scholarly journals Pomegranate Peel Extract Is a Potential Alternative Therapeutic for Giardiasis

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 705
Author(s):  
Asmaa M. El-Kady ◽  
Iman A. M. Abdel-Rahman ◽  
Samer S. Fouad ◽  
Khaled S. Allemailem ◽  
Taghrid Istivan ◽  
...  
Keyword(s):  
Diarrheal Disease ◽  
Apoptotic Cell ◽  
In Vitro Study ◽  
Ethanolic Extract ◽  
Control Group ◽  
Pomegranate Peel ◽  
Pomegranate Peel Extract ◽  
Multiple Drugs ◽  
Peel Extract

Giardiasis is a major diarrheal disease affecting approximately 2.5 million children annually in developing countries. Several studies have reported the resistance of Giardia lamblia (G. lamblia) to multiple drugs. Therefore, identifying an effective drug for giardiasis is a necessity. This study examined the antiparasitic effect of Punica granatum (pomegranate) and evaluated its therapeutic efficacy in rats infected with G. lamblia. In vitro study showed high efficacy of pomegranate peel ethanolic extract in killing G. lamblia cysts as demonstrated by eosin vital staining. We showed that treating infected rats with pomegranate extract resulted in a marked reduction in the mean number of G. lamblia cysts and trophozoites in feces and intestine respectively. Interestingly, the number of G. lamblia trophozoites and cysts were significantly lower in the pomegranate extract-treated group compared to the metronidazole-positive control group. Moreover, pomegranate extract treatment significantly induced nitric oxide (NO) and reduced serum IL-6 and TNF-α, compared to infected untreated rats. Histological and scanning electron microscopy (SEM) examination of the jejunum and duodenum of pomegranate extract-treated animals confirmed the antiparasitic effect of the extract, and demonstrated the restoration of villi structure with reduction of villi atrophy, decreased infiltration of lymphocytes, and protection of intestinal cells from apoptotic cell death. In conclusion, our data show that the pomegranate peel extract is effective in controlling G. lamblia infections, which suggests that it could be a viable treatment option for giardiasis.

scholarly journals Pomegranate Peel Extract Polyphenols Attenuate the SARS-CoV-2 S-glycoprotein Binding Ability to ACE2 Receptor: In Silico and In Vitro Studies

2021 ◽  
pp. 105145
Author(s):  
Relja Suručić ◽  
Maja Travar ◽  
Miroslav Petković ◽  
BiljanaTubić ◽  
Miloš P. Stojiljković ◽  
...  
Keyword(s):  
In Silico ◽  
In Vitro Studies ◽  
Pomegranate Peel ◽  
Binding Ability ◽  
Pomegranate Peel Extract ◽  
Peel Extract

scholarly journals Effectiveness of a pomegranate peel extract (PGE) in reducing Listeria monocytogenes in vitro and on fresh-cut pear, apple and melon

2020 ◽  
Vol 246 (9) ◽  
pp. 1765-1772
Author(s):  
I. Belgacem ◽  
L. Schena ◽  
N. Teixidó ◽  
F. V. Romeo ◽  
G. Ballistreri ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Pomegranate Peel ◽  
Fresh Cut ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Development and Evaluation of a Novel Oral Mucoadhesive Ointment Containing Pomegranate Peel Extract as an Adjuvant for Oral Hygiene of Dogs

2020 ◽  
Vol 37 (3) ◽  
pp. 133-140
Author(s):  
Thais M. Silva ◽  
Thays C. A. Bolzan ◽  
Marcos S. Zanini ◽  
Taiana Alencar ◽  
Winner D. Rodrigues ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Oral Hygiene ◽  
Diffusion Method ◽  
Pomegranate Peel ◽  
Ointment Formulation ◽  
Microbiological Testing ◽  
Herbal Therapies ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Herbal therapies are used worldwide to treat a variety of health conditions, including dental conditions in veterinary medicine. In this context, the use of medicinal plant-based formulations as potential therapeutics and preventatives in veterinary dentistry is worth highlighting. The objective of the present study was to develop a mucoadhesive ointment formulation, named orabase, that contained pomegranate extract for use in the oral cavity of dogs, with the aim of improving their oral hygiene. The hydroalcoholic extracts of pomegranate peels was incorporated into the orabase in 3 different concentrations. The formulations were subjected to in vitro microbiological testing by a modified disc-diffusion method to study the susceptibility of microorganisms collected from the oral cavities of the dogs. The samples were taken from the buccal mucosa of dogs having the same management and diet. The most effective formulation was submitted to physicochemical tests to evaluate the functionality of the product, namely pH, swelling index, spreadability, and mechanical properties (hardness, cohesiveness, and adhesiveness). The formulation containing 25.0% w/w of the extract was considered most suitable for the intended use as it showed antiseptic activity and demonstrated a swelling index of approximately 35% in the first 20 minutes of the test, high spreadability, and suitable mechanical properties. The results suggest that the product obtained from pomegranate peel extract is a viable option for use to improve oral hygiene, helping to reduce the bacterial component of dental plaque in dogs.

scholarly journals The Antimicrobial Effect of Pomegranate Peel Extract versus Chlorhexidine in High Caries Risk Individuals Using Quantitative Real-Time Polymerase Chain Reaction: A Randomized Triple-Blind Controlled Clinical Trial

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Benoy Jacob ◽  
Nivedhitha Malli Sureshbabu ◽  
Manish Ranjan ◽  
Aishwarya Ranganath ◽  
Riluwan Siddique
Keyword(s):  
Clinical Trial ◽  
Control Group ◽  
Caries Risk ◽  
Chain Reaction ◽  
Pomegranate Peel ◽  
Oral Rinse ◽  
High Caries Risk ◽  
Polymerase Chain ◽  
Pomegranate Peel Extract ◽  
Peel Extract

The aim of the present study was to compare the antibacterial effectiveness of chlorhexidine and PPE oral rinse on S. mutans, Lactobacilli, and Veillonella, in clinical salivary samples of patients with advanced stages of dental caries at baseline and two and four weeks with PCR technique. This triple-blind randomized clinical trial involved 60 high caries risk adult patients, 19–59 years of age, randomly allocated into two groups of 30 subjects each. The intervention group received pomegranate peel extract mouthwash, whereas the control group received chlorhexidine mouthwash. Unstimulated pooled saliva was collected from the floor of the mouth before and after the intervention. The quantitative real-time polymerase chain reaction was employed to analyze the bacterial copies of each salivary sample at baseline and two and four weeks. The significance level was fixed at 5% (α = 0.05). Overall comparison of antimicrobial effectiveness across both groups revealed insignificant outcomes. The control group evinced a significant reduction in S. mutans between a specific time, i.e., baseline and 4 weeks ( p = 0.043 ). PPE oral rinse as a natural product or ecological alternative was effective in disrupting activity across all microorganisms tested in this triple-blind RCT; however, the nutraceutical, when compared to chlorhexidine, was not as effective against S. mutans.

scholarly journals Krim ekstrak kulit delima merah (Punica granatum) menghambat peningkatan jumlah melanin sama efektifnya dengan krim hidrokuinon pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Eva R. Siahaan ◽  
Wimpie Pangkahila ◽  
A A G P Wiraguna
Keyword(s):  
Guinea Pigs ◽  
Punica Granatum ◽  
Control Group ◽  
Cavia Porcellus ◽  
Pomegranate Peel ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Abstract: As the outer organ of the body, skin becomes the main target of environmental influences, particularly the UVB rays. Based on analysis of phytochemicals, red pomegranate peel extract contains antioxidants such as flavonoids, phenols, tannins, and vitamin C which are able to prevent increased skin melanin by inhibiting the actions of tyrosinase. This study was aimed to prove that application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in female guinea pigs (Cavia porcellus) exposed to UVB as well as to prove that the 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin. This was a true experimental study using post test only control group design. Subjects were divided into three groups, with 10 guinea pigs each group. Group 1, the control group, was treated with UVB exposure and basic cream. Group 2 was treated with UVB exposure and 4% hydroquinone cream. Group 3 was treated with UVB and 8% red pomegranate peel extract cream. A total of 390 mJ/cm UVB dosage was given for 2 weeks. Histopahtologic slides were stained with Masson-Fontana. The amount of melanin area colored in black was evaluated by the percentage of pixel areas of melanin compared with pixel areas of all epidermal tissues. The results showed that the highest amount of melanin was in group 1 (18.13±4.76%), followed by group 3 (1.876±0.73%), and group 2 as the lowest (0.67±0.291%). There was a significant difference between control group and group 2 as s well as group 3 (P <0.05). There was no significant difference between group 2 and group 3 (P >0.05). Conclusion: Application of 8% red pomegranate peel extract (Punica granatum) cream could prevent the increase of skin melanin in guinea pigs (Cavia porcellus) exposed to UVB. The 8% red pomegranate peel extract cream was as effective as 4% hydroquinone cream in preventing increased skin melanin.Keywords: red pomegranate peel extract, melanin, skin, UVBAbstrak: Sebagai organ terluar dari tubuh, kulit menjadi target utama dari pengaruh lingkungan, terutama oleh sinar UVB. Berdasarkan analisis fitokimia, ekstrak kulit delima merah memiliki kandungan antioksidan seperti flavonoid fenol, tanin, dan vitamin C yang mampu mencegah peningkatan jumlah melanin dengan cara menghambat kerja enzim tirosinase. Penelitian ini bertujuan untuk membuktikan efek pemberian krim ekstrak kulit delima merah (Punica granatum) 8% dalam menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB dan krim ekstrak kulit delima merah (Punica granatum) 8% memiliki efektivitas yang sama dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin tersebut. Jenis penelitian ialah eksperimental murni dengan post test only control group design. Subyek penelitian dibagi menjadi tiga kelompok, masing-masing berjumlah 10 ekor marmut betina. Kelompok 1 (kontrol) diberikan pajanan sinar UVB dan krim dasar; kelompok 2 diberikan paparan sinar UVB dan krim hidrokuinon 4%; dan kelompok 3diberikan pajanan sinar UVB dan krim ekstrak kulit delima merah 8%. Dosis total UVB 390 mJ/cm2 yang diberikan selama 2 minggu. Sediaan histopatologik jaringan kulit menggunakan pewarnaan Masson-Fontana. Jumlah melanin dihitung dengan persentase pixel luas area melanin dibandingkan pixel seluruh jaringan epidermis. Hasil penelitian menunjukkan bahwa jumlah melanin paling tinggi terdapat pada kelompok 1 (18,13±4,76%), diikuti kelompok 3 (1,876±0,73%), dan paling rendah pada kelompok 2 (0,67±0,291%). Terdapat perbedaan bermakna antara kelompok 1 dengan kelompok 2 dan 3 (P <0,05) sedangkan antara kelompok 2 dan 3 tidak berbeda bermakna dalam mencegah peningkatan jumlah melanin (P >0,05). Simpulan: Krim ekstrak kulit delima merah (Punica granatum) 8% dapat menghambat peningkatan jumlah melanin pada kulit marmut (Cavia porcellus) betina. Krim ekstrak kulit delima merah (Punica granatum) 8% sama efektif dengan krim hidrokuinon 4% dalam menghambat peningkatan jumlah melanin pada kulit marmut.Kata kunci: kulit delima merah, melanin, kulit, UVB, marmut betina.

scholarly journals Effects of Pomegranate Peel (Punica granatum L.) Extract as an Anthelmintic

2017 ◽  
Vol 1 (5) ◽  
Author(s):  
Monica Amelia ◽  
Diana K Jasaputra ◽  
Rita Tjokropranoto
Keyword(s):  
Rural Areas ◽  
Ascaris Suum ◽  
Pomegranate Peel ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Pomegranate Peel Extract ◽  
Peel Extract ◽  
Anthelmintic Effect

Helminths infections caused by Soil-Transmitted Helminths (STH) are found in manypeople living in developing countries, especially in rural areas. People often use pomegranate asan anthelmintic. The aim of this research is to find out the effects of pomegranate peel (Punicagranatum L.) as an anthelmintic to female Ascaris suum in vitro. The research on the effects ofpomegranate peel extract has been conducted on 900 female Ascaris suum in vitro. Ascaris suumare divided into 5 groups, group I: pomegranate peel extract of dose 25%, group II: pomegranatepeel extract of 50%, group III: pomegranate peel extract of 75%, group IV: 0.9% NaCl as anegative control, and group V is given mebendazole 0.5% as a positive control. The meanpercentage of dead worms in group I is 39%, in group II 61%, while in group III 82%, but itspotential is lower than mebendazole, which kills 100%. The treatment using pomegranate peelextract of 25%, 50% and 75% respectively has significant differences with p < 0.05 againstnegative control (NC) using a 0.9% NaCl. The research concludes that the pomegranate peelextract has an anthelmintic effect against Ascaris suum females in vitro.Keywords: pomegranate peel extract, anthelmintic effect, in vitro

scholarly journals AMELIORATIVE EFFECTS OF PUNICA GRANATUMON RENAL TUBULAR NECROSIS INDUCED BY NANDROLONE DECANOATE IN MICE MODEL

2021 ◽  
Vol 71 (4) ◽  
pp. 1248-51
Author(s):  
Humaira Ali ◽  
Liaqat Ali ◽  
Sadia Shaukat ◽  
Maria Yousaf ◽  
Faiza Umbreen ◽  
...  
Keyword(s):  
Body Weight ◽  
Control Group ◽  
Nandrolone Decanoate ◽  
Oral Gavage ◽  
Pomegranate Peel ◽  
Tubular Necrosis ◽  
Group A ◽  
Renal Tubular ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Objective: To observe the effects of nandrolone decanoateon renal tubules and its protection by punicagranatum (pomegranate) in mice. Study Design: Lab based experimental study. Place and Duration of Study: One year in Anatomy department, Army Medical College, Rawalpindi, in association with National Institution of Health, Islamabad. Methodology: Forty healthy BALB/c mice of both sexes with weight range of 2 5-30gms were equally disseminated into four groups, A as control while B, C and D as trial groups. Three trial groups were inoculated Nandrolone Decanoate 1mg per 100gm of body weight, through intramuscular injections in the hind limb, once weekly for eight weeks. Pomegranate nector was administered in animals of trial group C (3ml per kg of body weight) en route oral gavage tube daily, whereas animals in trial group D was administered pomegranate peel extract (200mg per kg body weight) via oral gavage tube daily for eight weeks. Evaluation of the outcomes of trial groups B, C & D was done amongst them and with control group A. Results: In nandrolone decanoate injected experimental group B, tubular necrosis were appreciated in comparison with control group A (p˂ 0.001), and showed statistical improvement when evaluation was done with pomegranate nectar and pomegranate peel extract treated trial groups C (0.001) and D (p=0.001), correspondingly. Conclusion: Punicagranatum in two forms, as nectar and peel extract, has almost identical curative effects on steroid administered renal tubular mutilation.

Phenolic-rich Pomegranate Peel Extract: In Vitro, Cellular, and In Vivo Activities for Skin Hyperpigmentation Treatment

Planta Medica ◽  
2020 ◽  
Vol 86 (11) ◽  
pp. 749-759
Author(s):  
Mayuree Kanlayavattanakul ◽  
Wichayada Chongnativisit ◽  
Puxvadee Chaikul ◽  
Nattaya Lourith
Keyword(s):  
Sinapic Acid ◽  
Inhibitory Effect ◽  
Dermal Fibroblasts ◽  
Pomegranate Peel ◽  
Double Blind ◽  
Skin Hyperpigmentation ◽  
Pomegranate Peel Extract ◽  
Skin Lightening ◽  
Peel Extract

AbstractThe pomegranate phenolics are reported to have cutaneous benefits and to be effective in treating skin disorders, including hyperpigmentation. In this context, a preparation method was developed by which to obtain phenolic-rich pomegranate peel extract. Sinapic acid was presented as the major pomegranate peel phenolics, followed by gallic and ellagic acids, and 4 additional phenolics. The extract exhibited strong antioxidant activity with an in vitro tyrosinase inhibitory effect. The skin hyperpigmentation treating potency was confirmed by the suppression of cellular melanogenesis through tyrosinase and TRP-2 inhibitions as examined in the B16F10 melanoma cells. Cellular antioxidant and proliferative activities of the extract toward human dermal fibroblasts were evidenced, as well as an inhibitory effect against MMP-2. The extract was developed into the stable serum and mask. The products were proved to be non-irritated in 30 Thai volunteers participating in a single application closed patch test. A split-face, randomized, double-blind, placebo-controlled test of the skin lightening effect was evaluated in the 30 volunteers over 28 consecutive daily treatments and monitored by the Mexameter MX 18. The active serum and mask were better in facial skin lightening efficacy than the placebo (p < 0.005). That was in accordance with the sensory evaluation scored by the volunteers. Phenolic-rich pomegranate peel extract is evidenced as a safe herbal derived material promising for skin hyperpigmentation treatment. Supportive information regarding chemical and biological profiles is presented with the confirmed safety and cutaneous benefits in volunteers.

scholarly journals Determination of antioxidant and antimicrobial effects of pomegranate peel extract in trout burgers stored at cold temperatures (4±1°C)

2020 ◽  
Vol 37 (4) ◽  
pp. 415-422
Author(s):  
Ilknur Uçak
Keyword(s):  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Control Group ◽  
Pomegranate Peel ◽  
Cold Temperatures ◽  
Pomegranate Peel Extract ◽  
Peel Extract ◽  
Total Yeast

Recently, it has become a common practice to utilize food processing waste as natural additives in food products by evaluating their antioxidant and antimicrobial activities. Pomegranate peel is also an important by-product involved in the pomegranate processing industry and has strong antioxidant and antimicrobial properties. Therefore, the aim of this study is to examine the quality changes of trout burgers prepared with pomegranate peel extract during the refrigerated (4±1°C) storage. For this purpose, peroxide value, thiobarturic acid (TBARs), total aerobic mesophilic bacteria (TAMB) count, total psyschophilis bacteria (TPB) count, total coliform bacteria, total yeast-mold, total lactic acid bacteria (LAB) count and sensory quality parameters were evaluated in trout burgers. Peoxide value was found to be 2.75 meq O2/kg at the beginning with increasing in all groups and were observed significantly lower in the groups prepared with pomegranate peel extract than the control group (24.85 meq O2/kg). TBARs value of trout fillet was 0.07 mg MDA/kg and increased in all groups until the end of storage. The lowest values compared to the control group were found in the group prepared with 1% pomegranate peel extract. At the end of storage, TBARs values of the control, groups enriched with 0.5% and 1% pomegranate peel extract reached 2.78, 2.32 and 2.25 mg MDA/kg, respectively. It has been determined that pomegranate peel extract has a suppressive effect on TAMB, TPB, total coliform, total yeast-mold and LAB growth in trout burgers. As a result of sensory evaluations, pomegranate peel extract has been observed to extend the shelf life of trout burgers by 6 days compared to the control group. All these data revealed that pomegranate peel extract can be used as an alternative natural additive in trout burgers.

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