Production of Proline and Protease With Different Organic Wastes in Bacteria

2020 ◽  
Author(s):  
Hüseyin Kahraman ◽  
Cennet Canan Karaderi
Keyword(s):  
Environmental Impact ◽  
Protease Activity ◽  
Room Temperature ◽  
Organic Waste ◽  
Organic Wastes ◽  
Waste Materials ◽  
Protease Production ◽  
Modified Method ◽  
Cellular Debris ◽  
Phosphate Buffered Saline

Abstract Background: In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. Methods: 5 ml of different organic waste materials were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Results: Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using TCW, WCW and OWW organic waste. Conclusion: We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.

scholarly journals Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes)

2022 ◽  
Vol 82 ◽  
Author(s):  
H. Kahraman ◽  
C. C. Karaderi
Keyword(s):  
Waste Water ◽  
Organic Waste ◽  
Cheese Whey ◽  
Organic Wastes ◽  
Waste Materials ◽  
Protease Production ◽  
Waste Frying Oil ◽  
Modified Method ◽  
Cellular Debris ◽  
Olive Waste

Abstract In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.

scholarly journals EXPERIMENTAL INVESTIGATION OF BIODEGRADATION PROCESSES IN FOOD WASTE WITH DIFFERENT AMOUNTS OF MOISTURE

2006 ◽  
Vol 14 (4) ◽  
pp. 173-181 ◽  
Author(s):  
Pranas Baltrėnas ◽  
Audronė Jankaitė ◽  
Ervinas Raistenskis
Keyword(s):  
Experimental Investigation ◽  
Anaerobic Digestion ◽  
Environmental Impact ◽  
Organic Waste ◽  
Municipal Waste ◽  
Waste Materials ◽  
Waste Collection ◽  
Collection System ◽  
Temperature Changes ◽  
Negative Environmental Impact

The today's world faces a rapid increase of population and thus a growing amount of generated waste. Unsorted municipal waste is transported to landfills. The negative environmental impact of a landfill is related with generated odours, polluted leachate and waste itself. Most of present landfills do not meet minimal environmental and sanitary requirements, i e most of landfills do not have an installed leachate collection system. In Lithuania waste management is related with many problems. The municipal waste collection system is inefficient, and the equipment is outdated. Large amounts of recyclable waste materials mixed with other wastes are still disposed in landfills. Aiming to use anaerobic digestion technologies for organic waste and minimize their negative environmental impact, recyclables need to be separated. The paper gives information about experimental investigation with fruit, vegetable and meat waste humidified with addition of moisture to 75 %, 79 % and 85 %. The experiment lasted for 60 days, and the concentration of methane and oxygen as well as temperature changes in vessels were observed. The results showed that in 50 l containers of mixed waste set with moisture to 85 % higher amounts of methane were produced than in those humidified with less water.

Evaluation Indexes System for Assessing Road Construction with Recycled Waste Materials to Achieve Better Environmental Impacts

2011 ◽  
Vol 250-253 ◽  
pp. 1001-1006 ◽  
Author(s):  
De Zhen Chen ◽  
Cui Jie Geng ◽  
Wen Zhou Sun
Keyword(s):  
Energy Consumption ◽  
Environmental Impact ◽  
Waste Disposal ◽  
Large Scale ◽  
Construction Material ◽  
Bottom Ash ◽  
Road Construction ◽  
Waste Materials ◽  
Evaluation Indexes ◽  
Recycled Waste

Evaluation indexes system has been put forward in this paper for quantifying thesystematical energy consumption, resources consumption, total emissions’ change and waste disposal capacity in road construction with recycled waste materials involved. With help of this evaluation indexes system, the contributions to environmental improvement caused by recycling waste materials in road construction can be quantified through calculating savings on environmental impact potentials, savings on energy consumption, on virgin materials’ consumption and waste disposal capacity provided by road construction. Based on the construction project of a road section numbered No.20 EWK0+400 ~ EWK0+600 of North highway to Shanghai Pudong international airport, which was the first trial project of using several kinds of recycled waste materials including bottom ash from incinerators to replace commonly used materials such as gravel in large scale in road pavement, the results of the four indexes, namely, savings on energy consumption and virgin materials’ consumption, environmental impact potentials as well as waste disposal capacity were obtained. It was found out that with multi recycled waste materials replacing part of the common construction material, systematical energy consumption can be reduced by 30%, a large amount of virgin resource consumption can be avoid and road construction also provides a remarkable large “dumping site” for solid wastes; while at the same time environmental impact potentials were saved for most impact categories except for increase in Ecotoxicity, water chronic, which was caused by heavy metals’ leaching and can be prevented by pre-treatment. Those results are useful for guiding the utilization of recycled waste materials, as well as for developing new technology process and advanced materials in road construction.

Mitigating the Antimicrobial Activities of Selected Organic Acids and Commercial Sanitizers with Various Neutralizing Agents

2011 ◽  
Vol 74 (5) ◽  
pp. 820-825 ◽  
Author(s):  
YOEN JU PARK ◽  
JINRU CHEN
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Organic Acids ◽  
Shiga Toxin ◽  
Room Temperature ◽  
Sodium Thiosulfate ◽  
Antimicrobial Activities ◽  
Phosphate Buffered Saline ◽  
Lactic Acids

This study was conducted to evaluate the abilities of five neutralizing agents, Dey-Engley (DE) neutralizing broth (single or double strength), morpholinepropanesulfonic acid (MOPS) buffer, phosphate-buffered saline (PBS), and sodium thiosulfate buffer, in mitigating the activities of acetic or lactic acid (2%) and an alkaline or acidic sanitizer (a manufacturer-recommended concentration) againt the cells of Shiga toxin–producing Escherichia coli (STEC; n = 9). To evaluate the possible toxicity of the neutralizing agents to the STEC cells, each STEC strain was exposed to each of the neutralizing agents at room temperature for 10 min. Neutralizing efficacy was evaluated by placing each STEC strain in a mixture of sanitizer and neutralizer under the same conditions. The neutralizing agents had no detectable toxic effect on the STEC strains. PBS was least effective for neutralizing the activity of selected organic acids and sanitizers. Single-strength DE and sodium thiosulfate neutralized the activity of both acetic and lactic acids. MOPS buffer neutralized the activity of acetic acid and lactic acid against six and five STEC strains, respectively. All neutralizing agents, except double-strength DE broth, had a limited neutralizing effect on the activity of the commercial sanitizers used in the study. The double-strength DE broth effectively neutralized the activity of the two commercial sanitizers with no detectable toxic effects on STEC cells.

Production and characterisation of thermostable alkaline protease from Bacillus subtilis isolated from LA hot spring, Terengganu

2021 ◽  
Vol 16 (7) ◽  
pp. 84-91
Author(s):  
Maslinda Alias ◽  
Hakim Che Harun Mohammad ◽  
Ashraf Razali Nurul ◽  
Jasnizat Saidin ◽  
Nazaitulshila Rasit ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Alkaline Protease ◽  
Protease Activity ◽  
Hot Spring ◽  
Skim Milk ◽  
Industrial Applications ◽  
Protease Production ◽  
Significant Activity ◽  
Original Activity ◽  
Optimum Ph

This research aims to produce thermostable alkaline protease from Bacillus subtilis isolated from La Hot Spring, Terengganu, Malaysia. The study was also conducted to determine the optimum conditions for protease production and stability by considering several parameters including pH, temperature and salt concentration. All seven bacteria were screened on skim milk agar overnight at 37 °C. Three strains with the highest proteolytic activity were identified in protease specific medium. The thermostable alkaline protease had an optimum temperature of 60 °C which achieved 85.73, 82.90 and 83.05 U/mL of protease activity for the three strains respectively. Furthermore, the strains exhibited significant activity of more than 90% from their original activity. Meanwhile, the optimum pH for protease production was pH 9 with the protease activity of 76.76, 79.71 and 88.39 U/mL for TB4, TB6 and TB9 strains, respectively. Proteases were found stable at pH 9 where the loss did not exceed 30% of its original activity. Collectively, all of the data emphasised that proteases from B. subtilis were alkaline thermostable proteases in accordance with a recent report. The finding highlights the viability of the proteases for biotechnological and industrial applications.

High Pressure Treatment of Organic Wastes

1993 ◽  
Vol 28 (1) ◽  
pp. 243-248 ◽  
Author(s):  
J. Döllerer ◽  
P. A. Wilderer
Keyword(s):  
High Pressure ◽  
Room Temperature ◽  
Organic Materials ◽  
Waste Treatment ◽  
Organic Wastes ◽  
Pressure Treatment ◽  
Extreme Pressure ◽  
High Pressure Treatment ◽  
Denaturation Of Proteins

High pressure treatment of organic materials was introduced several years ago by Japanese researchers as a means to preserve food. Application potentials are also envisaged in the field of waste treatment. Hygienization effects, for instance, are achieved, when microbially contaminated samples are placed into an isostatic press and exposed, at room temperature, to a pressure of 4,000 bars and above. Deformation of the biopolymers under those extreme pressure conditions leads to effects such as coagulation, denaturation of proteins, crystallisation and/or gelatinization (Heremans 1992). As a result the microorganisms lose viability within a few minutes.

scholarly journals Sustainable Management of Organic Wastes in Sharjah, UAE through Co-Composting

2020 ◽  
Vol 3 (4) ◽  
pp. 76
Author(s):  
Md Maruf Mortula ◽  
Aqeel Ahmed ◽  
Kazi Parvez Fattah ◽  
Ghina Zannerni ◽  
Syed A. Shah ◽  
...  
Keyword(s):  
United Arab Emirates ◽  
Sustainable Management ◽  
Organic Waste ◽  
C:N Ratio ◽  
Nutrient Content ◽  
Organic Wastes ◽  
Long Run ◽  
Green Areas ◽  
High Nutrient ◽  
Minimal Treatment

Daily human activities and vast green areas produce substantial amounts of organic wastes that end up in landfills with minimal treatment. In addition to the problems associated with landfills, disposal through this method is unsustainable in the long run and does not allow recovering materials from the waste. This paper focuses on the co-composting of different organic wastes produced in The Emirate of Sharjah, United Arab Emirates (UAE), to optimize mixing proportions of three different kinds of wastes—sewage sludge (SS), green waste (GW), and food waste (FW). All three organic wastes were analyzed to determine their chemical composition and the mixing proportions. Ten different mixing proportions as a function of carbon:nitrogen (C:N ratios) were determined and mixed in a NatureMill composter. Compost samples were tested for pH, salinity, conductivity, moisture content, organic matter, organic carbon, phosphorus, total nitrogen, and final C:N ratio after 6 weeks. Results indicate that a period of 5–6 weeks is sufficient for the compost to stabilize. The varying mixing proportions produced a good-quality compost (C:N up to 20:1) with high nutrient content. The study reaffirms that co-composting can be a potential sustainable organic waste management option for the United Arab Emirates.

scholarly journals Comparative Study on the Environmental Impact of Traditional Clay Bricks Mixed with Organic Waste Using Life Cycle Analysis

2018 ◽  
Vol 10 (8) ◽  
pp. 2917 ◽  
Author(s):  
José Lozano-Miralles ◽  
Manuel Hermoso-Orzáez ◽  
Carmen Martínez-García ◽  
José Rojas-Sola
Keyword(s):  
Life Cycle ◽  
Environmental Impact ◽  
Comparative Study ◽  
Environmental Impacts ◽  
Life Cycle Analysis ◽  
Organic Waste ◽  
Organic Additives ◽  
Promising Alternative ◽  
Clay Bricks ◽  
The Impact

The construction industry is responsible for 40–45% of primary energy consumption in Europe. Therefore, it is essential to find new materials with a lower environmental impact to achieve sustainable buildings. The objective of this study was to carry out the life cycle analysis (LCA) to evaluate the environmental impacts of baked clay bricks incorporating organic waste. The scope of this comparative study of LCA covers cradle to gate and involves the extraction of clay and organic waste from the brick, transport, crushing, modelling, drying and cooking. Local sustainability within a circular economy strategy is used as a laboratory test. The energy used during the cooking process of the bricks modified with organic waste, the gas emission concentrate and the emission factors are quantified experimentally in the laboratory. Potential environmental impacts are analysed and compared using the ReCiPe midpoint LCA method using SimaPro 8.0.5.13. These results achieved from this method are compared with those obtained with a second method—Impact 2002+ v2.12. The results of LCA show that the incorporation of organic waste in bricks is favourable from an environmental point of view and is a promising alternative approach in terms of environmental impacts, as it leads to a decrease of 15–20% in all the impact categories studied. Therefore, the suitability of the use of organic additives in clay bricks was confirmed, as this addition was shown to improve their efficiency and sustainability, thus reducing the environmental impact.

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