scholarly journals Intracellular reactive oxygen species scavenging activity and lipid peroxidation inhibition by secondary metabolites isolated from the endophytic fungus, Daldinia eschscholtzii

2021 ◽  
Author(s):  
Shazia Anjum Musthafa ◽  
Muhammad Sadiq ◽  
Kesavan Muthu ◽  
Saraniya Sekar ◽  
Dhamodharan Umapathy ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Fungal Endophytes ◽  
Radical Scavenging ◽  
Its2 Sequence ◽  
Pituitary Cells ◽  
Alternative Source ◽  
Medicinal Value ◽  
Anti Inflammatory ◽  
Lipid Peroxidation Inhibition ◽  
First Time

Abstract Fungal endophytes associated with medicinal plants are of research interests, since they provide a viable alternative source for molecules with medicinal value. In this study, we report for the first time two fungal endophytic isolates, AI.EF 001 and AI.EF 002 belonging to the genus Daldinia from the leaves of medicinal plant, Abutilon indicum linn (AI). Both AI.EF were identified as Daldinia eschscholtzii (DE) species by ITS1-5.8-ITS2 sequence analysis and phylogenetic tree reconstruction by Neighbor-joining method. Crude extracts of DE (EFEA), generated by ethylacetate/water fractionation of the fungal methanol extracts when subjected to column chromatography separation yielded 5 compounds. NMR and other spectral data revealed the compounds to be ɑ-napthoflavone, Syringaldehyde, 3,4,5-trimethoxy benzoic acid, 2-Furoic acid and Gossypetin 3′ O glycoside. All of these compounds are being reported for the first time from DE. The isolated compounds showed promising free radical scavenging activities. The compounds also exhibited anti inflammatory property by down regulating intracellular ROS as well as inhibiting LPS induced lipid peroxidation in AtT 20 mouse pituitary cells. Current finding demonstrates endophyte DE as a new source for the flavanoid, Gossypetin-3′-O-glycoside along with other phytoconstituents with strong antioxidant and anti-inflammatory activity.

The antioxidative-activity stability of aloe vera (Aloe vera var. chinensis) instant during storage

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 288-293
Author(s):  
Riyanto ◽  
Ch. Wariyah
Keyword(s):  
Lipid Peroxidation ◽  
Critical Condition ◽  
Antioxidative Activity ◽  
Radical Scavenging ◽  
Aloe Vera ◽  
Oxygen Absorption ◽  
Inlet Temperature ◽  
Outlet Temperature ◽  
Plastic Film ◽  
Lipid Peroxidation Inhibition

Aloe vera contains a phenolic compound that has bioactive activity. Previous research showed that microencapsulation of aloe vera powder with maltodextrin as an encapsulation agent produced instant aloe vera with high antioxidative activity. The problem was the hygroscopic instant caused rapid moisture and oxygen absorption during storage, therefore decreasing the instant aloe vera antioxidative activity periodically. The aim of this research was to evaluate the antioxidative activity stability of instant aloe vera during storage. The processing of instant aloe vera through a reconstituted aloe vera powder with water with a ratio of 1:120 and then added with 2.5% maltodextrin as the encapsulating agent. The solution was then inserted into a spray dryer with an inlet temperature of 130oC, an outlet temperature of 103oC, and the flow rate of the solution is 350.0 mL/h. The resulted instant aloe vera was divided into 15 packs with a weight of 25 g, and each sample was wrapped with polyethylene plastic film with 0.80 mm thickness and then was stored at 25oC with a relative humidity of 75%. The sample was conducted in triplicate. The moisture content, and antioxidative activity that was based on the ability to capture 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical (RSA) and lipid peroxidation inhibition were analyzed every week until the critical condition was achieved at a moisture level of 12%. The research showed that the radical scavenging activity (RSA) and lipid peroxidation inhibition of instant aloe vera before storage were 16.34±1.22% and 39.33±1.68%, respectively, whereas in the critical condition the RSA was 3.63±0.04% and the lipid peroxidation inhibition was 22.31±0.02%. Based on their antioxidative activity, the appropriate storage time of instant aloe vera was about 12 weeks in polyethylene plastic film of 0.08 mm thickness

scholarly journals In vitro inhibitory potential of avocado fruits, Persea americana (Lauraceae) against oxidation, inflammation and key enzymes linked to skin diseases

2021 ◽  
Vol 69 (2) ◽  
Author(s):  
Muhammed M. Hürkul ◽  
Sezen Yilmaz Sarialtin ◽  
Ayşegül Köroğlu ◽  
Tülay Çoban
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Skin Diseases ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Persea Americana ◽  
Methanol Extracts ◽  
Hexane Extracts ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Activities

Avocado (Persea americana Mill.) is a member of Lauraceae with one-seeded berry fruit and cultivated in all tropical, subtropical regions in the world and in the Southern coast region of Turkey. Oxidative damage caused by UV can trigger inflammation, resulting in serious inflammatory skin diseases including eczema, seborrheic dermatitis, hyperpigmentation and ageing. Enzyme inhibitors involved in melanogenesis, such as tyrosinase, have been used recently for hyperpigmentation and skin diseases in cosmetic products. Objective: This study aimed to evaluate the antioxidant, anti-inflammatory, anti-tyrosinase activities and total polyphenolic contents of the different parts of P. americana fruit. Methods: The fruit was divided into exocarp, mesocarp, seed, and then methanol and n-hexane extracts were prepared. DPPH and ABTS free radical scavenging capacities and inhibitory potentials on lipid peroxidation were determined to investigate the antioxidant potentials of the extracts. Anti-inflammatory activities of the extracts were evaluated by measuring the stabilization level of the human red blood cell membrane. The tyrosinase inhibitory activities of the samples were determined using mushroom tyrosinase. Results: In general methanol extracts possessed remarkable higher DPPH free radical scavenging activities than n-hexane extracts. The highest activity was determined in methanol extracts of seed (4.17 ± 0.04 mg/mL) followed by exocarp (5.25 ± 0.05 mg/mL). Overall methanol extracts possessed higher ABTS free radical scavenging activities than n-hexane extracts. The greatest ABTS free radical scavenging activity was obtained in methanol extracts of seed (0.03 ± 0.01 mg/mL). In the anti-lipid peroxidation assay, the greatest activity was noticed in methanol extracts of seed (7.71 ± 0.36 µg/mL) followed by exocarp (12.12 ± 0.34 µg/mL), while all n-hexane extracts were inactive. Overall methanol extracts exhibited higher anti-inflammatory and antioxidant properties than n-hexane extracts. However, the maximum anti-tyrosinase activity was determined in n-hexane extracts of exocarp (0.40 ± 0.01 mg/mL) followed by seed (0.46 ± 0.01 mg/mL). Conclusions: These extracts are promising candidates for use as natural products-based antioxidant and anti-inflammatory properties in inflammation-related disease, and also anti-tyrosinase properties in dermatological applications.

scholarly journals Comparative Studies on Anti-Inflammatory, Antioxidant and Antimutagenic Activities of Crassocephalum crepidioides (Bent) Leaf Cold and Hot Water Extracts

2019 ◽  
pp. 1-12 ◽  
Author(s):  
B. A. Akinpelu ◽  
A. Godwin ◽  
T. Gbadegesin ◽  
N. Ajakaye ◽  
S. E. Omotosho ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Radical Scavenging ◽  
Hot Water ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Phenolic Contents ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Aim: To investigate the anti-inflammatory, anti-oxidant and genotoxicity activities of Crassocephalum crepidioides leaf. Study Design: Comparative investigations of the medicinal value and toxicity profile of cold water (CW) and hot water (HW) extracts of C. crepidioides leaf. Place and Duration of Study: Biochemistry and Molecular Biology Department, Obafemi Awolowo University, Ile-Ife. January 2015-October 2016. Materials and Methods: CW and HW of C. crepidioides were analyzed for anti-inflammatory activity via red blood cell membrane stabilization technique and in vitro methods using DPPH radical scavenging activity, thiobarbituric acid-reactive substances (TBARS), ferric reducing antioxidant power (FRAP) and inhibition of oxidative haemolysis were employed to evaluate the antioxidant property. Allium cepa chromosomal assay was adopted to investigate the genotoxic effect of the extracts. Total flavonoid and phenolic contents of the extracts were estimated spectrophotometrically. Results: Both extracts stabilized stressed red blood cell membranes with maximum percentage stability of 50.97±0.06 and 90.90±0.02 at 0.5 and 2.0 mg/ml for CW and HW extracts respectively. The CW extract elicited no DPPH radical scavenging (IC50 -0.63±0.02 mg/ml) and lipid peroxidation (IC50 -0.32±0.00) activities. HW extract had IC50 of 0.29±0.02 and 0.17±0.00 mg/ml for DPPH and lipid peroxidation. CW and HW extracts exhibited FRAP activity of 1186.96±0.01 and 1015.54±0.01 µmol AAE/g respectively. CW extract displayed a weaker protection (29.01±0.01%) against oxidative haemolysis compared to HW extract (68.70 ± 0.00%). CW extract contained higher phenolic contents (2.16±0.03 µmolGAE/g extract) while the HW extract contained higher flavonoids (0.61±0.05 µmolQE/g extract). CW and HW extracts inhibited A. cepa root growth to 71.40±0.02 and 59.10±0.02% respectively. A. cepa mitotic index was reduced to 8.85±0.01 and 8.67±0.02 for CW and HW extracts as compared with control (26.62%). Conclusion: The study concluded that consumption of C. crepidioides leaf in cooked form has more medicinal values however, both CW and HW extracts are capable of causing cellular damage at high doses.

scholarly journals GC-MS Analysis of the Antioxidant Active Fractions of Micromeria juliana with Anticholinesterase Activity

2009 ◽  
Vol 4 (9) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Mehmet Öztürk ◽  
Ufuk Kolak ◽  
Mehmet Emin Duru ◽  
Mansur Harmandar
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Light Petroleum ◽  
Inhibition Activity ◽  
Total Antioxidant ◽  
Lipid Peroxidation Inhibition ◽  
Β Carotene

The aerial parts of Micromeria juliana (L.) Bentham ex Reichb. were extracted with light petroleum, acetone and methanol, successively. The antioxidant activity of different concentrations of the extracts was evaluated using different antioxidant tests, namely total antioxidant (lipid peroxidation inhibition activity), DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging, ferric reducing power, and metal chelating. Total antioxidant activity was determined using the β-carotene-linoleic acid assay. Unexpectedly, the light petroleum extract exhibited strong lipid peroxidation inhibition activity. The extract was fractionated on a silica gel column and the antioxidant activity of the fractions was determined by the β-carotene-linoleic assay at 25 μg/mL concentration. The fractions that exhibited more than 50% inhibition activity were analysed by GC and GC/MS; thus, the structure of fourteen compounds were elucidated. In addition, acetyl- and butyrylcholinesterase inhibitory activities of the extracts were also determined in vitro. The light petroleum and acetone extracts were found to have mild butyrylcholinesterase inhibitory activity.

scholarly journals The Ethanol Fraction of White Rose Petal Extract Abrogates Excitotoxicity-Induced Neuronal Damage In Vivo and In Vitro through Inhibition of Oxidative Stress and Proinflammation

Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1375 ◽  
Author(s):  
Jung-Min Yon ◽  
Yun-Bae Kim ◽  
Dongsun Park
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Neuronal Damage ◽  
Neuroprotective Effect ◽  
Radical Scavenging ◽  
Cresyl Violet ◽  
Anti Inflammatory ◽  
White Rose ◽  
The Brain

Since oxidative stress and inflammation are involved in seizure-related neurotoxicity, the neuroprotective effect of a white rose (Rosa hybrida) petal extract (WRPE) in mice that are challenged with kainic acid (KA) were examined using behavioral epileptiform seizures as well as biochemical and morphological parameters of oxidative stress and inflammation. WRPE (50–200 mg/kg) was orally administered to male ICR mice for 15 days, and intraperitoneally challenged with KA (30 mg/kg). Seizure activity, lipid peroxidation, inflammatory cytokines, and related enzymes were analyzed in the brain tissue, in addition to the morphological alterations in the hippocampal pyramidal neurons. Separately, antioxidant ingredients in WRPE were analyzed, and antioxidant, anti-inflammatory, and neuroprotective activities of WRPE were investigated in HB1.F3 human neural stem cells (NSCs) to elucidate underlying mechanisms. Total polyphenol and flavonoid contents in WRPE were 303.3 ± 15.3 mg gallic acid equivalent/g extract and 18.5 ± 2.2 mg catechin/g extract, respectively. WRPE exhibited strong radical-scavenging activities and inhibited lipid peroxidation in vitro, and protected glutamate-induced cytotoxicity in NSCs by suppressing inflammatory process. Treatment with WRPE attenuated epileptiform seizure scores to a half level in KA-challenged mice, and decreased hippocampal pyramidal neuronal injury and loss (cresyl violet and DAPI staining) as well as astrocyte activation (GFAP immunostaining). Lipid peroxidation was inhibited, and mRNA expression of antioxidant enzymes (GPx, PHGPx, SOD1, and SOD2) were recovered in the brain tissues. Inflammatory parameters (cytokines and enzymes) including NF-kB, IL-1β, TNF-α, IL-6, HMGB1, TGF-β, iNOS, COX2, and GFAP mRNAs and proteins were also down-regulated by WRPE treatment. Taken together, the results indicate that WRPE could attenuate KA-induced brain injury through antioxidative and anti-inflammatory activities.

Contribution of Phenolics and Flavonoids to Anti-Oxidat Activity and of Ethanol Extract from Eichharnia crassipes

2010 ◽  
Vol 156-157 ◽  
pp. 1372-1377 ◽  
Author(s):  
Cheng Chu Liu ◽  
Guo Ling Zhao ◽  
Ya Ning Li ◽  
Zhuo Ping Ding ◽  
Qi Gen Liu ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Red Tide ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Dpph Radical ◽  
Ethanol Extracts ◽  
Lipid Peroxidation Inhibition ◽  
Dpph Radical Scavenging

The polyphenol and flavonoid contents and antioxidation capabilities, including total reducing power, DPPH radical scavenging capability and lipid peroxidation inhibition, of ethanol extracts of water hyacinth (Eichhomia crassipes) were investigated and compared with those extracted from red tide seaweed (Enteromorpha prolifera) and from the most common seaweed (Porphyra haitanensis). Results showed that E. crassipes contained higher amounts of polyphenols [6.68 mg gallic acid equivalents (GAE)/g] in the leaf than in the stem (4.37 mg GAE/g) or in P. haitanensis (4.27 mg GAE/g). The E. crassipes also contained higher contents of flavonoids [1524 and 453 mg rutin equivalents (RE)/g in leaf and stem, respectively] than did E. prolifera (233 mg RE/g). Accordingly, both the leaf and stem of E. crassipes had relatively higher reducing power [118.35 and 47.21 100μg ascorbic acid equivalents (AscAE)/g, respectively] and DPPH radical scavenging capability (74.6% for leaf and 62.7% for stem) when compared with E. prolifera (reducing power of 16.5 and DPPH radical scavenging capability of 42.96%). Statistical analysis showed that the antioxidation capabilities, especially reducing power, of extracts of the three aquatic plants were positively correlated with their total polyphenol contents, but not with the contents of flavonoids. The correlation coefficient (r) of the content of polyphenols and reducing power of those extracts was r=0.9028, that for DPPH scavenging and lipid peroxidation inhibition was r=0.9311 and 0.9099, respectively. These results indicated that phenolic compounds are the main compounds contributing to the antioxidation activities of seaweed extracts. E. crassipes can be a source for extracting natural antioxidants.

Antioxidant Activity of Hizikia fusiformis on Reactive Oxygen Species Scavenging and Lipid Peroxidation Inhibition

2003 ◽  
Vol 9 (5) ◽  
pp. 339-346 ◽  
Author(s):  
Nalin Siriwardhana ◽  
K.-W. Lee ◽  
Y.-J. Jeon ◽  
S.-H. Kim ◽  
J.-W. Haw
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Hydroxyl Radical ◽  
Radical Scavenging ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Ros Scavenging ◽  
Hizikia Fusiformis ◽  
Lipid Peroxidation Inhibition

Water and organic extracts (diethyl ether, chloroform, ethyl acetate, acetone, ethanol and methanol) obtained from Hizikia fusiformis were screened on reactive oxygen species (ROS) scavenging assays (1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydrogen peroxide and hydroxyl radical) and lipid peroxidation (inhibition of linoleic acid oxidation) inhibitory assays. Water, methanol and ethanol extracts showed significant ROS radical scavenging activities. Water extracts showed high scavenging activities on hydrogen peroxide (around 76%) and DPPH radicals (around 75%) while it presented a moderate scavenging activity on hydroxyl radicals (around 54%). Comparatively higher ROS scavenging activities were recorded in hydroxyl radical and DPPH scavenging assays. DPPH radical scavenging activities were well correlated with the polyphenolic content. ROS scavenging and lipid peroxidation inhibition activities indicated that H. fusiformis might be a valuable natural antioxidative source containing both water and fatsoluble antioxidative components.

Lipophilic and hydrophilic antioxidants, lipid peroxidation inhibition and radical scavenging activity of two Lamiaceae food plants

2010 ◽  
Vol 112 (10) ◽  
pp. 1115-1121 ◽  
Author(s):  
Ângela S. F. Fernandes ◽  
Lillian Barros ◽  
Ana Maria Carvalho ◽  
Isabel C. F. R. Ferreira
Keyword(s):  
Lipid Peroxidation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Food Plants ◽  
Scavenging Activity ◽  
Lipid Peroxidation Inhibition ◽  
Hydrophilic Antioxidants
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