Asparaginase Like 1 Predicts Unfavourable Prognosis and Facilitates Gastric Cancer Progression Through Inhibits GSK3-β
Abstract Background:ASRGL1 plays critical roles in various biological processes and pathologic conditions,including cancer. However, the prognostic importance and biologic functions of ASRGL1 in gastric cancer (GC) are still unclear.Methods:qRT-PCR, western blot and immunohistochemistry analyses were used to determine ASRGL1 expression in GC samples and cell lines. The clinical significance of ASRGL1 was assessed in 100 patients with GC. A series of functional experiments were performed to explore the role and molecular mechanism of ASRGL1 on GC progression. Results:ASRGL1 was upregulated in GC tissues and cell lines. High ASRGL1 expression was closely correlated with aggressive clinicopathological features, poor clinical outcomes and recurrence of GC patients. Moreover, silencing ASRGL1 in AGS cells significantly inhibited cell proliferation, migration, invasion, whereas overexpression of ASRGL1 significantly enhanced the above abilities of BGC-823 cells. Further mechanism study indicated that these phenotypic changes were mediated by PI3K/AKT and WNT signaling. Finally, we proved that ASRGL1 exerted its tumor-promoting effect by interacting with GSK3-β.Conclusions:ASRGL1 is up regulated in gastric cancer, while it promotes the GC cell proliferation, migration and invasion via interacting with GSK3-β . At the same time it also serves as a potential prognostic factor in patients with GC. ASRGL1 can be used as a new target for diagnosis and treatment of gastric cancer.