scholarly journals Biosynthesis of Silver Nanoparticles and Their Biocontrol Potentials Against Aspergillus Niger and Fusarium Udum

2021 ◽  
Author(s):  
Aneesh Sikka ◽  
Triveni Sodimalla ◽  
NAGARAJU YALAVARTHI
Keyword(s):  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Environmentally Benign ◽  
Fusarium Udum ◽  
Spherical Mean ◽  
Transmission Electron ◽  
Mean Size ◽  
The Media ◽  
Zeta Potential Analysis

Abstract Silver nanoparticles can be biosynthesized from bacteria, fungi, and plant extracts but due to their ability to synthesize nanoparticles in varying sizes and shapes at ease, bacterial has drawn interest. Bacterial based biosynthesis is effective, inexpensive, and simple thus, Pseudomonas fluorescence cell filtrates were used to synthesize silver nanoparticles in the present study. The chromatic shifts (yellow to brown) in the media after overnight incubation and the absorption of UV-Vis spectra at 420 nm confirmed the biosynthesis of AgNP’s. Besides that, the SPR analysis of AgNP’s showed a 400–500 nm band width, supporting the formation of silver nanoparticles and their small size with a uniform shape. AgNP’s transmission electron microscopy (TEM) images confirmed their shape as quasi spherical, mean size as 30 nm and anisotropy. From the Zeta potential analysis (-42.7 mV at pH = 7 with a single peak), highly repulsive nature of nanoparticles was confirmed. On the other hand, bio-fabricated silver nanoparticles were tested for antifungal activity against Fusarium udum and Aspergillus niger under in vitro conditions. At 150 ppm concentration of AgNP’s, Fusarium udum and Aspergillus niger were inhibited up to 100 and 80.50 %, respectively. In conclusion, synthesis of nanoparticle with aqueous Pseudomonas fluorescence extract is simple and environmentally benign.

scholarly journals Mucoadhesive Nanoparticles for Drug Delivery to the Anterior Eye

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1400
Author(s):  
Nicole Mangiacotte ◽  
Graeme Prosperi-Porta ◽  
Lina Liu ◽  
Megan Dodd ◽  
Heather Sheardown
Keyword(s):  
Morphological Analysis ◽  
Phenylboronic Acid ◽  
Spherical Particles ◽  
Target Tissue ◽  
Hydroxyethyl Methacrylate ◽  
Potential Analysis ◽  
Transmission Electron ◽  
Nanoparticle System ◽  
Zeta Potential Analysis

While the use of topical drops for the delivery of drugs to the anterior of the eye is well accepted, it is far from efficient with as little as 5% of the drug instilled on the eye actually reaching the target tissue. The ability to prolong the residence time on the eye is desirable. Based on the acceptability of 2-hydroxyethyl methacrylate based polymers in contact lens applications, the current work focuses on the development of a poly(2-hydroxyethyl methacrylate (HEMA)) nanoparticle system. The particles were modified to allow for degradation and to permit mucoadhesion. Size and morphological analysis of the final polymer products showed that nano-sized, spherical particles were produced. FTIR spectra demonstrated that the nanoparticles comprised poly(HEMA) and that 3-(acrylamido)phenylboronic acid (3AAPBA), as a mucoadhesive, was successfully incorporated. Degradation of nanoparticles containing N,N′-bis(acryloyl)cystamine (BAC) after incubation with DL-dithiothreitol (DTT) was confirmed by a decrease in turbidity and through transmission electron microscopy (TEM). Nanoparticle mucoadhesion was shown through an in-vitro zeta potential analysis.

scholarly journals Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Essential Oils from AgNPs and AuNPs Elicited Lavandula angustifolia In Vitro Cultures

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 606 ◽  
Author(s):  
Aneta Wesołowska ◽  
Paula Jadczak ◽  
Danuta Kulpa ◽  
Włodzimierz Przewodowski
Keyword(s):  
Molecular Weight ◽  
Silver Nanoparticles ◽  
Essential Oils ◽  
Culture Media ◽  
In Vitro Cultures ◽  
Gas Chromatography Mass Spectrometry ◽  
Lavandula Angustifolia ◽  
Gold And Silver Nanoparticles ◽  
The Media

The aim of this study was to determine how the addition of gold and silver nanoparticles to culture media affects the composition of essential oils extracted from Lavandula angustifolia propagated on MS media with the addition of 10 and 50 mg·dm−3 of gold (24.2 ± 2.4 nm) and silver (27.5 ± 4.8 nm) nanocolloids. The oil extracted from the lavender tissues propagated on the medium with 10 mg·dm−3 AgNPs (silver nanoparticles) differed the most with respect to the control; oil-10 compounds were not found at all, and 13 others were detected which were not present in the control oil. The addition of AuNPs (gold nanoparticles) and AgNPs to the media resulted in a decrease of lower molecular weight compounds (e.g., α- and β-pinene, camphene, δ-3-carene, p-cymene, 1,8-cineole, trans-pinocarveol, camphoriborneol), which were replaced by those of a higher molecular weight (τ- and α-cadinol 9-cedranone, cadalene, α-bisabolol, cis-14-nor-muurol-5-en-4-one, (E,E)-farnesol).

Mythology Merges with Technology for Majestic Production of Silver Nanoparticles: Rudraksha Enabled

2012 ◽  
Vol 585 ◽  
pp. 144-148
Author(s):  
Poushpi Dwivedi ◽  
S.S. Narvi ◽  
R.P. Tewari
Keyword(s):  
Silver Nanoparticles ◽  
Vital Role ◽  
Environmentally Benign ◽  
Size Analysis ◽  
Visible Spectroscopy ◽  
Efficient Manner ◽  
X Ray ◽  
Transmission Electron ◽  
Uv Visible ◽  
Cost Efficient

In this nanoregime attempts to bring forth nanoparticles and nanomaterials are myriads, with there interesting and demanding applications in almost every field. Today the field of nanoscience has bloomed with the confluence of nanotechnology with material science, biology, biotechnology and medicine and the need for nanotechnology will only increase as miniaturization becomes extremely important in various arrays of life. Since time immemorial silver nanoparticles have been extensively used for hygienic and healing purposes, and even until most recently, it has indispensible vital role especially in the biomedical arena. Thus in an attempt to generate silver nanoparticles employing green, environmentally benign route, we have designed to converge mythology with technology, with the mystical production of silver nanoparticles, enabled by the blueberry beads of the plant Elaeocarpus granitrus Roxb., the Rudraksha. This non-degradable bead does not disintegrate, but retains the potentiality, even after unlimited production of silver nanoparticles, assisting infinite times. The extremely cost-efficient nanoparticles thus developed in a superiorly efficient manner were characterized through different techniques; like UV/visible spectroscopy, PL spectroscopy, transmission electron microscopy, energy dispersive X-ray analysis and nanoparticle size analysis.

Evaluation of anthelmintic activity of biologically synthesized silver nanoparticles against the gastrointestinal nematode, Haemonchus contortus

2016 ◽  
Vol 91 (4) ◽  
pp. 454-461 ◽  
Author(s):  
R.S. Tomar ◽  
S. Preet
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematode ◽  
Experimental Plant ◽  
Egg Hatch ◽  
Transmission Electron ◽  
Motility Inhibition

AbstractThe present study focuses on the in vitro anthelmintic activity of silver nanoparticles (AgNPs) synthesized using the aqueous extract of Azadirachta indica against Haemonchus contortus. The synthesized AgNPs were characterized by ultraviolet–visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD) studies. The UV-Vis spectrum exhibited a sharp peak at 420 nm, which was validated by electron microscopy, indicating the preparation of spherical nanoparticles measuring 15–25 nm in size. The in vitro study was based on an egg hatch assay (EHA) and adult motility inhibition assays. Six concentrations of AgNPs were used for EHA, ranging from 0.00001 to 1.0 μg/ml, and a range of 1–25 μg/ml was used for adult worms. The highest concentration induced 85 ± 2.89% egg hatch inhibition. The IC50 value for EHA was 0.001 μg/ml, whereas in vitro adult H. contortus motility inhibition was produced at 7.89 μg/ml (LC50). The effectiveness of A. indica leaf extract (aqueous) was also evaluated, which showed an IC50 value for EHA of 115.67 μg/ml, while the LC50 against adult H. contortus was 588.54 μg/ml. The overall findings of the present study show that the experimental plant extract contains reducing properties for the synthesis of AgNPs which, in turn, showed potent anthelmintic properties. This is the first report where AgNPs have been tested for their anthelmintic properties in an in vitro model.

scholarly journals In Vitro Antioxidant, Antitumor and Photocatalytic Activities of Silver Nanoparticles Synthesized Using Equisetum Species: A Green Approach

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7325
Author(s):  
Denisa Batir-Marin ◽  
Cornelia Mircea ◽  
Monica Boev ◽  
Ana Flavia Burlec ◽  
Andreia Corciova ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Photocatalytic Activity ◽  
Organic Dyes ◽  
Silver Ions ◽  
Green Approach ◽  
Transmission Electron ◽  
Biosynthesized Agnps ◽  
The Stability ◽  
Ld50 Value

The ethanolic extracts of three Equisetum species (E. pratense Ehrh., E. sylvaticum L. and E. telmateia Ehrh.) were used to reduce silver ions to silver nanoparticles (AgNPs). The synthesized AgNPs were characterized using UV-Vis spectrophotometry, Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive X-ray (EDX), Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) measurements. FTIR data revealed the functional groups of biomolecules involved in AgNPs synthesis, such as O-H, C-H, C=O, C-O, and C-C. EDX spectroscopy was used to highlight the presence of silver, while DLS spectroscopy provided information on the mean diameter of AgNPs, that ranged from 74.4 to 314 nm. The negative Zeta potential values (−23.76 for Ep–AgNPs, −29.54 for Es–AgNPs and −20.72 for Et–AgNPs) indicate the stability of the obtained colloidal solution. The study also focused on establishing the photocatalytic activity of AgNPs, which is an important aspect in terms of removing organic dyes from the environment. The best photocatalytic activity was observed for AgNPs obtained from E. telmateia, which degraded malachite green in a proportion of 97.9%. The antioxidant action of the three AgNPs samples was highlighted comparatively through four tests, with the best overall antioxidant capacity being observed for AgNPs obtained using E. sylvaticum. Moreover, the biosynthesized AgNPs showed promising cytotoxic efficacy against cancerous cell line MG63, the AgNPs obtained from E. sylvaticum L. providing the best result, with a LD50 value around 1.5 mg/mL.

scholarly journals Investigation of the Larvicidal Potential of Silver Nanoparticles againstCulex quinquefasciatus: A Case of a Ubiquitous Weed as a Useful Bioresource

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Elijah T. Adesuji ◽  
Omolara O. Oluwaniyi ◽  
Haleemat I. Adegoke ◽  
Roshila Moodley ◽  
Ayomide H. Labulo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Volume Ratio ◽  
Average Diameter ◽  
Environmentally Benign ◽  
X Ray ◽  
Transmission Electron ◽  
Aqueous Leaf Extract ◽  
Temperature Time ◽  
Filariasis Vector

Biosynthesized silver nanoparticles (AgNPs) usingCassia hirsutaaqueous leaf extract were reported in this study. The synthesis was optimized by measuring various parameters such as temperature, time, volume ratio, and concentration. The surface plasmon resonance at 440 nm for 30°C and 420 nm for both 50°C and 70°C measured using the UV-Vis spectrophotometer confirmed the formation of AgNPs synthesized usingC.hirsuta(CAgNPs). The functional groups responsible for the reduction and stabilization of the NPs were identified using Fourier Transform Infrared (FTIR). The morphology, size, and elemental composition of the NPs were obtained using scanning electron microscope (SEM), transmission electron microscope (TEM), and energy dispersive X-ray spectroscopy (EDX). X-ray diffractometer was used to identify the phases and crystallinity of CAgNPs. Crystalline spherical NPs with average diameter of 6.9 ± 0.1 nm were successfully synthesized. The thermal analysis of CAgNPs was observed from DSC-TGA. The larvicidal results against the different larva instar stage ofCulex quinquefasciatusgave LC50= 4.43 ppm and LC90= 8.37 ppm. This is the first study on the synthesis of AgNPs usingC.hirsutaand its application against lymphatic filariasis vector. Hence, it is suggested that theC.hirsutasynthesized AgNPs would be environmentally benign in biological control of mosquito.

scholarly journals Bio-Based Nanoparticles as a Carrier of β-Carotene: Production, Characterisation and In Vitro Gastrointestinal Digestion

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4497
Author(s):  
Beatriz S. Afonso ◽  
Ana G. Azevedo ◽  
Catarina Gonçalves ◽  
Isabel R. Amado ◽  
Eugénio C. Ferreira ◽  
...  
Keyword(s):  
Encapsulation Efficiency ◽  
In Vitro Digestion ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Mean Size ◽  
Average Size ◽  
Size Controlled ◽  
Β Carotene

β-carotene loaded bio-based nanoparticles (NPs) were produced by the solvent-displacement method using two polymers: zein and ethylcellulose. The production of NPs was optimised through an experimental design and characterised in terms of average size and polydispersity index. The processing conditions that allowed to obtain NPs (<100 nm) were used for β-carotene encapsulation. Then β-carotene loaded NPs were characterised in terms of zeta potential and encapsulation efficiency. Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction analysis were performed for further morphological and chemical characterisation. In the end, a static in vitro digestion following the INFOGEST protocol was performed and the bioaccessibility of β-carotene encapsulated in both NPs was determined. Results show that the best conditions for a size-controlled production with a narrow size distribution are lower polymer concentrations and higher antisolvent concentrations. The encapsulation of β-carotene in ethylcellulose NPs resulted in nanoparticles with a mean average size of 60 ± 9 nm and encapsulation efficiency of 74 ± 2%. β-carotene loaded zein-based NPs resulted in a mean size of 83 ± 8 nm and encapsulation efficiency of 93 ± 4%. Results obtained from the in vitro digestion showed that β-carotene bioaccessibility when encapsulated in zein NPs is 37 ± 1%, which is higher than the value of 8.3 ± 0.1% obtained for the ethylcellulose NPs.

scholarly journals Alginate-Mediated Synthesis of Hetero-Shaped Silver Nanoparticles and their Hydrogen Peroxide Sensing ability

2020 ◽  
Author(s):  
Sneha Bhagyaraj ◽  
Igor Krupa
Keyword(s):  
Hydrogen Peroxide ◽  
Particle Size ◽  
Silver Nanoparticles ◽  
Environmentally Benign ◽  
X Ray Diffraction ◽  
Ag Nps ◽  
X Ray ◽  
Transmission Electron ◽  
Wide Range ◽  
Pollution Detection

Silver nanoparticles have been the focus of extensive research for many decades due to their unique physical, chemical and electrical properties. Introducing new environmentally benign methods for the synthesis of silver nanoparticles is of great interest in the research community. In this work we propose a new method for the simple synthesis of stable heterostructured biopolymer (sodium alginate)-capped silver nanoparticles (Ag-NPs) based on green chemistry.The as-prepared nanoparticles were characterized using the ultraviolet–visible (UV–Vis) absorption spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and Dynamic light scattering (DLS) techniques. The results showed that the as-prepared Ag-NPs have a heterostructured morphology with particle size in the range 30 ± 18 – 60 ± 25 nm, showing a zeta potential of -62 mV. The silver nanoparticle formation was confirmed from UV-Vis spectra showing 424 nm as maximum absorption. The particle size and crystallinity of the as- synthesized nanoparticles were analyzed using TEM and XRD measurements respectively. FTIR spectra confirmed the presence of alginate as capping agent to stabilize the nanoparticles. The Ag-NPs also showed excellent sensing capability, with a linear response to hydrogen peroxide spanning a wide range of concentrations from 10-1 – 10-7 M, which indicates their high potential for water treatment applications, such as pollution detection and nanofiltration composites.

scholarly journals Bioremoval of PVP coated silver nanoparticles using Aspergillus niger: the role of exopolysaccharides

2021 ◽  
Author(s):  
Ola M. Gomaa ◽  
Amar Alrshim ◽  
Anindya Chanda
Keyword(s):  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Wastewater Treatment Plants ◽  
Aqueous Media ◽  
Visible Spectrum ◽  
Ag Nps ◽  
Characteristic Peak ◽  
Uv Visible

Abstract The present work aims to study the removal of Polyvinylpyrrolidone coated silver nanoparticles (PVP-Ag-NPs) using Aspergillus niger and depict the role of exopolysaccharides in the removal process. Our results show that the majority of PVP-Ag-NPs were attached to fungal pellets. About 74% and 88% PVP-Ag-NPs were removed when incubated with A. niger pellets and exopolysaccharide-induced A. niger pellets, respectively. Ionized Ag decreased by 553 and 1290 fold under the same conditions as compared to stock PVP-Ag-NP. PVP-Ag-PVP resulted in an increase in reactive oxygen species (ROS) in 24h. The UV-Visible spectrum shows the disappearance of Ag characteristic peak and the broadness of the spectrum suggested an increase in size. Dynamic Light Scattering results showed an increase in PVP-Ag-NPs size from 28.4 nm to 115.9 nm for A. niger pellets and 160.3 nm after removal by stress-induced A. niger pellets and further increased to 650.1 nm for in vitro EPS removal. Our findings show that EPS can be used for nanoparticle removal, by increasing the net size of nanoparticles in aqueous media, this will, in turn, facilitate its filtration through conventional filtration techniques commonly used at wastewater treatment plants.

scholarly journals Biosynthesis of silver nanoparticles from Pseudomonas fluorescens and their antifungal activity against Aspergillus niger and Fusarium udum

2021 ◽  
Author(s):  
Aneesh Sikka ◽  
Triveni Sodimalla ◽  
YALAVARTHI NAGARAJU
Keyword(s):  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Pseudomonas Fluorescens ◽  
Pathogenic Bacteria ◽  
Environmental Safety ◽  
Plant Diseases ◽  
Production Costs ◽  
Minimal Risk ◽  
Fusarium Udum ◽  
Vis Spectroscopy

Abstract In recent years, emerging plant diseases have posed a significant danger to global economies. Fungicide resistance and climate change are the two primary sources of pathogen outbreaks. The silver nanoparticles have been demonstrated to restrain pathogenic bacteria, fungi, and viruses with minimal risk of developing resistance and phytotoxicity. The synthesis of silver nanoparticles via chemical and physical methods raises concerns about environmental safety and production costs. The microbial bio-fabrication of silver nanoparticles is simple, cost-effective, and ready-to-use technology. In the current investigation, we used Pseudomonas fluorescens cell filtrates for the synthesis of silver nanoparticles. The silver nanoparticles were characterized using Dynamic Light Scattering (DLS), zeta potential (ζ), UV-Vis spectroscopy, SPR analysis, Transmission Electron Microscopy. Both silver nanoparticles and AgNO3 were tested for their ability to inhibit two phytopathogenic fungi, i.e., Fusarium udum and Aspergillus niger. Potent inhibition of mycelial growth was observed when silver nanoparticles were used at a concentration of 150 ppm. However, further research is needed to assess the toxicity of AgNPs before they are mass-produced and used in agricultural applications.

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