Multiple Mechanisms Synergistically Induce Pseudomonas Aeruginosa Multiple Drug Resistance
Abstract Background: This study was designed to detect the molecular epidemiological characteristics and resistant mechanism of carbapenem resistant Pseudomonas aeruginosa (CRPA) which provide reference for the prevention and treatment of hospital CRPA infection. Methods: 34 strains of CRPA from 2018 to 2019 were isolated and their resistance to 13 commonly used antibiotics was detected using TDR-300B Plus VitEK-2 compact automatic bacterial identification instrument. Then carbapenemase production was detected using Carbe NP test. The efflux pumps MexA and outer membrane protein OprD proteins were detected using RT-PCR and class Ⅰ integron carried with drug-resistant genes were detected using PCR and sequences analysis. Results: Among 34 strains of CRPA, 22 strains were multiple drug resistance (MDR) and 5 strains were extensively drug-resistant (XDR). The results of class Ⅰ integron carried drug-resistant gene sequencing analysis showed the class Ⅰ integron mainly carried aminoglycoside or quinolone antibacterial drug resistant genes. Conclusion: Multiple mechanisms play an important role in the formation and development of MDR or XDR resistance.