scholarly journals How well do genetic markers inform about responses to intraspecific admixture? A comparative analysis of microsatellites and RADseq

2021 ◽  
Author(s):  
Yeşerin Yıldırım ◽  
Anders Forsman ◽  
Johanna Sunde
Keyword(s):  
Genetic Similarity ◽  
Biological Diversity ◽  
Hatching Success ◽  
Genetic Admixture ◽  
Published Data ◽  
Negative Consequences ◽  
Offspring Performance ◽  
Genome Wide ◽  
Neutral Marker ◽  
And Performance

Abstract Background: Fitness consequences of intraspecific genetic admixture can vary from positive to negative depending on the genetic composition of the populations and environmental conditions. Because admixture has potential to influence the success of management and conservation efforts, genetic similarity has been suggested to be used as a proxy to predict the outcome. Studies utilizing microsatellites (a neutral marker) to investigate associations between genetic distance and admixture effects show conflicting results. Marker types that yield information on genome-wide and/or adaptive variation might be more useful for predicting responses to inter-population hybridization. In this study we utilized published data for three populations of pike (Esox lucius) to investigate associations between offspring performance (hatching success) and parental genetic similarity in experimentally purebred and admixed families, based on neutral (microsatellites), genome-wide neutral (RADseq SNPs), and adaptive (SNPs under selection) markers.Results: Estimated similarity varied among the markers, likely reflecting differences in their inherent properties, but was consistently higher in purebred than admixed families. A significant interaction between marker type and admixture treatment reflected that neutral SNPs yielded higher estimates than adaptive SNPs for admixed families whereas no difference was found for purebred families, which indicates that neutral similarity was not reflective of adaptive similarity. When all samples were pooled, no association between similarity and performance was found for any marker. For microsatellites, similarity was positively correlated with hatching success in purebred families, whereas no association was found in admixed families; however, the direction of the effect differed between the population combinations. Conclusions: The results strengthen the notion that, as of today, there is no proxy that can reliably predicted the outcome of admixture. This emphasizes the need of further studies to advance knowledge that can shed light on how to safeguard against negative consequences of admixture, and thereby inform management and promote conservation of biological diversity.

scholarly journals How well do genetic markers inform about responses to intraspecific admixture? A comparative analysis of microsatellites and RADseq

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yeşerin Yıldırım ◽  
Anders Forsman ◽  
Johanna Sunde
Keyword(s):  
Genetic Similarity ◽  
Biological Diversity ◽  
Hatching Success ◽  
Genetic Admixture ◽  
Published Data ◽  
Negative Consequences ◽  
Offspring Performance ◽  
Genome Wide ◽  
Neutral Marker ◽  
And Performance

Abstract Background Fitness consequences of intraspecific genetic admixture can vary from positive to negative depending on the genetic composition of the populations and environmental conditions. Because admixture has potential to influence the success of management and conservation efforts, genetic similarity has been suggested to be used as a proxy to predict the outcome. Studies utilizing microsatellites (a neutral marker) to investigate associations between genetic distance and admixture effects show conflicting results. Marker types that yield information on genome-wide and/or adaptive variation might be more useful for predicting responses to inter-population hybridization. In this study we utilized published data for three populations of pike (Esox lucius) to investigate associations between offspring performance (hatching success) and parental genetic similarity in experimentally purebred and admixed families, based on neutral (microsatellites), genome-wide neutral (RADseq SNPs), and adaptive (SNPs under selection) markers. Results Estimated similarity varied among the markers, likely reflecting differences in their inherent properties, but was consistently higher in purebred than admixed families. A significant interaction between marker type and admixture treatment reflected that neutral SNPs yielded higher estimates than adaptive SNPs for admixed families whereas no difference was found for purebred families, which indicates that neutral similarity was not reflective of adaptive similarity. When all samples were pooled, no association between similarity and performance was found for any marker. For microsatellites, similarity was positively correlated with hatching success in purebred families, whereas no association was found in admixed families; however, the direction of the effect differed between the population combinations. Conclusions The results strengthen the notion that, as of today, there is no proxy that can reliably predicted the outcome of admixture. This emphasizes the need of further studies to advance knowledge that can shed light on how to safeguard against negative consequences of admixture, and thereby inform management and promote conservation of biological diversity.

Effects of water deficiency on preference and performance of an insect herbivore Ostrinia furnacalis

2021 ◽  
pp. 1-10
Author(s):  
M.Y. Duan ◽  
H. Zhu ◽  
H. Wang ◽  
S.Y. Guo ◽  
H. Li ◽  
...  
Keyword(s):  
Driving Forces ◽  
Oviposition Preference ◽  
Precipitation Pattern ◽  
Ostrinia Furnacalis ◽  
Oviposition Choice ◽  
Offspring Performance ◽  
Corn Borer ◽  
Limited Mobility ◽  
Offspring Fitness ◽  
And Performance

Abstract With further climate change still expected, it is predicted to increase the frequency with plants will be water stressed, which subsequently influences phytophagous insects, particularly Lepidoptera with limited mobility of larvae. Previous studies have indicated that oviposition preference and offspring performance of Lepidoptera insects are sensitive to drought separately. However, the integration of their two properties is not always seen. Here, we evaluated changes in oviposition selection and offspring fitness of a Lepidoptera insect under three water-stressed treatments using a model agroecosystem consisting of maize Zea mays, and Asian corn borer Ostrinia furnacalis. Results found that female O. furnacalis preferred to laying their eggs on well-watered maize, and then their offspring tended to survive better, attained bigger larvae mass, and developed more pupae and adults on the preferred maize. Oviposition selection of O. furnacalis positively correlated with height and leaf traits of maize, and offspring fitness positively related with water content and phytochemical traits of hosts. Overall, these results suggest that oviposition choice performed by O. furnacalis reflects the maximization of offspring fitness, supporting preference–performance hypothesis. This finding further highlights that the importance of simultaneous evaluation of performance and performance for water driving forces should be involved, in order to accurately predict population size of O. furnacalis under altered precipitation pattern.

scholarly journals Extended Evaluation of Viral Diversity in Lake Baikal through Metagenomics

2021 ◽  
Vol 9 (4) ◽  
pp. 760
Author(s):  
Tatyana V. Butina ◽  
Yurij S. Bukin ◽  
Ivan S. Petrushin ◽  
Alexey E. Tupikin ◽  
Marsel R. Kabilov ◽  
...  
Keyword(s):  
Lake Baikal ◽  
Biological Diversity ◽  
Anthropogenic Impacts ◽  
Lake Ecosystem ◽  
Published Data ◽  
Full Diversity ◽  
True Diversity ◽  
Viral Communities ◽  
Diversity Studies ◽  
Insight Into

Lake Baikal is a unique oligotrophic freshwater lake with unusually cold conditions and amazing biological diversity. Studies of the lake’s viral communities have begun recently, and their full diversity is not elucidated yet. Here, we performed DNA viral metagenomic analysis on integral samples from four different deep-water and shallow stations of the southern and central basins of the lake. There was a strict distinction of viral communities in areas with different environmental conditions. Comparative analysis with other freshwater lakes revealed the highest similarity of Baikal viromes with those of the Asian lakes Soyang and Biwa. Analysis of new data, together with previously published data allowed us to get a deeper insight into the diversity and functional potential of Baikal viruses; however, the true diversity of Baikal viruses in the lake ecosystem remains still unknown. The new metaviromic data will be useful for future studies of viral composition, distribution, and the dynamics associated with global climatic and anthropogenic impacts on this ecosystem.

Surgical Drilling: Design and Performance of an Improved Drill

1982 ◽  
Vol 104 (3) ◽  
pp. 245-252 ◽  
Author(s):  
S. Saha ◽  
S. Pal ◽  
J. A. Albright
Keyword(s):  
Compact Bone ◽  
Helix Angle ◽  
Rake Angle ◽  
Peak Temperature ◽  
Published Data ◽  
Drill Bit ◽  
Effective Removal ◽  
Split Point ◽  
And Performance ◽  
Twist Drills

The majority of twist drills used in orthopaedics are very similar to chisel pointed metal drilling bits. Modifications usually observed are reduction of the point angle to 90 deg and sometimes grinding of the entire cutting lip at 0 deg rake angle, which appeared to have been made arbitrarily without any advantage. We have attempted to design a surgical drill bit with the objective of minimization of the drilling thrust and temperature and effective removal of bone chips. Our results showed that the presence of the chisel edge was mainly responsible for increasing the thrust force and the temperature developed. The effects of a constant feed rate and thrust on the peak temperature were also examined. The combined effect of the helix and the point angles on the rake angle which in turn determines the cutting efficiency was analyzed for various types of surgical bits. Based on our results and previously published data from the literature an optimized drill bit was designed with a split point, a point angle of 118 deg, a parabolic flute, and a helix angle of 36 deg and its performance was compared with other existing surgical drill bits. For drilling in compact bone, the new design decreased the thrust load by 45 percent and the peak temperature rise by 41 percent. Simlar improvements were also recorded for drilling bone cement. The time of drilling a bone cortex was also significantly reduced and “walking” on the curved bone surface was eliminated and dimensional tolerance on hole sizes was improved. The new design is likely to reduce the time of surgery and also minimize the tissue damage.

scholarly journals Genome-wide association for heifer reproduction and calf performance traits in beef cattle

Genome ◽  
2015 ◽  
Vol 58 (12) ◽  
pp. 549-557 ◽  
Author(s):  
Everestus C. Akanno ◽  
Graham Plastow ◽  
Carolyn Fitzsimmons ◽  
Stephen P. Miller ◽  
Vern Baron ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Genome Wide Association Study ◽  
Average Daily Gain ◽  
Snp Markers ◽  
Genome Wide Association ◽  
Research Centre ◽  
Genome Wide ◽  
A Genome ◽  
Starting Point ◽  
And Performance

The aim of this study was to identify SNP markers that associate with variation in beef heifer reproduction and performance of their calves. A genome-wide association study was performed by means of the generalized quasi-likelihood score (GQLS) method using heifer genotypes from the BovineSNP50 BeadChip and estimated breeding values for pre-breeding body weight (PBW), pregnancy rate (PR), calving difficulty (CD), age at first calving (AFC), calf birth weight (BWT), calf weaning weight (WWT), and calf pre-weaning average daily gain (ADG). Data consisted of 785 replacement heifers from three Canadian research herds, namely Brandon Research Centre, Brandon, Manitoba, University of Alberta Roy Berg Kinsella Ranch, Kinsella, Alberta, and Lacombe Research Centre, Lacombe, Alberta. After applying a false discovery rate correction at a 5% significance level, a total of 4, 3, 3, 9, 6, 2, and 1 SNPs were significantly associated with PBW, PR, CD, AFC, BWT, WWT, and ADG, respectively. These SNPs were located on chromosomes 1, 5–7, 9, 13–16, 19–21, 24, 25, and 27–29. Chromosomes 1, 5, and 24 had SNPs with pleiotropic effects. New significant SNPs that impact functional traits were detected, many of which have not been previously reported. The results of this study support quantitative genetic studies related to the inheritance of these traits, and provides new knowledge regarding beef cattle quantitative trait loci effects. The identification of these SNPs provides a starting point to identify genes affecting heifer reproduction traits and performance of their calves (BWT, WWT, and ADG). They also contribute to a better understanding of the biology underlying these traits and will be potentially useful in marker- and genome-assisted selection and management.

Comment on Cameron and Cameron (2002): “Children of Homosexual Parents Report Childhood Difficulties”

2002 ◽  
Vol 91 (1) ◽  
pp. 331-332
Author(s):  
Lowell L. Brubaker
Keyword(s):  
Home Environment ◽  
Psychological Trauma ◽  
Parent Report ◽  
Published Data ◽  
Negative Consequences ◽  
Control Groups ◽  
Childhood Difficulties

Cameron and Cameron's reanalysis of published data in 2002 indicates children being raised in a home environment with at least one homosexual parent report some negative consequences. However, a closer look at the information presented suggests (especially in the absence of control groups) that the negative consequences documented do not constitute major psychological trauma. Rather, they are more in the nature of the teasing and bullying that plagues any child who comes from a home that may be atypical in any fashion.

scholarly journals Toward a dynamic model of Gelotophobia: Social support, workplace bullying and stress are connected with diverging trajectories of life and job satisfaction among Gelotophobes

2020 ◽  
Author(s):  
Willibald Ruch ◽  
Alexander G. Stahlmann
Keyword(s):  
Social Support ◽  
Job Satisfaction ◽  
Panel Data ◽  
Work Stress ◽  
Perceived Stress ◽  
Workplace Bullying ◽  
Well Being ◽  
Negative Consequences ◽  
Dynamic Framework ◽  
And Performance

Abstract Recent theoretical advances have grounded gelotophobia (Greek: gelos = laughter, phobos = fear) in a dynamic framework of causes, moderating factors, and consequences of the fear of being laughed at. This understanding corresponds to that of vulnerability and translates gelotophobia into a distinguishable pattern of lacking resources (i.e., misinterpretation of joy and laughter) that can result in negative consequences (e.g., reduced well-being and performance) if individuals have no access to further resources (e.g., social support) or are exposed to severe stressors (e.g., workplace bullying). Based on the panel data provided by the Swiss National Centre of Competence in Research LIVES (N = 2469 across six measurement intervals), this study takes the first step toward empirically testing this model’s assumptions: First, we computed exemplary zero-order correlations and showed that gelotophobia was negatively connected with social support (resource) and life and job satisfaction (consequences) and positively connected with perceived stress, work stress, and workplace bullying (stressors). Second, we used longitudinal cluster analyses (KmL; k-means-longitudinal) and showed that the panel data can be clustered into three stable patterns of life and job satisfaction and that gelotophobia is primarily related to the two clusters marked by lower levels of satisfaction. Third, we computed partial correlations and showed that social support, perceived stress, and work stress (but not workplace bullying) can weaken or completely resolve gelotophobia’s relationships with such diverging trajectories of life and job satisfaction. We concluded that seeing gelotophobia through the lens of vulnerability is useful and that such research warrants further attention using more dedicated, theoretically grounded projects.

scholarly journals Performance Prediction Equation for 2000 m Youth Indoor Rowing Using a 100 m Maximal Test

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1082
Author(s):  
Luiz Felipe da Silva ◽  
Paulo Francisco de Almeida-Neto ◽  
Dihogo Gama de Matos ◽  
Steven E. Riechman ◽  
Victor de Queiros ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Reliability Index ◽  
High Reliability ◽  
Low Cost ◽  
Time Trial ◽  
Prediction Equation ◽  
Daily Routine ◽  
Negative Consequences ◽  
Maximum Effort ◽  
And Performance

Background: The exhaustive series of tests undergone by young athletes of Olympic rowing prior to important competitions imply loads of physical stress that can ultimately impact on mood and motivation, with negative consequences for their training and performance. Thus, it is necessary to develop a tool that uses only the performance of short distances but is highly predictive, offering a time expectancy with high reliability. Such a test must use variables that are easy to collect with high practical applicability in the daily routine of coaches. Objective: The objective of the present study was to develop a mathematical model capable of predicting 2000 m rowing performance from a maximum effort 100 m indoor rowing ergometer (IRE) test in young rowers. Methods: The sample consisted of 12 male rowing athletes in the junior category (15.9 ± 1.0 years). A 100 m time trial was performed on the IRE, followed by a 2000 m time trial 24-h later. Results: The 2000 m mathematical model to predict performance in minutes based on the maximum 100 m test demonstrated a high correlation (r = 0.734; p = 0.006), strong reliability index (ICC: 0.978; IC95%: [0.960; 0.980]; p = 0.001) and was within usable agreement limits (Bland -Altman Agreement: −0.60 to 0.60; 95% CI [−0.65; 0.67]). Conclusion: The mathematical model developed to predict 2000 m performance is effective and has a statistically significant reliability index while being easy to implement with low cost.

scholarly journals Using Communication Boundaries to Minimize Athlete Social Media Distractions During Events

2021 ◽  
Vol 25 (6) ◽  
pp. 683-704
Author(s):  
Michelle Hayes ◽  
Kevin Filo ◽  
Caroline Riot ◽  
Andrea N. Geurin
Keyword(s):  
Social Media ◽  
Best Practice ◽  
Media Use ◽  
Negative Consequences ◽  
Social Media Use ◽  
Communication Privacy Management ◽  
Performance Criticism ◽  
And Performance ◽  
Use Of Social Media ◽  
Sport Events

Sport organizations regulate athletes' use of social media for many reasons including the protection of the organization's reputation. Several strategies have been introduced to minimize issues related to the negative consequences athlete social media use may present, yet whether these strategies also work to address social media distractions experienced by athletes during major sport events is not well known. Utilizing communication privacy management (CPM) theory, the purpose of the current research was to examine the aspects of social media that sport administrators perceive to be distracting to athletes and what support and management mechanisms are utilized to address such concerns during major sport events. Semistructured interviews ( N = 7) with Australian national sport organization (NSO) administrators were conducted. Sport administrators reported several aspects of social media that are perceived to distract athletes including personal and performance criticism and a fixation with social media profiles. Social media could also be used to manage athlete temperament. As a result, organizations highlighted both proactive and reactive communication boundaries and mechanisms that could be used to address concerns including content restrictions, best practice case studies, engaging in conversations, and monitoring. Opportunities for sport practitioners are described including conducting consultation sessions with athletes to better understand their needs regarding their social media use.

scholarly journals Central clock components modulate plant shade avoidance by directly repressing transcriptional activation activity of PIF proteins

2020 ◽  
Vol 117 (6) ◽  
pp. 3261-3269 ◽  
Author(s):  
Yu Zhang ◽  
Anne Pfeiffer ◽  
James M. Tepperman ◽  
Jutta Dalton-Roesler ◽  
Pablo Leivar ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Target Genes ◽  
Direct Action ◽  
Published Data ◽  
Shade Avoidance ◽  
Response Regulators ◽  
Genome Wide ◽  
Promoter Occupancy ◽  
Factor Abundance ◽  
Direct Target

Light-environment signals, sensed by plant phytochrome photoreceptors, are transduced to target genes through direct regulation of PHYTOCHROME-INTERACTING FACTOR (PIF) transcription factor abundance and activity. Previous genome-wide DNA-binding and expression analysis has identified a set of genes that are direct targets of PIF transcriptional regulation. However, quantitative analysis of promoter occupancy versus expression level has suggested that unknown “trans factors” modulate the intrinsic transcriptional activation activity of DNA-bound PIF proteins. Here, using computational analysis of published data, we have identified PSEUDO-RESPONSE REGULATORS (PRR5 and PRR7) as displaying a high frequency of colocalization with the PIF proteins at their binding sites in the promoters of PIF Direct Target Genes (DTGs). We show that the PRRs function to suppress PIF-stimulated growth in the light and vegetative shade and that they repress the rapid PIF-induced expression of PIF-DTGs triggered by exposure to shade. The repressive action of the PRRs on both growth and DTG expression requires the PIFs, indicating direct action on PIF activity, rather than a parallel antagonistic pathway. Protein interaction assays indicate that the PRRs exert their repressive activity by binding directly to the PIF proteins in the nucleus. These findings support the conclusion that the PRRs function as direct outputs from the core circadian oscillator to regulate the expression of PIF-DTGs through modulation of PIF transcriptional activation activity, thus expanding the roles of the multifunctional PIF-signaling hub.

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