scholarly journals Soluble P-selectin and Correlation with Prothrombin Fragment 1+2 in Myeloid Malignancies in Cipto Mangunkusumo General Hospital

2021 ◽  
Author(s):  
Lugyanti Sukrisman
Keyword(s):  
Myeloid Leukemia ◽  
Complete Blood Count ◽  
Leukocyte Count ◽  
Thrombus Formation ◽  
Cross Sectional ◽  
Prothrombin Fragment ◽  
Antiplatelet Medication ◽  
Increased Risk ◽  
Soluble P ◽  
Risk Of Cancer

Abstract Background Patients with hematological malignancies carry increased risk of cancer-associated thrombosis and adhesion molecule plays an important role in the mechanism of thrombus formation. The study aimed to evaluate the soluble P-selectin and prothrombin fragment 1 + 2 (F1 + 2) and to determine the correlation of sP-selectin with leukocyte count and F1 + 2 levels in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) patients. Methods A cross-sectional study of newly diagnosed AML and CML patients, aged 18 years or older. The exclusion criteria were pregnancy, severe infection, immobilization for at least 3 days or during intensive chemotherapy. Controls were healthy people, without anticoagulant and/or antiplatelet medication. Complete blood count was measured by ABX Micros 60®, and sP-selectin and F1 + 2 levels were determined using Behring ELISA Processor-III® and Behring Enzygnost F1 + 2. Results A total of 55 subjects, consisted of 25 AML and 13 CML patients and 17 controls. Soluble P-selectin was significantly elevated in AML patients compared to CML patients (median 0.14 vs 0.25 ng/mL, p = 0.001). Levels of F1 + 2 in AML (median 519.03 pmol/L) and CML patients (median 370.16 pmol/L) were significantly increased compared to controls (p < 0.001 and p = 0.043). Soluble P-selectin were significantly correlated to leukocyte count (R = 0.437; p = 0.029) and F1 + 2 (R = 0.436; p = 0.029) in AML, but not in CML patients. Conclusions AML and CML patients had increased expression of sP-selectin and coagulation activation. While CML patients had higher sP-selectin, AML patients had the highest state of hypercoagulability. Soluble P-selectin was correlated with leukocyte count and F1 + 2 in AML patients.

scholarly journals Soluble P-selectin and correlation with Prothrombin Fragment 1 + 2 in myeloid malignancies in Cipto Mangunkusumo general hospital

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Lugyanti Sukrisman
Keyword(s):  
Acute Myeloid Leukemia ◽  
Chronic Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Leukocyte Count ◽  
Endothelial Activation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Newly Diagnosed ◽  
Prothrombin Fragment ◽  
Soluble P ◽  
Acute Myeloid

Abstract Background Myeloid cells express microparticles that could increase the expression of adhesion molecules including P-selectin. We aimed to evaluate the level of soluble P-selectin (sP-selectin) and prothrombin fragment 1 + 2 (F1 + 2), and to determine correlation of sP-selectin with leukocyte count and F1 + 2 levels in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) patients. Methods Patients with newly diagnosed AML (n = 25), CML (n = 13), and controls (n = 17) were recruited in this study. The diagnosis of AML and CML is based on 2001 WHO and/or FAB criteria. Levels of sP-selectin and F1 + 2 were determined using enzyme-linked immunosorbent assay kits (Behring ELISA Processor-III® and Behring Enzygnost F1 + 2). Results sP-selectin was significantly elevated in CML patients compared to AML patients (p = 0.001). Levels of F1 + 2 in AML and CML patients were significantly increased in comparison to controls (p < 0.001 and p = 0.043). Levels of sP-selectin were significantly correlated to leukocyte count (r = 0.437; p = 0.029) and F1 + 2 (r = 0.436; p = 0.029) in AML patients. Conclusions AML and CML patients had an increased tendency to thrombosis. While CML patients had higher platelet and/or endothelial activation, hypercoagulable state are more pronounced in AML patients.

scholarly journals Clinico-hematological study of pancytopenia

1970 ◽  
Vol 2 (3) ◽  
pp. 207-210 ◽  
Author(s):  
A Lakhey ◽  
OP Talwar ◽  
VK Singh ◽  
Shiva Raj KC
Keyword(s):  
Bone Marrow ◽  
Teaching Hospital ◽  
Myeloid Leukemia ◽  
Hematological Malignancies ◽  
Hematological Malignancy ◽  
Complete Blood Count ◽  
Megaloblastic Anemia ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Erythroid Hyperplasia

Background: Pancytopenia refers to a reduction in all the three cellular elements of blood. The aim of this study was to identify the various causes of pancytopenia in patients attending to Manipal teaching hospital in Pokhara. Materials and Methods: This was a cross-sectional study, carried out in Manipal teaching hospital from August 2008 to August 2010. Fifty-four patients with pancytopenia were included in the study. Complete blood count, bone marrow aspirations and trephine biopsies were performed. Data were analyzed using SPSS 11.0 version. Results: Out of 54 cases, there were 16 cases (29.60%) of hypoplastic bone marrow, 15 cases (27.78%) of hematological malignancies, 13 cases (24.10%) of megaloblastic anemia, 4 cases (11.11%) of erythroid hyperplasia and 6 cases (7.41%) of normcellular bone marrow. Acute myeloid leukemia was the commonest hematological malignancy. Conclusion: The commonest cause of pancytopenia in our study was hypoplastic bone marrow followed by hematological malignancies and megaloblastic anemia. DOI: http://dx.doi.org/10.3126/jpn.v2i3.6023 JPN 2012; 2(3): 207-210

scholarly journals Prevalence of oral manifestations in patients with lupus erythematosus in a sample of the Egyptian population: a hospital based cross-sectional study

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 969
Author(s):  
Hager Moustafa Saeed ◽  
Eman Mohammad Amr ◽  
Alshaimaa Rezk Lotfy Rezk ◽  
Wesam Abd Elmoneim
Keyword(s):  
Oral Cavity ◽  
Lupus Erythematosus ◽  
Cross Sectional Study ◽  
Oral Lesions ◽  
Oral Manifestations ◽  
Sectional Study ◽  
Cross Sectional ◽  
Egyptian Population ◽  
Increased Risk ◽  
Risk Of Cancer

Background: Several systemic diseases manifest themselves in the oral cavity. Oral manifestations of lupus erythematosus (LE) are associated with a significantly increased risk of cancer. Dentists who are unaware of these lesions will possibly miss them. This cross-sectional study aimed to assess the prevalence of oral manifestations in patients with LE in a sample of the Egyptian population. Methods: A descriptive study was performed on 189 patients attending the Internal Medicine Department, Rheumatology Clinic in EL Qasr EL Ainy Hospital, Cairo University. Every patient was examined clinically after completing a questionnaire. Moreover, patients’ medical records were also evaluated.  The oral manifestations were recorded according to the WHO guide to physical examination of the oral cavity and classified according to their morphologic aspects and localization. Results: Out of 189 patients, there were 182 females (96.3%) and seven males (3.7%). The prevalence of oral lesions in LE patients was 55.6%. The most affected site was the tongue 25.7%. The most common clinical aspect was patches, 53%. About 77.1% of the lesions were asymptomatic. 74.3% of the patients had oral candidiasis. The prevalence of skin lesions in LE patients was 37.6%. The most common finding was malar rash 79%. Conclusions: The present study emphasizes the importance of early diagnosis of oral lesions to recognize patients with LE as the WHO considers oral manifestations of LE a widespread state associated with an increased risk of cancer. Also, implementation of oral hygiene measures and treatment to improve patients’ nutritional state and health-related quality of life are recommended.

Clinical Features, Complications, and Early Treatment Outcome of Pediatric Acute Myeloid Leukemia with Hyperleukocytosis.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4505-4505
Author(s):  
Hiroto Inaba ◽  
Ying Fan ◽  
Stanley Pounds ◽  
Jeffrey E. Rubnitz ◽  
Raul C. Ribeiro ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Clinical Features ◽  
Myeloid Leukemia ◽  
Leukocyte Count ◽  
Early Death ◽  
Pulmonary Complications ◽  
Early Mortality ◽  
Remission Induction ◽  
Increased Risk ◽  
Acute Myeloid

Abstract Patients with acute myeloid leukemia (AML) and hyperleukocytosis are at increased risk of early death from neurological and pulmonary complications due to leukostasis. Although leukapheresis is often used for rapid cytoreduction in these patients, a recent adult study showed no difference in the rate of early mortality between patients who underwent leukapheresis and historical controls. The scarcity of information about leukapheresis in childhood AML led us review our experience of 106 children with AML and hyperleukocytosis (i.e., initial leukocyte count ≥ 100 × 109/L) treated between 1968 and 2002. The presenting clinical features, early complications, and clinical outcomes during the first 2 weeks of remission induction therapy were analyzed according to two treatment eras: early (1968–1982) vs. recent (1983–2002), when leukapheresis was available. The entire cohort had a median age of 9.7 years (range, 0–19.9 years); initial leukocyte count of 161 × 109/L (100 to 1,600); hemoglobin concentration of 8.2 g/dL (2.9–15.4); and platelet count of 38.5 × 109/L (0 to 300). The presenting features were comparable between patients treated in the two eras with the exception of higher hemoglobin concentrations among those treated in the recent era (p=0.03). Platelet transfusion prior to chemotherapy was used more often in the recent era (p=0.001). Half of the cases (53 of 106) had FAB M4 or M5 subtypes. Twenty-one patients (19.8%) had grade 3 or 4 neurological, respiratory, and/or renal complications (according to NCI common criteria) at initial presentation, and 35 patients (33%) had one or more of these complications during the first 2 weeks after diagnosis (17 neurological; 20 respiratory; 16 renal). The frequency of the complications did not differ significantly between patients treated in the two eras. Patients with FAB M4/M5 AML were significantly more prone than others to respiratory (p=0.005) and renal (p=0.0002) complications during the first two weeks of therapy. Seventeen patients (16%) died during the first 2 weeks after diagnosis. The rate of early death was significantly higher in the early era than in the recent era (16/70 vs. 1/36, p=0.01). The time between admission and initiation of chemotherapy was significantly shorter (20.2 vs. 33.6 hours, p&lt;0.0001), and the reduction of leukocyte count before chemotherapy was significantly less (−3 × 109/L vs. −77.1 × 109/L, p&lt;0.0001) in patients treated in the early era, as compared to those from the recent era. Among patients treated in the recent era, the 20 who underwent leukapheresis had a higher initial leukocyte count (205.9 × 109/L vs. 115.5 × 109/L, p&lt;0.0001) than the 16 who did not undergo the procedure. Although the incidence of acute complications did not differ between the two eras, the rate of early death was markedly decreased in the recent era (from 23% to 2.8%), suggesting that leukapheresis/exchange transfusion might have prevented some early deaths. Our results suggest that improved supportive care, including the use of leukapheresis, decreases early mortality caused by leukostasis, especially in patients with M4 or M5 subtypes of AML.

scholarly journals Prevalence of Anemia and Associated Factors in Child Bearing Age Women in Riyadh, Saudi Arabia

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
AlJohara M. AlQuaiz ◽  
Ashry Gad Mohamed ◽  
Tawfik A. M. Khoja ◽  
Abdullah AlSharif ◽  
Shaffi Ahamed Shaikh ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Complete Blood Count ◽  
Deficiency Anemia ◽  
Cluster Sampling ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Cellular Analysis ◽  
Child Bearing ◽  
Increased Risk ◽  
Prevalence Of Anemia

Objective. To determine the prevalence and risk factors for anemia in child bearing age women in Riyadh, Saudi Arabia.Design. Cross-sectional survey was conducted using two-stage cluster sampling. 25 clusters (primary health care centers (PHCC)) were identified from all over Riyadh, and 45–50 households were randomly selected from each cluster. Eligible women were invited to PHCC for questionnaire filling, anthropometric measurements, and complete blood count. Blood hemoglobin was measured with Coulter Cellular Analysis System using light scatter method.Setting. PHCC.Subjects. 969 (68%) women out of 1429 women were included in the analysis.Results. Mean hemoglobin was 12.35 (±1.80) g/dL, 95% CI 12.24–12.46 with interquartile range of 1.9. Anemia (Hb <12 g/dL) was present in 40% (390) women. Mean (±SD) for MCH, MCV, MCHC, and RDW was 79.21 (±12.17) fL, 26.37 (±6.21) pg, 32.36 (±4.91) g/dL, and 14.84 (±4.65)%, respectively. Multivariate logistic regression revealed that having family history of iron deficiency anemia (OR 2.91, 95% CI 1.78–4.76) and infrequent intake of meat (OR 1.54, 95%CI 1.15–2.05) were associated with increased risk of anemia, whereas increasing body mass index (OR 0.95, 95% CI 0.92–0.97) was associated with reduced risk of anemia.Conclusion. Women should be educated about proper diet and reproductive issues in order to reduce the prevalence of anemia in Saudi Arabia.

scholarly journals Evaluation of brain CT-scan results and indications before lumbar puncture in children suspected of meningitis hospitalized in Ardabil city hospital

2018 ◽  
Vol 5 (11) ◽  
pp. 3585
Author(s):  
Majid Vafaie ◽  
Mehrdad Mirzarahimi ◽  
Afsaneh Enteshari-Moghaddam-moghaddam ◽  
Sahar Mousavi
Keyword(s):  
Ct Scan ◽  
Lumbar Puncture ◽  
Cellular Growth ◽  
Subdural Effusion ◽  
City Hospital ◽  
Cross Sectional ◽  
Brain Ct ◽  
Increased Risk ◽  
Brain Ct Scan ◽  
Risk Of Cancer

Background: Multiple use of CT scan is associated with an increased risk of cancer in the future especially in children due to cellular growth and mutation. The aim of this study was to evaluate the result and indications of brain CT scan before lumber puncture in children with suspected of meningitis.Methods: In this retrospective cross-sectional study, information of brain CT Scans on all meningitis suspected children under 12 years old hospitalized in Ardabil city hospital at 2016 who were candidates of CT Scan before Lumbar Puncture were extracted and the results were analyzed by statistical methods in SPSS version 22.Results: In this study, a total number of 67 patients were studied and CT scan changes were seen in 13 cases (19.4%) that of them 6 (46.2%) were male and rest of them were female. The mean age of the cases that had CT scan changes was 36.9±2.14 months and in other patients were 38.8±3.56 months. Of all patients, 13 patients experienced decrease in consciousness level that CT scan changes were observed in 8 cases (61.5%). Of the 13 patients (19.4%) had CT scan changes, 5 (38.4%) ha brain structural lesions.Conclusions: The prevalence rate of CT-scan changes was 19.4% and the most commonly detections in patients by CT scan, were structural disorder of the brain and subdural effusion.

scholarly journals Haematological profile of human immunodeficiency virus infected patients

2019 ◽  
Vol 5 (1) ◽  
pp. 164
Author(s):  
C. L. Chitra ◽  
R. Manipriya ◽  
K. Deepa
Keyword(s):  
Human Immunodeficiency Virus ◽  
Complete Blood Count ◽  
Stage Iv ◽  
Cd4 Count ◽  
Stage I ◽  
Cross Sectional ◽  
Number Of Patients ◽  
Increased Risk ◽  
Haematological Profile ◽  
Immunodeficiency Virus

<p class="abstract"><strong>Background:</strong> In India, approximately 6 million populations are affected by human immunodeficiency virus (HIV). Anemia and leukopenia, especially thrombocytopenia is seen commonly in HIV infections. Low CD4+ count and increased viral load are some of the factors associated with increased risk of thrombocytopenia. The aim of the study was to study the hematological changes that occur in HIV infected patients who attend the Institute of Venereology, before starting HAART.</p><p class="abstract"><strong>Methods:</strong> This cross-sectional study was conducted in the Institute of Venereology, Madras Medical College/Rajiv Gandhi Government General Hospital, Chennai in 100 treatment-naive HIV infected patients. Detailed history and clinical examination was done. Blood samples were collected. Complete blood count, CD4 count, prothrombin time, activated plasma thromboplastin time, peripheral smear etc., were done. Results were collected and analysed.<strong></strong></p><p class="abstract"><strong>Results:</strong> Out of 100 patients, 56% were males and 43% females and one transgender. Anaemia was detected in 72%patients. 73.5% males and 76.2% females with CD4 count &lt;350/μl were anemic. The commonest anaemia was normochromic normocytic, seen in 65% patients. 7 male and 7 female patients had leukopenia. 81.25%patients with lymphocytopenia had CD4 count &lt;350/μl. 12% males and 4% females had neutropenia. 17% had neutrophilia. Patients in WHO stage I did not have neutropenia. 23% patients had thrombocytopenia. 47% patients with thrombocytopenia were in stage IV. Maximum number of patients with normal platelet count was in stage I.</p><p class="abstract"><strong>Conclusions:</strong> Haematological abnormalities are a common occurrence during the course of HIV infection. Identifying and treating the haematological changes have great impact on both the morbidity and mortality of HIV infected patients.</p>

Thromboembolic risk in patients with high titre anticardiolipin and multiple antiphospholipid antibodies

2003 ◽  
Vol 90 (07) ◽  
pp. 108-115 ◽  
Author(s):  
Carolyn Neville ◽  
Joyce Rauch ◽  
Jeannine Kassis ◽  
Erika Chang ◽  
Lawrence Joseph ◽  
...  
Keyword(s):  
Risk Factors ◽  
Lupus Erythematosus ◽  
Complete Blood Count ◽  
Thrombotic Event ◽  
High Titre ◽  
Anticardiolipin Antibodies ◽  
Teaching Hospitals ◽  
Antiphospholipid Antibody ◽  
Cross Sectional ◽  
Increased Risk

SummaryAsymptomatic antiphospholipid antibody (aPL) carriers with high risk for thrombosis may benefit from preventive anticoagulation.It was our objective to test whether the risk of thrombosis increases with: 1) increasing titres of anticardiolipin antibodies (aCL) after adjustment for other cardiovascular risk factors and 2) the number of aPL detected.In a cross-sectional study, blood was collected from clinics in two teaching hospitals. The study included 208 individuals suspected of having an aPL and 208 age- and sex-matched controls having blood drawn for a complete blood count.Clinical variables included history of previous arterial (ATE) or venous (VTE) thrombotic events, traditional risk factors for cardiovascular disease, and systemic lupus erythematosus (SLE). Laboratory variables included IgG/IgM aCL, lupus anticoagulant, and IgG/IgM anti-β2-glycoprotein I.Mean age was 46.5 years and 83% were female. Seventy-five of the 416 participants had > 1 aPL, and 69 had confirmed > 1 ATE or VTE. Family history was positive in 48% of participants, smoking in 28%, hypertension in 16%, diabetes in 6%, and SLE in 20%. A 10-unit increase in aCL IgG titre was associated with an odds ratio (OR) [95% CI] of 1.07 [1.01-1.13] for ATE and 1.06 [1.02 - 1.11] for VTE. The odds of a previous thrombosis increased with each additional aPL detected: 1.5 [0.93-2.3] for ATE and 1.7 [1.1-2.5] for VTE.These results indicate that increased titres of aCL and multiple aPL were associated with an increased risk of a previous thrombotic event.

scholarly journals Prevalence of Microalbuminuria and associated factors among HIV−infected ART naïve patients at Mulago hospital, Kampala, Uganda.

2020 ◽  
Author(s):  
THOMAS KIGGUNDU ◽  
Pauline Byakika-Kibwika ◽  
Irene Andia Biraro ◽  
Gyaviira Makanga ◽  
Robert Kalyesubula
Keyword(s):  
Diabetes Mellitus ◽  
Associated Factors ◽  
Alcohol Intake ◽  
Complete Blood Count ◽  
Cd4 Count ◽  
P Value ◽  
Mulago Hospital ◽  
Cross Sectional ◽  
Increased Risk ◽  
The Mean

Abstract Background The kidney is one of the common target organs for HIV infection. Early detection of microalbuminuria, the earliest marker of renal damage is critical to slowing down progression to end stage renal disease if appropriate intervention is made. The burden of microalbuminuria and its associated factors in HIV−infected ART naive patients has not been determined in Uganda.Methods A cross-sectional study was conducted in the Mulago Immune suppression syndrome (ISS) clinic on adult HIV−infected ART naïve outpatients. Data was collected on age, sex, level of education, marital status, religion, address, and history of alcohol intake, diabetes mellitus, hypertension, medications and smoking. Measurement of blood pressure, weight and height to determine body mass index (BMI) and investigations including complete blood count (CBC),serum urea and creatinine, Liver function tests(LFTs), CD4+ count, spot morning urine albumin and urine creatinine to determine microalbuminuria were conducted. Logistic regression was used to estimate the strength of association between variables.ResultsA total of 185 adult participants were consecutively enrolled into the study. The mean (SD) age was 34.2(±9.0) years and majority (63.8%) were female. The mean (SD) CD4+ count 466±357 cells/µL, and BMI 23.1 (±4.9) kg/m 2 . The prevalence of microalbuminuria was 18.9%. None of the participants had albuminuria. CD4+ count <350cells/µL and BM1<18.5kg/m 2 were associated increased risk of microalbuminuria OR 3.8 (95%CI 1.7-8.3) (p value=0.01) and OR 4.7 (95%CI 1.82-12.4) (p value=0.03) respectively. Diabetes mellitus, hypertension, smoking, alcohol intake were not found to be significantly associated with microalbuminuria.Conclusion Microalbuminuria was highly prevalent in adult HIV−infected ART patients especially those with low CD4+ count and low BMI. There is need to study the effect of ART on microalbuminuria in adult HIV−infected patients. Key words: HIV, microalbuminuria, Uganda, ART naïve

scholarly journals Evaluation of Individual and Combined Markers of Urine Dipstick Parameters and Total Lymphocyte Count as a Substitute for CD4 Count in Low-Resource Communities in Ghana

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Enoch Odame Anto ◽  
Christian Obirikorang ◽  
Emmanuel Acheampong ◽  
Bright Amankwaa ◽  
Bright Oppong Afranie ◽  
...  
Keyword(s):  
Lymphocyte Count ◽  
Complete Blood Count ◽  
Total Lymphocyte Count ◽  
Cd4 Count ◽  
Cross Sectional ◽  
Low Resource ◽  
Urine Dipstick ◽  
Total Lymphocyte ◽  
Increased Risk ◽  
The Individual

We evaluated the individual and combined levels of urine dipstick and total lymphocyte count (TLC) as surrogate markers for CD4 count in a low-resource community in Ghana. This cross-sectional study recruited 200 HIV-infected patients from the Saint Francis Xavier Hospital, Assin Fosu, Ghana. Complete blood count, CD4 count, and urine dipstick analysis were measured for participants. The threshold values were determined as <350 cells/μl for CD4, <1200 cells/μl for TLC, and ≥+ on urine dipstick analysis. The mean age of participants was 43.09 years. Proteinuria ≥ + [aOR = 4.30 (3.0–18.5)], leukocyturia ≥ + [aOR = 2.91 (1.33–12.5)], hematuria ≥ + [aOR = 2.30 (1.08–9.64)], and TLC < 1200 cells/μl [aOR = 3.26 (3.94–15.29)] were significantly associated with increased risk of CD4 count < 350 cells/μl. Using the individual markers, the best substitute marker for predicting CD4 count < 350 cells/μl was proteinuria at a cutoff point ≥ 2++, AUC of 0.973, sensitivity of 97.6%, specificity of 100.0%, PPV of 100.0%, and NPV of 89.1%. A combination of ≤ 1200 TLC + ≥ 2++ (leukocyturia + proteinuria + hematuria) yielded an AUC of 0.980, sensitivity (72.8%), specificity (100.0%), PPV (100.0%), and NPV (97.9%). Proteinuria could serve as a noninvasive screening tool, but the combination of proteinuria, leukocyturia, hematuria, and TLC serves as a better substitute marker for CD4 count in monitoring the disease progression among HIV patients in low-resource communities.

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