migratoria ◽  
System A ◽  
Large Numbers ◽  
Connective Tissue Sheath ◽  
Cellular Layer ◽  
Sheath Cells

The connective tissue sheath surrounding the nervous system of Locusta migratoria has been studied histochemically. It consists of an outer non-cellular layer, the neural lamella, and an inner layer of cells, the sheath-cells. The neural lamella has been identified as being composed of a collagen-type protein and neutral mucopolysaccharide on the evidence of its histochemical reactions and the identification of hydroxyproline by paper chromatography in a hydrolysate of the neural lamella. The sheath-cells possess large numbers of lipochondria composed of phospholipids and cerebrosides, and small spherical mitochondria. The cytoplasm also contains lipids (some of which may be cerebrosides), glycogen, and RNA.

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