scholarly journals Optimizing the Fermentation Conditions and Determining the Disease Control and Growth Promotion Function of Bacillus Amyloliquefaciens 3-5

2021 ◽  
Author(s):  
Ting Ma ◽  
Chengde Yang ◽  
Fengfeng Cai ◽  
Lingxiao Cui ◽  
Zhezhe Li ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Corn Starch ◽  
Growth Promotion ◽  
Plant Diseases ◽  
Rice Hull ◽  
Control Effect ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Initial Ph ◽  
Solids Content

Abstract Bacillus spp. are helping to develop towards sustainable agriculture and have become a research hotspot in the field of plant pathology because they have great development potential such as control fungal plant diseases. Bacillus Amyloliquefaciens 3-5 was used as antagonistic bacteria in this experiment. The optimal medium for solid-state fermentation of B. amyloliquefaciens 3-5 contained bran (35%), rice hull powder (40%), corn gluten (20%), bean flour (15%), corn starch (1.5%), beef extract (2.5%) and MgSO4 (1.5%), and the optimal fermentation conditions included an inoculum of 6%, a solids content of 36 g/L, a feed-to-water ratio of 1:1, a fermentation temperature of 32 ℃, an initial pH of 7.0 and fermentation time of 44 h. When the dosage of the B. amyloliquefaciens 3-5 agent was 10%, the thick stems, root length and plant height of cucumber were significantly higher than those of the control (P＜0.05), and the growth rates were 77.45 %, 35.77 %, and 53.33 %, respectively in pot experiments. Compared with the control, and the preventive effect and therapeutic effect on cucumber Fusarium wilt were 72.09 % and 48.83 % by the application of B. amyloliquefaciens 3-5 agent，which showed that cucumber Fusarium wilt was successfully controlled by a newly isolated strain of B. Amyloliquefaciens 3-5. These results suggested that the prevention and control effect of B. amyloliquefaciens 3-5 agent on cucumber Fusarium wilt could not be underestimated.

Study on the Optimization of Fermentation Conditions for Actinomycete F10

2011 ◽  
Vol 480-481 ◽  
pp. 148-152
Author(s):  
Lei Yang ◽  
Guo Ying Zhou ◽  
Guang Tao Song
Keyword(s):  
Antimicrobial Activity ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Fermentation Time ◽  
Nutritional Factors ◽  
Fermentation Conditions ◽  
Antimicrobial Substances ◽  
Initial Ph ◽  
Optimization Of Fermentation ◽  
Fermentation Media

In order to improve the yield of antimicrobial substances extracted from actinomycetes F10 strains fermentation, antimicrobial activity and cell dry weight were taken as the main indicators, we studied the effects on F10 strain’s biological activity of the different fermentation media, different carbon, nitrogen and other nutrients factor, and the initial pH and other non-nutritional factors. The results showed that the optimum fermentation medium is: yeast extract 0.5%, glucose 1%, KH2PO4 0.1%, NaCl 0.1%, CaCO30.3%, pH natural. Strain F10 optimal fermentation conditions were: initial pH 7.0, fermentation time 168h.

Study on the Liquid Fermentation to Produce Monascus Pigment with Corn Starch and Antibacteria

2011 ◽  
Vol 183-185 ◽  
pp. 1336-1340 ◽  
Author(s):  
Wei Xu
Keyword(s):  
Bacillus Subtilis ◽  
Corn Starch ◽  
Raw Materials ◽  
Orthogonal Experiment ◽  
Liquid Volume ◽  
Fermentation Condition ◽  
Corn Flour ◽  
Fermentation Time ◽  
Monascus Pigments ◽  
Initial Ph

Corn flour was used as raw materials, Monascus M3428 was selected. Monascus pigment was produced by liquid-state fermentation. Through the orthogonal experiment to study the influence of the liquid volume of flasks, the table rotation，the fermentation time and the fermentation fluid initial pH to Monascus pigments production. The optimal fermentation condition was obtained: 100 mL fermentation fluid in 500 mL flasks; rotational speed of 150r/min; fermentation time of 14d; fermentation fluid initial pH of 4.5. Further preliminarily study the inhibition function against E.coli, Bacillus subtilis, White aureus, Yeast and Mycete. The results was obtained: Monascus pigments had strong inhibition function against Bacillus subtilis, had weak inhibition function against E.coli and Yeast, but had no inhibition function against Staphylococcus albus or Mycete.

scholarly journals Preparation and Antioxidant Activity of Purple Potato Wine

2015 ◽  
Vol 9 (1) ◽  
pp. 282-287 ◽  
Author(s):  
Liu Zhong-hua ◽  
Guo Jie
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Anion ◽  
Sugar Content ◽  
Raw Material ◽  
Wine Production ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Fermentation Temperature ◽  
Initial Ph ◽  
Yeast Inoculum

Purple potatoes were used as raw material to study the purple potato wine production process and antioxidant activity. This paper analyzed different fermentation time, fermentation temperature, yeast inoculum, initial pH, the initial sugar content on alcohol and anthocyanin contents of purple potato wine by single factor experiments and response surface methodology(RSM). The results showed that the optimum fermentation conditions of purple potato wine were as follows: fermentation temperature was 26oC, yeast inoculum was 0.15%, fermentation time was 7 d, initial pH was 3.0 and initial sugar content was 11 %. Under these conditions the alcohol and anthocyanin contents of purple potato wine could reach 10.55%/Vol and 6.42 μg/mL, respectively. The purple potato wine was purple, bright in colour, pleasant fragrance and pure taste. Prepared purple potato wine had the ability of reducing Fe3+ and scavenging superoxide anion radicals, which meant that purple potato wine had certain antioxidant activity.

Study on the Metabolites of a Bacteria Strain Producing Esterifying Synthetase

2012 ◽  
Vol 550-553 ◽  
pp. 1080-1084
Author(s):  
Hui Bo Luo ◽  
Chun Hui Wei ◽  
Xian Ling Yuan
Keyword(s):  
Acetic Acid ◽  
Identification System ◽  
Higher Alcohols ◽  
Automated Identification ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Geobacillus Thermoglucosidasius ◽  
Fermentation Temperature ◽  
Initial Ph

The metabolites characteristics of a bacteria producing esterifying synthetase in different fermentation conditions were studied. A bacteria strain producing esterifying enzyme was isolated from Luzhou-flavor Daqu and it was identified by Biolog Microbes automated identification System. Fermentation conditions such as fermentation time, fermentation initial pH and fermentation temperature were changed. The fermentatiom broth was extracted by ethanol and analyzed by GC-MS. The results showed that the strain was Geobacillus thermoglucosidasius. With the prolonging of fermentation time, methanol and various higher alcohols were gradually decreased and even disappeared, but the esters were gradually increased. With the rise of fermentation initial pH, the acids were decreased. When initial pH was 7.5, more esters were produced, but some metabolites were not changed with the initial pH of fermentation, such as acetic acid, acetaldehyde, 3-methyl butanol and 3-Hydroxy-2-butanone etc. With the increase of fermentation temperature, the alcohols and acids kinds increased. When the fermentation temperature was 35°C, the more esters were produced.

Optimization of Fermentation Conditions for Blue Pigment Production by Bacterial Strain T2013

2014 ◽  
Vol 1073-1076 ◽  
pp. 286-291
Author(s):  
Yong Zuo ◽  
Peng Jiang ◽  
Xiao Long Wang ◽  
Bin Fu ◽  
Xiao Long Yang ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Orthogonal Test ◽  
Blue Pigment ◽  
Liquid Volume ◽  
Initial Value ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Inoculum Volume ◽  
Optimization Of Fermentation ◽  
Single Factor Experiment

The fermentation conditions of Bacterial Strain T2013 were studied. The fermentation conditions of T2013 were investigated with single-factor experiment. In addition to, the fermentation conditions were optimized through the orthogonal test. The results showed the highest production of blue pigment was obtained on condition that fermentation time was 2d, the liquid volume was 50ml in the 250ml shake-flask, the inoculum volume was 2%, the initial value of pH was 7.6, fermentation temperature was 37°C and the speed was 150r/min.The fermentation conditions of Bacterial Strain T2013 were preliminarily determined,whichprovides a theoretical basis for the industrial production of blue pigment.

Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3

2008 ◽  
Vol 13 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Kang-Ik Jo ◽  
You-Jung Lee ◽  
Bo-Kyung Kim ◽  
Bo-Hwa Lee ◽  
Chung-Han Chung ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Pilot Scale ◽  
Rice Hull ◽  
Scale Production ◽  
Pilot Scale Production

Optimization of the Fermentation Conditions for 1-Deoxynojirimycin Production by Streptomyces lawendulae Applying the Response Surface Methodology

2011 ◽  
Vol 7 (3) ◽  
Author(s):  
Zhao-Jun Wei ◽  
Le-Chun Zhou ◽  
Hua Chen ◽  
Gui-Hai Chen
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Starch Content ◽  
Ultra Violet ◽  
Soluble Starch ◽  
High Yield ◽  
Yield Strain ◽  
Fermentation Time ◽  
Ethyl Sulfate ◽  
Initial Ph

Moranoline (1-Deoxynojirimycin, DNJ) is a piperidine alkaloid, and shows high inhibit activities to glucoamylase and ?-glucosidase. One DNJ high-yield strain of Streptomyces lawendulae was obtained after isolated form soil and mutated with the ultra violet (UV) and ethyl sulfate (DES), which named as TB-412, and can produce DNJ with 35.925 mg/L. Response surface methodology (RSM) was applied to optimize the parameters of DNJ yield from S. lawendulae TB-412. The effects of independent variables of fermentation, including time, temperature, initial pH and the soluble starch content were investigated. The statistical analysis showed that the fermentation time, pH and the soluble starch content, and the quadratics of time, temperature, pH and the soluble starch content, as well as the interactions between fermentation time and pH, and time and the soluble starch content, showed significant effects on DNJ yield. The optimal process parameters for DNJ production within the experimental range of the variables researched was at 11d, 27 °C, pH 7.5, and 8% soluble starch content. At this condition, the DNJ yield was predicted to be 42.875 mg/L.

scholarly journals Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

REAKTOR ◽  
2017 ◽  
Vol 5 (2) ◽  
pp. 79
Author(s):  
Abdullah Abdullah ◽  
H. B. Mat
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Raw Material ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Pineapple Waste ◽  
Fermentation Liquid ◽  
Initial Ph

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

scholarly journals Evaluation of fermented tea (kombucha) quality

2020 ◽  
Vol 26 (4) ◽  
Author(s):  
Vanesa Šliažaitė ◽  
Aurelija Paulauskienė ◽  
Aurimas Krasauskas
Keyword(s):  
Antioxidant Activity ◽  
Acetic Acid ◽  
Sensory Evaluation ◽  
Total Content ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Soluble Solids Content ◽  
Fermentation Conditions ◽  
Solids Content

The research was done at Aleksandras Stulginskis University (Vytautas Magnus University Agriculture Academy since 2019) in 2017–2018. The research object was kombucha beverage brewed from Camellia sinensis L. black, green, oolong, pu-erh tea and fermented for 9 and 22 days by microorganisms at different room lighting conditions. The soluble solids content, active acidity and titratable acidity were determined in teas fermented for 22 days. The total amount of phenolics compounds, antioxidant activity, yeast colonies and sensory evaluation of tea were determined in teas fermented for 9 days. The research results showed that the quality of fermented tea was dependent on the tea species, but fermentation conditions were not essential. During the fermentation the soluble solids content of tea decreased by an average of 0.60% and the content of acetic acid increased by 0.34%. The highest content of acetic acid was found on the 22nd day of fermentation in all species of teas. The active acidity (pH) of all investigated species of teas decreased significantly during the fermentation process. The total content of phenolic compunds in all species of teas during the fermentation increased from 20 to 57% regardless of fermentation conditions. The highest content of total phenolic compounds was established in teas fermented for 9 days. The antioxidant activity of teas fermented for 9 days varied depending on the fermentation conditions. For black and green teas fermented in the light the antioxidant activity decreased, for oolong and pu-erh teas fermented in the dark the antioxidant activity increased. The number of yeast colonies increased in all tea samples during the fermentation. The best taste was fixed for pu-erh tea, which scored the highest (11.4) in sensory evaluation.

scholarly journals Optimization of some fermentation conditions for bioethanol production from microalgae using response surface method

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Samar A. El-Mekkawi ◽  
Sayeda M. Abdo ◽  
Farag A. Samhan ◽  
Gamila H. Ali
Keyword(s):  
Algal Biomass ◽  
Treatment Plant ◽  
Pilot Scale ◽  
Simultaneous Optimization ◽  
High Rate ◽  
Bioethanol Production ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Promising Source ◽  
Bioethanol Yield

Abstract Background Algal biomass fermentation is one of the promising alternatives for bioethanol production. The bioethanol yield relies on fermentation conditions as the algal biomass amount, the yeast volume (% v/v), and the fermentation time. In this work, algal biomass harvested from a pilot-scale high rate algal pond (HRAP) was fermented anaerobically using immobilized Saccharomyces cerevisiae (ATCC 4126). The HRAP was constructed at the Zenin wastewater treatment plant (WTP), Giza, Egypt. A separate hydrolysis fermentation process (SHF) was applied for algal biomass. The effect of the algal biomass amount, the yeast volume (% v/v), and the time of fermentation as three independent variables were studied simultaneously and analyzed statistically using Design-Expert software V6.0.8. Results The harvested algal biomass from HRAP contains 45% carbohydrates and was dominated by Microcystis sp. The results revealed that optimum bioethanol yield 18.57 g/L is achieved by fermenting 98.7 g/L algae using 15.09% of the volume immobilized yeast for 43.6 h with a 95% confidence interval. Conclusion Microalgae grown on wastewater are a promising source of bioethanol production. Maximizing the ethanol production is achieved by optimizing the fermentation parameters as algal biomass, fermentation time, and yeast volume percent. The simultaneous optimization of the parameters using a statistical program is an effective way to maximize the production and predict a model that describes the relationship between these parameters and their response. The prospective research is going to study the effect of these predicted parameters on continuous fermentation on the semi-pilot scale.

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