scholarly journals Risk of leakage with a new tissue containment system for power morcellation during gynecologic laparoscopy: An in vitro study

2021 ◽  
Author(s):  
Fang Zhao ◽  
Jing Liang ◽  
Bin Ling
Keyword(s):  
Indigo Carmine ◽  
In Vitro Study ◽  
Control Group ◽  
Minimally Invasive Surgical ◽  
Removal Time ◽  
Containment System ◽  
Power Morcellation ◽  
Gynecologic Laparoscopy ◽  
Experimental Group

Abstract Purpose To evaluate leakage and tissue dissemination associated with a new tissue containment system for tissue removal during laparoscopic myomectomy morcellation using rigid pipes that can be seamlessly connected to detachable trocars. Methods Pork specimens were stained with indigo carmine dye and morcellated under laparoscopic guidance in a plastic trainer box. Morcellation was performed using two different containment systems. First, a polyurethane bag, 12 mm sheath for introduction into the peritoneal cavity, and 11 mm optic sleeve (control group). Second, a new tissue containment system using rigid pipes and detachable trocars (experimental group). All bags were inflated to 14–20 mmHg pressure using a standard CO2 insufflator. Visual evidence of spilled tissue or dye and procedure times were recorded. Results Thirty trials were performed using a multi-port approach and the two tissue containment systems. The leakage rate was 0.03% for the experimental group and 26.6% for the control group (p < 0.005). Morcellation time was significantly shorter in the experimental group compared to that in the control group (p < 0.05). Mean bag introduction and removal times of the experimental group were shorter than those of the control group; however, the removal time differences were not statistically significant. Conclusion The current study quantified leakage during morcellation and the convenience provided by a new tissue containment system. Further studies and clinical trials are needed to corroborate the findings and to evaluate the use of the new tissue containment system for minimally invasive surgical treatment of tumors.

scholarly journals Cortical Piezo-Puncture as a Minimally Invasive Method for Reducing MiniScrew Implant Insertion Torque: A Preliminary in vitro Study

2018 ◽  
Vol 8 ◽  
pp. 124-130
Author(s):  
Juan Pablo Gomez ◽  
Lisbetd Giraldo ◽  
Daniela Montoya ◽  
Miguel Urrea
Keyword(s):  
In Vitro Study ◽  
Control Group ◽  
Insertion Torque ◽  
Miniscrew Implants ◽  
Mini Implants ◽  
Intact Bone ◽  
Invasive Method ◽  
Miniscrew Implant ◽  
Experimental Group

Objective The objective of this study was to determine the effect of cortical piezo-puncture (CPP) on maximum insertion torque (MIT), maximum removal torque (MRT), and maximum axial load (MAL) during the insertion of self-drilling miniscrew implants (MSI), in an experimental model with proximal epiphysis of bovine tibia. Materials and Methods A comparative study was conducted using two groups of 20 self-drilling MSI inserted in intact bone (control group) and in bone with previous CPP (experimental group). MIT, MRT, and MAL of the 20 mini implants of each group were measured. Using SPSS software, Student’s t-test was applied to compare MIT and MRT and the U-test Mann–Whitney test was applied to compare MAL in both groups as well as Pearson and Spearman correlation. Results In the experimental group, average values of 12.85 (±4,32) Newton x centimeters (Ncm), 13.7 (±4,54) Ncm, and 22,474 (±895,95) gF for MIT, MRT, and MAL were found, respectively. In the control group, average values found for MIT, MRT, and MAL were 20.2 (±4,7) Ncm, 22.3 (±5,17) Ncm, and 4688,7 (±320,18) gF, respectively. Statistically significant differences were observed in MIT, MRT, and MAL between control and experimental groups (P < 0,001). Conclusions CPP before insertion of orthodontic MSI in bovine tibia significantly reduces MIT, MRT, and MAL.

Does Addition of Propolis to Glass Ionomer Cement Alter its Physicomechanical Properties? An in Vitro Study

2016 ◽  
Vol 40 (5) ◽  
pp. 400-403 ◽  
Author(s):  
P Subramaniam ◽  
KL Girish Babu ◽  
G Neeraja ◽  
S Pillai
Keyword(s):  
Compressive Strength ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Physicomechanical Properties ◽  
Control Group ◽  
Glass Ionomer ◽  
Experimental Group ◽  
Ionomer Cement

Propolis is a natural resinous substance produced by honey bees. The antimicrobial effects of glass ionomer cement have been shown to improve with the addition of propolis; however its effect on the physicomechanical properties of the cement is not known. Aim: The purpose of this study was to evaluate the compressive strength and solubility of conventional restorative glass ionomer cement following the addition of propolis. Study design: Twenty half cylindrical samples were prepared with conventional restorative glass ionomer cement formed the control group. Another twenty samples were prepared with propolis added to conventional restorative glass ionomer cement formed the experimental group. The compressive strength was assessed using universal testing machine. To assess solubility, the samples were immersed in deionised water at room temperature, for 7 days. The solubility was measured as a difference in the weight of the sample; prior to immersion and following immersion at the end of each day. Results: The control group had a significantly higher mean compressive strength of 146.26 Mpa as compared to the experimental group (135.06 Mpa). The solubility between the groups was significant. Conclusion: In comparison to the control group, incorporation of propolis to conventional restorative glass ionomer cement decreased the compressive strength significantly. The solubility of the cement in the experimental group increased significantly over 7day period as compared to the control group.

scholarly journals Influence of Thymoquinone Exposure on the Micro-Hardness of Dental Enamel: An In Vitro Study

2019 ◽  
Vol 13 (03) ◽  
pp. 318-322 ◽  
Author(s):  
Imran Farooq ◽  
Saqib Ali ◽  
Intisar Ahmad Siddiqui ◽  
Khalifa S. Al-Khalifa ◽  
Mohammed Al-Hariri
Keyword(s):  
Artificial Saliva ◽  
Dental Enamel ◽  
In Vitro Study ◽  
Rank Test ◽  
Control Group ◽  
Micro Hardness ◽  
Signed Rank Test ◽  
The Mean ◽  
Experimental Group

Abstract Objective The aim of this study was to assess changes in micro-hardness level of enamel after it was exposed to thymoquinone (TQ). Materials and Methods Sixteen enamel blocks were prepared and divided into two groups (each group received eight blocks, n = 8); Gp 1 (control): enamel blocks kept in 100 mL artificial saliva (AS) for 24 hours and Gp 2: enamel blocks kept in a mixture of TQ powder (1 g) and AS (100 mL) for 24 hours. Post-immersion they were subjected to simulated brushing with each sample receiving 8,000 linear strokes. For brushing, 3 mL of AS and TQ oil was used for groups 1 and 2, respectively. Enamel surfaces were analyzed for changes in values of surface micro-hardness (pre-immersion, post-immersion, and post-brushing) by obtaining Vickers hardness number (VHN). Results The present study indicated improvement in micro-hardness levels for both groups although experimental group showed more enhancement. The mean baseline VHN for control group was 498.6, 500.4 for post-immersion, and 503.5 for post-brushing. The mean baseline VHN for experimental group was 448.7, 531 for post-immersion, and 610.3 for post-brushing. Statistically significant differences (p < 0.05) were observed when post-brushing VHN values of both groups were compared and also within the experimental group when post-brushing values were compared with baseline values. Statistical Analysis Wilcoxon signed-rank test was applied for the evaluation of pre- and post-exposure hardness values. Level of significance was ≤0.05. Conclusion The exposure of enamel to TQ led to an improvement in its micro-hardness levels. Further studies are required to understand the mechanism of action of TQ on human tissues.

scholarly journals Microbiological study of the efficiency of root canal treatment with Er:yYAG laser

2020 ◽  
Vol 8 (4) ◽  
pp. 11-16 ◽  
Author(s):  
S. N. Razumova ◽  
A. S. Brago ◽  
H. B. Barakat ◽  
Yu. S. Kozlova ◽  
E. V. Velichko ◽  
...  
Keyword(s):  
Drug Treatment ◽  
Laser Irradiation ◽  
Root Canal ◽  
Clinical Studies ◽  
In Vitro Study ◽  
Root Canal Treatment ◽  
Control Group ◽  
Erbium Laser ◽  
Experimental Group

Mechanical and drug treatment of the root canal are the most important components of the success of endodontic treatment. This work presents the results on the effectiveness of the root canal disinfection using an erbium laser in combination with a 17% solution of ethylene diaminetetraacetic acid (EDTA) in vitro and in clinical studies. An in vitro study was carried out on removed intact teeth infected with strains of Enterococcus faecalis, Streptococcus sanguinis, and Candida albicans. An experimental group of teeth was treated medically according to a standard protocol using 3% solution of sodium hypochlorite and 17% solution of EDTA followed by irradiation with erbium laser. The control group was treated similarly but without laser irradiation. The treatment effectiveness was evaluated by the reduction of CFU. After the treatment, in the control group after the mechanical and drug treatment, the CFU amount lowered by 4 times, while the complete sterilization was observed in the experimental group. The clinical studies included two groups of 35 patients each diagnosed with chronic periodontitis. In the experimental group of patients, at the final stage, treatment with erbium laser for 1 min using endodontic piece (40 mJ power, 2940 nm wavelength, 10 Hz pulse rate) and a 17% EDTA solution was performed before filling. Periodontitis treatment in the control group was carried out without the laser treatment. The control group of patients saw the reduction on CFU after the mechanical and drug treatment by 3–6 times, while the experimental group achieved the complete sterilization of the root canals. The obtained results prove that the modification of the root canal treatment by the inclusion of erbium laser irradiation is a promising direction in endodontics.

scholarly journals The application of MTA as apical plug for root canal obturation – in vitro study

2018 ◽  
Vol 65 (2) ◽  
pp. 71-77
Author(s):  
Ivan Matović ◽  
Dragan V. Ilić ◽  
Renata Petrović ◽  
Dejan Ostojić
Keyword(s):  
Root Canal ◽  
Gas Permeability ◽  
In Vitro Study ◽  
Modern Concept ◽  
Control Group ◽  
Root Canal Obturation ◽  
Root Canals ◽  
Experimental Group

Summary Introduction Prognosis of endodontically treated tooth is directly correlated to the quality of apical obturation. Modern concept of endodontics in particular way points out the quality of apical filling. The aim of this study was to assess the quality of root canal obturation with MTA apical plug using the method of gas (argon) penetration. Material and Methods Sixty-six freshly extracted single rooted (single canal) teeth were divided into the two experimental (30 teeth) and one control group (6). All canals were instrumented using hand and rotary files in step-back technique and copious irrigation of 1%NaOCl. In the first experimental group teeth were obturated using different sealers: Gutta Flow (Roeko), AH Plus (DeTrey), Acroseal (Septodont) and mono gutta-percha cone (10 canals each). In the second experimental group obturation involved 3 mm of MTA-Angelus apical plug while the remaining canal space was filled with the same three sealers as in the first group. The rate of gas permeability by Leak detector-Edwards LD 416 was measured in all teeth. Results The best seal was found in teeth obturated with Gutta Flow and MTA plug with average diffusion rate of 264.4 sec while the worst quality of obturation was found with Acroseal (178.5 sec-the highest gas permeability). All samples with MTA plug exhibited significantly lower leakage than the samples filled without MTA apical plug. Conclusion Root canals filled with MTA apical plug exhibited statistically significant lower gas permeability in comparison to the ones filled with sealer and guttapercha cones only.

Does Addition of Propolis to Glass Ionomer Cement Alter its Physicomechanical Properties? An In Vitro Study

2017 ◽  
Vol 41 (1) ◽  
pp. 62-65 ◽  
Author(s):  
P Subramaniam ◽  
KL Girish Babu ◽  
G Neeraja ◽  
S Pillai
Keyword(s):  
Compressive Strength ◽  
Testing Machine ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
Physicomechanical Properties ◽  
Control Group ◽  
Glass Ionomer ◽  
Experimental Group ◽  
Ionomer Cement

Propolis is a natural resinous substance produced by honey bees. The antimicrobial effects of glass ionomer cement have been shown to improve with the addition of propolis; however its effect on the physicomechanical properties of the cement is not known. Aim: The purpose of this study was to evaluate the compressive strength and solubility of conventional restorative glass ionomer cement following the addition of propolis. Study design: Twenty half cylindrical samples were prepared with conventional restorative glass ionomer cement formed the control group. Another twenty samples were prepared with propolis added to conventional restorative glass ionomer cement formed the experimental group. The compressive strength was assessed using universal testing machine. To assess solubility, the samples were immersed in deionised water at room temperature, for 7 days. The solubility was measured as a difference in the weight of the sample; prior to immersion and following immersion at the end of each day. Results: The control group had a significantly higher mean compressive strength of 146.26 Mpa as compared to the experimental group (135.06 Mpa). The solubility between the groups was significant. Conclusion: In comparison to the control group, incorporation of propolis to conventional restorative glass ionomer cement decreased the compressive strength significantly. The solubility of the cement in the experimental group increased significantly over 7day period as compared to the control group.

scholarly journals HMU fluorinze mouthwash enhances enamel remineralization: An in vitro study

2019 ◽  
Vol 7 (24) ◽  
pp. 4319-4323
Author(s):  
Vo Truong Nhu Ngoc ◽  
Kimberly Mathieu Coulton ◽  
Nguyen Thu Tra ◽  
Nguyen Ha My ◽  
Phi Thi Quynh Huong ◽  
...  
Keyword(s):  
Statistical Significance ◽  
In Vitro Study ◽  
Control Group ◽  
Third Molars ◽  
Nail Polish ◽  
Enamel Structure ◽  
Diagnodent Pen ◽  
Group B ◽  
Experimental Group

BACKGROUND: Fluoride therapy has long been used extensively to prevent dental caries. Fluoride appears in variety of dental care products such as mouthrinse, dentifrice, gel, etc. HMU fluorinze is the first mouthwash containing fluoride in Vietnam. AIM: This research was conducted to evaluate the efficacy of HMU Fluorinze mouthwash on remineralizing enamel in laboratory conditions. METHODS: 20 third molars teeth were cleaned and covered with nail polish , except for a 3x3 mm square on their buccal surfaces. These teeth went through two steps: demineralization using Coke and remineralization for 20 days: 1) using standard calcifying solution (control group) and 2) using standard calcifying solution + HMU Fluorinze mouthwash 2 times/day (experimental group). The mineralization index of enamel structure after demineralization and remineralization was assessed by DIAGNOdent pen 2190. RESULTS: The mineralization indexes of the control group and experimental group at baseline were 3.65 ± 0.76 and 3.35 ± 0.64, after demineralization were in turn of 21.78 ± 4.48 and 20.25 ± 2.26; and after remineralization were 6.30 ± 1.03 and 3.90 ± 1.24. The different figures  between the two groups after remineralization shows statistical significance (p<0.01). Group B using HMU fluorinze mouthwash after 20 days did not differ from the original results (p = 0.272), in contrast with the control group (p<0.01). CONCLUSIONS: HMU fluorinze mouthwash has better mineralization effect than standard calcifying solution.

Reverse torque values of abutment screws after dynamic loading: The effects of sealant agents and the taper of conical connections

2020 ◽  
Author(s):  
Arda Ozdiler ◽  
suleyman dayan ◽  
Burc Gencel ◽  
Gulbahar Isık-Ozkol
Keyword(s):  
Dynamic Loading ◽  
Antibacterial Agent ◽  
In Vitro Study ◽  
Control Group ◽  
Silicone Sealant ◽  
Reverse Torque ◽  
The Stability ◽  
Torque Values ◽  
Chlorhexidine Gel

This in vitro study evaluated the influence of taper angles on the internal conical connections of implant systems and of the application of chlorhexidine gel as an antibacterial agent or a polyvinyl siloxane (PVS) sealant on the reverse torque values of abutment screws after dynamic loading. The current study tested four implant systems with different taper angles (5.4°, 12°, 45°, and 60°). Specimens were divided into three groups: control (neither chlorhexidine gel filled nor silicone sealed), 2% chlorhexidine gel-filled or silicone-sealed group, and group subjected to a dynamic load of 50 N at 1 Hz for 500,000 cycles prior to reverse torque measurements. Quantitative positive correlation was observed between the taper angle degree and the percentage of tightening torque loss. However, this correlation was significant only for the 60° connection groups except in the group in which a sealant was applied ( p = 0.013 for the control group, p = 0.007 for the chlorhexidine group). Percentages of decrease in the torque values of the specimens with silicone sealant application were significantly higher compared with both the control and chlorhexidine groups ( p = 0.001, p = 0.002, p = 0.001, and p = 0.002, respectively, according to the increasing taper angles); the percentage of decrease in torque values due to chlorhexidine application was statistically insignificant when compared with the control group. The application of gel-form chlorhexidine as an antibacterial agent does not significantly affect the stability of the implant–abutment connection under dynamic loads. PVS sealants may cause screw loosening under functional loads.

Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Rathika Rai ◽  
M. A. Easwaran ◽  
K. T. Dhivya
Keyword(s):  
Sodium Hypochlorite ◽  
In Vitro Study ◽  
Control Group ◽  
Immersion Method ◽  
Significant Difference ◽  
Surface Irregularities ◽  
Disinfectant Solution ◽  
Surface Detail ◽  
Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

scholarly journals Pomegranate Peel Extract Is a Potential Alternative Therapeutic for Giardiasis

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 705
Author(s):  
Asmaa M. El-Kady ◽  
Iman A. M. Abdel-Rahman ◽  
Samer S. Fouad ◽  
Khaled S. Allemailem ◽  
Taghrid Istivan ◽  
...  
Keyword(s):  
Diarrheal Disease ◽  
Apoptotic Cell ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Control Group ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Multiple Drugs ◽  
Peel Extract

Giardiasis is a major diarrheal disease affecting approximately 2.5 million children annually in developing countries. Several studies have reported the resistance of Giardia lamblia (G. lamblia) to multiple drugs. Therefore, identifying an effective drug for giardiasis is a necessity. This study examined the antiparasitic effect of Punica granatum (pomegranate) and evaluated its therapeutic efficacy in rats infected with G. lamblia. In vitro study showed high efficacy of pomegranate peel ethanolic extract in killing G. lamblia cysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number of G. lamblia cysts and trophozoites in feces and intestine respectively. Interestingly, the number of G. lamblia trophozoites and cysts were significantly lower in the pomegranate extract-treated group compared to the metronidazole-positive control group. Moreover, pomegranate extract treatment significantly induced nitric oxide (NO) and reduced serum IL-6 and TNF-α, compared to infected untreated rats. Histological and scanning electron microscopy (SEM) examination of the jejunum and duodenum of pomegranate extract-treated animals confirmed the antiparasitic effect of the extract, and demonstrated the restoration of villi structure with reduction of villi atrophy, decreased infiltration of lymphocytes, and protection of intestinal cells from apoptotic cell death. In conclusion, our data show that the pomegranate peel extract is effective in controlling G. lamblia infections, which suggests that it could be a viable treatment option for giardiasis.

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