Zinc Oxide Nanoparticles Improve Testicular Steroidogenesis Machinery Dysfunction In Benzo [α] Pyrene Challenged Rats

Abstract Although Zinc oxide nanoparticles (ZnO NPs) in low doses have potentially positive effects on reproduction by their antioxidant effects, the defensive role of Zinc nanomaterials against environmental pollutants that affect male reproduction has not been adequately studied. We designed our study to assess the impact of ZnO NPs towards reproductive dysfunction induced by Benzo[α]Pyrene (B[a]P). Forty-eight mature male rats were randomly distributed into six equal groups: G1; negative control, G2&3- positive control I &II (either 10 or 30 mg ZnO NPs / kg BW); G4. (150 mg Bap / kg BW), G 5 & 6 (Co- administrated B[a]P with different concentrations of ZnO NPs). Oxidative stress biomarkers, semiquantitative real-time PCR for steroidogenic enzymes (CY11A1, StAR, and 3β- HSD), testosterone levels and histopathology in the liver, kidney, and testicles were examined for this investigation. B[a] P treated group showed significant deterioration in all reproductive parameters and induced oxidative stress. Co-administration ZnO NPs eased oxidative stress and effectively increased the expression of CY11A1, StAR, and 3β- HSD and improved histopathological changes in the examined organs. Our results using the selected doses and with Nano particle properties confirm that ZnO NPs have an obvious ameliorative effect against B[a] P.

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Zinc oxide nanoparticles improve testicular steroidogenesis machinery dysfunction in benzo[α]pyrene-challenged rats

Scientific Reports ◽

10.1038/s41598-021-91226-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Niveen M. Daoud ◽

Mohamed S. Aly ◽

Omaima H. Ezzo ◽

Naglaa A. Ali

Keyword(s):

Oxidative Stress ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Molecular Mechanisms ◽

Regulatory Protein ◽

Male Rats ◽

Oxide Nanoparticles ◽

Mrna Levels ◽

Zno Nps ◽

Positive Effects

AbstractZinc oxide nanoparticles (ZnO NPs) demonstrate potential positive effects on reproduction. However, their protective role against the reproductive toxicity pollutants has not yet been adequately studied at the molecular level. This study was designed to assess this objective using Benzo[α]pyrene B[a]P as reproductive toxic agent . Forty-eight mature male rats were randomly distributed into six groups: Group1 (negative control); Groups 2 and 3 (positive control I and II, wherein the animals were treated with 10 and 30 mg ZnO NPs/kg BW, respectively); Group 4 (B[a]P group; treated with 150 mg B[a]P/kg BW); and Groups 5 and 6 (subjected to B[a]P treatment co-administered with different concentrations of ZnO NPs). We investigated oxidative stress biomarkers; cholesterol side-chain cleavage enzyme (CYP11A1), steroidogenic acute regulatory protein (StAR), and 3β-hydroxysteroid dehydrogenase (3β-HSD) gene expression; testosterone levels; and histopathology of the liver, kidney, and testicles. The B[a]P-treated group showed significant deterioration in all reproductive parameters and displayed induced oxidative stress. ZnO NPs remarkably reduced oxidative stress, effectively upregulated the mRNA levels of CY11A1, StAR, and 3β-HSD, and improved the histological pictures in the examined organs. At their investigated doses and given their NPs properties, ZnO NPs demonstrated a marked ameliorative effect against the reproductive toxic effects of B[a]P. Further studies are needed to thoroughly investigate the molecular mechanisms of ZnO NPs.

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The Effects of Pentoxifylline on Memory in Male Rats Treated with Zinc Oxide NPs

The Journal of Qazvin University of Medical Sciences ◽

10.32598/jqums.25.1.1 ◽

2021 ◽

Vol 25 (1) ◽

pp. 1-10

Author(s):

Niloufar Darbandi ◽

◽

Zeynab Vasheghani Farahani ◽

Hamidreza Momeni ◽

◽

...

Keyword(s):

Oxidative Stress ◽

Zinc Oxide ◽

Treatment Group ◽

Blood Serum ◽

Zinc Oxide Nanoparticles ◽

Male Rats ◽

Control Group ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Ca1 Region

Background: Zinc oxide Nanoparticles (NPs) present irreversible effects on the nervous system, memory, and learning. Objective: The current study aimed to investigate the effects of pentoxifylline on memory impairments, CA1 hippocampal pyramidal cells, and blood serum antioxidant enzymes in male rats treated with zinc oxide NPs. Methods: Male Wistar rats were divided into the control, zinc oxide NPs (1.25 mg/kg), pentoxifylline (50 mg/kg), and pentoxifylline with zinc oxide NPs groups. In all study groups, saline, zinc oxide NPs, and pentoxifylline were intraperitoneally injected 30 minutes before training. In the co-treatment group, pentoxifylline was injected one hour before injecting Zno NPs. After performing the behavioral test, the tested animals’ brains were fixed and the number of healthy neurons in the CA1 region of the hippocampus was counted. In all research groups, malondialdehyde levels, total antioxidant power, superoxide dismutase levels, and glutathione peroxidase in blood serum were measured. Results: Zinc oxide nanoparticles decreased memory and the number of healthy neurons in the CA1 region of the hippocampus and increased oxidative stress in blood serum, compared to the controls. In the co-treatment group, using pentoxifylline improved the above-mentioned factors and reached the level of the control group. Pentoxifylline alone presented no significant effect on the aforementioned characteristics, compared to the control group. Conclusion: ZnO NPs may decrease memory retrieval and cause cell death in the pyramidal neurons of the CA1 region of the hippocampus by increasing oxidative stress. Pentoxifylline, as a potent antioxidant, can prevent the harmful effects of ZnO NPs.

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The Impact of Zinc Oxide Nanoparticles on Male (In)Fertility

Materials ◽

10.3390/ma13040849 ◽

2020 ◽

Vol 13 (4) ◽

pp. 849 ◽

Cited By ~ 5

Author(s):

Ana Rita Pinho ◽

Sandra Rebelo ◽

Maria de Lourdes Pereira

Keyword(s):

Zinc Oxide ◽

Reproductive System ◽

Male Fertility ◽

Zinc Oxide Nanoparticles ◽

Engineered Nanoparticles ◽

Male Reproductive System ◽

Oxide Nanoparticles ◽

Exceptional Set ◽

Zno Nps ◽

The Impact

Zinc oxide nanoparticles (ZnO NPs) are among nanoscale materials, attracting increasing attention owing to their exceptional set of characteristics, which makes these engineered nanoparticles a great option for improving the quality and effectiveness of diagnosis and treatment. The capacity of ZnO NPs to induce reactive oxygen species (ROS) production, DNA damage, and apoptosis represents a promise for their use in both cancer therapy and microbial treatment. However, their intrinsic toxicity together with their easy entrance and accumulation in organism have raised some concerns regarding the biomedical use of these NPs. Several studies have reported that ZnO NPs might induce cytotoxic effects on the male reproductive system, compromising male fertility. Despite some advances in this area, the knowledge of the effects of ZnO NPs on male fertility is still scarce. Overall, a brief outline of the major ZnO NPs biomedical applications and promises in terms of diagnostic and therapeutic use will also be explored. Further, this review intends to discuss the effect of ZnO NPs exposure on the male reproductive system and speculate their effects on male (in)fertility.

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UVB exposure enhanced the dermal penetration of zinc oxide nanoparticles and induced inflammatory responses through oxidative stress mediated by MAPKs and NF-κB signaling in SKH-1 hairless mouse skin

Toxicology Research ◽

10.1039/c6tx00026f ◽

2016 ◽

Vol 5 (4) ◽

pp. 1066-1077 ◽

Cited By ~ 9

Author(s):

Anu Pal ◽

Shamshad Alam ◽

Lalit K. S. Chauhan ◽

Prem N. Saxena ◽

Mahadeo Kumar ◽

...

Keyword(s):

Oxidative Stress ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Inflammatory Responses ◽

Mouse Skin ◽

Hairless Mouse ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Dermal Penetration ◽

Uvb Exposure

UVB exposure enhances the internalization of ZnO-NPs and caused changes in surface morphology of SKH-1 mouse skin.

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The Interaction of Zinc Oxide/Green Tea Extract Complex Nanoparticles and its Effect on Monosodium Glutamate Toxicity in Liver of Rats

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190408120532 ◽

2019 ◽

Vol 20 (6) ◽

pp. 465-475 ◽

Cited By ~ 5

Author(s):

Fawziah A. Al-Salmi ◽

Reham Z. Hamza ◽

Nahla S. El-Shenawy

Keyword(s):

Oxidative Stress ◽

Zinc Oxide ◽

Green Tea ◽

Zinc Oxide Nanoparticles ◽

Monosodium Glutamate ◽

Green Tea Extract ◽

Control Group ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are increasingly utilized in both industrial and medical applications. Therefore, the study was aimed to investigate the effect of green nanoparticle complex (green tea extract/zinc oxide nanoparticles complex, GTE/ZnO NPs) on oxidative stress induced by monosodium glutamate (MSG) on the liver of rats. Methods: Wistar male rats (n=64) weighing between 200-250 g were divided randomly into eight groups: control group was given physiological saline (1 mg/kg), two groups were treated with two different doses of MSG (MSG-LD, MSG-HD; 6 and 17.5 mg/Kg, respectively), GTE was given 1 mg/mL, 5th group was treated with ZnO NPs and 6th group was treated with GTE/ZnO NPs complex while, 7th and 8th groups were treated with MSG-LD + GTE/ZnO NPs complex and MSG-HD + GTE/ZnO NPs complex, respectively. All substances were given orally for 30 consecutive days. At the end of the study, the liver was homogenized for measurement of the oxidative stress status and anti-inflammatory biomarkers as well as histological and transmission alternations. Results: Results showed that the antioxidant enzymes activity and glutathione level were significantly decreased in MSG groups than control in a dose-dependent manner. Conversely, the malondialdehyde and inflammatory cytokines levels were significantly increased in MSG groups than the control group. The liver indicated no evidence of alteration in oxidative status, anti-inflammatory and morphological parameters in GTE, ZnO NPs and GTE/ZnO NPs complex groups. Conclusion: In conclusion, MSG at both doses caused oxidative stress and inflammation on liver after 28 days of exposure that supported histological analysis and transmission view of hepatic parenchyma. GTE/ZnO NPs act as partial hepato-protective against MSG.

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The impact of zinc oxide nanoparticles on nitrification and the bacterial community in activated sludge in an SBR

RSC Advances ◽

10.1039/c5ra07106b ◽

2015 ◽

Vol 5 (82) ◽

pp. 67335-67342 ◽

Cited By ~ 19

Author(s):

S. T. Wang ◽

S. P. Li ◽

W. Q. Wang ◽

H. You

Keyword(s):

Zinc Oxide ◽

Bacterial Community ◽

Activated Sludge ◽

Zinc Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Zno Nps ◽

The Impact

20 and 50 mg L−1 of ZnO NPs affect transformation of NH4+–N to NO2−–N and NO2−–N to NO3−–N.

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Long-Term Impact of Zinc Oxide Nanoparticles on Differentiation and Cytokine Secretion of Human Adipose-Derived Stromal Cells

Materials ◽

10.3390/ma12111823 ◽

2019 ◽

Vol 12 (11) ◽

pp. 1823 ◽

Cited By ~ 2

Author(s):

Katrin Radeloff ◽

Andreas Radeloff ◽

Mario Ramos Tirado ◽

Agmal Scherzad ◽

Rudolf Hagen ◽

...

Keyword(s):

Gene Expression ◽

Zinc Oxide ◽

Stromal Cells ◽

Zinc Oxide Nanoparticles ◽

Caspase 3 ◽

Cytokine Secretion ◽

Oxide Nanoparticles ◽

Zno Nps ◽

The Impact

Zinc oxide nanoparticles (ZnO-NPs) are widely utilized, for example in manufacturing paints and in the cosmetic industry. In addition, there is raising interest in the application of NPs in stem cell research. However, cytotoxic, genotoxic and pro-inflammatory effects were shown for NPs. The aim of this study was to evaluate the impact of ZnO-NPs on cytokine secretion and differentiation properties of human adipose tissue-derived stromal cells (ASCs). Human ASCs were exposed to the subtoxic concentration of 0.2 µg/mL ZnO-NPs for 24 h. After four weeks of cultivation, adipogenic and osteogenic differentiation procedures were performed. The multi-differentiation potential was confirmed histologically and using polymerase chain reaction (PCR). In addition, the gene expression of IL-6, IL-8, vascular endothelial growth factor (VEGF) and caspase 3 was analyzed. Over the course of four weeks after ZnO-NPs exposure, no significant differences were detected in the gene expression of IL-6, IL-8, VEGF and caspase 3 compared to non-exposed cells. The differentiation was also not affected by the ZnO-NPs. These findings underline the fact, that functionality of ASCs is likely to be unaffected by ZnO-NPs, despite a long-term disposition of NPs in the cells, supposing that the starting concentration was safely in the non-toxic range. This might provide important information for single-use nanomedical applications of ZnO-NPs.

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Role of Autophagy in Zinc Oxide Nanoparticles-Induced Apoptosis of Mouse LEYDIG Cells

International Journal of Molecular Sciences ◽

10.3390/ijms20164042 ◽

2019 ◽

Vol 20 (16) ◽

pp. 4042 ◽

Cited By ~ 8

Author(s):

Jingcao Shen ◽

Dan Yang ◽

Xingfan Zhou ◽

Yuqian Wang ◽

Shichuan Tang ◽

...

Keyword(s):

Oxidative Stress ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Leydig Cells ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Sperm Density ◽

Induced Apoptosis

Zinc oxide nanoparticles (ZnO NPs) have shown adverse health impact on the human male reproductive system, with evidence of inducing apoptosis. However, whether or not ZnO NPs could promote autophagy, and the possible role of autophagy in the progress of apoptosis, remain unclear. In the current study, in vitro and in vivo toxicological responses of ZnO NPs were explored by using a mouse model and mouse Leydig cell line. It was found that intragastrical exposure of ZnO NPs to mice for 28 days at the concentrations of 100, 200, and 400 mg/kg/day disrupted the seminiferous epithelium of the testis and decreased the sperm density in the epididymis. Furthermore, serum testosterone levels were markedly reduced. The induction of apoptosis and autophagy in the testis tissues was disclosed by up-regulating the protein levels of cleaved Caspase-8, cleaved Caspase-3, Bax, LC3-II, Atg 5, and Beclin 1, accompanied by down-regulation of Bcl 2. In vitro tests showed that ZnO NPs could induce apoptosis and autophagy with the generation of oxidative stress. Specific inhibition of autophagy pathway significantly decreased the cell viability and up-regulated the apoptosis level in mouse Leydig TM3 cells. In summary, ZnO NPs can induce apoptosis and autophagy via oxidative stress, and autophagy might play a protective role in ZnO NPs-induced apoptosis of mouse Leydig cells.

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Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis

Biomolecules ◽

10.3390/biom10060918 ◽

2020 ◽

Vol 10 (6) ◽

pp. 918 ◽

Cited By ~ 1

Author(s):

Afifa Zaeem ◽

Samantha Drouet ◽

Sumaira Anjum ◽

Razia Khurshid ◽

Muhammad Younas ◽

...

Keyword(s):

Oxidative Stress ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

In Vitro Cultures ◽

Total Phenolic ◽

Oxide Nanoparticles ◽

Stem Explants ◽

Enhanced Production ◽

The Impact

Linum usitatissimum biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem-derived callus of L. usitatissimum. Seedlings of L. usitatissimum grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol-β-coniferyl alcohol ether glucoside in the plantlets grown on the 500 mg/L ZnONPs. On the other hand, the stem explants were cultured on MS media comprising 1-naphthaleneacetic acid (1 mg/L) and ZnONPs (1–50 mg/L). The highest antioxidant and other activities with an enhanced rooting effect were noted in 25 mg/L ZnONP-treated callus. Similarly, the maximum metabolites were also accumulated in 25 mg/L ZnONP-treated callus. In both systems, the dose-dependent production of reactive oxygen species (ROS) was recorded, resulting in oxidative damage with a more pronounced toxic effect on in vitro cultures. Altogether, the results from this study constitute a first comprehensive view of the impact of ZnONPs on the oxidative stress and antioxidant responses in seedlings vs. in vitro cultures.

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Beneficial Effects of Zinc Oxide Nanoparticles on Plant Regeneration of Vetiver Grass (Vetiveria zizanioides L. Nash)

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.866.25 ◽

2017 ◽

Vol 866 ◽

pp. 25-28

Author(s):

Chonnikarn Khunchuay ◽

Kanokporn Sompornpailin

Keyword(s):

Zinc Oxide ◽

Plant Regeneration ◽

Zinc Oxide Nanoparticles ◽

Shoot Length ◽

Optimal Concentration ◽

Oxide Nanoparticles ◽

Vetiver Grass ◽

Vetiveria Zizanioides ◽

Zno Nps ◽

Positive Effects

Zinc is an essential micronutrient element for plant. Nowadays, Zinc oxide nanoparticles (ZnO NPs) are widely used and have been shown adverse effects to plant physiology. The optimal concentration of ZnO NPs added in plant regeneration medium (PRM) is studied in this experiment. Six-weeks old calli induced from the aseptic axillary buds of vetiver grass were cultured in PRM with various concentrations of ZnO NPs (5, 10, 20, 40 mgL-1). PRM containing 5, 10 mgL-1 ZnO NPs showed the highest percentage of plant regeneration frequency (95%) but did not have a statistic difference with other treatments. However, the averages of shoot length regenerated under both concentrations were 1.32-1.33 cm. These averages of shoot length had statistically different from averages of shoot length of the others. Moreover, calli regenerated under PRM containing 5 mgL-1 ZnO NPs condition showed the highest average number of shoots per callus (9.75 shoots) with a statistic difference. ZnO NPs at 20-40 mgL-1 in the PRM did not enhance the percentage of plant regeneration, but retarded calli development and shoot elongation. The optimal concentration of ZnO NPs in PRM has positive effects on regeneration and development of vetiver grass under tissue culture condition.

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