scholarly journals Diversity of Endo and Exo-Bacteria Associated With Fungi Isolated From Plant Rhizospheres: A Pilot Study

2021 ◽  
Author(s):  
Simone Lupini ◽  
Hang N. Nguyen ◽  
Demosthenes Morales III ◽  
Geoffrey L. House ◽  
Sachin Paudel ◽  
...  
Keyword(s):  
Scientific Community ◽  
Plant Pathogens ◽  
Fungal Hypha ◽  
Closely Related Species ◽  
Potential Implication ◽  
The Past ◽  
Fungal Microbiome ◽  
Plant Promoters ◽  
Fungal Associations

Abstract Background: Over the past several years, the scientific community has described the diversity of microbial communities in a variety of soils associated with plants, but at present, little is known about the specific diversity of the soil fungal microbiome involving bacteria colonizing the surface of fungi (i.e., exo-bacteria) or existing within fungal hypha (i.e., endobacteria). This study aimed to collect, identify, and characterize several fungi and their associated (endo- and exo-) microbiome obtained from the rhizosphere of six different plants. Microcosm devices called fungal highway columns, containing one of four plant-based media as attractants, were placed in the rhizosphere of six different plants. The isolated fungi and their associated endo- and exo- bacteria were identified by sequencing of the ITS (fungi) or 16S (bacteria) rRNA regions, followed by Scanning Electron Microscope (SEM), and fluorescence in situ hybridization (FISH) imaging.Results: Most of the fungi recovered are known plant pathogens, such as Fusarium, Pleosporales, and Cladosporium together with species associated with the soil, e.g. Kalmusia. The exo-bacteria recovered were previously described as plant promoters, such as Bacillus, Rhizobium, Acinetobacter or Ensifer. The interactions between fungi and exo-bacteria recovered from fungal highway columns were further investigated via confrontation assays. From the reconstruction of the potential co-occurring bacterial-fungal associations in the rhizosphere, we discovered that the most promiscuous exo-bacterium group (associated with diverse fungi) was Bacillus. From the study of the endobacterial community, emerged a core of shared endosymbionts with a potential implication in the nitrogen cycle.Conclusions: The present study demonstrated the importance of selecting and studying cultivable fungi and bacteria from the rhizosphere. Our findings demonstrated that at the rhizosphere level, the range of interactions between fungi and bacteria, both internal and external to the fungal hypha, could vary even among closely related species.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

In situ microprobe quantitation of inorganic particle burden in paraffin sections of lung tissue

1988 ◽  
Vol 46 ◽  
pp. 990-991
Author(s):  
Jerrold L. Abraham
Keyword(s):  
Lung Tissue ◽  
Quantitative Information ◽  
Particulate Material ◽  
Paraffin Sections ◽  
Tissue Samples ◽  
Qualitative And Quantitative ◽  
The Past ◽  
Quantitative Analyses ◽  
Data Result

Inorganic particulate material of diverse types is present in the ambient and occupational environment, and exposure to such materials is a well recognized cause of some lung disease. To investigate the interaction of inhaled inorganic particulates with the lung it is necessary to obtain quantitative information on the particulate burden of lung tissue in a wide variety of situations. The vast majority of diagnostic and experimental tissue samples (biopsies and autopsies) are fixed with formaldehyde solutions, dehydrated with organic solvents and embedded in paraffin wax. Over the past 16 years, I have attempted to obtain maximal analytical use of such tissue with minimal preparative steps. Unique diagnostic and research data result from both qualitative and quantitative analyses of sections. Most of the data has been related to inhaled inorganic particulates in lungs, but the basic methods are applicable to any tissues. The preparations are primarily designed for SEM use, but they are stable for storage and transport to other laboratories and several other instruments (e.g., for SIMS techniques).

An atomic-resolution microscope study of Al contracts on GaAs

1986 ◽  
Vol 44 ◽  
pp. 726-727
Author(s):  
Z. Liliental-Weber ◽  
C. Nelson ◽  
R. Ludeke ◽  
R. Gronsky ◽  
J. Washburn
Keyword(s):  
High Speed ◽  
Schottky Contacts ◽  
Detailed Knowledge ◽  
The Past ◽  
Semiconductor Interfaces ◽  
Deposited Metal ◽  
Microwave Applications ◽  
Free Interfaces ◽  
Potential Use

The properties of metal/semiconductor interfaces have received considerable attention over the past few years, and the Al/GaAs system is of special interest because of its potential use in high-speed logic integrated optics, and microwave applications. For such materials a detailed knowledge of the geometric and electronic structure of the interface is fundamental to an understanding of the electrical properties of the contact. It is well known that the properties of Schottky contacts are established within a few atomic layers of the deposited metal. Therefore surface contamination can play a significant role. A method for fabricating contamination-free interfaces is absolutely necessary for reproducible properties, and molecularbeam epitaxy (MBE) offers such advantages for in-situ metal deposition under UHV conditions

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

scholarly journals Understanding ion-induced assembly of cellulose nanofibrilllar gels through shear-free mixing and in situ scanning-SAXS

2021 ◽  
Author(s):  
Tomas Rosén ◽  
Ruifu Wang ◽  
HongRui He ◽  
Chengbo Zhan ◽  
Shirish Chodankar ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Building Block ◽  
Cellulose Nanofibrils ◽  
Advanced Materials ◽  
The Past

During the past decade, cellulose nanofibrils (CNFs) have shown tremendous potential as a building block to fabricate new advanced materials that are both biocompatible and biodegradable. The excellent mechanical properties...

Calibration Methods to Circumvent Unknown Component Spectra for Quantitative in situ Raman Monitoring of Co-Polymerisation Reaction

2021 ◽  
Author(s):  
Wendy Rusli ◽  
Pavan Kumar Naraharisetti ◽  
Wee Chew
Keyword(s):  
Raman Spectroscopy ◽  
Reaction Monitoring ◽  
The Past ◽  
In Situ Raman ◽  
Calibration Methods ◽  
Unknown Component

The use of Raman spectroscopy for reaction monitoring has been successfully applied over the past few decades. One complication in such usage is the applicability for quantitative reaction studies. This...

The Vyredox and Nitredox Methods of in situ Treatment of Groundwater

1988 ◽  
Vol 20 (3) ◽  
pp. 149-163 ◽  
Author(s):  
Carol Braester ◽  
Rudolf Martinell
Keyword(s):  
Water Treatment ◽  
Surface Water ◽  
Traditional Method ◽  
Treatment Method ◽  
Nitrate Treatment ◽  
The Past ◽  
The World ◽  
Nitrate And Nitrite ◽  
Iron And Manganese

Nearly one fifth of all water used in the world is obtained from groundwater. The protection of water has become a high priority goal. During the last decades pollution of water has become more and more severe. Today groundwater is more and more used in comparison with surface water. Recently we have seen accidents, which can pollute nearly all surface water very quickly. Generally the groundwater is easier to protect, as well as cheaper to purify, and above all it is of better quality than the surface water. During the past two decades, alternatives to the traditional method of treating the water in filters have been developed, that is in situ water treatment i.e. the VYREDOX and NITREDOX methods. The most common problem regarding groundwater is too high content of iron and manganese, which can be reduced with the VYREDOX method. In some areas today there are severe problems with pollution by hydrocarbons and nitrate as well, and with modification of the VYREDOX treatment method it is used for hydrocarbon and nitrate treatment as well. The method to reduce the nitrate and nitrite is known as the NITREDOX method.

scholarly journals Scientific literacy, public engagement and responsibility in science

2021 ◽  
pp. 209660832110096
Author(s):  
Daya Reddy
Keyword(s):  
Public Good ◽  
Genome Editing ◽  
Public Engagement ◽  
Scientific Literacy ◽  
Scientific Community ◽  
Global Public Good ◽  
Public Communication ◽  
Digital Revolution ◽  
The Past ◽  
Responsibility Of Scientists

This work addresses the issue of scientific literacy and its connection to the responsibility of scientists in relation to public engagement. The points of departure are, first, the notion of science as a global public good, and, second, developments in the past few decades driven largely by the digital revolution. The latter lend a particular urgency to initiatives aimed at promoting scientific literacy. Arguments are presented for reassessing approaches to public communication. The particular example of genome editing is provided as a vehicle for highlighting the challenges in engagement involving the scientific community, policymakers and broader society.

Did Ground Cover Change Over Geologic Time?

2000 ◽  
Vol 6 ◽  
pp. 171-182 ◽  
Author(s):  
Ben A. LePage ◽  
Hermann W. Pfefferkorn
Keyword(s):  
Fossil Record ◽  
Ground Cover ◽  
The Other ◽  
Detailed Account ◽  
Geologic Time ◽  
The Past ◽  
Other Hand ◽  
Plant Fossil

When one hears the term “ground cover,” one immediately thinks of “grasses.” This perception is so deep-seated that paleobotanists even have been overheard to proclaim that “there was no ground cover before grasses.” Today grasses are so predominant in many environments that this perception is perpetuated easily. On the other hand, it is difficult to imagine the absence or lack of ground cover prior to the mid-Tertiary. We tested the hypothesis that different forms of ground cover existed in the past against examples from the Recent and the fossil record (Table 1). The Recent data were obtained from a large number of sources including those in the ecological, horticultural, and microbiological literature. Other data were derived from our knowledge of Precambrian life, sedimentology and paleosols, and the plant fossil record, especially in situ floras and fossil “monocultures.” Some of the data are original observations, but many others are from the literature. A detailed account of these results will be presented elsewhere (Pfefferkorn and LePage, in preparation).

scholarly journals First in situ record of decadal glacier mass balance (2003–2014) from the Bhutan Himalaya

2016 ◽  
Vol 57 (71) ◽  
pp. 289-294 ◽  
Author(s):  
Phuntsho Tshering ◽  
Koji Fujita
Keyword(s):  
Mass Balance ◽  
Direct Method ◽  
Eastern Himalaya ◽  
Glacier Mass Balance ◽  
Differential Gps ◽  
Humid Climate ◽  
Equilibrium Line Altitude ◽  
The Past ◽  
Southeastern Tibet

AbstractThis study presents the first decadal mass-balance record of a small debris-free glacier in the Bhutan Himalaya, where few in situ measurements have been reported to date. Since 2003 we have measured the mass balance of Gangju La glacier, which covers an area of 0.3km2 and extends from 4900 to 5200ma.s.l., using both differential GPS surveys (geodetic method) and stake measurements (direct method). The observed mass balance ranged from –1.12 to –2.04mw.e. a–1 between 2003 and 2014. The glacier exhibited much greater mass loss than neighbouring glaciers in the eastern Himalaya and southeastern Tibet, which are expected to be sensitive to climate change due to the monsooninfluenced humid climate. Observed mass-balance profiles suggest that the equilibrium-line altitude has been higher than Gangju La glacier since 2003, implying that the entire glacier has experienced net ablation for at least the past decade.

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