scholarly journals Protective effects of phytomediated synthesized magnesium hydroxide nanomaterials using Monodora myristica against diabetes-associated hepatopathy, nephropathy and neuropathy in streptozotocin-exposed rat

2021 ◽  
Author(s):  
Olakunle Bamikole Afolabi ◽  
Omotade I Oloyede ◽  
Bukola T Aluko ◽  
Jonathan Johnson
Keyword(s):  
Diabetes Mellitus ◽  
Wistar Rats ◽  
Low Dose ◽  
Diabetic Rats ◽  
Enzymatic Antioxidants ◽  
Protective Effects ◽  
Control Groups ◽  
Male Wistar Rats ◽  
Monodora Myristica ◽  
Standard Techniques

Abstract There is recently a fast growing interest in green nanotechnology as a better approach for managing diabetes mellitus (DM) and its associated complications. This study therefore aimed at exploring the protective potentials of biogenic phytomediated magnesium-based nanoparticles using Monodora myristica seed (Mg(OH)2NP-Mm) against streptozotocin-induced diabetic hepatopathy, nephropathy and neuropathy in Wistar rats. Mg(OH)2NP-Mm was biosynthesized and characterized using standard techniques. Forty-eight (48) adult male Wistar rats weighing 150-200 g, were indiscriminately grouped into eight (8) groups of six (6) rats (n=6) each. Diabetes was induced with a low dose of streptozotocin (STZ) (55 mg/kg bw) and diabetic animals administered 50, 100, 150 and 200 mg/kg bw Mg(OH)2NP-Mm for 21 days, while control groups received glibenclamide (5 mg/kg bw) and Mg(OH)2-STD (150 mg/kg bw), respectively. In this study, treatment with Mg(OH)2NP-Mm caused a significant (p < 0.05) improvement in fasting blood sugar (FBG), serum hepatic biomarkers (AST, ALT and ALP activities), renal clearance markers (creatinine and urea), total protein (TP) and bilirubin. Enzymatic and non-enzymatic antioxidants, as well as histomorphological examinations indicated a significant (p < 0.05) restoration of the hepatic, renal and brain tissues. This finding therefore, hypothesizes attenuation of redox imbalance which probably could be the basis for the protective effect demonstrated by Mg(OH)2NP-Mm in the tissues examined from STZ-induced diabetic rats.

scholarly journals The Influence of Goat Milk and Soybean Milk Kefir On IL-6 and Crp Levels in Diabetic Rats

2015 ◽  
Vol 22 (3) ◽  
pp. 261-267 ◽  
Author(s):  
◽  
Agatha Swasti Ayuning Tyas ◽  
Scolastika Dita Kristian ◽  
◽  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Plasma Glucose ◽  
Wistar Rats ◽  
Goat Milk ◽  
Diabetic Rats ◽  
Significant Decline ◽  
Soybean Milk ◽  
Male Wistar Rats

Abstract Background and Aims: Hyperglycemia increases inflammation in type 2 diabetes mellitus (T2DM). Goat and soybean milks have been known to reduce inflammation. The aim of this study is to evaluate the effect of goat milk and soybean milk kefir on IL-6 and CRP levels in diabetic rats. Materials and Method: A total of 25 male Wistar rats were divided into the following groups: 1) normal rats; 2) diabetic rats; 3) diabetic rats with intake of goat milk kefir; 4) diabetic rats with intake of soybean milk kefir; and 5) diabetic rats with combination of both types of kefir. Before kefir administration for 4 weeks, plasma glucose was measured. After treatment, plasma glucose, CRP and IL-6 levels were assessed. Results: There was a significant decline of plasma glucose in diabetic rats with goat milk kefir (p=0.021), and in diabetic rats with the combination of both goat milk and soybean milk kefir (p<0.001). After treatment, CRP level in diabetic rats was significantly higher when compared with those that had goat milk kefir (p=0.043). All of diabetic rats with kefir had IL-6 values significantly lower than diabetic rats without it (p=0.021). Conclusion: Both goat milk and soybean milk kefir can reduce IL-6 level, but only goat milk kefir can reduce CRP in diabetic rats.

scholarly journals Study on Synergistic Effect of Bromocriptine and Sitagliptin in Streptozotocin-induced Diabetic Rats

2021 ◽  
Author(s):  
Govindaraj Nileshraj ◽  
Chandrasekaran Swithraa ◽  
Murugesan Sakthibalan ◽  
Maruti Shripati Sawadkar
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Lipid Profile ◽  
Blood Sugar ◽  
Wistar Rats ◽  
Low Dose ◽  
Diabetic Rats ◽  
Fasting Blood Sugar ◽  
Liver Parameters ◽  
Dose Combination

Introduction: Diabetes mellitus is a potentially morbid condition with high prevalence worldwide. Restoration of euglycemia in type 2 diabetes mellitus is difficult to achieve due to its multifactorial pathogenesis and frequently requires multiple anti-diabetic medications with different mechanism of action. This study is based on the possible synergistic effect of Sitagliptin and Bromocriptine in reducing blood sugar in diabetic animal models. Aim: To evaluate the effect of Sitagliptin and Bromocriptinemonotherapy and their combination in three different doses on blood sugar, Lipid profile, Liver and renal parameters in diabetic albino Wistar rats. Materials and Methods: An experimental animal study was conducted on 48 adult male albino wistar rats for a period of one month (August 2014). Streptozotocin induced (40 mg/kg IP) diabetic rats were divided into six groups of eight animals each weighing 150-200 gms. Group I- Vehicle (distilled water), Group II- Sitagliptin (100 mg/kg), Group III- Bromocriptine (10 mg/kg), Group IV- Sitagliptin (75 mg/kg) + Bromocriptine (7.5 mg/kg), Group V- Sitagliptin (100 mg/kg) + Bromocriptine (10 mg/kg), Group VI- Sitagliptin (125 mg/kg) + Bromocriptine (12.5 mg/kg). All drugs were given once daily (p.o) for four weeks. Body weight, fasting blood sugar, fasting lipid profile, liver parameters and renal parameters were estimated before and after administration of the drugs. Results were analyzed with SPSS software version 16.0 using one-way ANOVA followed by post-hoc Dunnett t test. P<0.05 was considered statistically significant. Results: The low dose combination therapy [Sitagliptin (75 mg/kg) + Bromocriptine (7.5 mg/kg)] showed a significant decrease in body weight (p<0.05), fasting blood sugar (p<0.001) compared with the vehicle group. It also showed a favorable lipid profile, renal and liver parameters compared with the vehicle, monotherapy and other combination regimens. Conclusion: The low dose combination therapy of Sitagliptin and Bromocriptine showed a better control over fasting blood sugar along with a beneficial effect on lipid profile, liver parameters and renal parameters than compared to other groups.

Hepatoprotective effect of seabuckthorn leaf extract in streptozotocin induced diabetes mellitus in Wistar rats

2018 ◽  
Author(s):  
P. Khajuria ◽  
P. Raghuwanshi ◽  
A. Rastogi ◽  
A. L. Koul ◽  
R. Zargar ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Hepatoprotective Effect ◽  
Male Wistar Rats ◽  
Streptozotocin Induced Diabetes

Study was conducted to evaluate the hepatoprotective effects of Seabuckthorn leaf extract (SLE) supplementation on serum enzymatic levels in streptozotocin (STZ) induced diabetes mellitus in Wistar rats. Thirty-two adult male Wistar rats were divided into four groups namely CON (negative control), SCO (Seabuckthorn control), DCO (Diabetic control), and DSL (Diabetic seabuckthorn treatment group). Diabetes mellitus was induced by single intra peritoneal injection of STZ @ 50 mg/kg body weight in DCO and DSL group of rats. SLE was administered orally @ 100mg/kg body weight for 40 days to SCO and DSL groups. CON served as the negative control. Blood samples were collected from experimental animals on zero, 20th, and 40th days of trial to study liver specific serum enzyme profile viz aspartate amino transaminase (AST), alanine amino transaminase (ALT), alkaline phosphatase (ALP) and acid phosphatase (ACP). Significantly (P less than 0.01) higher levels of all the enzymes studied were observed in experimentally induced diabetic rats in comparison to normal rats. However, in SLE treated diabetic rats (DSL group), significant (P less than 0.01) improvement was observed in all the above enzymes. It may be concluded that SLE exerts hepatoprotective effect in STZ induce Diabetes mellitus in Wistar rats.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Biochemical and histopathological effects of low dose vanadium in the healing of acetic acid-induced colitis in male wistar rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Tosan Peter Omayone ◽  
Samuel Babafemi Olaleye
Keyword(s):  
Acetic Acid ◽  
Untreated Control ◽  
Wistar Rats ◽  
Low Dose ◽  
Healing Process ◽  
Beneficial Effects ◽  
Male Wistar Rats ◽  
Haematological Changes ◽  
Therapeutic Properties ◽  
Histopathological Effects

Abstract Objectives Vanadium has been reported to possess relevant therapeutic properties such as anti-diabetic and anti-tumoral. This study aimed at determining the effects of vanadium on experimentally induced colitis in rats. Methods Forty-five male Wistar rats (103 ± 3.90 g, n=15) were used for this study and were divided into three groups. Group 1 (Untreated control) had nothing added to their drinking, while groups 2 and 3 received sodium metavanadate at a dose of 50 and 200 mg/L respectively in their drinking water for 10 weeks. Colitis was thereafter induced by intra colonic administration of 1.50 mL of 6% acetic acid. Animals were sacrificed on day 0 (pre-induction), three- and seven-days post induction. Blood samples were collected for haematological variables and the distal 8 cm of the colon was collected for macroscopic, histological and biochemical (malondialdehyde-MDA, superoxide dismutase-SOD, catalase-CAT, glutathione peroxidase- GPx and nitrite concentration- NO) assessment. Results Low dose vanadium proved beneficial in ameliorating acetic acid-induced colitis by improving both histopathological and haematological changes. Gross observation showed a faster healing rate in vanadium treated groups (50 and 200 mg/L) compared with untreated control at day 3 (40 and 26.20 vs. 2.50%) and day 7 (80 and 66.70 vs. 42%) respectively. Vanadium also appears to exert its beneficial effects on acetic acid-induced colitis via up regulation of antioxidant enzymes (SOD, CAT, GPx) and NO while decreasing the over production of MDA. Conclusions Vanadium at small concentration functions as an essential trace element and may be able to promote healing process during ulcerative colitis.

Attenuation of erythrocyte membrane oxidative stress bySesbania grandiflorain streptozotocin-induced diabetic rats

2015 ◽  
Vol 93 (4) ◽  
pp. 385-395 ◽  
Author(s):  
Chandrabose Sureka ◽  
Thiyagarajan Ramesh ◽  
Vavamohaideen Hazeena Begum
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Erythrocyte Membrane ◽  
Osmotic Fragility ◽  
Diabetic Rats ◽  
Glycosylated Haemoglobin ◽  
Albino Rats ◽  
Enzymatic Antioxidants ◽  
Protective Effects

The aim of the present study was to investigate the protective effects of Sesbania grandiflora flower (SGF) extract on erythrocyte membrane in Streptozotocin (STZ)-induced diabetic rats. Adult male albino rats of Wistar strain, weighing 190–220 g, were made diabetic by an intraperitonial administration of STZ (45 mg/kg). Normal and diabetic rats were treated with SGF, and diabetic rats were also treated with glibenclamide as drug control, for 45 days. In this study plasma insulin and haemoglobin levels were decreased and blood glucose, glycosylated haemoglobin, protein oxidation, lipid peroxidation markers, and osmotic fragility levels were increased in diabetic rats. Moreover, erythrocytes antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxide, glutathione reductase, glutathione-S-transferase, and glucose-6-phosphate dehydrogenase activities and non-enzymatic antioxidants such as vitamin C, vitamin E, reduced glutathione (GSH), and oxidized glutathione (GSSG) levels were altered. Similarly, the activities of total ATPases, Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase were also decreased in the erythrocytes of diabetic rats. Administration of SGF to STZ-induced diabetic rats reduced blood glucose and glycosylated haemoglobin levels with increased levels of insulin and haemoglobin. Moreover, SGF reversed the protein and lipid peroxidation markers, osmotic fragility, membrane-bound ATPases activities, and antioxidant status in STZ-induced diabetic rats. These results suggest that SGF could provide a protective effect on diabetes by decreasing oxidative stress-associated diabetic complications.

Dietary Choline Deprivation Exacerbates Cardiomyopathy in Streptozotocin-Induced Diabetic Adult Rats

Diabetology ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 190-204
Author(s):  
Ahmed Al-Humadi ◽  
Athina Strilakou ◽  
Hussam Al-Humadi ◽  
Rafal Al-Saigh ◽  
Emmanouel Agapitos ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Dietary Intervention ◽  
Myocardial Dysfunction ◽  
Posterior Wall ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Myocardial Inflammation ◽  
Standard Diet ◽  
Adult Rats ◽  
Male Wistar Rats

Choline (Ch) is an essential molecule of substantial importance for the optimal development and function of several biological systems. Ch deprivation has been linked with abnormal fat metabolism, insulin resistance, and myocardial dysfunction. The current study provides evidence of an exacerbation of streptozotocin-induced cardiomyopathy in adult diabetic Wistar rats by dietary Ch deprivation through the administration of a Ch-deprived diet (CDD). Twenty-four adult male Wistar rats were randomly separated into four groups: control, diabetic (DM), choline-deprived through choline-deprived diet (CD), and diabetic choline-deprived (DM + CD). After five weeks of dietary intervention, myocardium echocardiographic and histological assessments were performed. Choline-deprived diabetic rats exhibited significantly slower heart rate, significantly higher myocardial ejection velocity and left ventricle wall tension index with a concomitant significant decreased LV posterior wall thickness as compared to diabetic rats fed on a standard diet. Moreover, histopathological evidence demonstrated an exacerbation of myocardial inflammation and fibrosis associated with significant up-regulation of VEGF expression in the diabetic rat myocardium as a result of Ch deprivation. The study’s findings are of particular significance since the examined experimental approach introduces a previously uncharacterised comorbidity simulation with regards to myocardial structure and functional profiling.

scholarly journals Increase of collagen in diabetes-related traumatic ulcers after the application of liquid smoke coconut shell

2017 ◽  
Vol 50 (2) ◽  
pp. 71
Author(s):  
Meircurius Dwi Condro Surboyo ◽  
Ira Arundina ◽  
Retno Pudji Rahayu
Keyword(s):  
Diabetes Mellitus ◽  
Topical Application ◽  
Wistar Rats ◽  
Healing Process ◽  
Coconut Shell ◽  
Diabetic Patients ◽  
Process Indicator ◽  
Liquid Smoke ◽  
Patients With Diabetes ◽  
Male Wistar Rats

Background: Traumatic ulcers in patients with diabetes mellitus may experience delayed healing despite their diabetic condition being controlled. Liquid smoke coconut shell containing phenolic compounds can potentially accelerate the healing process. One healing process indicator is the increased number of fibroblasts, another being the increased amount of collagen. Purpose: This study aimed to analyze the amount of collagen in traumatic ulcers in diabetics after application of liquid smoke coconut shell. Methods: Alloxan was induced in twenty-four male Wistar rats as models of diabetes mellitus. A traumatic 10 mm ulcer was made along the labial fornix incisive inferior with a round, stainless steel blade before liquid smoke coconut shell and benzydamine hydrochloride (as the control) was administrated once a day. A biopsy of the labial fornix incisive inferior was subsequently performed after the topical application for 5 and 7 days. Histological assessment was conducted to analyze the amount of collagen by means of Masson Trichome staining. Results: Histologically, the topical application of liquid smoke coconut shell for 5 days significantly increased the amount of collagen, higher than that of benzydamine hydrochloride as the control (p=0.006) (p<0.05). Meanwhile, the topical application of liquid smoke coconut shell for 7 days made the concentration of collagen no significantly different from that of benzydamine hydrochloride as the control (p=0.156) (p>0.05). Conclusion: Liquid smoke coconut shell applied for 5 days increase the amount of collagen in traumatic ulcers in diabetic patients.

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