N2O Fluxes and Related Processes of Denitrification in Acidified Soil
Abstract In North China, high levels of N fertilizer and irrigation water are used in fields, which cause considerable N2O fluxes via several pathways, especially anaerobic denitrification. Anaerobic denitrification is regarded as an important microbial process for N2O production in soils with a low O2 level and high N and labile C availability (the typical soil conditions caused by high levels of N fertilizer and irrigation water in the field). We conducted an anaerobic incubation experiment to determine the impact of soil acidification (with a series of soil pH levels, pH 6.2, pH 7.1, and pH 8.7) on N2O source partitioning with the addition of KNO3 and glucose. Natural abundance isotope techniques and gas inhibitor technique were applied to analyze the N2O flux derived from fungal denitrification and bacterial denitrification and its isotopocule characteristics emitted from soils after the addition of NO- 3 and glucose. A mapping approach was used to obtain further insight into the N2O production processes. Our findings confirmed that soil pH strongly controlled the N2O production and reduction rates of denitrification. Soil acidification significantly increased N2O emissions varied from 0.76 mg N kg-1 for natural soil (pH 8.7), to 1.88 mg N kg-1 for pH 7.1, and to 2.35 mg N kg-1 for pH 6.2, and had a blockage effect on the reduction of N2O to N2. The addition of carbon sources promoted complete denitrification. We assumed a higher contribution of fungal denitrification to N2O production compared to total N2O emission associated with acidified soil. A promotion of the contribution of fungal denitrification-derived N2O was indeed observed with decreasing pH, increasing from 0.28 mg N kg-1 for pH 8.7 to 0.94 mg N kg-1 for pH 6.2. The addition of glucose further increased the contribution of fungal denitrification to N2O production from 0.99 mg N kg-1 for pH 8.7 to 3.66 mg N kg-1 for pH 6.2. The mapping approach provided rational results for correcting N2O reduction compared with the acetylene inhibition method. The results calculated by both methods indicated a reasonably large contribution of fungal denitrification to total N2O production in acidified soils.