872 PB 226 ENHANCED ROOT AND SHOOT GROWTH OF CHRYSANTHEMUM CUTTINGS PROPAGATED IN MEDIA INOCULATED WITH TRICHODERMA HARZIANUM

Trichoderma harzianum is a well-documented biocontrol agent that has been shown to enhance rooting of chrysanthemum `White Marble'. The objective of this research was to determine if T. harzianum would enhance rooting of hard-to-root chrysanthemum cuttings. Two hard-to-root cultivars (`Dark Bronze Charm' and `Golden Bounty') and two easy-to-root cultivars (`Davis' and `White Marble') were propagated in a 1:1 peat-perlite medium amended with T. harzianum at a rate of 0, 5, or 25g/kg medium. Measurements were taken 7, 14, and 21 days after insertion of the cuttings into the medium. Interactions occurred between rate of amendment and day of measurement for some variables measured. However, overall there was increased root fresh and dry weight of all cultivars when T. harzionum rates were 5 or 25 g/kg medium. Increased root fresh and dry weight occurred on days 14 and 21 for most cultivars. Root fresh and dry weight increased with increasing rate of amendment on day 14 but there was no difference between the 5 and 25 g/kg rates on day 21. Shoot fresh weight was increased with 5 or 25 g/kg each measurement day for all cultivar except `White Marble' and shoot length was increased with 25g/kg for all cultivars.

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Enhanced Root and Shoot Growth of Chrysanthemum Cuttings Propagated with the Fungus Trichoderma harzianum

HortScience ◽

10.21273/hortsci.30.3.496 ◽

1995 ◽

Vol 30 (3) ◽

pp. 496-498 ◽

Cited By ~ 6

Author(s):

Amy J. MacKenzie ◽

Terri Woods Starman ◽

Mark T. Windham

Keyword(s):

Trichoderma Harzianum ◽

Shoot Growth ◽

Dry Weight ◽

Soilborne Pathogens ◽

White Marble ◽

Shoot Dry Weight ◽

Growing Medium ◽

Root And Shoot Growth

Trichoderma harzianum Rifai, a fungus that controls soilborne pathogens, can enhance growth of several vegetable and floriculture crops. Zero, 5, or 25 g of T. harzianum (isolate T-12) peat–bran amendment was added per kilogram medium in an effort to enhance the rooting of four chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] cultivars, two considered easy to root (`Davis' and `White Marble') and two considered hard to root (`Dark Bronze Charm' and `Golden Bounty'). Adding the T. harzianum amendment at both rates tested increased root and shoot fresh weights during 21 days of rooting, relative to the control. Supplementary treated cuttings were transplanted into nontreated growing medium after 21 days. Midway between transplant to flowering, increases in height, shoot dry weight, and root fresh and dry weight were detected in `Dark Bronze Charm' with T-12, relative to the control; increases in height, shoot fresh and dry weight, and number of nodes were detected in `Golden Bounty' with T-12. By this time, there were no detectable differences in `Davis' or `White Marble'.

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Growth Assessment of Tropical Sugarbeet as Influenced by Spacing

Bangladesh Agronomy Journal ◽

10.3329/baj.v23i2.52452 ◽

2021 ◽

Vol 23 (2) ◽

pp. 51-58

Author(s):

MAT Sohel ◽

MAE Hossain ◽

HP Roy ◽

SM Reza ◽

FH Shanta ◽

...

Keyword(s):

Root Length ◽

Block Design ◽

Dry Weight ◽

Germination Percentage ◽

Research Field ◽

Shoot Length ◽

Fresh Weight ◽

Shoot Fresh Weight ◽

Number Of Leaves ◽

Maximum Root

The experiment was carried out at the research field of Agronomy and Farming Systems Division, Bangladesh Sugarcrop Research Institute (BSRI), Ishurdi, Pabna during 2012-2013 to determine the most suitable spacing for sugarbeet cultivation in Bangladesh. The experiment was conducted with nine spacing viz. 50 cm × 20 cm, 60 cm × 20 cm, 70 cm × 20 cm, 50 cm × 25 cm, 60 cm × 25 cm, 70 cm × 25 cm, 50 cm × 30 cm, 60 cm × 30 cm and 70 cm × 30 cm in a randomized complete block design with three replications. The effects of spacing on sugarbeet plantation were observed on growth and growth contributing components (germination percentage, number of leaves plant-1, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, crop growth rate) of sugarbeet. The highest germination percentage (95.67%), number of leaves plant-1 (34.33) at 30 DAS, shoot length (54.07 cm) at 120 DAS, root fresh weight (969.47 g plant-1) at 150 DAS, shoot fresh weight (752.47 g plant-1) at 120 DAS and other growth contributing parameters were obtained with the spacing 70 cm × 30 cm. However, the maximum root length (38.97 cm) was obtained with 50 cm × 20 cm spacing. It was concluded that the wider spacing promoted the growth of individual beet, though the optimum spacing for maximum root growth of sugarbeet was 50 cm × 20 cm. Bangladesh Agron. J. 2020, 23(2): 51-58

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The Influence of Salinity on Seedling Growth of Some Pumpkin Varieties Used as Rootstock

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4118349 ◽

2013 ◽

Vol 41 (1) ◽

pp. 219 ◽

Cited By ~ 9

Author(s):

Rana KURUM ◽

Kamile ULUKAPI ◽

Köksal AYDINŞAKİR ◽

Ahmet Naci ONUS

Keyword(s):

Seedling Growth ◽

Root Length ◽

Dry Weight ◽

Shoot Length ◽

Stem Length ◽

Fresh Weight ◽

Shoot Fresh Weight ◽

Root Dry Weight ◽

Salinity Levels ◽

Plant Length

In this study, the effects of different salinity levels (0, “control”, 2, 4, 8, and 12 dS m-1) on seedling growth of Obez, RS 841 and Ferro F1 pumpkin varieties, widely used around the world as rootstock, were investigated. Seedlings grown under saline conditions were investigated for plant main stem length, plant length, root length, shoot length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weights and ion concentrations (Ca++, K+, Na+) in the leaves of pumpkin varieties. The results revealed that root length, shoot length, root fresh weight, root dry weight, shoot fresh weight and shoot dry weights tend to decrease when the electrical conductivity of the solution is increased. Results indicated that these varieties responded different to some investigated parameters under saline conditions.

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Effects of salinity stress on chickpea (Cicer arietinum L.) landraces during early growth stage

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20173093 ◽

2017 ◽

Vol 3 (7) ◽

pp. 214 ◽

Cited By ~ 1

Author(s):

Seyoum Zawude ◽

Diriba Shanko

Keyword(s):

Salinity Stress ◽

Root Length ◽

Growth Stage ◽

Dry Weight ◽

Shoot Length ◽

Salt Tolerant ◽

Fresh Weight ◽

Shoot Fresh Weight ◽

Root Dry Weight ◽

Shoot Dry Weight

Background: Salinity is one of the most serious abiotic stresses for crop plant growth. Chickpea grows under a wide range of climatic conditions and highly sensitive to salt stress. To determine the most tolerant genotype to salinity stress, an experiment was done as factorial form under completely block design (CRD) with three replications.Methods: The experimental treatments were four NaCl salinity levels (0, 5dS/m, 10dS/m and 15dS/m) and five chickpea landraces (Dadi, Dido, Dida, Dimi and Soya). Results: Results indicated that significant was observed in root length, shoot length, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, seedling shoot and root reduction traits in stress conditions. Dimi, Dido and Dadi were showed that high reduction in root length, shoot length, root length, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, seedling root and shoot in salinity conditions, respectively. The ANOVA for landraces and their interaction was found to be highly significant at (p<0.001) and (p<0.05) with all parameters. Landraces Dimi, Dido and Dadi were found salt tolerant but Soya was highly salt sensitive during seedling growth stage. Shoot dry weight had the most positive and significant correlation with root dry weight (r =0.987**). Seedling shoot reduction depicted a negative and significant correlation with total dry matter (r =-0.734**).Conclusions: This study indicated that developing genetic variability by identifying salt tolerant landrace is one of the appropriate strategies used to overcome salinity problem in arid and semi-arid areas.

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Toxicity of Zinc on Growth of an Aquatic Macrophyte, Ipomoea Aquatica Forsk

Current World Environment ◽

10.12944/cwe.11.1.27 ◽

2016 ◽

Vol 11 (1) ◽

pp. 218-227 ◽

Cited By ~ 1

Author(s):

Laitonjam Chanu ◽

Abhik Gupta

Keyword(s):

Aquatic Ecosystems ◽

Shoot Growth ◽

Aquatic Macrophyte ◽

Growth Parameters ◽

Dry Weight ◽

Fresh Weight ◽

High Concentration ◽

Carotene Content ◽

Ipomoea Aquatica ◽

Root And Shoot Growth

The effects of different concentrations of Zn on growth of an aquatic macrophyte, Ipomoea aquatica Forsk. were studied. Fresh weight, dry weight, shoot length, root length, number of nodes, development of leaves, and chlorophyll and carotene contents were the different growth parameters considered. Toxicity symptoms like browning and decaying of roots could be observed in plants treated at 22.7 mg L-1 Zn as early as 3rd day of experiment while yellowing of older leaves appeared during the later period of exposure. High concentration of Zn (12.71 – 22.7 mg L-1) significantly inhibited the growth of plant while lower Zn concentrations up to 4.09 mg L-1 enhanced its growth. However, at 7.26 mg L-1 Zn the chlorophyll as well as total carotene content in leaf of I. aquatica were significantly reduced from that in control on 5th day of exposure and subsequently the reduction was observed in lower concentrations. Thus, I. aquatica can be employed in biomonitoring of Zn polluted aquatic ecosystems using root browning, root and shoot growth inhibition, and chlorophyll and total carotene contents as sensitive biomarkers.

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Effect of delivery method and population size of Trichoderma harzianum on growth response of unrooted chrysanthemum cuttings

Canadian Journal of Microbiology ◽

10.1139/w00-056 ◽

2000 ◽

Vol 46 (8) ◽

pp. 730-735 ◽

Cited By ~ 2

Author(s):

Amy J MacKenzie ◽

Bonnie H Ownley ◽

Terri W Starman ◽

Mark T Windham

Keyword(s):

Root Growth ◽

Trichoderma Harzianum ◽

Growth Promotion ◽

Dry Weight ◽

Delivery Method ◽

Shoot Height ◽

Fungal Propagules ◽

Root And Shoot Growth ◽

Improved Performance ◽

Kg Medium

In a previous study, addition of Trichoderma harzianum Rifai isolate T-12 to a propagative medium resulted in improved performance of chrysanthemum cuttings. However, root and shoot growth of one cultivar, 'Dark Bronze Charm', were more responsive to a lower (5 g T-12/kg medium) than higher (25 g T-12/kg medium) rate of fungal propagules, suggesting potential phytotoxicity at higher concentrations. The objectives of this study were to investigate higher rates of T-12 medium amendment for phytotoxicity, and to examine an alternative method of delivering the fungus to the propagative medium in order to obtain a more uniform response from cuttings. Isolate T-12 was added to the propagative medium as either a powdered peat-bran amendment (0, 5, or 50 g T-12 /kg medium) or as alginate prills (80 or 800 g T-12/kg medium). There were no differences among treatments on day seven, but by day 21, shoot fresh weight and heights were significantly greater for plants treated with prills at 800 g T-12/kg medium. Both prill treatments resulted in greater shoot height on day 14 and 21 than all other treatments, which were similar to controls. Amendment with T-12 powder at 50 g/kg increased root length, but 80 g/kg medium added as prills decreased root dry weight compared to the control. The highest rate of T-12 (800 g prills/kg medium) had no effect on root growth. This suggests that moderate, rather than high rates of T-12 are more effective in promoting rooting of unrooted chrysanthemum, and that there is a potential for phytotoxic effects on root growth with higher rates.Key words: Dendranthema X grandiflorum, plant growth promotion, plant propagation.

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Pre-Sowing Seed Treatments with Silicon Nano-Iron and Nano-Silicon Particles on Germination of Dragonhead

Plant Breeding and Seed Science ◽

10.1515/plass-2016-0019 ◽

2016 ◽

Vol 74 (1) ◽

pp. 99-107

Author(s):

Naser Sabaghnia ◽

Saeed Yousefzadeh ◽

Mohsen Janmohammadi ◽

Mehdi Mohebodini

Keyword(s):

Silicon Dioxide ◽

Root Length ◽

Dry Weight ◽

Germination Percentage ◽

Shoot Length ◽

Nano Particles ◽

Distilled Water ◽

Fresh Weight ◽

Shoot Fresh Weight ◽

Nano Silicon

Abstract Nanotechnology is an emerging field of science widely exploited in agriculture in recent years. In this investigation, application of nanotechnology in agriculture via application of some nano-particles (nano-iron and nano-silicon) have been investigated in seed priming of dragonhead. Seeds were subjected to pre-hydration treatments by factor nano-silicon dioxide as; (S1) 0 mM or distilled water, (S2) 1 mM concentration and (S3) 2 mM concentration and and factor nano-iron oxide as; (F1) 0 Mm or distilled water, (F2) 1 mM concentration and (F3) 2 mM concentration. Germination percent, root fresh weight, shoot fresh weight, root length, shoot length, dry weight of the seed residue, root dry weight and shoot dry weight were measured. Analysis of variance showed significant variation for the main effect of nano-silicon dioxide as well as nano-iron dioxide in root length and dry weight of the seed residue. The interaction effect of nano-silicon × nano-iron priming treatments were significant in all of the measured traits except germination percentage and root fresh weight. The highest germination percentage was recorded in S2-F3, S3-F1 and S3-F3 while the root fresh weight was high in S2-F3 and treatments S1-F1 following to S2-F3 and S3-F2 produced the highest shoot fresh weight. Also, S2-F3 has the highest root length (16.1 cm) and the highest shoot length (18.4 cm). The best treatment combination suitable for obtaining of high values of germination characteristics of dragon-head was identified as S2-F3 (1 mM nano-silicon dioxide plus 2 mM nano-iron dioxide).

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Effect of Repeated Short Interval Flooding Events on Root and Shoot Growth of Four Landscape Shrub Taxa

Journal of Environmental Horticulture ◽

10.24266/0738-2898-29.4.220 ◽

2011 ◽

Vol 29 (4) ◽

pp. 220-222

Author(s):

Kathryne J. Jernigan ◽

Amy N. Wright

Keyword(s):

Shoot Growth ◽

No Reduction ◽

Southeastern United States ◽

Short Interval ◽

Dry Weight ◽

Shoot Dry Weight ◽

Flooding Event ◽

Root And Shoot Growth ◽

Size Index ◽

Flooding Events

Abstract Research was conducted to screen four landscape shrub taxa for tolerance to repeated flooding events. Plants of Fothergilla × intermedia ‘Mt. Airy’ (dwarf witchalder), Ilex verticillata ‘Winter Red’ (winterberry), Clethra alnifolia ‘Ruby Spice’ (summersweet), and Viburnum nudum Brandywine™ (possumhaw) were flooded repeatedly over six weeks for 0 (non-flooded), 3, or 6 days with a draining period of 6 days between each flooding event. The experiment was repeated for a total of two runs. With the exception of F. × intermedia ‘Mt. Airy’, all taxa showed good visual quality and no reduction in root growth in either run, and effects on shoot growth were minimal. Size index of Clethra alnifolia ‘Ruby Spice’ was 27% higher in plants flooded for 0 or 3 days than in plants flooded for 6 days in run 1 only. Shoot dry weight of Ilex verticillata ‘Winter Red’ was actually 11% higher in plants flooded 6 days days than in plants flooded for 0 or 3 days in run 2. Size index of Viburnum nudum Brandywine™ increased with increasing flood length, and plants flooded for 6 days had a 9% higher SI than plants flooded for 0 days in run 1. With the exception of Fothergilla × intermedia L. ‘Mt. Airy’, all taxa appeared tolerant of and even thrived during flooding and would be appropriate shrub selections for a southeastern United States rain garden.

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In vitro screening of rice genotypes using polyethylene glycol under drought stress

Progressive Agriculture ◽

10.3329/pa.v27i2.29321 ◽

2016 ◽

Vol 27 (2) ◽

pp. 128-135 ◽

Cited By ~ 1

Author(s):

J Akte ◽

S Yasmin ◽

MJH Bhuiyan ◽

F Khatun ◽

J Roy ◽

...

Keyword(s):

Water Stress ◽

Drought Stress ◽

Water Content ◽

Relative Water Content ◽

Dry Weight ◽

Proline Accumulation ◽

Shoot Length ◽

Fresh Weight ◽

Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

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Biosynthesis and characterization of silver nanoparticles using Ochradenus arabicus and their physiological effect on Maerua oblongifolia raised in vitro

Scientific Reports ◽

10.1038/s41598-020-74675-9 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Hassan O. Shaikhaldein ◽

Fahad Al-Qurainy ◽

Mohammad Nadeem ◽

Salim Khan ◽

Mohamed Tarroum ◽

...

Keyword(s):

Silver Nanoparticles ◽

Chlorophyll Content ◽

Total Protein ◽

Dry Weight ◽

Shoot Length ◽

Antioxidant Enzyme Activities ◽

Fresh Weight ◽

Wide Range ◽

Shoot Number

Abstract Silver nanoparticles (AgNPs) are presently the most commonly generated engineered nanomaterials and are found in a wide range of agro-commercial products. The present study was designed to synthesize AgNPs biologically using Ochradenus arabicus leaves and investigate their effect on the morphophysiological properties of Maerua oblongifolia raised in vitro. Physicochemical methods (ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy were performed for characterization and for obtaining microphotographs of the AgNPs. Shoots of M. oblongifolia (2–3 cm) grown in Murashige and Skoog medium supplemented with different concentrations of AgNPs (0, 10, 20, 30, 40, or 50 mg L−1) were used. Following 6 weeks of in vitro shoot regeneration, the shoot number, shoot length, leaf number, fresh weight, dry weight, chlorophyll content, total protein, proline level, and antioxidant enzyme activities of the plants were quantified. We found that 20 mg L−1 AgNPs increased the shoot number, shoot length, fresh weight, dry weight, and chlorophyll content of the plants. The maximum total protein was recorded in plants that were administered the lowest dose of AgNPs (10 mg L−1), while high concentrations of AgNPs (40 and 50 mg L−1) increased the levels of proline and the enzymes superoxide dismutase and catalase. Our results indicate that green-synthesized AgNPs may be of agricultural and medicinal interest owing to their effects on plants in vitro.

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