scholarly journals Accumulation, Translocation, and Toxicity of Arsenic in Barley Grown in Contaminated Soil

Author(s):  
Marina Burachevskaya ◽  
Tatiana Minkina ◽  
Alexey Fedorenko ◽  
Grigorii Fedorenko ◽  
Natalia Chernikova ◽  
...  

Abstract Aims Arsenic is a nonessential element for plants, however, high levels of As can inhibit plant growth. Toxicity of As is largely influenced by its speciation in soil. The objectives of the present study were to determine fractional composition of As in soil, its accumulation in plants, and toxic effects on the morphological, anatomical, and ultrastructural levels. Methods In a model experiment, barley (Hordeum sativum) was planted in Haplic Chernozem spiked with three different concentrations of As (20, 50 and 100 mg/kg). The fraction composition of As in the experimental soil was analysed using a method of sequential fractionation. The effect of As on plants was analysed microscopically at tissue, cellular, and intracellular levels.Results Analysis of the fraction composition of As revealed a higher amount of mobile forms of As that contaminated the generative organs of plants. Oxides of Fe, Al, and Mn became the main soil components to retain As when contamination of As increased. Arsenic toxicity inhibited plant growth by affecting morphological parameters (shape, size, and colour). It was shown impairment in the root cells and a reduction in the size of the chlorophyllic parenchyma in the leaves. Ultrastructural analysis found changes in the main cellular organelles (chloroplasts, mitochondria, and peroxisomes).Conclusions The bioconcentration factor (BCF), bioaccumulation factor (BF-soluble), and translocation factor (TF) allowed evaluation of plant protection mechanisms and determination of hazardous concentrations of As in soil. Despite high buffering capacity of soil, high As concentration affected morphological and ultrastructural parameters of the H. sativum.

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 912
Author(s):  
Shuming Liu ◽  
Hongmei Liu ◽  
Rui Chen ◽  
Yong Ma ◽  
Bo Yang ◽  
...  

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.


2013 ◽  
Vol 726-731 ◽  
pp. 1755-1759
Author(s):  
Jun Liu ◽  
Jin Xia Nie

In order to study the soil amend agent how to suppress the function of the vegetables absorb Pb, the Pb was added in the experimental soil in pakchoi pot, The lime and peanut dry cake were added to the experimental soil as modifier, and the varying concentrate of modifiers, lead effect on pakchoi biomass and lead accumulate were discussed in this paper. Through the determination of Pb content of aboveground and underground part of pakchoi by ICP, the lime and peanut dry cake can resist lead absorption were proved, and the lime achieved better inhibitory effect of the two. By analyzed the BCF of two parts of pakchoi, two types of modifiers had proved can reduce the pakchoi BCF of Pb in the soil. The lime was more efficient than the peanut dry cake on reducing BCF of Pb.


2021 ◽  
Vol 66 (3) ◽  
Author(s):  
Dipriya R. Lyngkhoi

The present study was undertaken to estimate the costs and returns structure of maize cultivation and identifying the prominent production constraints in West Khasi Hills district of Meghalaya. A sample of 60 farmers was randomly drawn from six villages of Mawthadraishan and Nongstoin block of the selected district. The costs and returns per hectare were calculated on the basis of cost concepts and Garett ranking method was used for employed for determination of constraints in maize production. The overall cost of cultivation was found to be ` 37185.22 per ha and the major cost components were manures (48.25%) and human labour (34.73%). The overall net return was evaluated at ` 19038.20 with small, medium and large farmers having similar returns with the exception of marginal farmers gaining a net return of only ` 13889.83 which was 27.04 per cent lower than the average return among the sample farmers. It may be attributed to their heavy dependence on labour and lack of investment on irrigation, plant protection and better-quality seeds. The realised average yield was found to be 23.65 q/ha which was abysmally low compared to other maize producing states of India. A positive trend between the return over cost ratio and the operational holding was observed with an average of 1.51. The prominent constraints as perceived by the farmers were unfavorable weather conditions, the incidence of pests and diseases and costly fertilizers and manures with the Garrett’s score of 64.70, 62.75 and 54.40 respectively


2018 ◽  
Vol 1 (2) ◽  
pp. 42-52
Author(s):  
Alina Stingu ◽  
Corneliu Tanase ◽  
Valentin I. Popa

Abstract The aim of this study is to evaluate the possibility of using hemp shives as natural amendments in a phytoremediation sequence. Thus, plant growth tests were conducted for rapeseed (Brassica napus) and flax (Linum usitatissimum) in vegetation pots with sandy soil. These were seeded in a cadmium artificial contaminated environment with or without hemp shives. The efficiency of hemp shives as natural amendments was evaluated after 40 days of initiating experimental model, by evaluation of plant growth and development of plants through biometric and gravimetric measurements. Also, the concentration of assimilatory pigments was determined. In order to establish bioaccumulation capacity, degree of recovery and translocation factor, it was determined the concentration of cadmium metal ions absorbed in various parts of rapeseed and flax plants. It has been found that the development of the root system is more pronounced in the presence of hemp shives both in contamination with cadmium ions and in the absence. By determining cadmium concentrations accumulate in various parts of the plant have been shown that supplementing growth media with hemp shives, improves the bioaccumulation process of cadmium ions. According to the results, the hemp shives can be used as amendments in phytoremediation process, enhancing bioaccumulation process.


2021 ◽  
Vol 46 (3) ◽  
pp. 40-48
Author(s):  
Magó László ◽  
Kosta Gligorević ◽  
Milan Dražić ◽  
Mićo Oljača

ISO organization (https://www.iso.org) in early 90s defined an industry standard for the communication protocol among electronic devices of different manufacturers of agricultural machines. After that, all of the market actors recognised that this technology would be very important for agricultural electronics. The appearance of ISOBUS products in the market was in the mid-2000s. ISOBUS description could be found in ISO-11783 (https://www.iso.org). Through the standards and the related technical background, the production processes and the operations could be followed and monitored by the extensive Data Management. Farmers' and users legitimate needs and developing goal is to elaborate a decision support systems that follow-up the utilisation of the machines and ensure the quality of operations. For this purpose, it is essential to determine which technical, economical, technological parameters detection, measurement, transmission, processing, and evaluation becomes necessary. In our work, we reviewed which mechanical characteristic, settings are monitored within the ISOBUS system by the major machine manufacturers. We developed the system of parameters and derived features that provide effective farm-, and land-management in case of attached equipment for spreading of input materials, plant protection and tillage implements.


PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10053
Author(s):  
Fernando Carlos Gómez-Merino ◽  
Libia Iris Trejo-Téllez ◽  
Atonaltzin García-Jiménez ◽  
Hugo Fernando Escobar-Sepúlveda ◽  
Sara Monzerrat Ramírez-Olvera

Background Silicon (Si) is categorized as a quasi-essential element for plants thanks to the benefits on growth, development and metabolism in a hormetic manner. Si uptake is cooperatively mediated by Lsi1 and Lsi2. Nevertheless, Lsi channels have not yet been identified and characterized in pepper (Capsicum annuum), while genes involved in major physiological processes in pepper are Si-regulated. Furthermore, Si and phytohormones may act together in regulating plant growth, metabolism and tolerance against stress. Our aim was to identify potential synergies between Si and phytohormones stimulating growth and metabolism in pepper, based on in silico data. Methods We established a hydroponic system to test the effect of Si (0, 60, 125 and 250 mg L−1 Si) on the concentrations of this element in different pepper plant tissues. We also performed an in silico analysis of putative Lsi genes from pepper and other species, including tomato (Solanum lycopersicum), potato (Solanum tuberosum) and Arabidopsis thaliana, to look for cis-acting elements responsive to phytohormones in their promoter regions. With the Lsi1 and Lsi2 protein sequences from various plant species, we performed a phylogenetic analysis. Taking into consideration the Lsi genes retrieved from tomato, potato and Arabidopsis, an expression profiling analysis in different plant tissues was carried out. Expression of Si-regulated genes was also analyzed in response to phytohormones and different plant tissues and developmental stages in Arabidopsis. Results Si concentrations in plant tissues exhibited the following gradient: roots > stems > leaves. We were able to identify 16 Lsi1 and three Lsi2 genes in silico in the pepper genome, while putative Lsi homologs were also found in other plant species. They were mainly expressed in root tissues in the genomes analyzed. Both Lsi and Si-regulated genes displayed cis-acting elements responsive to diverse phytohormones. In Arabidopsis, Si-regulated genes were transcriptionally active in most tissues analyzed, though at different expressed levels. From the set of Si-responsive genes, the NOCS2 gene was highly expressed in germinated seeds, whereas RABH1B, and RBCS-1A, were moderately expressed in developed flowers. All genes analyzed showed responsiveness to phytohormones and phytohormone precursors. Conclusion Pepper root cells are capable of absorbing Si, but small amounts of this element are transported to the upper parts of the plant. We could identify putative Si influx (Lsi1) and efflux (Lsi2) channels that potentially participate in the absorption and transport of Si, since they are mainly expressed in roots. Both Lsi and Si-regulated genes exhibit cis-regulatory elements in their promoter regions, which are involved in phytohormone responses, pointing to a potential connection among Si, phytohormones, plant growth, and other vital physiological processes triggered by Si in pepper.


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