scholarly journals Systematic prompting the new physiological and morphological, biological responses of modified metabolic induction process of Calendula officinalis L under heat stress with couple of supplementary nutritional applications as safety of floriculture crops

2021 ◽  
Author(s):  
waseem ahmed ◽  
Rafia Azmant ◽  
Abdul Qayyum ◽  
Ayaz Mehmood ◽  
Rasheed Ahmed
Keyword(s):  
Lipid Peroxidation ◽  
Heat Stress ◽  
Total Phenolic ◽  
Calendula Officinalis ◽  
Nitrogen And Phosphorus ◽  
Total Carotenoids ◽  
Chlorophyll Contents ◽  
Phenolic Contents ◽  
Lipid Peroxidation Assay ◽  
P Application

Abstract Purpose the objective of study to modified the metabolism of Calendula officinalis L. under heat stress couple with N and P application in two years of study. Methods the trail was conducted during peak summer season (months of May, June, and July) couple with different levels of N & P application to monitor the quality and quantity characterization of marigold (Calendula officinalis L.). The traits of three doses of N and P (0.6 g, 0.9 g, and 0.8 g, 1− 1 g) given to Calendula officinalis L.) to display the vegetative, reproductive, physiological parameters such as Malondialdehyde (MDA), Chlorophyll contents, Lipid Peroxidation Assay in leaves, Li and K contents, phytochemicals and nitrogen and phosphorus used efficacy Results showed that maximum values of Malondialdehyde (MDA), Chlorophylls, Lipid Peroxidation Assay in leaves observed followed by the alteration in Li and K measured in the month of May, June with slight differences July. The phytochemicals like total phenolic contents (84.41 mg GAE/g), total antioxidants (36.3% DPPH), total carotenoids and total flavonoids contents (16.2 and 0.9 mg/100 g) were measured by 0.9 g of Nitrogen application and followed by 1 g of Phosphorus in both years of study. The respirational changes were observed in the higher rate of P levels. The liner changes of N and P rates showed some fluctuation in heat stress months. Conclusions It was concluded that the higher doses of both N and P fertilizers were effective in controlling the heat stress and mentioned the quality of florets by various induction and biological active process.

scholarly journals Phenolics and Carotenoid Contents in the Leaves of Different Organic and Conventional Raspberry (Rubus idaeus L.) Cultivars and Their In Vitro Activity

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 458 ◽  
Author(s):  
Ponder ◽  
Hallmann
Keyword(s):  
Salicylic Acid ◽  
Rubus Idaeus ◽  
Vitro Activity ◽  
Total Phenolic ◽  
In Vitro Activity ◽  
Total Carotenoids ◽  
Chlorophyll Contents ◽  
Chlorophylls A And B ◽  
The Impact

: Raspberry leaves are a source of carotenoids and polyphenols, including ellagic acid and salicylic acid. The results of scientific research suggest that they have potential pro-health properties that contribute to human health. The aim of this study was to determine the polyphenolic and carotenoid profiles in the leaves of selected raspberry cultivars and their in vitro activity. The second aim was to determine the impact of organic and conventional farm management on the polyphenol, carotenoid, and chlorophyll contents in different raspberry cultivars: ‘Polana’, ‘Polka’, ‘Tulameen’, ‘Laszka’ and ‘Glen Ample’. Compared with conventional raspberry leaves, organic raspberry leaves were characterized by a significantly higher content of dry matter, total polyphenols, total phenolic acids, chlorogenic acid, caffeic acid, salicylic acid and quercetin-3-O-rutinoside; moreover, the organic leaves were characterized by higher antioxidant activity. Among examined cultivars, ‘Polka’ c. was characterized by the highest antioxidant status. However, raspberry leaves from conventional farms contained more total carotenoids, violaxanthin, alpha-carotene, beta-carotene, total chlorophyll and individual forms of chlorophylls: a and b.

scholarly journals Biochemical Profiling for Antioxidant and Therapeutic Potential of Pakistani Chickpea (Cicer arietinum L.) Genetic Resource

2021 ◽  
Vol 12 ◽  
Author(s):  
Saima Jameel ◽  
Amjad Hameed ◽  
Tariq Mahmud Shah
Keyword(s):  
Cicer Arietinum ◽  
Therapeutic Potential ◽  
Hydrolytic Enzymes ◽  
Total Phenolic ◽  
Enzymatic Antioxidants ◽  
Total Carotenoids ◽  
Pigment Analysis ◽  
Breeding Lines ◽  
Phenolic Contents ◽  
Seed Flour

In Pakistan, chickpeas (Cicer arietinum L.) are the largest grown legume crops, especially in desert areas. Along with an excellent source of nutrition, chickpea seeds have discernible medicinal and antioxidant characteristics. The diverse set of 90 chickpea genotypes (66 desi and 24 kabuli) were collected from different research zones in Pakistan, and seed flour was used for biochemical profiling. Genotypes were significantly different (Tukey HSD test, P < 0.05) for the traits under investigation. In non-enzymatic antioxidants, highest seed total phenolic contents (TPC) (34725 ± 275 μM/g s. wt.) was found in CM-98 (desi), ascorbic acid (AsA) (69.23 ± 2.25 μg/g s. wt.) in WH-3 (desi), and total flavonoid content (TFC) (394.98 ± 13.06 μg/mL sample) was detected in WH-11 (desi). In the class of enzymatic antioxidants, the highest seed ascorbate peroxidase (APX) (1680 ± 40 Units/g s. wt.) was detected in Tamman-2013 (kabuli), peroxidases (POD) (2564.10 ± 233.10 Units/g s. wt.) activity in CM1235/08 (desi), and superoxide dismutase (SOD) (279.76 ± 50 Units/g s. wt.) was detected in CH24/11 (desi). Highest seed catalase activity (CAT) (893 ± 50 Units/g s. wt.) and proline content (272.50 ± 20.82 μg/g s. wt.) was detected in an ICC-4951 (desi). In hydrolytic enzymes, the highest activity of esterase (37.05 μM/min/g s. wt) was found in, CH56/09(Kabuli), protease (11080 ± 10 Units/g s. wt.) in Karak-2 (desi), and α-amylase (213.02 ± 3.20 mg/g s. wt.) was observed in CH74/08 (kabuli). In other biochemical parameters, the highest seed total oxidant status (TOS) (356 ± 17.50 μM/g s. wt.) was detected in CM3457/91 (desi); malondialdehyde (MDA) content (295.74 ± 3.097 uM/g s. wt.) was observed in CM-2008 (kabuli), and total antioxidant capacity (TAC) (8.36 ± 0.082 μM/g s. wt.) was found in CM-72 (desi). In case of pigment analysis, Sheenghar-2000 (desi) depicted highest lycopene (12.579 ± 0.313 μg/g s. wt.) and total carotenoids (58.430.23 ± 0.569 μg/g s. wt.) contents. For seed therapeutic potential, the highest seed α-amylase inhibition (82.33 ± 8.06%) was observed in CM-88 (desi), while WH-1, WH-6, and ICCV-96030 (desi) depicted the highest value for seed anti-inflammatory potential (78.88 ± 0.55%). Genotypes with the highest antioxidant and therapeutic potential can be utilized as a natural antioxidant source and in breeding programs aimed at improving these traits in new breeding lines.

scholarly journals Classification of Sugarcane Genotypes Based on Heat Stress and Morphological Parameters

2013 ◽  
Vol 1 (2) ◽  
pp. 114 ◽  
Author(s):  
Syed Rizwan Abbas ◽  
Asad Hussain Shah ◽  
Aurangzeb Rao ◽  
Syed Dilnawaz Ahmad Gardazi ◽  
Syed Mubashir Sabir ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Heat Stress ◽  
Membrane Stability ◽  
Pigment Concentration ◽  
Moisture Loss ◽  
Morphological Parameters ◽  
Phenolic Contents ◽  
Relative Tolerance

Leaves of thirteen genotypes of sugarcane were treated in the oven for heat stress. The treated leaves were used for the estimation of phenolic contents and the measurement of lipid peroxidation. Minimum moisture loss is showed by CSSG-668 and maximum moisture loss showed by NSG-45. Maximum phenolic contents were observed in HSF-240 (65.7 mg GAE/100 ml) and minimum results showed by Lho 83-153 and CP-43-33 (32.98 and 33.78 mg GAE/100 ml). Lho 83-153, HSF-242 and S-2002-US-133 were showed high tolerant against heat therefor, showed higher membrane stability, maintenance of high fv/fm ratio under heat stress and lower lipid peroxidation of membranes. Hence, the relative tolerance of genotype to heat stress as reflected by its lower lipid peroxidation, higher membrane stability and pigment concentration is related to the levels of activity of its antioxidant enzymes. 

scholarly journals Adaptive Response of a Native Mediterranean Grapevine Cultivar Upon Short-Term Exposure to Drought and Heat Stress in the Context of Climate Change

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 249 ◽  
Author(s):  
Nikolaos Tzortzakis ◽  
Antonios Chrysargyris ◽  
Aziz Aziz
Keyword(s):  
Climate Change ◽  
Lipid Peroxidation ◽  
Heat Stress ◽  
Stomatal Conductance ◽  
Antioxidant Capacity ◽  
Stomatal Closure ◽  
Total Phenolic ◽  
Short Term ◽  
Short Term Exposure ◽  
The Impact

The impact of climate change on viticulture is of major importance. Several international and indigenous grapevine cultivars have been examined for their adaptive performance to drought and heat stresses. However, the underlying physiological mechanisms are not well known. In the present study we examined the short-term effects of light and moderate drought stress (DS) as well as heat stress (HS) on physiological and biochemical attributes in two grapevine cultivars: Chardonnay (international) and Xynisteri, an indigenous adapted to the specific Cypriot microclimate. Xynisteri plants exhibited decreasing leaf stomatal conductance and photosynthetic rate as well as increasing total phenols and antioxidant capacity under DS conditions. These reactions were concomitant to a rapid accumulation of hydrogen peroxide and lipid peroxidation in leaves, associated to an increase of the antioxidant superoxide dismutase activity. However, Chardonnay plants did not exhibit the same responses as Xynisteri against DS (i.e., stomatal closure, total phenolics and antioxidants) over the first four days. Additionally, Chardonnay showed leaf damage with increased lipid peroxidation levels and activation of catalase and peroxidase. Interestingly, HS increased leaf stomatal conductance and decreased total phenolic content, flavonoids and antioxidant capacity in Chardonnay after 20 d of stress. In both cultivars, HS had milder effects compared to DS, and again Xynisteri showed better performance than Chardonnay in terms of damage index and antioxidative activities. Overall, Xynisteri adaptation to DS and HS was higher than that of Chardonnay, and both cultivars reacted more to the short-term DS than to HS. Although the Xynisteri performances under stress conditions provide an important resource for adaptation to stressful conditions, the impacts on earliness, yield and grape quality remain to be explored.

scholarly journals Total Phenolic Contents and Antioxidant Capacity of Aqueous Extract from Pituranthos scoparius (Coss. & Dur.) Growing in Algeria

2020 ◽  
Vol 10 (3) ◽  
pp. 125-127
Author(s):  
Ahlem Karbab ◽  
Kamel Mokhnache ◽  
Lekhmici Arrar ◽  
Noureddine Charef
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Aqueous Extract ◽  
Health Benefit ◽  
Natural Antioxidants ◽  
Antioxidant Property ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Dpph Scavenging

This study aims to estimate the total phenolics, flavonoids and tannins contents then to investigate both in vitro antioxidant capacity models of aqueous extract (AqE) from the stems of Pituranthos scoparius, growing in Algeria. Total polyphenol contents were determined using Folin Ciocalteu's reagent; flavonoids were quantified employing the AlCl3 and method tannins using haemoglobin precipitation test. The in vitro antioxidant property was assessed by DPPH-scavenging radical and lipid peroxidation assays. The results revealed that aqueous extract presented a high total phenolic and tannins contents with values of 150.89 ± 0.68 mg GAE (gallic acid equivalent)/g and 71.24 ± 0.09 mg TAE (tannic acid equivalent)/g dry extract, respectively. This extract show an essential effect toward DPPH-scavenging assay and lipid peroxidation inhibition with 96.19 ± 0.00 µg/mL, 91.53 ± 0.98 %, respectively. This study indicates that the aqueous extract from Pituranthos sciparius has potent antioxidant effects and may prove to be of latent health benefit as well as supplementary sources for natural antioxidants drugs. Keywords: Pituranthos scoparius, aqueous extract, antioxidant activity, phenolic compounds.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

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