scholarly journals Personalized metabolic profile by synergic use of NMR and HRMS

2021 ◽  
Author(s):  
Greta Petrella ◽  
Camilla Montesano ◽  
Sara Lentini ◽  
Giorgia Ciufolini ◽  
Domitilla Vanni ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Healthy Subjects ◽  
Metabolic Profile ◽  
Relative Concentration ◽  
Metabolite Identification ◽  
Chronic Cystitis ◽  
New Strategy ◽  
Absolute Concentrations ◽  
The Absolute ◽  
Analytical Standards

Abstract A new strategy that takes advantage of the synergism between NMR and LC-HRMS (SYNHMET), and that allows to obtain a unique list of the absolute concentrations of 164 metabolites in urine, is presented. Metabolite identification and quantification by this method in one of the most difficult biofluids to characterize, due to complexity and variability, is more accurate than what can be obtained using the two techniques separately. This result is achieved without the need for chemical reactions to cross-check the data between the two types of spectra, nor the use of analytical standards and calibration curves. The fact that the absolute rather than relative concentration is obtained allows the final dataset to be used to determine a patient's personalized profile. The number of quantifiable metabolites by the application of this method can be expanded in the future with further analysis. We will illustrate the use of SYNHMET in the study of urine samples from healthy subjects, patients with chronic cystitis and bladder cancer.

scholarly journals Personalized Metabolic Profile by Synergic Use of NMR and HRMS

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4167
Author(s):  
Greta Petrella ◽  
Camilla Montesano ◽  
Sara Lentini ◽  
Giorgia Ciufolini ◽  
Domitilla Vanni ◽  
...  
Keyword(s):  
Metabolic Profile ◽  
Missing Values ◽  
Metabolite Identification ◽  
Chronic Cystitis ◽  
Normal Ranges ◽  
New Strategy ◽  
Potential Applications ◽  
Minimum Number ◽  
Analytical Standards ◽  
Detailed Picture

A new strategy that takes advantage of the synergism between NMR and UHPLC–HRMS yields accurate concentrations of a high number of compounds in biofluids to delineate a personalized metabolic profile (SYNHMET). Metabolite identification and quantification by this method result in a higher accuracy compared to the use of the two techniques separately, even in urine, one of the most challenging biofluids to characterize due to its complexity and variability. We quantified a total of 165 metabolites in the urine of healthy subjects, patients with chronic cystitis, and patients with bladder cancer, with a minimum number of missing values. This result was achieved without the use of analytical standards and calibration curves. A patient’s personalized profile can be mapped out from the final dataset’s concentrations by comparing them with known normal ranges. This detailed picture has potential applications in clinical practice to monitor a patient’s health status and disease progression.

scholarly journals Differential sensitivities of bladder cancer cell lines to resveratol are unrelated to its metabolic profile

Oncotarget ◽  
2017 ◽  
Vol 8 (25) ◽  
pp. 40289-40304 ◽  
Author(s):  
Yang Yang ◽  
Chuangang Li ◽  
Hong Li ◽  
Moli Wu ◽  
Changle Ren ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Metabolic Profile ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell

[PP.05.28] RELATIONSHIP BETWEEN UROCORTIN 2 CONCENTRATION AND METABOLIC PROFILE IN HEALTHY SUBJECTS

2017 ◽  
Vol 35 ◽  
pp. e126-e127
Author(s):  
O. Siga ◽  
A. Dzieza-Grudnik ◽  
J. Walczewska ◽  
P. Wolkow ◽  
A. Borys ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Metabolic Profile

MP39-16 GENE THERAPY AGAINST BLADDER CANCER IN VIVO: A NEW STRATEGY TO FIGHT THE UROTHELIAL CARCINOMA

2014 ◽  
Vol 191 (4S) ◽  
Author(s):  
Eduardo Landerer ◽  
Maximiliano Bendek ◽  
Lorena Lobos ◽  
Miguel Ávila ◽  
Alexis Rivas ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bladder Cancer ◽  
Urothelial Carcinoma ◽  
New Strategy

scholarly journals T-cell activation and subset patterns are altered in B-CLL and correlate with the stage of the disease

Blood ◽  
1989 ◽  
Vol 74 (2) ◽  
pp. 786-792 ◽  
Author(s):  
TH Totterman ◽  
M Carlsson ◽  
B Simonsson ◽  
M Bengtsson ◽  
K Nilsson
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Healthy Subjects ◽  
Lymphocytic Leukemia ◽  
Stage Ii ◽  
T Cell Subsets ◽  
Cd8 Cells ◽  
Hla Dr ◽  
The Absolute

Abstract Two-color FACS analysis was used to study activated and “functional” T and natural killer (NK) cell subsets of circulating lymphocytes in 23 patients with B-type chronic lymphocytic leukemia (B-CLL) and in 30 healthy subjects. As compared with controls, B-CLL patients had increased absolute numbers of phenotypically activated, HLA-DR+ CD4+ and CD8+ cells and T suppressor/effector (CD11b+CD8+) cells. When patients in Rai stages II through IV (n = 11) were compared with cases in Rai stages O through I (n = 12), the former group of patients had higher numbers of activated CD4+ and CD8+ T cells and decreased levels of suppressor/effector T cells. The absolute numbers of T suppressor/inducer (CD45R+CD4+) cells were elevated in patients with stage O through I disease but within normal range in stage II through IV leukemia. We further showed that the absolute numbers of NK-like (CD16+) cells and their activated counterparts (DR+CD16+) are elevated in B-CLL patients as compared with healthy subjects. The comparison of relative T and NK subsets in the blood of patients and controls showed that the proportions of CD4+, CD8+, and CD16+ cells expressing the activation marker HLA-DR were increased in B-CLL. Furthermore, the percentage of T-suppressor/inducer (CD45R+) cells within the CD4+ population was decreased in the patients. The proportion of T- suppressor/effector (CD11b+) cells within the CD8+ subset was reduced in subjects with stage II-IV disease only. When stimulated in vitro with the T-cell-dependent inducer TPA, B-CLL cells from patients in Rai stages II through IV secreted larger amounts of IgM as compared with cells from stage O through I patients. A positive correlation was observed between the degree of phenotypic activation of CD4+ T-helper cells and their functional capacity to augment IgM secretion by autologous B-CLL cells. Our findings indicate a tumor cell-directed regulatory role of T cells (and possibly NK cells as well) in B-CLL. Furthermore, monitoring of phenotypically activated and functional T- cell subsets may be helpful in the prediction of disease progression and timing of therapy in B-CLL.

scholarly journals Pharmacokinetic profile and metabolite identification of bornyl caffeate and caffeic acid in rats by high performance liquid chromatography coupled with mass spectrometry

RSC Advances ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 4015-4027 ◽  
Author(s):  
Baimei Shi ◽  
Lingjian Yang ◽  
Tian Gao ◽  
Cuicui Ma ◽  
Qiannan Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Single Dose ◽  
Metabolic Profile ◽  
High Performance ◽  
Ion Trap ◽  
Metabolite Identification ◽  
Pharmacokinetic Profile ◽  
Tof Ms

We revealed the metabolic profile of bornyl caffeate by HPLC-Q-TOF/MS, and then simultaneously examined the pharmacokinetics of bornyl caffeate and CA after administration of a single dose of bornyl caffeate by HPLC ion trap MS.

scholarly journals LINC00467 Promotes Tumor Progression via Regulation of the NF-kb Signal Axis in Bladder Cancer

2021 ◽  
Vol 11 ◽  
Author(s):  
Jiawei Xiao ◽  
Lian Gong ◽  
Mengqing Xiao ◽  
Dong He ◽  
Liang Xiang ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Animal Experiments ◽  
Bioinformatic Analysis ◽  
New Strategy ◽  
Patient Prognosis

PurposeLong non-coding RNAs (lncRNAs) play an important role in the occurrence and development of bladder cancer, but the underlying molecular mechanisms remain largely unknown. In this study, we found that LINC00467 was significantly highly expressed in bladder cancer through bioinformatic analysis. The present study aimed to explore the role of LINC00467 in bladder cancer and its possible underlying molecular mechanisms.MethodsThe expression of LINC00467 was obtained from GEO (GSE31189), the TCGA database, and qRT-PCR. The role of LINC00467 in bladder cancer was assessed both in vitro and in vivo. RIP, RNA pulldown, and CO-IP were used to demonstrate the potential mechanism by which LINC00467 regulates the progression of bladder cancer.ResultsThrough the analysis of GEO (GSE133624) and the TCGA database, it was found that LINC00467 was highly expressed in bladder cancer tissues and that the expression of LINC00467 was significantly negatively correlated with patient prognosis. Cell and animal experiments suggest that LINC00467 promotes the proliferation and invasion of bladder cancer cells. On the one hand, LINC00467 can directly bind to NF-kb-p65 mRNA to stabilize its expression. On the other hand, LINC00467 can directly bind to NF-kb-p65 to promote its translocation into the nucleus to activate the NF-κB signaling pathway, which promotes the progression of bladder cancer.ConclusionsLINC00467 is highly expressed in bladder cancer and can promote the progression of bladder cancer by regulating the NF-κB signaling pathway. Therefore, targeting LINC00467 is very likely to provide a new strategy for the treatment of bladder cancer and for improving patient prognosis.

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