scholarly journals Effects of Lead Exposure on Blood Electrical Impedance Spectroscopy of Mice

2021 ◽  
Author(s):  
Binying Yang ◽  
Jia Xu ◽  
Shao Hu ◽  
Boning You ◽  
Qing Ma
Keyword(s):  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Lead Exposure ◽  
Electrical Impedance ◽  
Equivalent Circuit Model ◽  
Impedance Spectrum ◽  
Nyquist Plot ◽  
Electrical Impedance Spectroscopy ◽  
Impedance Method ◽  
Range Data

Abstract Background: Lead is a nonessential heavy metal, which can inhibit heme synthesis and has significant cytotoxic effects. Nevertheless, its effect on the electrical properties of red blood cells (RBCs) remains unclear. Consequently, this study aimed to investigate the electrical properties and the electrophysiological mechanism of lead exposure in mouse blood using Electrical Impedance Spectroscopy (EIS). Methods: AC impedance method was used to measure the electrical impedance of healthy and lead exposure blood of mice in 0.01-100 MHz frequency range. Data characteristic of the impedance spectrum, Bodes plot, Nyquist plot and Nichols plot, and three elements equivalent circuit model were used to explicitly analyze the differences in amplitude-frequency, phase-frequency, and the frequency characteristic of blood in electrical impedance properties. Results: Compared with the healthy blood in control mice, the changes in blood exposed to lead was as follows: (I) the hematocrit decreased; (II) the amplitude-frequency and phase-frequency characteristics of electrical impedance decreased; (III) the characteristic frequencies ( f 0 ) were significantly increased; (IV) the electrical impedance of plasma, erythrocyte membrane, and hemoglobin decreased, while the conductivity increased. Conclusion: Therefore, EIS can be used as an effective method to monitor blood and RBCs abnormalities caused by lead-exposure.

scholarly journals Effects of lead exposure on blood electrical impedance spectroscopy of mice

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Binying Yang ◽  
Jia Xu ◽  
Shao Hu ◽  
Boning You ◽  
Qing Ma
Keyword(s):  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Lead Exposure ◽  
Electrical Impedance ◽  
Equivalent Circuit Model ◽  
Impedance Spectrum ◽  
Nyquist Plot ◽  
Frequency Characteristics ◽  
Electrical Impedance Spectroscopy ◽  
Range Data

Abstract Background Lead is a nonessential heavy metal, which can inhibit heme synthesis and has significant cytotoxic effects. Nevertheless, its effect on the electrical properties of red blood cells (RBCs) remains unclear. Consequently, this study aimed to investigate the electrical properties and the electrophysiological mechanism of lead exposure in mouse blood using Electrical Impedance Spectroscopy (EIS) in 0.01–100 MHz frequency range. Data characteristic of the impedance spectrum, Bodes plot, Nyquist plot and Nichols plot, and Constant Phase Element (CPE) equivalent circuit model were used to explicitly analyze the differences in amplitude–frequency, phase–frequency, and the frequency characteristics of blood in electrical impedance properties. Results Compared with the healthy blood in control mice, the changes in blood exposed to lead were as follows: (i) the hematocrit decreased; (ii) the amplitude–frequency and phase–frequency characteristics of electrical impedance decreased; (iii) the characteristic frequencies (f0) were significantly increased; (iv) the electrical impedance of plasma, erythrocyte membrane, and hemoglobin decreased, while the conductivity increased. (v) The pseudo-capacitance of cell membrane (CPE_Tm) and the intracellular pseudo-capacitance (CPE-Ti) were decreased. Conclusions Therefore, EIS can be used as an effective method to monitor blood and RBC abnormalities caused by lead exposure. The electrical properties of the cells can be applied as an important observation in the evaluation of the toxic effects of heavy metals.

scholarly journals Electrical Impedance Spectroscopy as Electrical Biopsy for Monitoring Radiation Sequelae of Intestine in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Pei-Ju Chao ◽  
Eng-Yen Huang ◽  
Kuo-Sheng Cheng ◽  
Yu-Jie Huang
Keyword(s):  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Integrated Circuits ◽  
Electrical Impedance ◽  
Roc Analysis ◽  
Electrical Impedance Spectroscopy ◽  
Electrical Characteristics ◽  
Injury Score ◽  
Histological Changes ◽  
Radiation Sequelae

Electrical impedance is one of the most frequently used parameters for characterizing material properties. The resistive and capacitive characteristics of tissue may be revealed by electrical impedance spectroscopy (EIS) as electrical biopsy. This technique could be used to monitor the sequelae after irradiation. In this study, rat intestinal tissues after irradiation were assessed by EIS system based on commercially available integrated circuits. The EIS results were fitted to a resistor-capacitor circuit model to determine the electrical properties of the tissue. The variations in the electrical characteristics of the tissue were compared to radiation injury score (RIS) by morphological and histological findings. The electrical properties, based on receiver operation curve (ROC) analysis, strongly reflected the histological changes with excellent diagnosis performance. The results of this study suggest that electrical biopsy reflects histological changes after irradiation. This approach may significantly augment the evaluation of tissue after irradiation. It could provide rapid results for decision making in monitoring radiation sequelae prospectively.

Current Source Design for Electrical Bioimpedance Spectroscopy

2008 ◽  
pp. 359-367 ◽  
Author(s):  
Fernando Seoane ◽  
Ramón Bragos ◽  
Kaj Lindecrantz ◽  
Pere Riu
Keyword(s):  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Biological Tissue ◽  
Electrical Impedance ◽  
Current Source ◽  
Electrical Impedance Spectroscopy ◽  
Bioimpedance Spectroscopy ◽  
Electrical Bioimpedance ◽  
Electronic Instrumentation ◽  
Passive Electrical Properties

The passive electrical properties of biological tissue have been studied since the 1920s, and with time, the use of Electrical Bioimpedance (EBI) in medicine has successfully spread (Schwan, 1999). Since the electrical properties of tissue are frequency-dependent (Schwan, 1957), observations of the bioimpedance spectrum have created the discipline of Electrical Impedance Spectroscopy (EIS), a discipline that has experienced a development closely related to the progress of electronic instrumentation and the dissemination of EBI technology through medicine.

scholarly journals Electrical impedance spectroscopy in relation to seed viability and moisture content in snap bean (Phaseolus vulgaris L.)

2002 ◽  
Vol 12 (1) ◽  
pp. 17-29 ◽  
Author(s):  
T. Repo ◽  
D.H. Paine ◽  
A.G. Taylor
Keyword(s):  
Phaseolus Vulgaris ◽  
Impedance Spectroscopy ◽  
Moisture Content ◽  
Electrical Impedance ◽  
Seed Viability ◽  
Impedance Spectrum ◽  
Electrical Circuit ◽  
Electrical Impedance Spectroscopy ◽  
Snap Bean ◽  
Viable Seeds

A method, electrical impedance spectroscopy (EIS), is introduced to study seed viability non-destructively. Snap bean (Phaseolus vulgaris L.) seeds were studied by EIS to determine the most sensitive EIS parameter(s) and the optimal range of moisture content (MC) for separation of viable and non-viable seeds. Hydrated seeds exhibited two impedance arcs in the complex plane at the frequency range from 60 Hz to 8 MHz, and impedance spectra of viable and non-viable seeds differed. The hydrated seeds were best-modelled by an equivalent electrical circuit with two distributed circuit elements in series with a resistor (Voigt model). Moisture content and seed viability had strong effects on the EIS parameters. The most sensitive EIS parameters for detecting the differences between viable and non-viable seeds were the capacitance log(C2), the resistance R2, the resistance ratio R2/R1 and the apex ratio, which all represent specific features of the impedance spectrum. The highest differentiation in the EIS parameters between the viable and non-viable seeds occurred in partially imbibed seeds between MC of 40 and 45% (fresh weight basis).

Role of phase angle measurement in electrical impedance spectroscopy

2013 ◽  
Vol 27 (4) ◽  
pp. 377-383 ◽  
Author(s):  
I. Cseresnyés ◽  
K. Rajkai ◽  
E. Vozáry
Keyword(s):  
Energy Dissipation ◽  
Impedance Spectroscopy ◽  
Phase Angle ◽  
Characteristic Frequency ◽  
Electrical Impedance ◽  
Angle Measurement ◽  
Electrical Impedance Spectroscopy ◽  
Impedance Method ◽  
Soil System ◽  
Current Frequency

Abstract Importance of phase angle measurement during the application of electrical impedance spectroscopy was studied by executing pot experiments with maize. Electrical impedance, phase angle (strength of capacitive character), and dissipation factor in the plant-soil system were scanned between 100 and 10 000 Hz current frequency. The frequency-dependent change in the phase angle could be described by optimum curves culminating within 920-3 650 Hz. Since the rate of energy dissipation is independent of root extent, the higher phase angle and lower energy dissipation were associated with the higher coefficient of determination achieved for the root electrical impedance - root system size (root dry mass and root surface area) regressions. The characteristic frequency selected on the basis of phase angle spectra provided a higher significance level at statistical comparison of plant groups subjected to stress conditions influencing root development. Due to the physicochemical changes observable in aging root tissue, the apex of phase angle spectra, thus the characteristic frequency, shifted continuously toward the higher frequencies over time. Consequently, the regularly repeated phase angle measurement is advisable in time-course studies for effective application of the electrical impedance method, and the systematic operation at the same frequency without determination of phase angle spectra should be avoided.

scholarly journals Experimental Study of Electrical Properties of Pharmaceutical Materials by Electrical Impedance Spectroscopy

2020 ◽  
Vol 10 (18) ◽  
pp. 6576
Author(s):  
Manuel Vázquez-Nambo ◽  
José-Antonio Gutiérrez-Gnecchi ◽  
Enrique Reyes-Archundia ◽  
Wuqiang Yang ◽  
Marco-A. Rodriguez-Frias ◽  
...  
Keyword(s):  
System Identification ◽  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Electrical Impedance ◽  
Wide Frequency Range ◽  
Electrical Impedance Spectroscopy ◽  
Frequency Range ◽  
Pharmaceutical Materials ◽  
Identification Techniques

The physicochemical characterization of pharmaceutical materials is essential for drug discovery, development and evaluation, and for understanding and predicting their interaction with physiological systems. Amongst many measurement techniques for spectroscopic characterization of pharmaceutical materials, Electrical Impedance Spectroscopy (EIS) is powerful as it can be used to model the electrical properties of pure substances and compounds in correlation with specific chemical composition. In particular, the accurate measurement of specific properties of drugs is important for evaluating physiological interaction. The electrochemical modelling of compounds is usually carried out using spectral impedance data over a wide frequency range, to fit a predetermined model of an equivalent electrochemical cell. This paper presents experimental results by EIS analysis of four drug formulations (trimethoprim/sulfamethoxazole C14H18N4O3-C10H11N3O3, ambroxol C13H18Br2N2O.HCl, metamizole sodium C13H16N3NaO4S, and ranitidine C13H22N4O3S.HCl). A wide frequency range from 20 Hz to 30 MHz is used to evaluate system identification techniques using EIS data and to obtain process models. The results suggest that arrays of linear R-C models derived using system identification techniques in the frequency domain can be used to identify different compounds.

scholarly journals Temperature Dependent Electrical Properties and Catalytic Activities ofLa2O3–CO2–H2OPhase System

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
Bahcine Bakiz ◽  
Frédéric Guinneton ◽  
Madjid Arab ◽  
Sylvie Villain ◽  
Abdeljalil Benlhachemi ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Thermal Decomposition ◽  
Electrical Properties ◽  
Impedance Spectroscopy ◽  
Electrical Impedance ◽  
Catalytic Properties ◽  
Electrical Impedance Spectroscopy ◽  
Temperature Dependent ◽  
Catalytic Activities ◽  
Coprecipitation Route

We present a study of electrical properties and catalytic activities of materials belonging to the hydrated carbonated systemLa2O3–CO2–H2O. The polycrystalline hydroxycarbonate, dioxycarbonate, and oxide are prepared via a coprecipitation route followed by heat treatment. The electrical conduction of the phases obtained by thermal decomposition fromLaOHCO3,H2Ois analyzed by electrical impedance spectroscopy, from25°Cto950°C, under air. The catalytic properties ofLaOHCO3,La2O2CO3andLa2O3polycrystalline phases are studied by FTIR spectroscopy, in presence of gas mixtures CO-air andCH4-air, at temperatures ranging between100°Cto525°C. The three materials behave differently in presence of CO orCH4gases.

scholarly journals Dielectrophoretic and Electrical Impedance Differentiation of Cancerous Cells Based on Biophysical Phenotype

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 401
Author(s):  
Ina Turcan ◽  
Iuliana Caras ◽  
Thomas Gabriel Schreiner ◽  
Catalin Tucureanu ◽  
Aurora Salageanu ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Tumor Cells ◽  
Cancer Cell ◽  
Primary Tumor ◽  
Electrical Impedance ◽  
Mononuclear Cells ◽  
Equivalent Circuit Model ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Peripheral Blood Mononuclear

Here, we reported a study on the detection and electrical characterization of both cancer cell line and primary tumor cells. Dielectrophoresis (DEP) and electrical impedance spectroscopy (EIS) were jointly employed to enable the rapid and label-free differentiation of various cancer cells from normal ones. The primary tumor cells that were collected from two colorectal cancer patients and cancer cell lines (SW-403, Jurkat, and THP-1), and healthy peripheral blood mononuclear cells (PBMCs) were trapped first at the level of interdigitated microelectrodes with the help of dielectrophoresis. Correlation of the cells dielectric characteristics that was obtained via electrical impedance spectroscopy (EIS) allowed evident differentiation of the various types of cell. The differentiations were assigned to a “dielectric phenotype” based on their crossover frequencies. Finally, Randles equivalent circuit model was employed for highlighting the differences with regard to a series group of charge transport resistance and constant phase element for cancerous and normal cells.

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