scholarly journals The predictor for Preoperative Coronal Imbalance in patients with Degenerative Lumbar Scoliosis--A related analysis on spinopelvic parameters

2021 ◽  
Author(s):  
Zifang ZHANG ◽  
Dengbin QI ◽  
Tianhao WANG ◽  
Zheng WANG ◽  
Yan WANG
Keyword(s):  
Regression Analysis ◽  
Cobb Angle ◽  
Pearson Correlation ◽  
Degenerative Lumbar Scoliosis ◽  
Spinopelvic Parameters ◽  
Major Curve ◽  
Coronal Balance ◽  
Coronal Imbalance ◽  
Group A ◽  
Group B

Abstract Background: The mechanism of coronal imbalance is still unclear. The relationships among CBD and major curve, L4 and L5 tilt have been reported, which were not explicit although. The aim of this retrospective study was to explore and examine the association between spinopelvic parameters and coronal balance distance (CBD) in degenerative lumbar scoliosis (DLS) patients.Methods Following institutional ethics approval, the radiographs of 161 DLS patients were retrospectively reviewed. The coronal measurements involving CBD, L4 coronal tilt, L5 coronal tilt, fractional curve, pelvic coronal tilt, the Cobb angle and included vertebras of major curve were documented. All of those participants were divided into group A (CBD≥30mm) and group B (CBD < 30mm). Statistical analysis was performed to compare the difference of continuous and categorical variables. Pearson-Correlation, and Multiple-Regression Analysis were performed to explore the related parameter with CBD.Results The CBD in 31 (19.25%) DLS patients at pre-operation was over 30mm. With similar major Cobb angle, main curve of patients in the group A had less involving vertebras (P=0.009), but more rotation of the apical vertebra (P<0.001) compared with that in the group B respectively. Additionally, the L4 coronal tilt in group A was much larger (P=0.02). Pearson-correlation analysis showed that CBD had significant relationship with L4/L5 coronal tilt(P<0.001), weak relationship with fractional curve (P=0.027), but linearly independent with pelvic tilt and major Cobb. Stepwise Multiple- regression analysis revealed that only L4 tilt was the independent predictor for major Cobb and CBD.Conclusions The prevalence of coronal imbalance at pre-operation in DLS patients is about 20%. With similar major Cobb, the less the number of involving vertebra, the higher the incidence of coronal imbalance in DLS patients. L4 coronal tilt would be the predictive variable for progression of scoliosis and global coronal malalignment. Pelvis, the foundation of spine, had less effect on full-spine coronal balance.

scholarly journals A Method for Quantifying Cervical Sagittal Morphology Using the Cervical Sagittal Triangle Area Coefficient

2021 ◽  
Author(s):  
Yingjun Guo ◽  
Han Wang ◽  
Hao Liu ◽  
Beiyu Wang ◽  
Chen Ding ◽  
...  
Keyword(s):  
Cobb Angle ◽  
Clinical Symptoms ◽  
Pearson Correlation ◽  
Diagnostic Value ◽  
Advantages And Disadvantages ◽  
Group A ◽  
Group B ◽  
Flexion And Extension ◽  
Selection Of ◽  
Good Agreement

Abstract Background: A series of methods, which use angle to quantitatively evaluate cervical sagittal morphology cannot objectively reflect the advantages and disadvantages in some cases. In this study, we try to use the area methods to supplement it in above cases. Methods: The Cobb angle, range of motion (ROM) of C2-7, and antero-posterior diameter of atlas (C1-APD) were measured at neutral, flexion, and extension X-ray radiographs of the cervical spine in all 191 patients. Patients were divided into Group A and Group B according to whether their cervical sagittal morphology can be objectively quantified with Cobb angle. Pearson correlation analysis was used to compare the consistency of the results, paired t-test was used to compare the dispersion coefficient, receiver operator characteristic (ROC) curve was used to evaluate the diagnostic value.Results: The C5 vertebra was used as the vertex to construct the cervical sagittal triangle, and according to the different selection of the other two points, the triangle was further divided into four types. The Area Coefficient (AC) was defined as "B". In Group A, whose cervical sagittal morphology could be objectively quantified with Cobb angle, the AC showed greatly consistency, while in Group B, it was less consistent. AC results have stronger predictive value for clinical symptoms in Group B. The dispersion coefficients of the results measured by the four area methods are significantly smaller than the Cobb angle. The results of quantifying cervical ROM by area methods were also in good agreement with Cobb angle. Conclusion: Compared with Cobb angle, AC has better objectivity, stability, and clinical significance.

scholarly journals A Method for Quantifying Cervical Sagittal Morphology Using the Cervical Sagittal Triangle Area Coefficient

2021 ◽  
Author(s):  
Yingjun Guo ◽  
Han Wang ◽  
Hao Liu ◽  
Beiyu Wang ◽  
Chen Ding ◽  
...  
Keyword(s):  
Cobb Angle ◽  
Clinical Symptoms ◽  
Pearson Correlation ◽  
Advantages And Disadvantages ◽  
Group A ◽  
Pearson Correlation Analysis ◽  
Group B ◽  
Flexion And Extension ◽  
Selection Of ◽  
Good Agreement

Abstract BackgroundA series of methods, which use angle to quantitatively evaluate cervical sagittal morphology cannot objectively reflect the advantages and disadvantages in some cases. In this study, we try to use the area methods to supplement it in above cases.MethodsThe Cobb angle, range of motion (ROM) of C2-7, and antero-posterior diameter of atlas (C1-APD) were measured at neutral, flexion, and extension X-ray radiographs of the cervical spine in all 191 patients. Patients were divided into Group A and Group B according to whether their cervical sagittal morphology can be objectively quantified with Cobb angle. Pearson correlation analysis was used to compare the consistency of the results, paired t-test was used to compare the dispersion coefficient, receiver operator characteristic (ROC) curve was used to evaluate the diagnostic value.ResultsThe C5 vertebra was used as the vertex to construct the cervical sagittal triangle, and according to the different selection of the other two points, the triangle was further divided into four types. The Area Coefficient (AC) was defined as "\(\text{A}\text{C}=\frac{\left(\text{A}\text{r}\text{e}\text{a} \text{o}\text{f} \text{s}\text{a}\text{g}\text{i}\text{t}\text{t}\text{a}\text{l} \text{t}\text{r}\text{i}\text{a}\text{n}\text{g}\text{l}\text{e}\right)}{{\text{C}1-\text{A}\text{P}\text{D}}^{2}}\)". In Group A, whose cervical sagittal morphology could be objectively quantified with Cobb angle, the AC showed greatly consistency, while in Group B, it was less consistent. AC results have stronger predictive value for clinical symptoms in Group B. The dispersion coefficients of the results measured by the four area methods are significantly smaller than the Cobb angle. The results of quantifying cervical ROM by area methods were also in good agreement with Cobb angle.ConclusionCompared with Cobb angle, AC has better objectivity, stability, and clinical significance.

Bioinformatics Study on Serum Triglyceride Levels for Analysis of a Potential Risk Factor Affecting Blood Pressure Variability

2019 ◽  
Vol 14 (5) ◽  
pp. 376-385 ◽  
Author(s):  
Lin Xu ◽  
Jiangming Huang ◽  
Zhe Zhang ◽  
Jian Qiu ◽  
Yan Guo ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Systolic Blood Pressure ◽  
Blood Pressure Variability ◽  
Partial Correlation ◽  
Pearson Correlation ◽  
Target Organ Damage ◽  
Serum Triglyceride ◽  
Hypertensive Patients ◽  
Group A ◽  
Group B

Objective: The purpose of this study was to establish whether Triglycerides (TGs) are related to Blood Pressure (BP) variability and whether controlling TG levels leads to better BP variability management and prevents Cardiovascular Disease (CVD). Methods: In this study, we enrolled 106 hypertensive patients and 80 non-hypertensive patients. Pearson correlation and partial correlation analyses were used to define the relationships between TG levels and BP variability in all subjects. Patients with hypertension were divided into two subgroups according to TG level: Group A (TG<1.7 mmol/L) and Group B (TG>=1.7 mmol/L). The heterogeneity between the two subgroups was compared using t tests and covariance analysis. Results: TG levels and BP variability were significantly different between the hypertensive and non-hypertensive patients. Two-tailed Pearson correlation tests showed that TG levels are positively associated with many BP variability measures in all subjects. After reducing other confounding factors, the partial correlation analysis revealed that TG levels are still related to the Standard Deviation (SD), Coefficient of Variation (CV) of nighttime systolic blood pressure and CV of nighttime diastolic blood pressure, respectively (each p<0.05). In the subgroups, group A had a lower SD of nighttime Systolic Blood Pressure (SBP_night_SD; 11.39±3.80 and 13.39±4.16, p=0.011), CV of nighttime systolic blood pressure (SBP_night_CV; 0.09±0.03 and 0.11±0.03, p=0.014) and average real variability of nighttime systolic blood pressure (SBP_night_ARV; 10.99±3.98 and 12.6±3.95, p=0.024) compared with group B, even after adjusting for age and other lipid indicators. Conclusion: TG levels are significantly associated with BP variability and hypertriglyceridemia, which affects blood pressure variability before causing target organ damage.

Assessment of the Correlation Between Insertion Torque and Resonance Frequency Analysis of Implants Placed in Bone Tissue of Different Densities

2014 ◽  
Vol 40 (3) ◽  
pp. 259-262 ◽  
Author(s):  
Luiz Carlos Magno Filho ◽  
Fabiano Ribeiro Cirano ◽  
Fernando Hayashi ◽  
Hsu Shao Feng ◽  
Alexandre Conte ◽  
...  
Keyword(s):  
Resonance Frequency ◽  
Dental Implants ◽  
Frequency Analysis ◽  
Pearson Correlation ◽  
T Test ◽  
Insertion Torque ◽  
Type I ◽  
Resonance Frequency Analysis ◽  
Group A ◽  
Group B

The primary stability of dental implants is fundamental for osseointegration. Therefore, this study aimed to assess the correlation between insertion torque (IT) and resonance frequency analysis (RFA) of implants placed in mandibles and maxillas of different bone densities. Eighty dental implants were placed in maxillas and mandibles, and IT and the implant stability quotient (ISQ) were measured at the time of implant insertion. Bone density was assessed subjectively by the Lekholm and Zarb index. The type I and II densities were grouped together (group A)as were the type III and IV densities (group B). The IT in group A was higher (Student t test, P = .0013) than in group B (46.27 ± 18.51 Ncm, 33.62 ± 14.74 Ncm, respectively). The implants placed in group A showed higher ISQ (Student t test, P = .0004) than those placed in group B (70.09 ± 7.50, 63.66 ± 8.00, respectively). A significant correlation between IT and the ISQ value was observed for group A (Pearson correlation test; r = 0.35; P = .0213) and for group B (r = 0.37; P = .0224). Within the limitations of this study, it was possible to conclude that there is a correlation between IT and RFA of implants placed in mandibles and maxillas of different bone densities.

scholarly journals Multiple Factor Analysis of Symptomatic Intracranial Arterial Stenosis

2017 ◽  
Vol 3 (2) ◽  
pp. 84-89
Author(s):  
Chuanqiang Qu ◽  
Yuanyuan Zhao ◽  
Qinjian Sun ◽  
Yifeng Du
Keyword(s):  
Risk Factors ◽  
Coronary Heart Disease ◽  
Logistic Regression ◽  
Regression Analysis ◽  
Risk Factor ◽  
Protective Factor ◽  
Arterial Stenosis ◽  
Intracranial Arterial Stenosis ◽  
Group A ◽  
Group B

Objective To discuss the correlation among intracranial arterial stenosis and its risk factors. Methods A total of 486 patients with transient ischemic attack (TIA) or ischemic cerebral infarction were examined using color doppler flow imaging (CDFI) and transcranial doppler ultrosonography (TCD). According to the degrees of extracranial arterial stenosis, patients with mild-to-moderate extracranial stenosis were classified into group A (435 cases) while those with constant severe stenosis or occlusion were classified into group B (51 cases). The differences between the two groups of risk factors were compared, and the multi-factor logistic regression analysis of risk factors associated with moderately severe intracranial arterial stenosis was performed. Results ① The risk factors that were significantly associated with intracranial arterial stenosis included age (P = 0.034) and gender (P = 0.044). ② Intracranial artery stenosis was observed in both anterior and posterior cerebral arteries in patients with hypertension, diabetes, and coronary heart disease respectively (P < 0.05). ③ Compared with group A, patients in group B were older (P = 0.000), with a higher proportion of men (P = 0.037), and the intracranial arterial stenosis degrees were significantly higher (P = 0.013). ④ Multi-factor logistic regression analysis showed that diabetes is a risk factor for moderately severe intracranial arterial stenosis (P < 0.05), and hyperlipidemia is a protective factor (P = 0.012). Conclusions Age, gender, hypertension, diabetes, coronary heart disease, and smoking are risk factors for the distribution of intracranial arterial stenosis. The degrees of intracranial arterial stenosis are related with extracranial arterial stenosis. Diabetes is a risk factor for moderately severe intracranial arterial stenosis while hyperlipidemia is a protective factor.

scholarly journals P1166DOES PERITONEAL PROTEIN LOSS AFFECT CARDIOVASCULAR MORTALITY OF PERITONEAL DIALYSIS PATIENTS ?

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Marios Theodoridis ◽  
Stylianos Panagoutsos ◽  
Ioannis Neofytou ◽  
Konstantia Kantartzi ◽  
Efthimia Mourvati ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Peritoneal Dialysis ◽  
Cardiovascular Mortality ◽  
Cox Regression ◽  
Dialysis Patients ◽  
Protein Loss ◽  
Cox Regression Analysis ◽  
Kaplan Meier ◽  
Group A ◽  
Group B

Abstract Background and Aims Peritoneal protein loss (PPL) through peritoneal effluent has been a well-recognized detrimental result of peritoneal dialysis (PD). The amount of protein lost will depend on dialysis time, protein size, its serum concentration and other factors including patients’ clinical status. Peritoneal protein loss may be a manifestation of endothelial dysfunction, as with another type of capillary protein leakage, microalbuminuria, a recognized endothelial dysfunction marker. The aim of this study was to retrospectively evaluate the influence of PPL on cardiovascular mortality of peritoneal dialysis patients Method This is a single center retrospective study of 84 PD patients (m=54, f=30) with mean age of 65.2±17 years, mean PD duration of 43.2±24.9 months conducted for the time period from 2006 to 2019 (13 years). The patients were divided into two groups according to the amount of protein excreted during the modified Peritoneal Equilibration Test (PET) procedure using PD solution of 3.86% DW, 2 Lt infusion volume for total time of 4 hours. The total amount of proteins excreted was calculate from PET by multiplying the concentration of proteins at the end of the test with the total volume of PD fluid at the same time. Group A excreted a total amount of proteins &lt; 1.55 gr (median value) at the end of PET test and Group B &gt; 1.55 gr. The cumulative all-cause and cardiovascular survival of the PD patients was calculated by Kaplan Meier while the possible effect of any parameter in survival rates was evaluated by using Cox Regression analysis Results There was not any statistically significant difference between the two groups according to PD duration, age, dialysis adequacy targets, Residual Renal Function(RRF), BMI, ultrafiltration volume during PET and their transport status. The cumulative all-cause survival using Kaplan-Meier analysis revealed no statistically significant deference between the two groups (Log Rank p=0.55) even though mortality risk was adjusted for several factors (Cox Regression). When cardiovascular survival, using Cox Regression analysis, was adjusted for age, sex, Diabetes, PD modality, dialysis Kt/V and RRF we found that Group A (with protein excretion &lt; 1.55 gr) had statistically significant better cardiovascular survival (p=0.029) compared to Group B. We confirm these results while trying to find among the total of our patients the possible risk factors for cardiovascular mortality. Using Cox Regression analysis, the amount of protein excreted during PET procedure and the type of PD solutions (high or low in GDPs) used were statistically significant (p=0.019 and p=0.04 respectively) independent risk factors for cardiovascular survival in our patients. Conclusion These results indicate that protein loss during peritoneal dialysis procedure has negative impact on cardiovascular mortality and survival of PD patients. Additionally, the use of PD solutions with low Glucose Degradation Products (GDPs) and AGEs may improve PD patient’s cardiovascular survival. Randomized interventional studies are encouraged to address the pathological concern of PPL in the future, namely its effects on cardiovascular conditions or its role as marker and effort to reduce PPL using ACE inhibitors or vit D should be considered only if it diminishes cardiovascular morbidity or mortality.

A randomized study of urgent computed tomography–based hematoma puncture and aspiration in the emergency department and subsequent evacuation using craniectomy versus craniectomy only

2012 ◽  
Vol 117 (3) ◽  
pp. 566-573 ◽  
Author(s):  
Bo Xiao ◽  
Fang-Fang Wu ◽  
Hong Zhang ◽  
Yan-Bin Ma
Keyword(s):  
Emergency Department ◽  
Logistic Regression ◽  
Regression Analysis ◽  
Scale Score ◽  
Logistic Regression Analysis ◽  
Randomized Study ◽  
Surgical Decompression ◽  
Pupil Response ◽  
Group A ◽  
Group B

Object When treating patients with a spontaneous supratentorial massive (≥ 70 ml) intracerebral hemorrhage (ICH), the results of surgery are gloomy. A worsening pupil response has been observed in patients preoperatively, despite blood pressure control and diuretic administration. Because open surgery needs time for decompression to occur, the authors conducted a prospective randomized study to determine whether patients who have suffered a massive ICH can benefit from a more urgently performed decompressive procedure. Methods Overall, 36 eligible patients admitted 6 or fewer hours post-ictus were enrolled in the study. In Group A, 12 patients underwent CT-based hematoma puncture and partial aspiration in the emergency department (ED) and subsequent evacuation via a craniectomy; in Group B, 24 patients underwent hematoma evacuation via a craniectomy only. Pupil responses were categorized into 5 grades (Grade 0, bilaterally fixed; Grade 1, unilaterally fixed with the fixed pupil > 7 mm; Grade 2, unilaterally fixed with the fixed pupil ≤ 7 mm; Grade 3, a unilaterally sluggish response; and Grade 4, a bilaterally brisk response). Grades were obtained on admission, at surgical decompression (defined as the point at which liquid hematoma began to flow out in Group A and at dural opening in Group B), and at completion of craniectomy. The Barthel Scale was used to assess survivors' functional outcome at 12 months. Comparisons were made between Groups A and B. Logistic regression analysis was used to evaluate the positive likelihood ratio of all variables for survival and function (Barthel Scale score of ≥ 35 at 12 months). Results Decompressive surgery was undertaken approximately 60 minutes earlier in Group A than B. A worsening pupil reflex before decompression was observed in no Group A patient and in 9 Group B patients. At the time of decompression pupil response was better in Group A than B (p < 0.05). Although only approximately one-third of the hematoma volume documented on initial CT scanning had been drained before the craniectomy in Group A, when partial aspiration was followed by craniectomy, better pupil-response results were obtained in Group A at the completion of craniectomy, and survival rate and 12-month Barthel Scale score were better as well (p < 0.05). Logistic regression analysis revealed that one variable, a minimum pupil grade of 3 at the time of decompression, had the highest predictive value for survival at 12 months (8.0, 95% CI 2.0–32.0), and a pupil grade of 4 at the same time was the most valuable predictor of a Barthel Scale score of 35 or greater at 12 months (15.0, 95% CI 1.9–120.9). Conclusions Patients with massive spontaneous supratentorial ICHs may benefit from more urgent surgical decompression. The results of logistic regression analysis implied that, to improve long-term functional outcome, decompression should be performed in patients before herniation occurs. Due to the fact that most of these patients have signs of herniation when presenting to the ED and because conventional surgical decompression requires time to take effect, this combination of surgical treatment provides a feasible and effective surgical option.

scholarly journals Increasing dominant follicular proportion negatively associated with good clinical outcomes in GnRH-a prolonged protocol: a large-sample retrospective analysis

2021 ◽  
Author(s):  
Houming Su ◽  
Youhua Lai ◽  
Jie Li ◽  
Tingting Liao ◽  
Licheng Ji ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
Retrospective Analysis ◽  
Formation Rate ◽  
Clinical Pregnancy ◽  
Blastocyst Formation ◽  
Negatively Associated ◽  
Large Sample ◽  
Group A ◽  
Group B

Abstract Background: Nowadays, there is no universal criteria for trigger time during controlled ovarian hyperstimulation (COH). Particularly, in the so-called GnRH-a prolonged protocol, widely used in China, the ideal time to trigger ovulation is not yet well defined. Methods: This was a large-sample retrospective analysis. Between January 2016 and January 2020, 1,925 young patients who underwent their first in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles with normal ovarian response were divided into three groups based on their dominant follicular proportions (DFP, defined as ≥18 mm follicles /≥14 mm follicles; Group A: < 30%; Group B: 30% - 60%; Group C: ≥ 60%). Binary logistic regression and multivariate linear regression were used to assessed whether DFP levels were related to clinical pregnancy, number of blastocysts frozen, blastocyst formation rate and low blastocysts frozen. Results: Binary logistics regression analysis showed that compared with Group A, the OR for clinical pregnancy was 1.345 in Group B (P = 0.023); however, there was no statistical difference between Group C and Group A (P = 0.216). On one hand, multivariate linear regression analysis indicated that DFP was negatively associated with number of blastocysts frozen (β ± SE: Group B vs Group A = -0.319 ± 0.115, P = 0.006; Group C vs Group A = -0.432 ± 0.154, P = 0.005) as well as blastocyst formation rate (β ± SE: Group B vs Group A = -0.035 ± 0.016, P = 0.031; Group C vs Group A = -0.039 ± 0.021, P = 0.067). On the other hand, compared with group A, the OR for low blastocyst frozen was 1.312 in Group B (P = 0.039) and was 1.417 in Group C (P = 0.041). Conclusions: Excessive delay of trigger in GnRH-a prolonged protocol might reduce the developmental potential of oocytes and reduce the number of available blastocysts, which might result in a lower cumulative pregnancy rate. But further confirmation by strict prospective randomized controlled study should be needed.Trial registration: https://clinicaltrials.gov/; NCT03305510; Registered 08 October 2017 - Retrospectively registered.

P5456The role of ST2 as an early marker of endothelial dysfunction in hypertensive patients

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
A D Farcas ◽  
M A Stoia ◽  
M Mocan ◽  
R M Chiorescu ◽  
D L Mocan-Hognogi ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Endothelial Dysfunction ◽  
Diastolic Dysfunction ◽  
Brachial Artery ◽  
Predictive Marker ◽  
Relative Increase ◽  
Left Ventricular ◽  
Hypertensive Patients ◽  
Group A ◽  
Group B

Abstract Background The association between endotelial dysfunction and progression of diastolic dysfunction suggests that endotelial and myocardial impairment might have similar pathogenic pathways. Moreover, in hypertensive diabetics the endotelial function is worse in those with impaired diastolic performance compared to normal diastolic performance. ST2 provides additional data to echocardiography in early detection of diastolic performance alteration in hypertension (HT) and is a prominent predictive marker of worse clinical outcome in hypertensive patients. There is scarce data on ST2 role in detection of early vascular involvement in hypertensive patients. Purpose The aim of the study is to evaluate whether ST2 levels correlate with impaired endotelial function in hypertensive patients. Methods Our prospective study included 84 hypertensive patients (mean age 52.9±14.4 yrs, 54.3% males and 45.7% females), after they signed an informed consent. All patients underwent clinical and laboratory (including ST2) evaluation. To investigate endothelial function, we performed ultrasound measurement of the brachial artery diameter both at rest and during reactive hyperemia in the muscle distal to the brachial artery (BA) which causes endothelium-dependent vasodilatation. The study methodology was approved by the Ethical Committee and statistical data processing was performed with SPSS. Results Patients were assigned to group A – those without left ventricular hypertrophy (LVH) and group B – those with LVH. There were no significant differences regarding gender, age and HT stage between groups A and B. Patients in group A had lower ST2 levels [18.83 (13.98 - 42.05) vs 55.63 (48.6 - 100.21) vs, p<0.001] and fewer risk factors (1.4 vs 2.6, p<0.01) compared to group B. We found significant differences regarding diastolic performance parameters between the groups. The relative increase in diameter (% FMD; DD = D 100) was significantly higher in group A than group B (6.67±0.43% vs 3.10±0.33%, p<0.01). ST2 levels were greater in group B and correlated with the degree of endotelial dysfunction evaluated by flow-mediated dilatation (FMD) (r=0.411, p<0.01). Multiple regression analysis showed that ST2 had a significant negative correlation with the relative increase in arterial diameter while multivariate Cox regression analysis showed ST2 was an independent predictor of endothelial dysfunction severity in hypertensive patients (hazard ratio: 4.012, 95% confidence interval: 1.207–31.24, P=0.031). Conclusions ST2 levels correlate with cardiovascular risk and are a significant predictive marker of endothelial dysfunction in hypertensive patients regardless of diastolic dysfunction.

scholarly journals Catalase and Glutathione Peroxidase in Dogs Naturally Infected by Leishmania infantum

2018 ◽  
Vol 44 (1) ◽  
pp. 6 ◽  
Author(s):  
Belarmino Eugênio Lopes-Neto ◽  
Glauco Jonas Lemos Santos ◽  
Adam Leal Lima ◽  
Maritza Cavalcante Barbosa ◽  
Talya Ellen Jesus dos Santos ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Pearson Correlation ◽  
Clinical Signs ◽  
Erythrocyte Count ◽  
Control Group ◽  
Positive Correlation ◽  
Antioxidant Agents ◽  
Group A ◽  
Group B ◽  
Gpx Activity

Background: Canine leishmaniasis (CanL) is caused by an obligatory intracellular parasite of Leishmania genus that  affects organs and tissues. Several studies evaluate the role of reactive oxygen species (ROS) in the pathogenesis of many diseases. The overproduction of ROS on infectious diseases can induce an imbalance between oxidants and antioxidants at cellular or systemic level. Thus, the aim of this study was to evaluate the activity of antioxidant enzymes in CanL.Materials, Methods & Results: Females (n = 17) and males (n = 10), at different ages and with different weight, were selected for this study. Dogs were divided into two groups according classical clinical signs and sorological test to CanL. Animals were considered infected based on indirect immunofluorescent assay and ELISA titration ≥ 1:40.  Group B (n = 15) composed by positive dogs to CanL from Zoonosis Control Center of Fortaleza (Ceará, Brazil) and group A (n = 12) was composed by dogs from private kennel that were serologically negative to L. infantum and had absence of clinical signs to CanL. Blood sample were collected for evaluation of hematological and biochemical parameters and glutathione peroxidase (GPx) and catalase (CAT) enzymatic activity. Data were analyzed by Student’s t-test and Pearson correlation coefficient (P < 0.05). Total proteins (TP, mg/dL) and alkaline phosphatase (ALP, U/L) were increased (P < 0.05) on group B (8.2 ± 1.2; 165.4 ± 46.4) when compared to group A (6.5 ± 1.1; 109.1 ± 38.3), respectively. Hemoglobin (Hb; g/dL) and hematocrit (Hct; %) were decreased (P < 0.05) on Group B (14.7 ± 1.8; 48.2 ± 5.7) when compared to group A (16.5 ± 1.3; 52.1 ± 2.4), respectively. Group B presented CAT (U/g Hb) and GPx (mU/mg Hb) lower (189.4 ± 90.4; 3,609.6 ± 1,569.1) than group A (326.6 ± 104.5; 5,055.6 ±1,569.1), respectively (P < 0.001). Positive correlation was observed between RBC and CAT; however, it was not significant.Discussion: Organisms require a good defense system in order to revert the overproduction of free radicals and consequently the injuries caused by them. This is possible through the production of antioxidant agents, which act on oxidative prevention and on tissue and cellular regeneration, by taking the reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzymes in the frontline. Erythrocyte changes promoted by CanL suggest possible correlation between anemia and the appearance of clinical signs, which in many cases is not seen. Erythrocytes contain SOD, CAT and GPx enzymes, thus, changes in these cells will reflect on the activity of these enzymes. In our results only CAT showed positive correlation with erythrocyte count, however it was not significant. GPx activity was lower (P < 0.001) in infected dogs than control group. This result agrees with another study, which showed a decrease in GPx levels in CanL, although it was not significant. However, it was found a positive correlation (P < 0.001) between erythrocytes and GPx activity and between hemoglobin and GPx activity in animals with leishmaniasis. These results suggest that the reduction in detoxification activity can be related to the decrease in erythrocyte count and that the GPx activity depends on the control mechanism of the antioxidant system in CanL. Furthermore, this result could be associated with decrease of blood cell count in animals with CanL, once GPx is an erythrocyte enzyme, which plays an important role in hemoglobin protection against oxidative damage. This study was carried out in naturally infected dogs with L. infantum. In conclusion, CAT and GPx activities are relate to oxidative stress induced by L. infantum infection and can be used as biomarkers on CanL.

Sign in / Sign up

Export Citation Format

Share Document