Evaluation of Fruit Extracts Influence on the Susceptibility of Escherichia Coli Rods to the Bactericidal Action of Human Serum

2021 ◽  
Author(s):  
Agnieszka Cisowska ◽  
Janina Gabrielska
Keyword(s):  
Escherichia Coli ◽  
Human Serum ◽  
Mechanisms Of Action ◽  
Bactericidal Action ◽  
E Coli ◽  
Fruit Extracts ◽  
Extract Concentration ◽  
Japanese Quince ◽  
Normal Human ◽  
Dog Rose

Abstract This study determined the influence of the methanol (ME) and water (WE) fruit extracts obtained from eight species of Rosaceae and Grossulariacae family on the susceptibility of Escherichia coli rods to the lytic action of normal human serum (NHS). Bacteria were incubated for 24 h in tryptic soy broth with varying concentrations (1, 5, 10, 20, 30, 40, and 50 mg ml-1) of raspberry, cherry, hawthorn, dog rose, gooseberry, chokeberry, quince, and Japanese quince extracts and then the bactericidal activity of NHS was established. We found that the resistance of E. coli rods to the bactericidal action of serum was altered by prior incubation with all tested extracts and was dependent on plant extract concentration. Among the tested extracts, gooseberry (both ME and WE), raspberry ME and cherry WE were responsible for the most profound changes in serum resistance of E. coli rods. Evaluation of the antimicrobial mechanisms of action of phenolics-rich plant extracts has the potential to impact the development of novel compounds with promising applications in food and biopharmaceutical industry or medical approaches to preventing and treating pathogenic infections.

scholarly journals Sequential metabolic expressions of the lethal process in human serum-treated Escherichia coli: role of lysozyme

1980 ◽  
Vol 28 (3) ◽  
pp. 735-745
Author(s):  
R J Martinez ◽  
S F Carroll
Keyword(s):  
Escherichia Coli ◽  
Human Serum ◽  
Membrane Integrity ◽  
Response To Treatment ◽  
Gram Negative Bacteria ◽  
Human Lysozyme ◽  
E Coli ◽  
Normal Human ◽  
Hydrolysis Of

Several metabolic parameters indicative of Escherichia coli function and integrity were kinetically examined in response to treatment with normal human serum in the presence and absence of functional human lysozyme. Specific inhibition of this enzyme in bacteriolytic and bactericidal reactions was accomplished by using purified rabbit anti-human lysozyme immunoglobulin G. Initiation of the complement-mediated alterations of cytoplasmic membrane integrity, as judged by the leakage of 86Rb from prelabeled cells or the hydrolysis of o-nitrophenyl-beta-D-galactopyranoside by a cryptic strain, was found to be independent of lysozyme action. Furthermore, inhibition of macromolecular synthesis by E. coli in response to serum treatment occurred at the same time regardless of the functional state of lysozyme. Although the rate and extent of bacteriolysis were reduced in the absence of lysozyme, the bactericidal kinetics was unaffected. These results demonstrate that the lethal events associated with the action of antibody and complement on gram-negative bacteria are independent of lysozyme, suggesting an accessory role for this enzyme in immune reactions. A possible temporal sequence of complement-induced effects occurring at the cell surface is presented.

scholarly journals High-density transposon libraries utilising outward-oriented promoters identify mechanisms of action and resistance to antimicrobials

2020 ◽  
Vol 367 (22) ◽  
Author(s):  
Chris Coward ◽  
Gopujara Dharmalingham ◽  
Omar Abdulle ◽  
Tim Avis ◽  
Stephan Beisken ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Pathogenic Bacteria ◽  
Selective Advantage ◽  
Mechanisms Of Action ◽  
Over Expression ◽  
E Coli ◽  
Synthase Inhibitor ◽  
Established Technique ◽  
Bacterial Transposon

ABSTRACT The use of bacterial transposon mutant libraries in phenotypic screens is a well-established technique for determining which genes are essential or advantageous for growth in conditions of interest. Standard, inactivating, transposon libraries cannot give direct information about genes whose over-expression gives a selective advantage. We report the development of a system wherein outward-oriented promoters are included in mini-transposons, generation of transposon mutant libraries in Escherichia coli and Pseudomonas aeruginosa and their use to probe genes important for growth under selection with the antimicrobial fosfomycin, and a recently-developed leucyl-tRNA synthase inhibitor. In addition to the identification of known mechanisms of action and resistance, we identify the carbon–phosphorous lyase complex as a potential resistance liability for fosfomycin in E. coli and P. aeruginosa. The use of this technology can facilitate the development of novel mechanism-of-action antimicrobials that are urgently required to combat the increasing threat worldwide from antimicrobial-resistant pathogenic bacteria.

scholarly journals Moringa oleifera Seeds Extract Activity on Enteropathogenic Escherichia coli and Aeromonas hydrophyla Cells in Aquatic Microcosm

2019 ◽  
Vol 7 (2) ◽  
pp. 13 ◽  
Author(s):  
Claire Stephane Metsopkeng ◽  
Chretien Lontsi Djimeli ◽  
Olive Vivien Noah Ewoti ◽  
Lucienne Marlyse Moungang ◽  
Paul Alain Nana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Moringa Oleifera ◽  
Potable Water ◽  
Incubation Temperature ◽  
Enteropathogenic Escherichia Coli ◽  
Incubation Condition ◽  
E Coli ◽  
Extract Concentration ◽  
Cell Incubation ◽  
Potential Exploitation

This study aimed to evaluate in microcosm condition, the survival of Aeromonas hydrophila and Enteropathogenic Escherichia coli (EPEC), in the presence of M. oleifera aqueous seeds extract at concentrations varying from 1 to 40 g/L, and under 4 °C and 23 °C incubation temperature. It has been noted that cell abundances decrease gradually with the increasing in the seeds extract concentration. However, a marked cells regrowth was sometimes noted. In monospecies cell incubation condition, under 4 °C, the EPEC cells inhibition percentages (CIP) values varied from 52.12 to 99.84%. Those of A. hydrophila varied from 13.2 to 96%. The lowest CIPs were noted at the extract concentration 1g/L for EPEC and A. hydrophila. The highest CIP value was registered at 10 and 40 g/L for EPEC and at 15 g/L for A. hydrophila. Under 23 °C incubation, the EPEC CIPs values varied from 74.04 to 99.9% and those of A. hydrophila varied from 21.2 to 97.8%. For E. coli, the lowest and the highest CIP were recorded at the extract concentration 1g/L and 30 g/L, respectively. In bispecies cells incubation condition, the CIPs were relatively different. These results show the potential exploitation of M. oleifera extracts in the microbiological treatment of potable water.

Susceptibility of Gram-negative bacteria to the synergistic bactericidal action of serum and polymyxin B nonapeptide

1986 ◽  
Vol 32 (1) ◽  
pp. 66-69 ◽  
Author(s):  
Petri Viljanen ◽  
Helena Käyhty ◽  
Martti Vaara ◽  
Timo Vaara
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Enterobacter Cloacae ◽  
Polymyxin B ◽  
Gram Negative Bacteria ◽  
Bactericidal Action ◽  
Gram Negative ◽  
Degree Of Sensitization ◽  
Normal Human ◽  
Klebsiella Spp

Polymyxin B nonapeptide was able to sensitize Escherichia coli strains and strains of Salmonella typhimurium, Klebsiella spp., Enterobacter cloacae, Pseudomonas aeruginosa, and Haemophilus influenzae to the bactericidal action of fresh normal human serum. The degree of sensitization varied significantly within the strains. Strains of Proteus mirabilis, Neisseria gonorrhoeae, and N. meningitidis remained resistant.

scholarly journals Dynamic Mechanisms of the Bactericidal Action of an Al2O3-TiO2-Ag Granular Material on an Escherichia coli Strain

2015 ◽  
Vol 81 (20) ◽  
pp. 7135-7142 ◽  
Author(s):  
Marie-Anne Tartanson ◽  
Laurence Soussan ◽  
Matthieu Rivallin ◽  
Sophie Pecastaings ◽  
Cristian V. Chis ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Granular Material ◽  
Morphological Changes ◽  
Membrane Damage ◽  
Coli Strain ◽  
Cell Integrity ◽  
Bactericidal Action ◽  
Intact Cells ◽  
Content Type ◽  
E Coli

ABSTRACTThe bactericidal activity of an Al2O3-TiO2-Ag granular material against anEscherichia colistrain was confirmed by a culture-based method. In particular, 100% of microorganisms were permanently inactivated in 30 to 45 min. The present work aimed to investigate the mechanisms of the bactericidal action of this material and their dynamics onEscherichia coliusing different techniques. Observations by transmission electron microscopy (TEM) at different times of disinfection revealed morphological changes in the bacteria as soon as they were put in contact with the material. Notably highlighted were cell membrane damage; cytoplasm detachment; formation of vacuoles, possibly due to DNA condensation, in association with regions exhibiting different levels of electron density; and membrane lysis. PCR and flow cytometry analyses were used to confirm and quantify the observations of cell integrity. The direct exposure of cells to silver, combined with the oxidative stress induced by the reactive oxygen species (ROS) generated, was identified to be responsible for these morphological alterations. From the first 5 min of treatment with the Al2O3-TiO2-Ag material, 98% ofE. coliisolates were lysed. From 30 min, cell viability decreased to reach total inactivation, although approximately 1% of permeableE. colicells and 1% of intact cells (105genomic units · ml−1) were evidenced. This study demonstrates that the bactericidal effect of the material results from a synergic action of desorbed and supported silver. Supported silver was shown to generate the ROS evidenced.

scholarly journals IMMUNITY REACTIONS OF HUMAN SUBJECTS TO STRAINS OF PNEUMOCOCCI OTHER THAN TYPES I, II AND III

1933 ◽  
Vol 57 (1) ◽  
pp. 95-109 ◽  
Author(s):  
Maxwell Finland ◽  
W. D. Sutliff
Keyword(s):  
Human Serum ◽  
Human Blood ◽  
Human Subjects ◽  
Protective Action ◽  
Group Iv ◽  
Bactericidal Action ◽  
Blood Samples ◽  
Viii And Ix ◽  
Normal Human ◽  
Normal Human Blood

1. A group of 72 human subjects were studied with respect to the immune reactions of their blood and sera to Types I, II and III pneumococci and to 4 other types (V, VI, VIII and IX) previously included in Group IV. 2. The same general relationships were observed for all of these types as were previously demonstrated for Types I, II and III. Each type was specific in relation to the bactericidal action of normal human blood and the protective action of normal human serum. 3. The frequency with which pneumococcidal action for any pair of types was present for both or absent for both in the same blood samples was slightly greater than that calculated from the frequencies with which each of the types was killed separately. 4. No closer correlation could be demonstrated between the reaction of the blood of these subjects to Types II and V or between Types III and VIII pneumococci, types related in their reaction with artificially prepared immune sera, than was observed between unrelated strains.

scholarly journals UJI EFEKTIFITAS ANTIBAKTERI EKSTRAK KULIT BATANG JAMBU MONYET (Bellucia pentamera Naudin) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Salmonella typhi

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Farid Priandi ◽  
Fathul Yusro ◽  
Farah Diba ◽  
Yeni Mariani ◽  
. Nurhaida
Keyword(s):  
Escherichia Coli ◽  
Typhoid Fever ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Negative Effects ◽  
E Coli ◽  
Extract Concentration ◽  
The People ◽  
The Media

People still use plants as a medicine to overcome a disease, because it is easily to obtain and relatively does not provide significant negative effects. One of the plants utilized by the people is Bellucia pentamera Naudin (B. pentamera). Diarrhea and typhoid fever are diseases that often assault Indonesian people. The bacterias were used in this study were Escherichia coli (E. coli) and Salmonella typhi (S. typhi). This study aimed to analyze the antibacterial effectiveness of B. pentamera bark extracts against E. coli and S. typhi, by Kirby-Bauer diffusion method with PCA as the media. The highest extract concentration against E. coli is 400 mg/ml possess an inhibition of 20±1 mm, and towards S. typhi is 80 mg/ml which possess an inhibition of 24.67±0,58 mm.Keywords: Bellucia pentamera, Disc Diffussion, Efectiveness, Escherichia coli, Salmonella typhi.

Sign in / Sign up

Export Citation Format

Share Document