Effective Biomarkers and Therapeutic Targets of Nerve-Immunity Interaction in The Treatment of Depression: A Integrated Analysis of miRNA-mRNA Regulatory Networks
Abstract Background: Major depressive disorder (MDD) is an emotional disorder that has a negative effect on patients' studies and daily lives. A great number of studies have found that miRNAs play an important role in the development of MDD and that they can be used as a biomarker for the diagnosis and treatment of MDD. However, there have been few investigations on nerve-immunity interaction therapy for MMD patients' brains.Methods: We attempted to evaluate MDD in the gene expression matrix database and miRNAs in plasma samples from healthy controls using bioinformatics methods. Four plasma miRNAs (DE-miRNAs) samples were found from MDD patients. Funrich planned the transcription factors and target genes of miRNAs, and the enrichment of TF and GO was examined. The intersecting mRNAs were discovered by comparing the various expressions of the projected target genes and 5 mRNAs (DE-mRNAs) samples. In the end, 34 DE-miRNAs, 386 DE-mRNAs, and 17 intersecting mRNAs were detected. Intersecting core genes were then investigated using GO and KEGG enrichment analysis to find the intersecting mRNA. Identify particular candidate genes and pathways in neurology and immunology that may be associated with MDD for further investigation.Results: We discovered 17 important HUB genes by the advance of a miRNA-mRNA network, and 5 HUB DE-MRNAs were derived following CytoNCA topology.Conclusion: Our findings from a comprehensive bioinformatics analysis of miRNAs and mRNAs in MDD show that DE-miRNAs like miR-338-3P and miR-206 may be excellent biomarkers and potential therapeutic targets for the treatment of MDD via nerve-immunity interaction.