The Effect of Different Concentration of Aqueous Extracts of Labisia pumila in Preventing Osteoporosis and Improvement of Dermal Elasticity in Polycystic Ovary Syndrome Rats

Abstract Background: Labisia pumila (LP) a Malaysian herb thought to have phytoestrogenic effects in rats with PCOS. In this study we investigate the effects of LP aqueous extracts on bone composition, bone biomarkers and metabolic disorder in female rats fed continuously with high fat diet to induce PCOS. Hypothesis: The administration of Labisia pumila treatment in PCOS rats for 90 days will ameliorate the adverse effect of osteoporosis by reducing the inflammatory cytokine and improving the dermal elasticity of PCOS rats. Study design: On the 90th day of the development of PCOS model rats fed with high fat diet and after the vaginal smear analysis indicating a prolonged estrus cycle of more than 2 weeks, all PCOS rats were divided into 4 groups which consisted of control, placebo (water), LP25 (LP 25mg/kg) and LP50 (LP 50 mg/kg) respectively. All PCOS continue to received the formulated high fat diet and control animals continued to received normal chow and water ad libitum. Method: Vehicle and treatments, which were given orally by using stomach gavaging needle size 16 gauge straight and curve retrolingual administration. The rats were sacrificed at the end of the trial phase and organs, tissues and blood samples were harvested for multiple assays and analysis listed below.Results: The level of estradiol was significantly increased in LP25 and LP50 as compared with placebo. The inflammatory cytokine of C-reactive protein and TNF-ɑ were significantly decreased in LP25 and LP50.Conclusion: Treatment of LP extract might reduce the inflammatory cytokine related to the formation of osteoporosis and loss of bone mass, increase the insulin sensitivity and reduce of osteoporosis in PCOS patients. The phytoestrogenic of LP all of the above significant positive results are proven in lowering osteoporosis and metabolic disorder in PCOS rats.

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High-fat diet exposure from pre-pubertal age induces polycystic ovary syndrome (PCOS) in rats

Reproduction ◽

10.1530/rep-17-0584 ◽

2018 ◽

Vol 155 (2) ◽

pp. 139-149 ◽

Cited By ~ 14

Author(s):

Roshni Patel ◽

Gaurang Shah

Keyword(s):

High Fat Diet ◽

Cell Layer ◽

Polycystic Ovary ◽

Receptor Expression ◽

Female Rats ◽

Histopathological Changes ◽

High Fat ◽

Ovary Syndrome ◽

Lh Receptor ◽

Group I

Polycystic ovary syndrome (PCOS) is associated with hyperandrogenism, oligo-anovulation, polycystic ovaries and metabolic syndrome. Many researchers reported that PCOS often starts with menarche in adolescents. Presently available animal model focuses on ovarian but not metabolic features of PCOS. Therefore, we hypothesized that high-fat diet feeding to pre-pubertal female rats results in both reproductive and metabolic features of PCOS. Pre-pubertal female rats were divided into two groups: group I received normal pellet diet and group II received high-fat diet (HFD). In the letrozole study, adult female rats were divided into two groups: group I received 1% carboxy methyl cellulose and group II received 1 mg/kg letrozole orally. Oral glucose tolerance test, lipid profile, fasting glucose, insulin, estrus cycle, hormonal profile, ovary weight, luteinizing hormone (LH) receptor and follicle-stimulating hormone receptor expression were measured. Polycystic ovarian morphology was assessed through histopathological changes of ovary. Feeding of HFD gradually increase glucose intolerance and fasting insulin levels. Triglyceride level was higher in HFD study while total cholesterol level was higher in the letrozole study. Alteration in testosterone and estrogen levels was observed in both studies. LH receptor expression was upregulated only in HFD study. Histopathological changes like increase cystic follicle, diminished granulosa cell layer and thickened theca cell layer were observed in letrozole as well as HFD study. High-fat diet initiated at pre-puberty age in rats produces both metabolic disturbances and ovarian changes similar to that observed clinically in PCOS patients. Letrozole on the other hand induces change in ovarian structure and function.

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Obesity induced by high-fat diet promotes insulin resistance in the ovary

Journal of Endocrinology ◽

10.1677/joe-09-0461 ◽

2010 ◽

Vol 206 (1) ◽

pp. 65-74 ◽

Cited By ~ 56

Author(s):

Eliana H Akamine ◽

Anderson C Marçal ◽

João Paulo Camporez ◽

Mara S Hoshida ◽

Luciana C Caperuto ◽

...

Keyword(s):

Insulin Resistance ◽

Signaling Pathway ◽

Insulin Signaling ◽

High Fat Diet ◽

Ovarian Function ◽

Polycystic Ovary ◽

Insulin Signaling Pathway ◽

Female Rats ◽

High Fat ◽

Obese Rats

Besides the effects on peripheral energy homeostasis, insulin also has an important role in ovarian function. Obesity has a negative effect on fertility, and may play a role in the development of the polycystic ovary syndrome in susceptible women. Since insulin resistance in the ovary could contribute to the impairment of reproductive function in obese women, we evaluated insulin signaling in the ovary of high-fat diet-induced obese rats. Female Wistar rats were submitted to a high-fat diet for 120 or 180 days, and the insulin signaling pathway in the ovary was evaluated by immunoprecipitation and immunoblotting. At the end of the diet period, we observed insulin resistance, hyperinsulinemia, an increase in progesterone serum levels, an extended estrus cycle, and altered ovarian morphology in obese female rats. Moreover, in female obese rats treated for 120 days with the high-fat diet, the increase in progesterone levels occurred together with enhancement of LH levels. The ovary from high-fat-fed female rats showed a reduction in the insulin receptor substrate/phosphatidylinositol 3-kinase/AKT intracellular pathway, associated with an increase in FOXO3a, IL1B, and TNFα protein expression. These changes in the insulin signaling pathway may have a role in the infertile state associated with obesity.

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High fat diet-induced estrus cycle disruption: effects of Ficus asperifolia

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2014-0074 ◽

2015 ◽

Vol 12 (3) ◽

Cited By ~ 7

Author(s):

Esther Ngadjui ◽

Pepin Alango Nkeng-Efouet ◽

Telesphore Benoit Nguelefack ◽

Albert Kamanyi ◽

Pierre Watcho

Keyword(s):

Body Weight ◽

High Fat Diet ◽

Body Weight Gain ◽

Tween 80 ◽

The Body ◽

Female Rats ◽

Vaginal Smear ◽

High Fat ◽

Methanolic Extracts ◽

Obese Rats

Abstract:: Obesity was reached by feeding female rats with a HFD for 10 weeks. Vaginal smear was observed daily for 3 weeks after animals were obese. Then, 70 animals with abnormal estrus cyclicity were selected and partitioned into two sets of 35 animals. Each set was further divided into seven groups of five rats. These obese rats with disrupted estrus cyclicity were orally administered the aqueous and methanolic extracts (100 and 500 mg/kg), distilled water (10 mL/kg), 5% Tween 80 (10 mL/kg) or lutenyl (0.8 µg/kg) once a day for 1 week (set I) or 4 weeks (set II). Estrus cyclicity, body weight gain, hematocrit, lipid profile, ovarian, uterine and hepatic growth indices were determined at the end of each treatment.: HFD increased the body weight of the animals by 27% and disrupted the estrus cyclicity by 98.44%. Aqueous extract (100 mg/kg) of: Our data support the anecdotal claims of

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Faculty Opinions recommendation of High-fat diet induces significant metabolic disorders in a mouse model of polycystic ovary syndrome.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718523489.793499307 ◽

2014 ◽

Author(s):

Virendra Mahesh

Keyword(s):

Polycystic Ovary Syndrome ◽

Mouse Model ◽

High Fat Diet ◽

Metabolic Disorders ◽

Polycystic Ovary ◽

High Fat ◽

Ovary Syndrome

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Acute effect of high-intensity interval training exercise on redox status in the ovaries of rats fed a high-fat diet

Reproduction Fertility and Development ◽

10.1071/rd20326 ◽

2021 ◽

Vol 33 (12) ◽

pp. 713

Author(s):

Rodrigo L. Furtado ◽

Jonathan Elias R. Martins ◽

Maria Alice F. Oliveira ◽

Denise D. Guerreiro ◽

Naiza A. R. de Sá ◽

...

Keyword(s):

High Intensity ◽

High Fat Diet ◽

Interval Training ◽

Redox Status ◽

Female Rats ◽

Standard Diet ◽

High Intensity Interval Training ◽

High Fat ◽

Antral Follicles ◽

High Intensity Interval

This study demonstrates the effect of a single high-intensity interval training (HIIT) session on the redox status of rat ovaries with excess adiposity. Forty Wistar female rats (mean (±s.e.m.) weight 94.40 ± 13.40 g) were divided into two groups and fed either a standard diet (SD) or a high-fat diet (HFD) for 62 days. At the end of this period, the rats were subjected to a single HIIT session and were killed 24 h after exercise. Both groups subjected to exercise (SDex and HFDex) generated a significantly higher antioxidant environment by presenting a higher thiol content, which represents a lower oxidation rate of GSH than their respective controls (SD and HFD). The percentage of morphologically normal primary follicles decreased, whereas that of antral follicles increased, in the SDex group. In addition, the HFD group had a higher percentage of degenerated antral follicles than the SD and SDex groups. Cells immunoreactive for α-smooth muscle actin were seen in the cortical stroma and thecal layer enclosing late secondary and tertiary follicles in all groups. Moreover, heme oxygenase and cytochrome P450 family 19 subfamily A member 1 (Cyp19A1) labelling was seen in all antral follicles. Progesterone concentrations were significantly higher in the HFDex than SDex group. In conclusion, this study indicates that a single session of HIIT may result in an improvement in ovary redox status because of metabolic muscle activity by inducing physiological adaptation after exercise in a paracrine manner.

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