scholarly journals Hydroxytyrosol Effectively Improves The Quality of Pig Sperm at 17 °C

2021 ◽  
Author(s):  
Dong Li ◽  
Wenyu Zhang ◽  
Xuekai Tian ◽  
Zitong Xiao ◽  
Xin Zhao ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Sperm Quality ◽  
Active Oxygen Species ◽  
Membrane Damage ◽  
Treated Group ◽  
Control Group ◽  
Sequencing Analysis ◽  
Oxygen Species ◽  
Sperm Protein

Abstract Background: Artificial insemination technology is the production technology used in more than 99% of the world's pig farms. As the frozen sperm damage is very serious, the preservation of pig sperm is mainly carried out at 17°C. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen species and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol group (optimum concentration). Ultimately, we selected the control group and the 120 μmol/L hydroxytyrosol group to test the effect of artificial insemination.Results: The results of our research showed that during storage of pig sperm at 17 ℃, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated group were significantly improved (P < 0.05). Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm quality. Besides, the significant increase in sow pregnancy rate and piglet birth weight also confirmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrate that the addition of hydroxytyrosol to the diluent can improve the quality of pig sperm and the efficiency of artificial insemination.

scholarly journals Hydroxytyrosol Effectively Improves The Quality of Pig Sperm at 17 ℃

2021 ◽  
Author(s):  
Dong Li ◽  
Wenyu Zhang ◽  
Xuekai Tian ◽  
Yulin He ◽  
Zitong Xiao ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Sperm Quality ◽  
Active Oxygen Species ◽  
Membrane Damage ◽  
Treated Group ◽  
Control Group ◽  
Sequencing Analysis ◽  
Oxygen Species ◽  
Sperm Protein

Abstract Background: Artificial insemination technology is the production technology used in more than 99% of the world's pig farms. As the frozen sperm damage is very serious, the preservation of pig sperm is mainly carried out at 17°C. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen species and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol group (optimum concentration). Ultimately, we selected the control group and the 120 μmol/L hydroxytyrosol group to test the effect of artificial insemination.Results: The results of our research showed that during storage of pig sperm at 17 ℃, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated group was significantly improved (P < 0.05). Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm quality. Besides, the significant increase in sow pregnancy rate and piglet birth weight also confirmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrate that the addition of hydroxytyrosol to the diluent could improve the quality of pig sperm and the efficiency of artificial insemination.

Hydroxytyrosol effectively improves the quality of pig sperm at room temperature

2020 ◽  
Author(s):  
Wenuyu Zhang ◽  
Dong Li ◽  
Xueka Tian ◽  
Yulin He ◽  
Zitong Xiao ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Room Temperature ◽  
Sperm Quality ◽  
Membrane Damage ◽  
Treated Group ◽  
Control Group ◽  
Sequencing Analysis ◽  
Oxygen Species ◽  
Boar Semen

Abstract Background: Artificial insemination technology is the production technology used in more than 99% of the world's pig farms. Preservation of semen is mainly conducted at room temperature (17°C), during which the accumulation of reactive oxygen species (ROS). In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of boar semen at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, Proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol (optimum concentration) group after three days’ storage. Ultimately, we selected the control group and the addition of 120 μmol/L hydroxytyrosol group to test the effect of artificial insemination.Results: During storage of pig semen at room temperature, the sperm quality of the hydroxytyrosol-treated group was significantly improved (P < 0.05). Besides, its antioxidant capacity was also significantly improved (P < 0.05). Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm cell quality. The significant increase in sow pregnancy rate and the increase in piglet birth weight also confirmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrated that the addition of hydroxytyrosol to the diluent can not only improve the quality of pig sperm, but also has an irreplaceable role in improving the efficiency of artificial insemination.

Vitamin E ameliorates disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine and convicine of Vicia faba

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Khaled M.M. Koriem ◽  
Mahmoud S.S. Arbid
Keyword(s):  
Vitamin E ◽  
Vicia Faba ◽  
Sperm Quality ◽  
Treated Group ◽  
Male Rats ◽  
Control Group ◽  
Content Type ◽  
Paraffin Oil ◽  
Male Genital

Purpose This paper aims to design to evaluate the protective effect of vitamin E to ameliorate the disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine (V) and convicine (C) of Vicia faba. Design/methodology/approach Forty male albino rats were divided into five equal groups; control, paraffin oil, V (400 mg/kg) C (150 mg/kg)-treated group, vitamin E (100 mg/kg) + VC-treated group, and vitamin E (200 mg/kg) + VC-treated groups which injected intraperioneally (IP) with 0.5-ml saline, 0.5-ml paraffin oil,V (400 mg/kg) and C (150 mg/kg) of Vicia faba, vitamin E (100 mg/kg) + VC-treated groups, and Vitamin E(200 mg/kg) + VC-treated groups, respectively. Blood and testicular tissue were obtained after one month of the study. The male genital organs were calculated. Testosterone (Ts), luteinizing hormone (LH), follicle stimulating hormone (FSH), dehydroepiandrosterone sulfate (DHEA-SO4), sex hormone binding globulin (SHBG),?-glutamyl transpeptidase (?-GT), glucose-6-phosphate dehydrogenase (G6PD), 3ß-hydroxysteroid dehydrogenase (3ßHSD), lactate dehydrogenase (LDH), spermatozoa concentration, percent of mortality and abnormal sperms were evaluated. Findings The VC-treated group showed significant decrease (p < 0.01) in Ts, DHEA-SO4, G6PD, spermatozoa number and mortality percent, as well as, the male genital organs (testes, epidydemis, seminal vesicle, prostate and vasa deferentia) while significant increase (p < 0.01) was found in LH, FSH, SHBG, LDH, ?-GT, sperms monoclonal Ki-67, and abnormal spermatocytes levels compared with control group. Vitamin E co-injection with VC-treated group returned all these parameters to the normal values. The higher dose of vitamin E (200 mg/kg) was more effect than the lower dose (100 mg/kg). Originality/value Vicia faba contains V and C glycosides. The V and C glycosides in Vicia faba are hydrolyzed by intestinal microflora to aglycones divicine and isouramil, respectively. Divicine and isouramil are highly reactive compounds generating free radicals where divicine and isouramil are the main factors of favism. The V and C glycosides induced disturbances in testosterone pathway and sperm quality of male rats and vitamin E ameliorates these disturbances.

scholarly journals Pengaruh pemberian ekstrak jintan hitam (Nigella sativa) terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang terpapar asap rokok

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Irene M. A. Rumampuk ◽  
Lydia Tendean ◽  
Grace L. A. Turalaki
Keyword(s):  
Reactive Oxygen Species ◽  
Body Weight ◽  
Cigarette Smoke ◽  
Nigella Sativa ◽  
Sperm Quality ◽  
Reactive Oxygen ◽  
Control Group ◽  
Continuous Exposure ◽  
Oxygen Species

Abstract: Continuous exposure to cigarette smoke has been reported as the number one cause of infertility in men. Cigarette smoke increases the production of reactive oxygen species (ROS) thereby causing infertility in men. Nigella sativa contains antioxidant that may decrease ROS level in hope to improve infertility. This 50 days study is conducted to see the effect of Nigella sativa extract to the sperm quality of nine wistar rats that have been exposed by cigarette smoke. The rats were divided into three groups of three: the control group and the group treated with Nigella sativa administered orally at the dose of 200 mg kg body weight and 400 mg/kg body weight. Sperm quality was observed by overlooking at the concentration, motility, and morphology of each group. The mean data of each group was obtained and tested for significance using Independent Samples T-Test. This study found the comparison of both motility (p=0,006) and morphology (p=0,000) between control group and the group with the dose of 200 mg/kg body weight to be significant, as well as the comparison of motility (p=0,001) and morphology (p=0,000) between the control group and the group with the dose of 400 mg/kg body weight.Keywords: sperm quality, nigella sativa, smoking, cigaretteAbstrak: Paparan secara terus menerus terhadap asap rokok merupakan penyebab infertilitas utama pada pria. Asap rokok dapat meningkatkan Reactive oxygen species (ROS) yang selanjutnya dapat menyebabkan infertilitas. Nigella sativa mengandung antioksidan yang dapat menunrunkan jumlah ROS dan diharapkan untuk dapat mengkoreksi infertilitas. Penelitian yang dilaksanakan selama 50 hari ini bertujuan untuk melihat pengaruh dari ekstrak Nigella sativa terhadap kualitas spermatozoa sembilan tikus wistar yang terpapar asap rokok. Tikus dibagi menjadi tiga kelompok masing-masing terdiri atas tiga tikus; terdapat kelompok kontrol dan kelompok yang diberi ekstrak Nigella sativa dengan dosis 200 mg/kg BB dan 400 mg/kg BB. Kualitas spermatozoa dinilai berdasarkan konsentrasi, motilitas dan morfologi dari masing-masing kelompok perlakuan. Data rerata dari tiap kelompok perlakuan diambil dan di diuji signifikans menggunakan uji T-independen. Penelitian ini mendapati perbandingan baik motilitas (p=0,006) ataupun morfologi (p=0,000) dari kelompok kontrol dan perlakuan dengan dosis 200 mg/kg BB dibilang signifikan, sama halnya dengan perbandingan antara motilitas (0,001) dan morfologi (0,000) dari kelompok kontrol dan perlakuan dengan dosis 400 mg/kg BB.Kata kunci: kualitas sperma, jintan hitam, rokok

The Potential Effect of Mucuna pruriens Seed Extract on Sperm Quality experimental study on mice exposed to cigarette smoke

2021 ◽  
Vol 20 (4) ◽  
pp. 768-773
Author(s):  
Meidona Nurul Milla ◽  
Yani Istadi ◽  
Vania Shaula ◽  
Deastri Anjeas Wari ◽  
Chntyia Dwi Cahyani Puspitasari ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cigarette Smoke ◽  
Sperm Quality ◽  
Medical Science ◽  
Seed Extract ◽  
Reproductive Age ◽  
Testicular Atrophy ◽  
Mucuna Pruriens ◽  
Control Group

Background: Infertility has been more common problems among couple of reproductive age. One of the factors causing this disorder is unhealthy environmental factors including exposure to cigarette smoke. Polynuclear aromatic hydrocarbons (PAHs) in cigarette smoke can cause testicular atrophy, while the free radicals can inhibit the stages of spermatogenesis, and nicotine in cigarettes affects the brain dopamine levels affecting the levels of GnRH, and subsequently affect the levels of FSH and LH needed in spermatogenesis. The use of Mucuna pruriens seed extract containing antioxidants and L-dopa is expected to improve the quality of sperm after exposure to cigarette smoke. Objective: This study aimed to determine the effect of Mucuna pruriens seed extract on the sperm quality in mice exposed to cigarette smoke. Methods: This study was an experimental study with a post test only control group design. A total of 20 mice were divided into 4 groups of five mice each. All groups were exposed to cigarette smoke. Group 1 was the negative control exposed to cigarette smoke. Groups 2, 3, 4 were exposed to cigarettes smoke and given Mucuna pruriens seed extracts at the dose of 250; 300; and 350 mg/Kg BW/day. Parameters of sperm quality included concentration, morphology, motility and viability. Results: Post hoc tests showed there were significant differences among treatment groups. Conclusion: the administration of Mucuna pruriens seed extract affects the sperm quality of BALB/c mice exposed to cigarettes smoke. Bangladesh Journal of Medical Science Vol.20(4) 2021 p.768-773

scholarly journals KUALITAS SPERMA EJAKULAT PEJANTAN AYAM KUKUAK BALENGGEK PADA PENGANDANGAN TUNGGAL TERISOLASI (Ejaculated Sperm Quality of Isolated Single Caging of Balenggek Chickens)

2017 ◽  
Vol 18 (01) ◽  
pp. 40-45
Author(s):  
Ramadhan Sumarmin ◽  
Elsa Yuniarti ◽  
Abdul Razak
Keyword(s):  
Sperm Quality ◽  
Control Group ◽  
Single Cage ◽  
Counting Chamber

The Balenggek chicken is a long crower type fowl. This fowl have isolated and caging in single cage for years to result the long crower sound.This study was carried out to identify the influence of fed to the ejaculate sperm quality of balenggek chickens. First, the sperm were collected from isolated caging of Balenggek fowl more or less than three years and un-isolated caging of Balenggek fowl as control group. Second, the sperm were collected from isolated caging of Balenggek fowl more or less than three years and un-isolated caging of Balenggek fowl as control group, after treated by 124 ComFed for three months. The sperm were collected by massages methods and analyze with counting chamber of Improve Neubauer. Slides of sperm were stained by Eosin. Variables observed were sperm ejaculated, colours, and sperm consistency. The results shows that the sperm quality of Balenggek fowl increased (from 2.5 billion sperm/ml to 3.5 billion sperm/ml) significantly higher (p<0.05) in isolated caging fowl with reschedule feeding. Sperm color and its consistency were increased from + (c quality) to ++ (b quality). It can conclude that single caging could decreased the ejaculated sperm of Balengggek chickens quality and it. could reveal increased after it treated by 124 ConFed.

scholarly journals Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

2020 ◽  
Vol 13 (5) ◽  
pp. 947-955
Author(s):  
Zulfi Nur Amrina Rosyada ◽  
Mokhamad Fakhrul Ulum ◽  
Ligaya I. T. A. Tumbelaka ◽  
Bambang Purwantara
Keyword(s):  
Artificial Insemination ◽  
Sperm Quality ◽  
High Fertility ◽  
Fertility Level ◽  
Sperm Protein ◽  
Sperm Proteins ◽  
Frozen Semen ◽  
Sds Page ◽  
Microscopic Evaluation ◽  
Holstein Bulls

Background and Aim: Holstein cows and heifers are widely bred in Indonesia by artificial insemination (AI) to increase population and milk production. Sperm fertility is modulated by genetic factors, but the analysis of sperm quality is still based on macro- and microscopic characteristics. This study aimed to analyze both sperm quality and proteins of Holstein bulls at different fertility levels. Materials and Methods: The frozen semen samples were collected from the Indonesia National AI Center. They were classified based on the reproductive efficiency data and were grouped into high fertile (HF) and low fertile (LF). Sperm qualities were evaluated by microscopic evaluation. The Holstein sperm proteins were extracted using phenylmethanesulfonyl fluoride as a protease inhibitor and the benzidine detergent extraction method. Discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was conducted to analyze the molecular weights (MWs) of the sperm proteins. The data obtained were analyzed by a t-test using the one-factor bull fertility level, and Spearman's correlation analysis was used to identify the correlation between the sperm microscopic evaluation parameters and protein bands. Results: The sperm motility post-freeze thawing was not significantly different between the HF and LF (p>0.05). The HF level had a higher percentage of viability, intact plasma membrane integrity, and intact acrosomes than the LF (p<0.05). Five protein bands were found in the SDS-PAGE of sperm proteins of Holstein bulls with different concentrations. Sperm proteins with MWs of 17.51 kDa, 14.87 kDa, 33.71 kDa, and 41.97 kDa were abundant in the Holstein bulls with an HF level, while 55 kDa proteins were abundant in the LF level of Holstein bulls. The sperm of Holstein bulls in the HF level contained proteins of about 33.71 kDa that were not detected in the LF. Conclusion: The sperm protein with a molecular weight of 33.71 kDa was predicted to be a specific protein biomarker that influences bull fertility. Sperm fertilization abilities were also determined by the sperm proteins, the morphology of sperm acrosomes, and the quality of plasma membranes. This method can be used to select bulls with high fertility to increase the population of Holstein bulls.

scholarly journals The Effect of Adding Different Levels of Curcumin and Its Nanoparticles to Extender on Post-Thaw Quality of Cryopreserved Rabbit Sperm

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1508 ◽  
Author(s):  
Sameh Abdelnour ◽  
Mahmoud Hassan ◽  
Amer Mohammed ◽  
Ahmad Alhimaidi ◽  
Naif Al-Gabri ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Positive Influence ◽  
Control Group ◽  
Curcumin Nanoparticles ◽  
Total Antioxidant ◽  
Semen Extender ◽  
Apoptosis Process ◽  
Structural Levels

The cryopreservation process adversely affects sperm function and quality traits, causing some changes at biochemical and structural levels, due to mechanical, thermal, osmotic, and oxidative damage. Supplementation with curcumin nanoparticles could prevent and even revert this effect and could enhance the post/thawed sperm quality in the rabbit. The study amid to explore the effect of curcumin (CU) and curcumin nanoparticles (CUNPs) supplementation in semen extender on post/thawed rabbit sperm quality. Twelve fertile, healthy rabbit bucks were included, and the ejaculates were collected using artificial vaginas. Rabbit pooled semen was cryopreserved in tris-yolk fructose (TYF) extender without any supplement (control group) or extender supplemented with CU at levels of 0.5, 1 or 1.5 µg/mL (CU0.5, CU1.0, and CU1.5, respectively) or CUNPs at levels of 0.5, 1, 1.5 (CUNPs0.5, CUNPs1.0, and CUNPs1.5, respectively) and was packed in straws (0.25 mL) and stored in liquid nitrogen (−196 °C). Results revealed that CUNPs1.5 had a positive influence (p < 0.05) on post-thawing sperm progressive motility, viability, and membrane integrity as compared with the other groups. Percentages of dead sperm, abnormalities, early apoptotic, apoptotic, and necrotic sperm cells reduced (p < 0.05) in CUNPs1.5 as compared to other treatments. Using 1.5 µg/mL of CUNPs significantly improved total antioxidant capacity (TAC), GPx, while MDA and POC reduced (p < 0.05) in CU1.5 in comparison with other groups. SOD values were enhanced (p < 0.05) in CUNPs1.0 and CUNPs1.5 in relation with other treatments. Conclusively, the addition of curcumin and its nanoparticles to the extender can improve the post-thawed quality of rabbit sperm via redox signaling and reduce the apoptosis process.

Supplementation of kaempferol to in vitro maturation medium regulates oxidative stress and enhances subsequent embryonic development in vitro

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 59-64
Author(s):  
Yuhan Zhao ◽  
Yongnan Xu ◽  
Yinghua Li ◽  
Qingguo Jin ◽  
Jingyu Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Treated Group ◽  
Control Group ◽  
Oxygen Species

SummaryKaempferol (KAE) is one of the most common dietary flavonols possessing biological activities such as anticancer, anti-inflammatory and antioxidant effects. Although previous studies have reported the biological activity of KAE on a variety of cells, it is not clear whether KAE plays a similar role in oocyte and embryo in vitro culture systems. This study investigated the effect of KAE addition to in vitro maturation on the antioxidant capacity of embryos in porcine oocytes after parthenogenetic activation. The effects of kaempferol on oocyte quality in porcine oocytes were studied based on the expression of related genes, reactive oxygen species, glutathione and mitochondrial membrane potential as criteria. The rate of blastocyst formation was significantly higher in oocytes treated with 0.1 µm KAE than in control oocytes. The mRNA level of the apoptosis-related gene Caspase-3 was significantly lower in the blastocysts derived from KAE-treated oocytes than in the control group and the mRNA expression of the embryo development-related genes COX2 and SOX2 was significantly increased in the KAE-treated group compared with that in the control group. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased after KAE treatment. Mitochondrial membrane potential (ΔΨm) was increased and the activity of Caspase-3 was significantly decreased in the KAE-treated group compared with that in the control group. Taken together, these results suggested that KAE is beneficial for the improvement of embryo development by inhibiting oxidative stress in porcine oocytes.

Single Dose of Magnesium Sulfate as an Adjuvant to General Anesthesia Improves Pain Control, Discomfort and Quality of Sleep Postoperatively

2016 ◽  
Vol 70 (3) ◽  
pp. 145-147
Author(s):  
Biljana Kuzmanovska ◽  
Marija Donevska Tolevska ◽  
Andrijan Kartalov ◽  
Marija Jovanovski Srceva ◽  
Mirjana Shosholcheva ◽  
...  
Keyword(s):  
Single Dose ◽  
Postoperative Pain ◽  
General Anesthesia ◽  
Magnesium Sulfate ◽  
Treated Group ◽  
Control Group ◽  
Quality Of Sleep ◽  
Analgesic Consumption ◽  
Pain At Rest

Abstract Introduction. As a N-methyl-D-aspartate receptors antagonist, magnesium may play a role in prevention of pain. The aim of this study was to assess the effects of single dose magnesium sulfate preoperatively on postoperative pain scores, discomfort and quality of sleep. Methods. Forty patients scheduled for elective laparoscopic cholecystectomy were randomized in two groups. Group A received 20 mg/kg magnesium sulfate after the anesthesia induction, prior to surgical incision, and Group B was the control group. Assessment was made for pain at rest in both groups according to VAS (0-10), analgesic consumption, discomfort and quality of sleep during first 48 hours postoperatively. Resultѕ. Compared to control group, magnesium-treated patients had lower postoperative pain at rest according to VAS score (p< 0.05) and consumed less analgesic drugs during the first 48 hours (p<0.05). The magnesium-treated group experienced less discomfort during the first and the second postoperative day. The magnesium-treated group reported no changes in sleeping pattern compared to preoperative sleeping pattern, while the control group showed an increase in insomnia during the first and the second postoperative night, compared to that preoperatively. Conclusion. Perioperative use of magnesium sulfate as an adjuvant to general anesthesia is associated with lower postoperative pain, less analgesic consumption, less discomfort and better sleep in the postoperative period.

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