Up-regulation of Rb by small activating RNA inhibits cell invasion and migration in gastric cancer cells
Abstract Gastric cancer (GC) is a lethal disease that needs further investigation. Recent studies have reported that small activating RNA (saRNA), involved in a process called RNA activation, plays an important role in the development of cancer. We designed three different saRNAs targeting the retinoblastoma (Rb) gene promoter in GC cell lines. Upregulated Rb expression after transfection of the saRNAs was confirmed by PCR and western blotting and resulted in inhibition of GC cell proliferation, migration, and invasion compared with the control groups. There was no significant difference between the negative control saRNA and mock transfection groups. Overall, Rb is a promising novel prognostic biomarker of GC and, due to its role in metastasis, a novel therapeutic target for the clinical management of invasive and metastatic GC.